Current situation. Professional experience. Cursus

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1 Anne des Rieux PhD in Pharmaceutical Sciences Chercheur Qualifié FNRS Université Catholique de Louvain Unité de Pharmacie Galénique Av. E. Mounier, 73, UCL 73.20, 1200 Bruxelles Tél.: Fax : Current situation : FNRS Research Associate, Chercheur Qualifié in the Pharmaceutics and Drug delivery unit (Prof. V. Preat), Catholic University of Louvain (Brussels, Belgium). Implementation of a new research activity dealing with tissue engineering in general, and spinal cord regeneration in particular. Professional experience Academia: : Research assistant position: Chargée de recherche (mandat FNRS) in the Pharmaceutics and Drug delivery unit (Prof. V. Preat), Catholic University of Louvain (Brussels, Belgium). Implementation of a new research activity dealing with tissue engineering in general, and spinal cord regeneration in particular : Post-Doctoral assignment: Spinal cord regeneration project. The Shea Lab for Cellular and Tissue Engineering, Northwestern University, Chicago. BAEF (Belgian-American Educational Foundation) post-doctoral fellowship : Research assistant in the Pharmaceutical Technology Unit, (PhD student in pharmaceutical sciences), Catholic University of Louvain (Brussels, Belgium). 2003: Uppsala University (Sweden), Department of Pharmacy, Prof. Artursson lab ; Invited scientist for a 6 months stay in the context of FIRST Europe fellowship. Industry: : BAXTER R&D Europe, Corporate Research, Exploratory group (Nivelles, Belgium) ; Research engineer Research activities within a corporate research group, aiming to develop a technologic platform, based on fibrin used as a bio-engineering material (bone regeneration). Cursus : PhD student in pharmaceutical sciences, Catholic University of Louvain (Brussels, Belgium). Doctoral thesis presented in September, 15th An improved in vitro model of human M cells as a useful tool to study nanoparticle transport across the intestinal epithelium" : Post-graduate level in pharmaceutical sciences, Catholic University of Louvain (Brussels, Belgium), magna cum laude

2 : Graduate level in biomedical engineering, Institut Universitaire Professionnalisé (IUP), Pharmaceutical Science faculty of Montpellier (France) : Undergraduate level in Applied Biology, Institut Universitaire Technologique (IUT), University of Paul Sabatier (Toulouse, France). Publications 21 papers published in international journals For detail, see appendix. Personal information Born in Le Havre (France), on November the 28 th 1976, French, Married, two children

3 APPENDIX Articles : A. des Rieux, P. De Berdt, E. Ansorena, B. Ucakar, D. Jacobs, O. Schakman, E. Audouard, C. Bouzin, D. Auhl, T. Simón-Yarza, O. Feron, M. J. Blanco-Prieto, P. Carmeliet, C. Bailly, F. Clotman, V. Préat VEGF-loaded injectable hydrogel enhances plasticity in the injured spinal cord, Journal of Biomedical Materials Research: Part A, 2013, accepted E. Ansorena, P. De Berdt, B. Ucakar, T. Simón-Yarza, D. Jacobs, O. Schakman, A.Jankovski, R. Deumens, M. J. Blanco-Prieto, V. Préat, A. des Rieux Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury, International Journal of Pharmaceutics, 2013, accepted. K. Kumar Chereddy, R. Coco, P. B. Memvanga, B. Ucakar, A. des Rieux, G. Vandermeulen, V.Préat Combined effect of PLGA and Curcumin on Wound Healing Activity, 2013, in press des Rieux A., Pourcelle V., Cani P. D., Marchand-Brynaert J., Préat V. Targeted nanoparticles with novel non-peptidic ligands for oral delivery. Advanced drug delivery reviews, 2013, 65, Beloqui A., Solinís M.A., Gascón A.R., del Pozo-Rodrígue A., des Rieux A., Préat V. Mechanism of transport of saquinavir-loaded nanostructured lipid carriers across the intestinal barrier, Journal of controlled release, 2013,166(2), Vanacker J., Luyckx V., Dolmans M.M, des Rieux A., Jaeger J., Van Langendonckt A., Donnez J., Amorim C.A. Transplantation of an alginate-matrigel matrix containing isolated ovarian cells:first step in developing a biodegradable scaffold to transplant isolated preantral follicles and ovarian cells. Biomaterials, 2012 ; 33(26), (2012). De Laporte L., des Rieux A., Tuinstra H.M., Zelivyanskaya M.L., De Clerck N.M., Postnov A.A., Préat V., Shea L.D. VEGF and FGF-2 delivery from spinal cord bridges to enhance angiogenesis following injury, Journal of Biomedical Materials Research: Part A, 2011, 98(3): des Rieux A., Ucakar B., Kaishusha Mupendwa B.P., Colau D., Feron O., Carmeliet P., Préat V. 3D systems delivering VEGF to promote angiogenesis for tissue engineering, Journal of Controlled Release, 2011, 150(3): Plapied L., Nicolas Duhem N., Anne des Rieux A., Véronique Préat V. Fate of polymeric nanocarriers for oral drug delivery, Current Opinion in Colloid & Interface Science, 2011, 16(3), Fievez V., Plapied L., Plaideau C., Legendre D., des Rieux A., Pourcelle V., Freichels H., Jérôme C., Marchand J., Préat V., Schneider Y.J. In vitro identification of targeting ligands of human M cells by phage display. International Journal of Pharmaceutics, 2010, 392, Plapied L., Vandermeulen G., Vroman B., des Rieux A. Bioadhesive nanoparticles of fungal chitosan for oral DNA delivery International Journal of Pharmaceutics, 2010, 398, Staes E., Absil P.A., Lins L., Brasseur R., Deleu M., Lecouturier N., Fievez V., des Rieux A., Mingeot-Leclercq M.P., Raussens V., Préat V. Acylated and unacylated ghrelin binding to membranes and to ghrelin receptor: towards a better understanding of the underlying mechanisms BBA-Biomembranes, 2010, 1798, des Rieux A., Shikanov A. Shea L.D. Fibrin hydrogels for non-viral vector delivery in vitro Journal of Controlled Release, 2009, 136, Fievez V., Plapied L., des Rieux A., Pourcelle V., Freichels H., Wascotte V., Vanderhaeghen M.-L., Jérôme C., Vanderplasschen A., Marchand-Brynaert J., Schneider Y.J., Préat V. Targeting nanoparticles to M cells with non-peptidic ligands for oral vaccination European Journal of Pharmaceutics and Biopharmaceutics., 2009, 73, Impact Factor ,

4 Rives C., des Rieux A., Zelivyanskaya M., Stock S., Lowe W., Shea L.D. Layered PLG scaffolds for in vivo plasmid delivery Biomaterials, 2009, 30, Slutter B., Plapied L., Fievez V., Alonso Sande M., des Rieux A., Schneider Y.J., Van Riet E., Jiskoot W., Préat V. Mechanistic study of the adjuvant effect of biodegradable nanoparticles in mucosal vaccination Journal of Controlled Release, 2009, 138, des Rieux A., Fievez V., Momtaz M., Detrembleur C., Alonso-Sande M., Van Gelder J., Cauvin A., Schneider Y.J., Préat V. Helodermin-loaded nanoparticles: Characterization and transport across an in vitro model of the follicle-associated epithelium Journal of Controlled Release, 2007, 118, des Rieux A., Fievez V., Théate I., Mast J., Préat V., Schneider Y.J. An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells European Journal of Pharmaceutical Sciences, 30, 2007, Garinot M., Fievez V., Pourcelle V., Stoffelbach F., des Rieux A., Plapied L., Theate I., Freichels H., Jérôme C., Marchand Brynaert J., Schneider Y.J., Préat V. PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination. Journal of Controlled Release, 2007, 120, Mathot F., des Rieux A., Ariën A., Schneider Y.J., Brewster M., Préat V. Transport mechanisms of mmepeg750p(cl-co-tmc) polymeric micelles across the intestinal barrier. Journal of Controlled Release, 2007, 124, Cover story: - From Article #1 above: Park K., Non viral vector delivery in vitro using fibrin hydrogels, Journal of Controlled Release, 136 (2): 87 (2009) Book chapter: Riva, R., Ragelle, H., des Rieux, A., Duhem, N., Jérôme, C., Préat, V. Chitosan and chitosan derivatives in drug delivery and tissue engineering. In Jayakumar, & Rangasamy, Chitosan for Biomaterials II (pp ). Springer. Oral communications: - A. des Rieux, E. Ansorena, D. Jacobs, A. Jankovski, B. Ucakar, P. Carmeliet, C. Raftopoulos, V. Préat, Injectable Hydrogel-based Formulations for Spinal Cord Regeneration, 5th World Congress on Preventive & Regenerative Medicine, Hannover, 5-7 October 2010, Germany - Invited speaker, Transport of nanoparticles to M cells for oral vaccination, A. des Rieux, V.Fievez, L.Plapied, V. Préat, Y-J. Schneider ESF-EMBO Symposium, B Cells and Protection: Back to basics, April Hotel Eden Roc, Sant Feliu de Guixols, Spain - A. des Rieux, V. Fievez, I. Théate, V. Préat, Y-J. Schneider Characterization of an in vitro model of the human intestinal FAE to study the mechanisms of nanoparticle transport by M cells, 33 rd Annual meeting of the Controlled Release Society, July 22-26, Vienna (Austria) Influence of M cells in the transport of therapeutic peptides across the intestinal mucosa, 12th forum of Pharmaceutical Sciences, May 2004, Belgium Study of Nanoparticle Transport Properties Through an Optimized Human Intestinal Follicle Associated Epithelium (FAE) Model, XVIII meeting of GTRV 2003, Lille (France) - F. Mathot, L. Van Beijsterveldt, A. des Rieux, A. Schanck, F. Van Bambeke, A. Ariën, M. Brewster, V. Préat Intestinal transport mechanisms and biodistribution of novel polymeric micelles after oral deliver, 33 rd Annual meeting of the Controlled Release Society, July , Vienna (Austria), Capsugel/Pfizer special session Awarder.

5 Posters: - E. Ansorena, P. De Berdt, B. Ucakar, T. Simón-Yarza, D. Jacobs, O. Schakman, A.Jankovski, R. Deumens, M. J. Blanco-Prieto, V. Préat, A. des Rieux GDNF delivery from an alginate-based injectable hydrogel supports functional recovery of rats whose spinal cord has been hemisected, Biomaterials and Tissue Engineering Gordon Research Conference meeting, 28/07 to 02/08/ A. des Rieux, A. Shikanov, L. D. Shea Fibrin hydrogels as non-viral vector delivery systems for tissue engineering applications, 35th annual meeting and Exposition of the Controlled Release Society, New York, juillet A. des Rieux, L. De Laporte, H. Tuinstra, C. Rives, M. Zelivyanskaya, C. Ruiz de Almodovar, P. Carmeliet, L. Shea VEGF-releasing scaffolds for spinal cord regeneration application, 35th annual meeting and Exposition of the Controlled Release Society, New York, juillet L. Plapied, V. Fievez, A. des Rieux, B. Vroman, V. Maquet, YJ Schneider, V. Préat Bioadhesive nanoparticles of novel GMP vegetal chitosan for oral vaccination with protein or DNA, 35th annual meeting and Exposition of the Controlled Release Society, New York, juillet V. Fievez, L. Plapied, M. Garinot, V. Pourcelle, F. Stoffelbach, A. des Rieux, C. Jérôme, J. Marchand, YJ. Schneider, V. Préat Nanoparticulate systems targeted to M cells with new non-peptidic analogs, 35th annual meeting and Exposition of the Controlled Release Society, New York, juillet A. des Rieux, L. De Laporte, H. Tuinstra, C. Rives, M. Zelivyanskaya, V. Préat, P. Carmeliet, L. Shea Spinal cord regeneration : influence of VEGF on blood vessel and nerve outgrowth, XXIIème journés scientifiques du G.T.R.V, Strasbourg, décembre A. des Rieux, V. Fievez, M. Momtaz, C. Detrembleur, Maria Alonso-Sande, Y-J. Schneider, V. Préat Influence of M cells on Helodermin Oral Delivery, 33 rd Annual meeting of the Controlled Release Society, July , Vienna (Austria). - M. Alonso-Sande, A. des Rieux, Y-J Schneider, C. Remuñán-López, M. J. Alonso, V. Préat Uptake studies of chitosan and chitosan-glucomannan nanoparticles in human intestinal FAE model, 33 rd Annual meeting of the Controlled Release Society, July , Vienna (Austria). - M. Garinot, V. Fievez, V. Pourcelle, F. Stoffelbach, A. des Rieux, I. Théate, C. Jerôme, J. Marchand, Y-J. Schneider, V. Préat Nanoparticulate systems targeted to M cells for oral vaccination, 33 rd Annual meeting of the Controlled Release Society, July , Vienna (Austria). - A. des Rieux, V. Fievez, M. Momtaz, C. Detrembleur, Y-J. Schneider, V. Préat Nanoencapsulation of helodermin and its transport by M cells using an in vitro model of the human Follicle Associated Epithelium, PharmSciFair 2005, Nice, (France) - A. des Rieux, V. Fievez, I. Théate, V. Préat, Y.-J. Schneider An in vitro follicle associated epithelium (FAE) model as a new application of the Caco-2 cell model, 19th meeting of ESACT 2005, Harrogate (UK). Influence of nanoparticle properties on their transport through an optimized Human intestine Follicle Associated Epithelium (FAE) model, EUFEPS 2004, Brussels (Belgium) - E.G.E. Ragnarsson, Anne des Rieux, E. Gullberg., Artursson P. Transport properties of polystyrene latex particles using an in vitro M-cell like mode, 19th JSSX Annual Meeting (Kanazawa), (best poster price). - Y. Delmotte, A. des Rieux, V. Pector, and G. Krack Fibrin Honeycomb as a bone substitute use in orthopaedic surgery, Baxter Global R&D conference, 2001, Chicago (IL) - 2 Baxter Invention Records (Confidential internal documents).

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