Effect of radiation decontamination on drug-resistant bacteria
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1 Effect of radiation decontamination on drug-resistant bacteria Hitoshi ITO formerly Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Yahata-machi 935-6, Takasaki, Gunma Japan Summary More than 80% of food poisoning bacteria such as Salmonella are reported as antibiotic-resistant to at least one type antibiotic, and more than 50% as resistant to two or more. For the decontamination of food poisoning bacteria in foods, radiation resistibility on drug-resistant bacteria were investigated compared with drug-sensitive bacteria. Possibility on induction of drugresistant mutation by radiation treatment was also investigated. For these studies, type strains of Escherichia coli S2, Salmonella enteritidis YK-2 and Staphylococcus aureus H12 were used to induce drug-resistant strains with penicillin G. From the study of radiation sensitivity on the drug-resistant strain induced from E. coli S2, D10 value was obtained to be 0.20 kgy compared with 0.25 kgy at parent strain. On S. enteritidis YK-2, D10 value was obtained to be 0.14 kgy at drug-resistant strain compared with 0.16 kgy at parent strain. D10 value was also obtained to be 0.15 kgy at drug-resistant strain compared with 0.21 kgy at parent strain of St. aureus H12. Many isolates of E. coli O157:H7 or other type of E. coli from meats such as beef were resistant to penicillin G, and looked to be no relationship on radiation resistivities between drug-resistant strains and sensitive strains. On the study of radiation sensitivity on E. coli S2 at plate agars containing antibiotics, higher survival fractions were obtained at higher doses compared with normal plate agar. The reason of higher survival fractions at higher doses on plate agar containing antibiotics should be recovery of high rate of injured cells by the relay of cell division, and drug-resistant strains by mutation are hardly induced by irradiation. Key words: drug-resistant bacteria, radiation-sensitivity, radiation decontamination, food poisoning, pathogenic microorganisms Salmonella Salmonella 4 Salmonella Salmonella typhimurium
2 5 Escherichia colisalmonella enteritidis Staphylococcus aureus 6 E. coli O157:H7 E. coli S2 S. enteritidis YK-2 St. aureus H12 E. coli O157: H7 6 E. coli S2 S. enteritidis YK-2 St. aureus H unit Tryptic soy agar 7M 1.2ml 0 1.2kGy Tryptic soy agar E. coli S2 S. enteritidis YK-2 G unit E. coli S2-R S. enteritidis YK-2R Table 1 St. aureus H12 G 10 unit H12-R G 10 unit E. coli S2 D kGy S2-R D kGy S. enteritidis Fig. 1 YK-2 D kGy YK-2R 0.14kGy St. aureus H12 D kGy H12-R 0.15kGy 6 E. coli O157:H7 Table 1 Growth of drug-resistant strains on plate agars containing different concentration of antibiotics. Strain E. coli S2 S2-R S. enteritidis YK-2 YK-2R St. aureus H12 H12-R 10 unit Penicillin G 500 unit 1000 unit ; abundant growth, ; moderate growth, ; scantly growth, ; growth or not, ; not growth Chloramphenicol 10 mg/l Streptmycin 100 mg/l
3 Fig. 1 Comparative sensitivities of S. enteritidis YK-2 and YK-2R to gamma irradiation under air-equilibrium. YK-2, YK-2R E. coli Table 2 G E. coli O157:H7 G 1000 unit Tryptic soy agar 5 mg/l Tryptic soy agar E. coli S2 S2-R Fig. 2 Tryptic soy agar 0.4kGy 0.8kGy 32 Table 3 1.2kGy 16 G Table 2 Resistibility to penicillin G and radiation sensitivities (D10) on wild strains of E. coli. Isolate A4-1 A4-2 (O157 ; H7) C1 (O157 ; H7) B4 CL1-2 S2 IID959 (O157 ; H7) Growth on penicillin G 500 unit D10 value (kgy)
4 Fig. 2 Radiation sensitivities of E. coli S2 on plates of Tryptic soy agar, 5 mg/l of chloramphenicol agar and 1000 unit of penicillin G agar. Tryptic soy agar plates, 5 mg of chloramphenicol agar plates, 1000 unit of penicillin G agar plates Table 3 Growth rate of 32 isolates on antibiotics agar plates after irradiation of E. coli S2 on Tryptic soy agar plates. Antibiotic agar plate non-irrad. 0.4kGy 0.8kGy Penicillin G, 500 unit Incubation for 1 day Penicillin G, 1000 unit Incubation for 1 day Incubation for 2 days Chloramphenicol, 10mg/L Incubation for 1 day Incubation for 2 days * 11 of 16 isolates from 1.2kGy chloramphenicol agar grow at 1000 unit penicillin agar unit 5 7 E. coli E. coli O157:H7 Pseudomonas
5 aeruginosa E. coli 8 Salmonella E. coli E. coli 10 6), 9) 2 3kGy 6), 9) E. coli O157:H7 E. coli 10) 7 E. coli ) E. coli Organic Consumers Association : Tainted, drugresistant meat common Studies stir debate on antibiotic use in livestock. C. L. Hoface, et al.: Characterization of antibioticresistant bacteria in rendered animal products, Avian Dis., 45(4), (2001) J. N. Sofos et al.: Sources and extent of microbiological contamination of beef carcasses in seven United States slaughtering plants, J. Food Prot., 62(2), (1999) IASR Salmonella typhimutium 24, (1989), Harsojo O157;H (1998) Pseudomonas radiora 46(3), (1972) Y. Prachasitthisak, D. Banachi and H. Ito : Shelf life extension of chicken meat by -irradiation and microflora changes, Food Sci. Technol, Int., 2(4), (1996) Feb., (1997) Escherichia coli 30, (1995)
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