Zoonózy: riziko představované potravinami rostlinného původu Zoonosen: ein Risiko für Pflanzenlebensmitteln
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- Kenneth Strickland
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1 Zoonózy: riziko představované potravinami rostlinného původu Zoonosen: ein Risiko für Pflanzenlebensmitteln Prof. MVDr. Pavlík, CSc., Mgr. Petra Vašíčková, PhD., MVDr. Alena Lorencová, PhD., Ing. Hana Vojkovská Výzkumný ústav veterinárního lékařství, v.v.i., Brno Dolní Dunajovice,
2 Veterinary Research Institute, Brno
3 Departments 1. Virology and diagnostics 2. Bacteriology 3. Immunology 4. Food and Feed Safety 5. Genetics and Reproduction 6. Chemistry and Toxicology
4 Units: Food and Feed Safety Department 1. Food microbiology 2. Food virology 3. Zoonoses (tuberculosis, salmonellas, ) 4. Feed and health of farm animals 5. Molecular biology World Organisation for Animal Health RL for: 1. Paratuberculosis 2. Avian tuberculosis
5 RL for Paratuberculosis Since 2003
6 RL for Avian Tuberculosis Since 2005
7 Mycobacteriology Unit (1995) Methodical and Consulting Centre of SVA (1996)
8 Communication with the field About 700 reports to the field yearly Accredited laboratory for the diagnostics of mycobacterial infections in animals since 2002
9 OP VaVpI AmireVet ( )
10 AdmireVet (15,0 mil EUR) Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíně Center for: Advanced Microbiology & Immunology Reserach in Veterinary Medicine
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14 Potentially pathogenic microorganisms Main source infected animals and/or humans Food products milk, meat Less attention paid to food from plant origin
15 History of transmitted zoonotic agents by fruit and vegetable in the Czech Republic?
16 Tularemia
17 Open gate for Francisella tularensis
18 EPIDAT, SZÚ, Praha
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22 Risk perception
23 History in the Czech Republic
24 150 patients with pulmonar tularemia sugar factory sugar beet washing
25 48 patients (36 adult and 12 children) drinking water from the well
26 129 patients (35 employees and 94 old people) Apple juice
27 47 positive reagents from 950 examined
28 71 positive reagents from 1291 examined
29 71 positive reagents from 1380 examined
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31 Fertilization with human dung-water (waste) in Poland
32 EPIDAT, SZÚ, Praha
33 EPIDAT, SZÚ, Praha
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35 Biogass
36 Temperatur es
37 First experiments in our laboratory
38 M. a. paratuberculosis contamination of soil (CFU) Material Lettuce Radish Tom ato Soil surface middle bottom Roots Leaves lower middle 1 nt 0 upper Shoot surface Stalk inside 0 2 2
39 In vitro experiments on intact plants MAH field isolate, input 10 8 cells/ml of media
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41 Results: qpcr (IS1245) Cultivation Peas Stem (1) 4,61 x 10 4 /g + (10) Stem (2) 2,64 x 10 4 /g + (50) Beans Stem (1) 6,31 x 10 6 /g + (10) Leaf (1) 6,70 x 10 6 /g + (70) Stem (2) 1,54 x 10 7 /g + (10) Leaf (2) 1,74 x 10 6 /g + (100) Stem (3) 7,74 x 10 6 /g + (50) Leaf (3) 3,49 x 10 6 /g + (50)
42 Current situation in the USA and EU
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44 The Community Summary Report on trends and sources of zoonoses, zoonotic agents and foodborne Outbreaks in the European Union in 2008, EFSA
45 EPIDAT, SZÚ, Praha
46 EPIDAT, SZÚ, Praha
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54 EFSA 2007
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60 Food chain - fruit
61 Collected samples
62 Companies (Foto T. Petrovic a A. Rzezutka)
63 Fresh/frozen fruit
64 Výsledky - produkce Vzorek hadv padv bpyv NoV HAV HEV Ruce 14/200 0/117 0/117 0/117 0/117 nt Latríny 2/28 nt nt 0/13 0/13 nt Kliky latrín 2/28 nt nt 0/14 0/14 nt Voda 9/108 5/90 1/90 2/41 0/56 0/56 Hnojivo nt 4/7 0/7 nt nt 0/4 AdV-adenovirus, bpyv-polyomavirus, NoV-norovirus, HAV-virus hepatitidy A, HEVvirus hepatitid E, nt-netestováno
65 Výsledky zpracování a prodej Vzorek hadv padv bpyv NoV HAV HEV Ruce 1/50 nt nt 0/1 0/1 nt Transport. pás Plody čerstvé Plody mražené 0/63 nt nt 0/24 0/24 nt 2/203 4/121 0/121 0/81 0/81 0/81 3/96 1/77 0/77 0/39 0/39 1/39 AdV-adenovirus, bpyv-polyomavirus, NoV-norovirus, HAV-virus hepatitidy A, HEVvirus hepatitid E, nt-netestováno
66 Výsledky ČR AdV-adenovirus, bpyv-polyomavirus, NoV-norovirus, HAV-virus hepatitidy A, HEVvirus hepatitid E, nt-netestováno
67 Výsledky ČR AdV-adenovirus, bpyv-polyomavirus, NoV-norovirus, HAV-virus hepatitidy A, HEVvirus hepatitid E, nd-netestováno
68 Surface water risk factor
69 Fertilization with organic waste products
70 Total fermentation
71 Sewage and liquid manure application
72 EPIDAT, SZÚ, Praha
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74 EPIDAT, SZÚ, Praha
75 Future risks?
76 Raw meat consumption... 76
77 New consumers behavior is expected New risks are expected
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80 Contaminated surface water
81 New risks connected with changed ecology conditions
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84 Water washed lettuce
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87 Soil contamination
88 Straw
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90 Peat Matlova et al., J. Clin. Microbiol., 2005,
91 Children neck tuberculosis
92 Infected sellers / food handlers / field workers...
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94 Clear instructions
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96 Personal hygiene
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98 EPIDAT, SZÚ, Praha
99 VÚVeL Brno, 2008, kick off meeting 99
100 Katedra za biotehnologijo, Biotehniška fakulteta, Univerza v Ljubljani, peter.raspor@bf.uni-lj.si We need innovations in food production chain!! AGRICULTURE PRODUCTION DISTRIBUTION RETAIL CONSUMPTION F o o d Farm Assurance Schemes HACCP based Production QA Chill Chain and Handling In store HACCP Home? Traceability and Control
101 Codex Alimentarius can help
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106 This work was supported by grants Nos. 1B0078 and MZE of Min. of Agr. of the Czech Republic Děkuji za pozornost
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