Phosphorus Analyzer Procedure Last updated: A. Introduction
|
|
- Jemima Marianna Reeves
- 8 years ago
- Views:
Transcription
1 Phosphorus Analyzer Procedure Last updated: A. Introduction The phosphorus analyzer can be used to measure soluble reactive phosphorus (SRP) aka orthophosphate (PO 3-4 ) only. Additionally, this result when subtracted from the total phosphorus (TP) from the ICP, can calculate total dissolved phosphorus (i.e. soluble plus insoluble). This is a colorimetric technique where the ammonium molydbdate reacts with the sample to form a blue phosphomolybdenum. The wavelength can then be read by a spectrophotometer inside the machine. B. Set Up Lab Equipment/Supplies P Analyzer Vials KH 2 PO4 salt Glassware Deionized water Pipette tips for making 5 N sulfuric acid (H 2 SO 4 ) Ammonium molybdate salt Ascorbic acid salt (vitamin C) Potassium antimonyl tartate salt Dowfax 2A1 surfactant (lubricant) Gloves Lab coat and safety glasses (for handling acid) C. Procedure Overview 1. Prepare samples for analysis. 2. Empty waste bottle. Refill other bottles. 3. Turn on machine and start pumps. 4. Prepare sequence table with standards, method blanks, and samples. 5. Warm up analyzer. 6. Prepare reagent solution. 7. Run sequence. 8. End sequence and export data to personal folder. 9. Run cleaning solutions through analyzer. 10. Shut off pumps and turn off machines. Soil & Water Lab, Phosphorus Analyzer Procedure Page 1
2 D. Making Stock Solution and Serial Dilutions Corresponds to Step 11 in Section F. Running the Machine. 1. Add g of KH 2 PO4 salt into 100 ml of DI water to make 1000 ppm stock solution. Top off with DI water and mix well. 2. Dilute accordingly using a 100 ml flask: Concentration Amount to Add (ml) Using this Concentration 0 ppm 100 DI water ppm ppm ppm ppm 0.5* ppm * The 0.5 ppm concentration is used as a check standard throughout the run so make sure you have enough volume to fill a few extra vials. 3. Top off the flasks with DI water and mix well. 4. Pour into storage-appropriate containers and label with date. Refrigerate until use E. Making the Reagent Solution Corresponds to Step in Section F. Running the Machine. Note! Usually making 100 ml is enough for up to 50 samples. The fully mixed regent solution only lasts about 4 hours so be sure to watch the timing. 1. Prepare to mix the reagent solution under the hood by retrieving 5 reagents. 2. 5N sulfuric acid is kept in the acid cabinet below the hood. Use gloves when handling. If needed make 5N sulfuric acid. Stock is typically 36N. Pour this in the hood and wear protective glasses and a lab coat. Be careful when you rinse glassware after use. All rinse water should be neutralized before dumping it down the drain. To make 5N, mix 140 ml into ~800 ml of DI water and fill to 1 L. 3. Potassium antimonyl tartate is kept refrigerated. To make add 0.3 g potassium antimonyl tartate to 100 ml DI water. Mix well. 4. Ammounium molybdate is kept refrigerated. To make add 4 g ammounium molybdate to 100 ml of DI water. Mix well. 5. Ascorbic acid is kept refrigerated, but for best results it should be made the same day. It will break down in about 1 week. To make, add 1.8g ascorbic acid salt from the fridge and add 100 ml of DI water. Mix well. Final produce is 0.01 M ascorbic acid. Return the salt to the fridge. 6. Dowfax lubricant is located above the bench top across from the analyzer. 7. When ready, mix the reagent in the following order (order is important!): Soil & Water Lab, Phosphorus Analyzer Procedure Page 2
3 Reagent ml to add Full Run (approx. 100 samples) Small Run (approx.. 50 samples) 1. 5 N sulfuric acid Potassium antimonyl tartate Ammonium molybdate Ascorbic acid Dowfax lubricant 0.5 (15 drops) 0.25 (8 drops) Final color reagent volume, ml Swirl the color reagent together in a beaker. It should be slightly yellow but not blue. Blue indicates contamination. 9. Store all extra liquids separately in the fridge. F. Running the Machine 1. Prepare samples for analysis by pouring >5mL of sample into P analyzer tubes. See Section G for recommendations on filtering and preserving samples before analysis. 2. Empty the waste bottle next to the machine into the acid waste jar found in the sink. Neutralize with baking soda. Add baking soda until the solution stops reacting when you add it. You can also test the ph to make sure that it s around 7 before dumping it down the sink. Sometimes the baking soda will react and the foam will overflow the acid waste jar. To prevent harm to the sink and pipes, be sure to run the tap water while dumping it down the sink and also while adding the baking soda. 3. Refill the DI and DI+dowfax bottles to the top. To make the DI+dowfax solution, mix 2 ml (60 drops) of dowfax into 1 L of water. 4. Remove the clear plastic dust cover from the autosampler. 5. Turn the machine on by flipping the two switches on the right, back of the instrument (top and bottom). One works the auto-sampler and the other works the main machine. The auto-sampler arm should move up and down when you do this. 6. Turn the computer on and enter the user (administrator) and password (syd-8-ney). 7. Click on the P Analyzer Winflow icon to open the program. 8. Turn on the pumps. Pumps > Turn On> All Pumps. 9. Engage the platens (black domino shaped pieces). It might take a firm push to get them into place. Be sure not to spin the silver knobs as they control the flow of the liquid through the tubes. 10. Click the sequence button in the upper left hand corner (looks like a spreadsheet) to make a sequence. Save the file in your personal folder. See sample sequence in Section H.. Standards 1 through 6 should go in the first 6 slots. Then the controls follow; the standard check is the 0.5 ppm standard. If you have a lot of samples then slots 7-10 should be filled with the standard check (0.5 ppm) but if you only have ~40 samples then just need to fill slots 7 and 8. Samples should start at slot number 11. If you wish to intersperse with check standards and also repeat all the standards at the end this is an option. It is also a good idea to program in a blank (with repetitions) between each sample to avoid carryover between samples. It is recommended to put a known check standard (mid-range concentration) after every 10 samples so you can calculate the instrument s drift factor. 11. Load the vials full of filtered sample into the auto-sampler tray Soil & Water Lab, Phosphorus Analyzer Procedure Page 3
4 12. Click the schedule button on the left side of the screen (looks like a series of sin waves) to open the sequence that you just saved. If it asks for a operator ID, just type the name of the folder that you saved the sequence to (e.g. Sheila). 13. Press the play button and wait for the line on the plot to stabilize. This takes approximately 40 minutes. The machine is set up to run for 1 hour before advancing automatically to the first line in your sequence table. 14. While the machine is stabilizing, mix your reagent. See section E. Making Reagent Solution. Rinse out the reagent bottle (and tube if necessary) with DI water and fill it with the newly mixed reagent. 15. Once the signal looks relatively stable or during the last minutes of the recommended 1 hour take the tube that s attached to the *R slot and move it from the DI+dowfax bottle the brown reagent bottle. 16. Wait until the noise of signal is no more than 200 microam (about 30 minutes). Right click on the plot and select Full Scale to zoom out. To zoom in, right click and drag a rectangle around the plot area you wish to zoom to. 17. When the signal looks stable press the fast forward button to start the run. 18. Watch the peak of the 10 ppm sample as it goes through. This is the SYNC line of the sample table. The peak should be symmetrical with one peak that top at 260,000 to 280,000 microam.. This will take about two minutes to see on the plot. If the peak doesn t appear or reach the suggested range then there is something wrong with the patens, your reagent solution, or another part of the machine. If something is wrong and you would like to stop the machine, press the stop button, fix the problem, press the rewind button, run will not be saved, and then restart by pressing play and then the fast forward button. If peak is good then continue to run the sequence. 19. Watch the calibration curve R squared value. It should be as close to 1 as possible, ideally reaching at least If it s not then stop the run, remake your standards, and start the run again. If it s fine then keep the run going. 20. During the run periodically check that the DI cup next to the autosampler is filled above the bottom of the probe. 21. When the run is finished the results file will save as a.rls. You can find it in the same folder that you saved your sequence in. Export the results by selecting View > Reports. A window with results should pop up. Save these to your folder as a.txt file by selecting File > Export Report. Type in the file name making sure to erase the asterisk that is automatically placed before the extension. 22. Now clean the machine. First switch over the reagent tube back to the DI+dofax bottle and let the system flush out 5 minutes to flush out any remaining samples and acid. 23. Run the cleaning solutions through the machine for all sample types and all runs. It is especially important to clean the machine when you know you have dirty samples (i.e manure runoff, etc.). Cleaning the machine helps us keep the result for our blanks at ~0.02 ppm (+/ ppm). After flushing out the reagent in step 22, unscrew the tube on the R* port (DI+dowfax bottle) and connect it to the 1M NaOH solution for 20 minutes. Reconnect to the R* port for 5 minutes to flush out the NaOH and then connect to the 5N H 2 SO 4 solution for 20 minutes. Finally, reconnect to the R* port in the DI+dowfax bottle and run for 15 minutes so the whole system has a chance to be flushed out. 24. Turn off all the pumps (Pumps > Pumps Off > All Pumps) and shut down the computer. Be sure to switch off the auto-sampler and the sensor as well. Soil & Water Lab, Phosphorus Analyzer Procedure Page 4
5 G. Other Notes and Recommendations Filtering Samples All samples should be filtered with 0.45 um membrane filter before using the P Analyzer. Filters may contain P; therefore if low level accuracy is needed, filters must be pre-washed before P work. Standard Methods (Method 4500) give a procedure for this. Basically, using an empty volumetric flask to catch the flow through water, you should filter a known volume of DI water through the filter before filtering your sample. Keep the filter paper but replace the flask with the centrifuge tube used to store your filtered sample and filter your sample as you would normally. It is best to also run samples to compare unfiltered DI water with filtered DI water during your run (replicates of ~3). Preserving Samples Refrigerate samples immediately and analyze them within 48 hours for best results. Freezing samples can cause ions to precipitate out. This precipitate may be difficult to see but is detrimental to the machine and can alter your results. Shree recommends not freezing samples but instead adding approximately 100 ul of concentrated H 2 SO 4 (or enough so ph = 2) into each full 50 ml centrifuge tube immediately after filtering your samples. If you have already frozen your samples, please re-filter the thawed sample before running them through the P Analyzer. Use a 0.45 um filter. Diluting Samples If you expect your samples to be greater than 0.5ppm, it is recommended that you dilute your samples. For example a common dilution is 1mL sample : 9mL DI water. Unknown Samples For samples where the concentration is unknown and potentially greater than 0.5ppm, add additional blank reps in between samples. This is done by changing the 1 in the R column of the blank line to 2 or 3. Soil & Water Lab, Phosphorus Analyzer Procedure Page 5
6 H. Example Sequence Table Cup refers to the position on the autosampler. Name is your sample ID. R refers to the reps or number of times the machine will sample from the cup before moving on to the next line. Dil and Wt are not utilized in our set up. Cup Name Type R Dil Wt Vial Comment 6 Sync SYNC Carryover CO Baseline RB Cal 0.00 ppm C Cal ppm C Cal ppm C Cal 0.50 ppm C Cal 5.00 ppm C Cal ppm C Blank BLNK ICV W 0.5 ppm ICV Blank BLNK Read Baseline RB DI U Sample #1 U Sample #2 U After 10 samples, the following lines should be added after a blank is run. Use the Comments column to keep track of the number of times a cup is drawn from. The maximum is five times per cup. Cup Name Type R Dil Wt Vial Comment 7 CCV W 0.5 ppm CCV Blank BLNK Read Baseline RB Soil & Water Lab, Phosphorus Analyzer Procedure Page 6
7 The end of the sequence should have the following lines. Cup Name Type R Dil Wt Vial Comment 1 0 ppm check U ppm check U ppm check U ppm check U Blank BLNK ppm check U Blank BLNK ppm check U Blank BLNK X CCV W 0.5 ppm CCV X.X 0 Blank BLNK Read Baseline RB I. Tubing L refers to the left side of the pump and R refers to the right side of it Tube Color of the Top end Bottom end spot tubing L1 Green Waste bottle TO WASTE port L3 Red Waste bottle Top port of the clear bubbler L4 Red CARRIER IN port DI only bottle R1 Orange Reagent port on DI Dowfax bottle or Reagent bottle Port no. 3 R4 black DI Dowfax bottle Port no. 9 J. Instrument Model: O.I. Analytical 3000 K. Credits: Sheila Saia; Bahareh Hassanpour Guilvaiee; Chelsea Morris Soil & Water Lab, Phosphorus Analyzer Procedure Page 7
Determination of the Mass Percentage of Copper in a Penny. Introduction
Determination of the Mass Percentage of Copper in a Penny Introduction This experiment will cost you one penny ($0.01). The penny must be minted after 1983. Any penny will do; for best results the penny
More informationAppendix C. Vernier Tutorial
C-1. Vernier Tutorial Introduction: In this lab course, you will collect, analyze and interpret data. The purpose of this tutorial is to teach you how to use the Vernier System to collect and transfer
More informationDetermination of Citric Acid in Powdered Drink Mixes
Determination of Citric Acid in Powdered Drink Mixes Citric acid and its salts (sodium citrate and potassium citrate) are found in many foods, drinks, pharmaceuticals, shampoos, and cosmetics. The tartness
More informationThe Determination of an Equilibrium Constant
The Determination of an Equilibrium Constant Computer 10 Chemical reactions occur to reach a state of equilibrium. The equilibrium state can be characterized by quantitatively defining its equilibrium
More informationPhosphates (ortho- and total)
INTRODUCTION Phosphates (ortho- and total) Phosphorus is an essential nutrient for all aquatic plants and algae. Only a very small amount is needed, however, so an excess of phosphorus can easily occur.
More informationStandard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method)
Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method) Grace Analytical Lab 536 South Clark Street 10th Floor Chicago, IL 60605 April 15, 1994 Revision 2 Standard Operating Procedure
More informationDetermining the Quantity of Iron in a Vitamin Tablet. Evaluation copy
Determining the Quantity of Iron in a Vitamin Tablet Computer 34 As biochemical research becomes more sophisticated, we are learning more about the role of metallic elements in the human body. For example,
More informationPhosphorus, colorimetry, phosphomolybdate, automated-segmented flow
Phosphorus, colorimetry, phosphomolybdate, automated-segmented flow Parameter and code: Phosphorus, total-in-bottom-material, dry weight, I-6600-88 (mg/kg as P): 00668 1. Application This method is used
More informationESS Method 130.1: General Auto Analyzer Procedures
ESS Method 130.1: General Auto Analyzer Procedures Environmental Sciences Section Inorganic Chemistry Unit Wisconsin State Lab of Hygiene 465 Henry Mall Madison, WI 53706 Revised October 1992 ESS Method
More informationSPIKE RECOVERY AND DETERMINING A METHOD DETECTION LIMIT Pamela Doolittle, University of Wisconsin Madison, pssemrad@wisc.edu 2014
SPIKE RECOVERY AND DETERMINING A METHOD DETECTION LIMIT Pamela Doolittle, University of Wisconsin Madison, pssemrad@wisc.edu 2014 This experiment explores quality assurance practices which are commonly
More informationCoating and Extraction of Honeycomb Denuders
QSM Approval: Coating and Extraction of Honeycomb Denuders 1 INTRODUCTION 1.1 The following procedures are used for the coating of honeycomb denuders with citric acid and sodium carbonate solutions, and
More informationHow to operate the BD FACSCanto flow cytometer
How to operate the BD FACSCanto flow cytometer Preface Dear colleague, the BD FACSCanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells.
More informationPhosphorus, Reactive (Orthophosphate)
Phosphorus, Reactive (Orthophosphate) DOC316.53.01113 Amino Acid Method 1 Method 8178 0.23 to 30.00 mg/l PO 4 Reagent Solution Scope and application: For water, wastewater and seawater. 1 Adapted from
More informationChem 1B Saddleback College Dr. White 1. Experiment 8 Titration Curve for a Monoprotic Acid
Chem 1B Saddleback College Dr. White 1 Experiment 8 Titration Curve for a Monoprotic Acid Objectives To learn the difference between titration curves involving a strong acid with a strong base and a weak
More informationEXPERIMENT 5. Molecular Absorption Spectroscopy: Determination of Iron With 1,10-Phenanthroline
EXPERIMENT 5 Molecular Absorption Spectroscopy: Determination of Iron With 1,10-Phenanthroline UNKNOWN Submit a clean, labeled 100-mL volumetric flask to the instructor so that your unknown iron solution
More informationSolubility Product Constants
Solubility Product Constants PURPOSE To measure the solubility product constant (K sp ) of copper (II) iodate, Cu(IO 3 ) 2. GOALS 1 To measure the molar solubility of a sparingly soluble salt in water.
More information(1) Hydrochloric acid reacts with sodium hypochlorite to form hypochlorous acid: NaOCl(aq) + HCl(aq) HOCl(aq) + NaCl(aq) hypochlorous acid
The Determination of Hypochlorite in Bleach Reading assignment: Chang, Chemistry 10 th edition, pages 156-159. We will study an example of a redox titration in order to determine the concentration of sodium
More informationCOMMON LABORATORY APPARATUS
COMMON LABORATORY APPARATUS Beakers are useful as a reaction container or to hold liquid or solid samples. They are also used to catch liquids from titrations and filtrates from filtering operations. Bunsen
More informationAnalysis of Vitamin C Using Iodine. Introduction
Analysis of Vitamin C Using Iodine Introduction Vitamin C (ascorbic acid) is oxidized to dehydroascorbic acid using a mild oxidizing agent such as iodine. The oxidation is a two- electron process, following
More informationUse of Micropipettes
Use of Micropipettes Prior to lab you should understand: The function of micropipettes in the laboratory Basic parts of micropipette What volumes are measured with P, P and P1 micopipettors How to read
More informationTo determine the equivalence points of two titrations from plots of ph versus ml of titrant added.
Titration Curves PURPOSE To determine the equivalence points of two titrations from plots of ph versus ml of titrant added. GOALS 1 To gain experience performing acid-base titrations with a ph meter. 2
More informationLuminol Test PROCESS SKILLS SCIENCE TOPICS VOCABULARY
EXPERIMENT: LUMINOL TEST Luminol Test Visitors mix a solution of luminol with fake blood (hydrogen peroxide) to produce a reaction that gives off blue light. OBJECTIVES: Visitors learn that some chemical
More informationCHM 130LL: ph, Buffers, and Indicators
CHM 130LL: ph, Buffers, and Indicators Many substances can be classified as acidic or basic. Acidic substances contain hydrogen ions, H +, while basic substances contain hydroxide ions, OH. The relative
More informationDetermination of the Amount of Acid Neutralized by an Antacid Tablet Using Back Titration
Determination of the Amount of Acid Neutralized by an Antacid Tablet Using Back Titration GOAL AND OVERVIEW Antacids are bases that react stoichiometrically with acid. The number of moles of acid that
More informationCadmium Reduction Method Method 8039 0.3 to 30.0 mg/l NO 3 N (HR) Powder Pillows or AccuVac Ampuls
Nitrate DOC316.53.01066 Cadmium Reduction Method Method 8039 0.3 to 30.0 mg/l NO 3 N (HR) Powder Pillows or AccuVac Ampuls Scope and application: For water, wastewater and seawater. Test preparation Instrument-specific
More informationAcid Base Titrations
Acid Base Titrations Introduction A common question chemists have to answer is how much of something is present in a sample or a product. If the product contains an acid or base, this question is usually
More informationOBJECTIVES: Visitors learn what an antioxidant is and how it behaves. They also learn how to test for the presence of vitamin C..
Vitamin C Visitors use iodine to compare the reactivity of two starch solutions one with vitamin C added, one without vitamin C. OBJECTIVES: Visitors learn what an antioxidant is and how it behaves. They
More informationSTANDARDIZATION OF A SODIUM HYDROXIDE SOLUTION EXPERIMENT 14
STANDARDIZATION OF A SODIUM HYDROXIDE SOLUTION EXPERIMENT 14 OBJECTIVE The objective of this experiment will be the standardization of sodium hydroxide using potassium hydrogen phthalate by the titration
More informationWaters Corporation. Waters 2690/5 USER & TROUBLESHOOTING GUIDE
Waters Corporation Waters 2690/5 USER & TROUBLESHOOTING GUIDE Contents 2690/5 Theory Setup procedures. Troubleshooting the 2690/5 User maintenance of the 2690/5 Spare Parts 2 2690/5 Theory 2690/5 Solvent
More informationHousehold Acids and Bases
Household Acids and Bases GRADE LEVEL INDICATORS Experiment Demonstrate that the ph scale (0-14) is used to measure acidity and classify substances or solutions as acidic, basic, or neutral. 21 Develop
More informationPROTEINS (LOWRY) PROTOCOL
1 PROTEINS (LOWRY) PROTOCOL 1. INTRODUCTION The Lowry Assay: Protein by Folin Reaction (Lowry et al., 1951) has been the most widely used method to estimate the amount of proteins (already in solution
More information5.0 EXPERIMENT ON DETERMINATION OF TOTAL HARDNESS
5.0 EXPERIMENT ON DETERMINATION OF TOTAL HARDNESS Sl. No. Contents Preamble 5.1 Aim 5.2 Introduction 5.2.1 Environmental Significance 5.3 Principle 5.4 Materials Required 5.4.1 Apparatus Required 5.4.2
More informationColor, True and Apparent
Color, True and Apparent DOC316.53.01037 Platinum-Cobalt Standard Method 1, 2, 3 Method 8025 15 to 500 color units Scope and application: For water, wastewater and seawater; equivalent to NCASI method
More informationUSEPA 1 FerroVer Method 2 Method 8008 0.02 to 3.00 mg/l Fe Powder Pillows or AccuVac Ampuls
Iron, Total DOC316.53.01053 USEPA 1 FerroVer Method 2 Method 8008 0.02 to 3.00 mg/l Fe Powder Pillows or AccuVac Ampuls Scope and application: For water, wastewater and seawater; digestion is required
More information4.0 EXPERIMENT ON DETERMINATION OF CHLORIDES
4.0 EXPERIMENT ON DETERMINATION OF CHLORIDES Sl. No. Contents Preamble 4.1 Aim 4.2 Introduction 4.2.1 Environmental Significance 4.3 Principle 4.4 Materials Required 4.4.1 Apparatus Required 4.4.2 Chemicals
More informationExperiment 17: Potentiometric Titration
1 Experiment 17: Potentiometric Titration Objective: In this experiment, you will use a ph meter to follow the course of acid-base titrations. From the resulting titration curves, you will determine the
More informationDirect ISE Method Method 8359 10 to 1000 mg/l Na + Sodium ISE
Sodium DOC316.53.01240 Direct ISE Method Method 8359 10 to 1000 mg/l Na + Sodium ISE Scope and application: For drinking water and process water. Test preparation Instrument-specific information This procedure
More informationColor, True and Apparent
Color, True and Apparent DOC316.53.01037 Platinum-Cobalt Standard Method 1, 2, 3 Method 8025 15 to 500 color units 5 to 500 color units (low range technique) Scope and application: For water, wastewater
More informationEvaluation copy. Case File 9. A Killer Cup of Coffee? GlobalTech manager dies
Case File 9 Killer Cup of Coffee: Using colorimetry to determine concentration of a poison Determine the concentration of cyanide in the solution. A Killer Cup of Coffee? SOUTH PAINTER, Tuesday: It was
More informationCHEMICAL DETERMINATION OF EVERYDAY HOUSEHOLD CHEMICALS
CHEMICAL DETERMINATION OF EVERYDAY HOUSEHOLD CHEMICALS Purpose: It is important for chemists to be able to determine the composition of unknown chemicals. This can often be done by way of chemical tests.
More informationHousehold Acids and Bases
Household Acids and Bases Computer 28 Many common household solutions contain acids and bases. Acid-base indicators, such as litmus and red cabbage juice, turn different colors in acidic and basic solutions.
More informationAIRFREE TECHNIQUE AND SENSITIVE REAGENTS S ECTI O N 1: GLASS W ARE A ND E Q UIP M ENT. A. Using a manifold
AIRFREE TECHNIQUE AND SENSITIVE REAGENTS S ECTI O N 1: GLASS W ARE A ND E Q UIP M ENT Some organic compounds are air sensitive. They can react with the water vapor or oxygen in the air. In order to perform
More informationNeutralizing an Acid and a Base
Balancing Act Teacher Information Objectives In this activity, students neutralize a base with an acid. Students determine the point of neutralization of an acid mixed with a base while they: Recognize
More informationSyllabus OC18 Use litmus or a universal indicator to test a variety of solutions, and classify these as acidic, basic or neutral
Chemistry: 9. Acids and Bases Please remember to photocopy 4 pages onto one sheet by going A3 A4 and using back to back on the photocopier Syllabus OC18 Use litmus or a universal indicator to test a variety
More informationDetermining the Free Chlorine Content of Swimming Pool Water. HOCl H + + OCl. Evaluation copy
Determining the Free Chlorine Content of Swimming Pool Water Computer 33 Physicians in the nineteenth century used chlorine water as a disinfectant. Upon the discovery that certain diseases were transmitted
More informationTo see how this data can be used, follow the titration of hydrofluoric acid against sodium hydroxide below. HF (aq) + NaOH (aq) H2O (l) + NaF (aq)
Weak Acid Titration v120413 You are encouraged to carefully read the following sections in Tro (2 nd ed.) to prepare for this experiment: Sec 4.8, pp 158-159 (Acid/Base Titrations), Sec 16.4, pp 729-43
More informationACETALDEHYDE and ISOVALERALDEHYDE (Gas Chromatography)
ACETA.02-1 ACETALDEHYDE and ISOVALERALDEHYDE (Gas Chromatography) PRINCIPLE Isovaleraldehyde (IVA) and acetaldehyde are released from the syrup with the aid of dilute phosphoric acid and heat. The liberated
More informationLead Testing and On Site Calibration for Water Testing Detection Range: 2 100ppb
Document: AND Lead 100 7 2013 Lead Testing and On Site Calibration for Water Testing Detection Range: 2 100ppb July, 2013 Edition 1 ANDalyze, Inc., 2012. All rights reserved. Printed in USA. Table of Contents
More informationCary 100 Bio UV-Vis Operating Instructions 09/25/2012 S.V.
1234 Hach Hall 515-294-5805 www.cif.iastate.edu Cary 100 Bio UV-Vis Operating Instructions 09/25/2012 S.V. Location: Contact: 1240 Hach Hall Steve Veysey, 1234 Hach Hall Safety All researchers working
More informationDimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856
Nickel DOC316.53.01065 Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856 Scope and application: For water and wastewater. Test preparation Instrument-specific information Table 1 shows
More informationJuice Titration. Background. Acid/Base Titration
Juice Titration Background Acids in Juice Juice contains both citric and ascorbic acids. Citric acid is used as a natural preservative and provides a sour taste. Ascorbic acid is a water-soluble vitamin
More informationColorimetric Determination of Iron in Vitamin Tablets
Colorimetric Determination of Iron in Vitamin Tablets Big Picture Conceptual Approach Vitamin Tablet How much Fe? ph = 3.5 Vitamin Tablet How much Fe? Too difficult to eyeball so will have the colorimeter
More informationExperiment 7: Titration of an Antacid
1 Experiment 7: Titration of an Antacid Objective: In this experiment, you will standardize a solution of base using the analytical technique known as titration. Using this standardized solution, you will
More informationSupport Cleaning Apparatus. Operation, Cleaning, & Safety Manual
Support Cleaning Apparatus Operation, Cleaning, & Safety Manual Revision 1.1 31 May 2014 WARNING: The instructions and precautionary statements contained herein are intended for the Support Cleaning Apparatus
More informationANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE
ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of total nitrogen in food and crude protein calculation (Kjeldahl method) Responsible person: Assoc.Prof. Ing.Kateřina Riddellová,
More informationFerroVer Method 1 Method 10249 0.1 to 3.0, 1.0 to 30.0 and 10.0 to 300.0 mg/l Fe Powder Pillows
Iron, Total DOC316.53.01314 FerroVer Method 1 Method 10249 0.1 to 3.0, 1.0 to 30.0 and 10.0 to 300.0 mg/l Fe Powder Pillows Scope and application: For oil and gas field waters; digestion is required for
More information2. SUMMARY 3. DEFINITIONS
Determination of Total Dissolved Phosphorus (TDP) in Fresh/Estuarine/Coastal Waters Using Alkaline Persulfate Digestion of Phosphorus to Orthophosphate (PO4) 1. SCOPE and APPLICATION 1.1 Potassium persulfate
More informationExperiment 16-Acids, Bases and ph
Definitions acid-an ionic compound that releases or reacts with water to form hydrogen ion (H + ) in aqueous solution. They taste sour and turn litmus red. Acids react with certain metals such as zinc,
More informationAscorbic Acid Titration of Vitamin C Tablets This lab will be completed individually! Make sure you come prepared!
Ascorbic Acid Titration of Vitamin C Tablets This lab will be completed individually! Make sure you come prepared! Introduction Vitamin C (also known as ascorbic acid, HC6H7O6) is a necessary ingredient
More informationQuaternary Ammonium Compounds DOC316.53.01128
Quaternary Ammonium Compounds DOC316.53.01128 Direct Binary Complex Method Method 8337 0.2 to 5.0 mg/l as CTAB (cetyl-trimethylammonium bromide) Powder Pillows Scope and application: For cooling tower
More informationAcids and Bases. AND a widemouth container of the following solids:
Acids and Bases GOAL To introduce students to acids and bases. MATERIALS: 3 10oz clear plastic cups 1 4 oz. bottle white vinegar - labeled Acid 1 4 oz. bottle of water - labeled Water 1 4 oz. bottle of
More informationEXPERIMENT 4. Determination of Sodium by Flame Atomic-Emission Spectroscopy
EXPERIMENT 4 Determination of Sodium by Flame Atomic-Emission Spectroscopy USE ONLY DEIONIZED WATER (NOT DISTILLED WATER!) THROUGHOUT THE ENTIRE EXPERIMENT Distilled water actually has too much sodium
More informationCreatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07
Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3
More informationAcid Dissociation Constants and the Titration of a Weak Acid
Acid Dissociation Constants and the Titration of a Weak Acid One of the most important applications of equilibria is the chemistry of acids and bases. The Brønsted-Lowry acid-base theory defines an acid
More informationProperties of Acids and Bases
Lab 22 Properties of Acids and Bases TN Standard 4.2: The student will investigate the characteristics of acids and bases. Have you ever brushed your teeth and then drank a glass of orange juice? What
More informationEvaluation copy. Titration of a Diprotic Acid: Identifying an Unknown. Computer
Titration of a Diprotic Acid: Identifying an Unknown Computer 25 A diprotic acid is an acid that yields two H + ions per acid molecule. Examples of diprotic acids are sulfuric acid, H 2 SO 4, and carbonic
More informationDetermination of calcium by Standardized EDTA Solution
Determination of calcium by Standardized EDTA Solution Introduction The classic method of determining calcium and other suitable cations is titration with a standardized solution of ethylenediaminetetraacetic
More informationVitamin C Content of Fruit Juice
1 Vitamin C Content of Fruit Juice Introduction Vitamin C Vitamins are organic compounds that have important biological functions. For instance, in humans they enable a variety of enzymes in the body to
More informationMicromass LCT User s Guide
Micromass LCT User s Guide 1) Log on to MassLynx with your username & password. 2) After you have logged in, the MassLynx software will automatically run. 3) After MassLynx has come up, open your project
More informationDissolving of sodium hydroxide generates heat. Take care in handling the dilution container.
TITRATION: STANDARDIZATION OF A BASE AND ANALYSIS OF STOMACH ANTACID TABLETS 2009, 1996, 1973 by David A. Katz. All rights reserved. Reproduction permitted for education use provided original copyright
More informationCalibration of Glassware
Calibration of Glassware Introduction Glassware is commonly calibrated using a liquid of known, specific density, and an analytical balance. The procedure is to determine the mass of liquid the glassware
More informationAbsorbance Spectrophotometry: Analysis of FD&C Red Food Dye #40
Absorbance Spectrophotometry: Analysis of FD&C Red Food Dye #40 Note: there is a second document that goes with this one! 2046 - Absorbance Spectrophotometry - Calibration Curve Procedure. The second document
More informationph Measurements of Common Substances
Chem 100 Section Experiment 10 Name Partner s Name Introduction ph Measurements of Common Substances The concentration of an acid or base is frequently expressed as ph. Historically, ph stands for the
More informationYSI Meter Protocol: Specific Conductivity, ph, & Dissolved Oxygen
YSI Meter Protocol: Specific Conductivity, ph, & Dissolved Oxygen 1. Check the log-book to see if somebody else has already checked the meter for accuracy within the timeframes below. If the meter hasn
More informationGETTING STARTED. 6. Click the New Specimen icon.
BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified
More informationOXIDATION-REDUCTION TITRATIONS-Permanganometry
Experiment No. Date OXIDATION-REDUCTION TITRATIONS-Permanganometry INTRODUCTION Potassium permanganate, KMnO 4, is probably the most widely used of all volumetric oxidizing agents. It is a powerful oxidant
More informationColorimetric Determination of Iron in Vitamin Tablets
Cautions: 6 M hydrochloric acid is corrosive. Purpose: To colorimetrically determine the mass of iron present in commercial vitamin tablets using a prepared calibration curve. Introduction: Iron is considered
More information18 Conductometric Titration
Lab Activity 18 CONDUCTOMETRIC TITRATION LAB ACTIVITY 18 Conductometric Titration Background Titration is the a method of determining the concentration of an unknown solution (the analyte) by reacting
More informationNeutralization Reactions. Evaluation copy
Neutralization Reactions Computer 6 If an acid is added to a base, a chemical reaction called neutralization occurs. An example is the reaction between nitric acid, HNO 3, and the base potassium hydroxide,
More informationRelated concepts Kohlrausch s law, equivalent conductivity, temperature dependence of conductivity, Ostwald s dilution law.
Conductivity of strong and weak electrolytes TEC Related concepts Kohlrausch s law, equivalent conductivity, temperature dependence of conductivity, Ostwald s dilution law. Principle It is possible to
More informationPROCEDURE COVERSHEET. Document Section: CHEMISTRY Author: JOANNE WONG Date Authored: JAN 2011. In Use Date: 2/1/2011 Discontinued Date:
PROCEDURE COVERSHEET Procedure Title: ph (Urine, Body Fluid, Platelet Samples) Document Section: CHEMISTRY Author: JOANNE WONG Date Authored: JAN 2011 Laboratory Director: TED KURTZ, MD (PARNASSUS & CHINA
More informationMeasuring ph in Water or CaCl2 Using a ph Meter. Compiled by Darren Murray, June 30, 2011 TABLE OF CONTENTS OVERVIEW 3
Measuring ph in Water or CaCl2 Using a ph Meter Compiled by Darren Murray, June 30, 2011 TABLE OF CONTENTS OVERVIEW 3 PREPARING THE ph METER FOR USE (Calibration and Buffer Solutions) 4 When is Calibration
More informationInc. Wuhan. Quantity Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4 Standard (liquid) 2
Uscn Life Science Inc. Wuhan Website: www.uscnk.com Phone: +86 27 84259552 Fax: +86 27 84259551 E-mail: uscnk@uscnk.com ELISA Kit for Human Prostaglandin E1(PG-E1) Instruction manual Cat. No.: E0904Hu
More informationFree SO 2. by Aeration-Oxidation. A n o t h e r M o r e M a n u a l!
Free SO 2 by Aeration-Oxidation A n o t h e r M o r e M a n u a l! Provided by MoreWine! A MoreFlavor!, Inc. Brand www.morewinemaking.com 1-800-600-0033 H570 MT116 MT104 D1702 MT117 R335 D1702 Kit Item
More informationMeasurement and Calibration
Adapted from: H. A. Neidig and J. N. Spencer Modular Laboratory Program in Chemistry Thompson Learning;, University of Pittsburgh Chemistry 0110 Laboratory Manual, 1998. Purpose To gain an understanding
More informationUscn Life Science Inc. Wuhan
Uscn Life Science Inc. Wuhan Website: www.uscnk.com Phone: +86 27 84259552 Fax: +86 27 84259551 E-mail: uscnk@uscnk.com ELISA Kit for Human Creatine Kinase,Mitochondrial 1A (CKMT1A) Instruction manual
More informationPaper Chromatography: Separation and Identification of Five Metal Cations
Paper Chromatography: Separation and Identification of Five Metal Cations Objectives Known and unknown solutions of the metal ions Ag +, Fe 3+, Co 2+, Cu 2+ and Hg 2+ will be analyzed using paper chromatography.
More informationph units constitute a scale which allows scientists to determine the acid or base content of a substance or solution. The ph 0
ACID-BASE TITRATION LAB PH 2.PALM INTRODUCTION Acids and bases represent a major class of chemical substances. We encounter them every day as we eat, clean our homes and ourselves, and perform many other
More informationEnvironmental Water Testing: Surface Water, Groundwater, Hard Water, Wastewater, & Seawater
Document: AND Sol Env 08 2013 Environmental Water Testing: Surface Water, Groundwater, Hard Water, Wastewater, & Seawater Matrix specific sample preparation and testing methods for environmental waters
More informationPositive Test for Vitamin C. When a liquid containing Vitamin C is added to Indophenol Solution, the colour changes from Blue to Clear.
Sc ienc e 9-Biology Ex perim ent 6-4 Test ing for Vit am in C Name Due Date 10 Show Me Hand In Correct and Hand In Again By Purpose: To test some foods for the presence Vitamin C Materials: Small dropper
More informationAIRMoN, ph and Conductivity Field Measurement
AIRMoN, ph and Conductivity Field Measurement Items needed: Sample to be measured (sample volume 50 ml) Field Observer Form (FOF) for the sample to be measured conductance cell ph vials, 4 conductivity
More informationThe introduction of your report should be written on the on the topic of the role of indicators on acid base titrations.
Experiment # 13A TITRATIONS INTRODUCTION: This experiment will be written as a formal report and has several parts: Experiment 13 A: Basic methods (accuracy and precision) (a) To standardize a base (~
More informationVAPOR PRESSURE AS A FUNCTION OF TEMPERATURE. This laboratory covers material presented in section 11.8 of the 9 th Ed. of the Chang text.
VAPOR PRESSURE AS A FUNCTION OF TEMPERATURE Objectives: (1) Observe and measure the change in the vapor pressure (dependent variable) as a function of temperature (independent variable). (2) Analyze the
More informationExperiment 8 - Double Displacement Reactions
Experiment 8 - Double Displacement Reactions A double displacement reaction involves two ionic compounds that are dissolved in water. In a double displacement reaction, it appears as though the ions are
More information15. Acid-Base Titration. Discover the concentration of an unknown acid solution using acid-base titration.
S HIFT INTO NEUTRAL 15. Acid-Base Titration Shift into Neutral Student Instruction Sheet Challenge Discover the concentration of an unknown acid solution using acid-base titration. Equipment and Materials
More informationAcids & Bases Around the House Use a ph indicator to find acids and bases
Use a ph indicator to find acids and bases Description: Visitors predict whether various household solutions are acids or bases, and test their hypotheses using a universal ph indicator. Then, visitors
More informationCHEM 161: Beer s Law and Analysis of a Sports Drink
CHEM 161: Beer s Law and Analysis of a Sports Drink Introduction Although sunlight appears white, it contains a spectrum of colors. A rainbow actually shows this range of colors in visible light: violet,
More informationChemistry 119: Experiment 7. Potentiometric Titration of Ascorbic Acid in Vitamin C Tablets
Chemistry 119: Experiment 7 Potentiometric Titration of Ascorbic Acid in Vitamin C Tablets Vitamin C is another name for ascorbic acid (C 6 H 8 O 6, see below ), a weak acid that can be determined by titration
More informationVolumetric Analysis. Lecture 5 Experiment 9 in Beran page 109 Prelab = Page 115
Volumetric Analysis Lecture 5 Experiment 9 in Beran page 109 Prelab = Page 115 Experimental Aims To prepare and standardize (determine concentration) a NaOH solution Using your standardized NaOH calculate
More informationCONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in
More information