Scientific Cameras. Scientific Cameras

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1 Scientific Scientific Scientific imaging requires high-performance cameras. Thorlabs Scientific-Grade CCD are specifically designed for microscopy and other demanding applications. Our cameras are based on high quantum efficiency, low-noise CCD imagers, which make them ideal for multispectral imaging, fluorescence microscopy, and other imaging techniques. The features and options in our cameras have been chosen and optimized to benefit and enhance your research. For instance, cooled cameras are available for lowlight, long exposure applications. Choose from three industry-standard interfaces designed to ease system integration. An intuitive software package, API and SDK for developers, and third-party software support provide options for custom system control and image acquisition.

2 Solutions for Your Imaging Needs Our Feature Industry- Standard C-Mount Threading for Direct Compatibility with Most Microscopes Feature Optical & Imaging A Choice of Four High Quantum Efficiency, Low Noise Sensors: (Monochrome or Color) u 4 Megapixel (Monochrome or Color) u 8 Megapixel (Monochrome or Color) u 2 fps Fast Frame Rate, VGA Resolution (Monochrome) Removable Infrared Filter Included High Quantum Efficiency (See Specifications for Details) Low Read Noise (See Specifications for Details) System Integration Software-Selectable 2 MHz or MHz Readout Asynchronous Reset and Triggered Modes Bulb Exposure Mode Region of Interest (ROI) Mode Binning Mode Hermetically Sealed Chamber with Fanless TE-Cooling (Cooled Versions) C-Mount (1."-32) Threaded Lens Mount USB 3., Gigabit Ethernet, or Camera Link interface Robust Design with Small Form Factor Compatible with Thorlabs 6 mm Cage System Auxiliary Port and Available Cables and Accessories Software ThorCam Software GUI Third-Party Software Support Including LabVIEW, µmanager/ ImageJ, Metamorph, and MATLAB Supported by Full-Featured API/SDK Benefit Choose the Camera with the Resolution and Frame Rate Ideal for Your Application (Turn the Page to See Application Examples) Remove the Filter for NIR Applications, or Replace with Any Ø2 mm Filter to Image Wavelengths of Interest Maximizes Camera Output and Improves SNR for a Given Amount of Light at Wavelengths of Interest Improves the Threshold of Detectability Under Low Light Conditions Maximize Frame Rate for Fast Imaging or Select Slower Readout to Minimize Noise Complete Timing Control for Flexible System Integration Control the Duration and Instant of Exposure with a Single Input Pulse Select a Sub-Frame Rectangular Region for Faster Readout without Sacrificing Spatial Resolution Allows a Lower-Noise, Faster Readout of the Entire Frame at a Lower Spatial Resolution Ideal for Low Signal Levels and/or Long Exposures; No Additional Vibration During Long Exposures Integrate with Microscopes or Lenses with C-Mount Threads Industry-Standard, Robust, High-Bandwidth Interfaces Easily Integrates with Existing Equipment Integrate into a Custom Imaging System Eases System Integration and Timing for Unique Situations One Package for System Control, Acquisition, and Play Back of Images and Image Sequences Integrate Thorlabs into a Third-Party Imaging Platform of Choice Ideal for System Integrators and Developers of Custom Applications

3 Camera Overview Thorlabs scientific cameras are offered in two package styles: a compact, non-cooled package and a hermetically sealed package with a two-stage TEC. The fan-free cooler design provides optimal CCD cooling without vibration, critical for capturing long-exposure images in low-light conditions. Each camera is available with either a USB 3., Gigabit Ethernet (GigE), or a Camera Link interface. GigE is ideal when the camera must be far from the PC or when there are multiple cameras that need to be controlled by the same PC. The USB 3. and Camera Link interfaces offer higher maximum data transfer rates, which are important for certain modes of operation. Hermetically Sealed Cooled Camera Our cameras have three operating modes: streaming overlapped exposure, asynchronous edge-triggered image acquisition, and bulb mode exposure. Each mode may be operated at either 2 or MHz and can support variable binning and region-ofinterest operation. The cameras feature standard C-mount (1."-32) threading, and Thorlabs provides a full line of thread-to-thread adapters for compatibility with other thread standards, including the SM1 (1.3"-) threading used on our Ø1" Lens Tubes. The front face is also equipped with 4- tapped holes for compatibility with our 6 mm Cage System. Four 1/4"-2 tapped holes, one on each side of the housing, are compatible with our Ø1" Posts. These flexible mounting options makes Thorlabs cameras the ideal choice for integration into home-built imaging systems as well as those based on commercial microscopes. Non-Cooled Camera Software ThorCam is powerful software for 32- and 64-bit Windows 7 systems. This easy-touse graphical interface communicates with the camera to provide system control, image acquisition, and play back. Single-image capture and image sequences are supported. ThorCam Software GUI Application programming interfaces (APIs) and a software development kit (SDK) are included for the development of custom applications by OEMs and developers. Support for third-party software packages, such as LabVIEW, MATLAB, µmanager/imagej, and Metamorph, is provided.

4 Images from Selected Applications Intracellular Dynamics Fast Frame Rate can be used for Ca 2+ ratiometric studies of intracellular dynamics. Highspeed imaging is made possible by the fast frame rate of the camera as two excitation wavelengths are switched in rapid succession. Alternately, quantitative imaging data can be acquired from fluorescence emission at two distinct wavelengths. u Fast Frame Rate, VGA u ROI and Binning Modes Brightfield Microscopy A brightfield microscopy image showing Ki-67 labeled tonsil cells. Ki-67 is an antigen that only appears in the nuclei of cells undergoing division; therefore, it is an excellent marker to indicate the growth fraction of a cell population. u 4 Megapixel u 8 Megapixel Key Specification u High Resolution Ophthalmology (NIR) Retinal/fundus imaging in the NIR to view the blood vessels in the eye. u NIR Responsivity Neuroscience Fluorescence image of a rat neuron using X magnification. u TE Cooling

5 u 4 Megapixel u TE Cooling Flurorescence Microscopy Merged triple emission fluorescence microscopy image. The sample slide consists of multi-labeled bovine pulmonary artery endothelial cells (BPAEC), showing at least one example of a double nucleus. u 4 Megapixel Multispectral Imaging A series of multispectral images taken with different passband wavelengths; the final stacked color image is shown. High QE scientific cameras are especially beneficial for obtaining low light, narrowband images. This image was acquired using a Thorlabs KURIOS-WB1 Liquid Crystal Tunable Filter. u 4 Megapixel u Fast Frame Rate, VGA u ROI and Binning Modes Scanning Electron Microscopy Scanning Electron Microscope (SEM) image of a nickel sample. Electron Backscatter Diffraction (EBSD) results in Kikuchi patterns that result from the interaction between the electron beam and the sample material. Our high QE, low noise cameras make possible high speed detection and analysis of these faint line patterns against relatively high backgrounds. u NIR Responsivity Simultaneous NIR/DIC and Fluorescence One common technique to monitor complex cell dynamics uses fluorophores to identify relevant cells within a sample, while simultaneously using NIR or differential interference contrast (DIC) microscopy to probe individual cells. Here mouse kidney cells were imaged using a dichroic filter to acquire live fluorescence and DIC images with two different cameras. These images were then combined into a 2-channel composite live image with a false color applied to the fluorescence image by the ThorCam overlay plugin.

6 Specifications Higher Speed Higher Pixel Count/Larger FOV Fast Frame Rate, VGA a 1.4 Megapixel 4 Megapixel 8 Megapixel Item # Prefix 3M and 3UV 11M and 11C 7M and 7C 81M and 81C Sensor Type On Semi / Truesense KAI-3 Monochrome CCD Sony ICX28AL Monochrome and Color CCD On Semi / Truesense KAI- 7 Monochrome and Color CCD On Semi / Truesense KAI- 8 Monochrome and Color CCD Effective Number of Pixels (Horizontal x Vertical) 6 x x 248 x x 2472 Pixel Size 7.4 µm x 7.4 µm 6.4 µm x 6.4 µm 7.4 µm x 7.4 µm. µm x. µm Optical Format 1/3" (.92 mm Diagonal) 2/3" (11 mm Diagonal) 4/3" (21.4 mm Diagonal) 4/3" (22 mm Diagonal) Gigabit Ethernet Max Frame Rate 2.7 fps (at MHz Dual- 23 fps (at MHz Single- 13 fps (at MHz Dual- 8. fps (at MHz Dual- USB 3. Max Frame Rate 2.7 fps (at MHz Dual- 23 fps (at MHz Single- 2.8 fps (at MHz Quad fps (at MHz, Quad- Camera Link Max Frame Rate 2.7 fps (at MHz Dual- 23 fps (at MHz Single- 2.8 fps (at MHz Quad fps (at MHz, Quad- Quantum Efficiency (Peak) Standard Version: % at nm UV Version: % at 48 nm Monochrome: 6% at nm Color: See Plots on Next Page Monochrome: 2% at nm Color: See Plots on Next Page Monochrome: % at nm Color: See Plots on Next Page Read Noise < e- at 2 MHz <6 e- at 2 MHz < e- at 2 MHz < e- at 2 MHz Gigabit Ethernet Digital Output b Single Tap: 14 Bit Dual Tap: 12 Bit 14 Bit Single Tap: 14 Bit Dual Tap: 12 Bit Single Tap: 14 Bit Dual Tap: 12 Bit USB 3. Digital Output b 14 Bit 14 Bit 14 Bit 14 Bit Camera Link Digital Output b 14 Bit 14 Bit 14 Bit 14 Bit Vertical Hardware Binning c Values from 1 to 24 Values from 1 to 24 Values from 1 to Values from 1 to Horizontal Software Binning Values from 1 to 24 Values from 1 to 24 Values from 1 to Values from 1 to Region of Interest 1 x 1 Pixel to Effective Number of Pixels, Rectangular Available Cooled Version None -2 C at 2 C Ambient Temperature - C at 2 C Ambient Temperature - C at 2 C Ambient Temperature Host PC Interfaces Available Gigabit Ethernet, USB 3., and Camera Link Lens Mount C-Mount (1."-32) a. Cooled Option Not Offered. b. Camera Link and USB 3. Versions support single-, dual-, or quad-tap operation. GigE versions support single- or dual-tap operation. c. Camera frame rate is impacted by the vertical hardware binning parameter.

7 Quantum Efficiency Plots 6 6 Fast Frame Rate Standard UV Enhanced Megapixel Standard Mode NIR Enhanced (Boost) Mode Relative Sensitivity Megapixel Color Red Green Blue Megapixel Megapixel Color Red Green Blue Megapixel Megapixel Color Red Green Blue

8 Housing Dimensions 1.81" (46 mm) 3.2" (82.6 mm) 2.6" (67.2 mm) 3.9" (99.1 mm).3" (8 mm) 2.36" (6 mm) 1.17" (29.7 mm) 2.36" (6 mm) 1.63" (41.3 mm) 3.2" (82.6 mm) 1.9" (49. mm) 3.9" (99.1 mm) C-Mount (1."-32) (Back Focus Adjustable) 2.36" (6 mm) C-Mount (1."-32) (Back Focus Adjustable) 2.36" (6 mm) 1/4"-2 Tapped Hole (4 Places) Non-Cooled Camera Custom 4- Tap for 6 mm Cage System (4 Places) A scientific camera can be customized by using one of our existing standard cameras as a starting point, or it can be built to the exact needs of an application. Our engineering team simplifies this process by using the two-step process below. Small changes made to our existing designs can be done efficiently. If you have special requirements, a custom application, or general questions about our capabilities, please contact us at ScientificImaging@thorlabs.com. 1/4"-2 Tapped Hole (4 Places) Hermetically Sealed Cooled Camera Board-Level Photograph of the Sensor and Electronics in One of Our Scientific 4- Tap for 6 mm Cage System (4 Places) Step 1: Analyze Your Custom Requirements Considerations u Sensitivity u Wavelength System Requirements u Operating Environment u Space Constraints Application Space u Compliance Issues u Future Developments u Resolution u Speed u Interfaces u Software u Logistics Popular Fluorophore Emission Wavelengths Overlaid with the Quantum Efficiency Curves for our 1.4 Megapixel Step 2: Configure a Solution Imager Options u UV, Visible, or NIR Spectrum, 4 Megapixel, 8 Megapixel, or Fast Frame Rate CCD u CMOS or scmos I/O Options u Camera Link u Gigabit Ethernet u USB 3. Camera Body Options u Standard Non-Cooled u Hermetically Sealed with Two-Stage TEC u Private Labeling Electronics Modifications Optics Mounting Options u C-Mount Threading is Standard Software u u Initial Evaluation Using ThorCam GUI for Algorithm Development Using Popular Third-Party Support Such as MATLAB, µmanager/imagej, LabVIEW, and Metamorph u API / SDK Provided for Software Developers and OEMs Supply Chain u Kanban Stocking Agreements 6 Sparta Avenue Newton, New Jersey 786 Sales: Fax: USA Thorlabs Imaging Systems Phone: Thorlabs Scientific Imaging (TSI) Phone: Thorlabs Quantum Electronics (TQE) Phone: UK Phone: +44 () GERMANY Phone: +49 () FRANCE Phone : +33 () China Phone: +86 () SWEDEN Phone: Polish Direct Line: JAPAN Phone: BRAZIL Phone: + (16)