ADJUNCTIVE THERAPIES FOR TYPE 1 DIABETES



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ADJUNCTIVE THERAPIES FOR TYPE 1 DIABETES Dr. Mohammad Alhadj Ali, MD, PgDip, MSc, PhD (UK) with Prof. David Owens (UK) Outline Type 1 Diabetes Immunology of Type 1 Diabetes Treatment of Type 1 Diabetes Adjunct non-insulin therapies in Type 1 Diabetes Future directions

Type 1 Diabetes 5-10 % of cases of diabetes Affecting 18 to 20/100 000 children/year in UK ~50% diagnosed > 18 years Particular HLA molecules are associated with susceptibility to T1DM (HLA-DR4 and HLA-DQ8) Outline Type 1 Diabetes Immunology of Type 1 Diabetes Treatment of Type 1 Diabetes Adjunct non-insulin therapies in Type 1 Diabetes Future directions

Pathogenesis of Type 1 Diabetes T-cell Infiltration Islet Destruction Immunology Type 1 Diabetes Type 1 diabetes is an autoimmune disease Auto-reactive T-cells are involved in destruction of b-cells Abs to insulin, GAD and IA2 precede T1DM 10 y or more 20% High risk children have Abs by age 2

Outline Type 1 Diabetes Immunology of Type 1 Diabetes Treatment of Type 1 Diabetes Adjunct non-insulin therapies in Type 1 Diabetes Future directions Treatment of Type 1 Diabetes On January 23, 1922, at the University of Toronto, Dr. Frederick Banting and his student C. H. Best made one of the greatest discoveries of the 20 th century.

Insulin in Type 1 Diabetes It has been used for diabetes treatment for more then 90 years It is effective in lowering blood glucose Side effects are not common It is still a cornerstone in the treatment of T1DM Insulin in Type 1 Diabetes

Barriers to Treatment with Insulin Patients Fear of injections and insulin Fear of hypoglycaemia Weight gain Inconvenience Physical (hearing and visual problems) Mental (learning difficulties) Suppliers Cost and expenses Time constraints Patient s compliance Physicians inertia Support and service Follow up Advances in Insulin Therapy Newer insulin Other forms of insulin (oral, nasal..) New delivery systems Advanced glucose monitoring systems The closed-loop pumps and the artificial pancreas

The Challenge in treating T1DM Alhadj Ali et al, 2009, BJDVD. 248-253. Outline Type 1 Diabetes Immunology of Type 1 Diabetes Treatment of Type 1 Diabetes Adjunct non-insulin therapies in Type 1 Diabetes Future directions

Goals of Adjunct Non-insulin Therapies in T1DM Improve glycaemic control Improve patient s safety and reduces hypoglycaemia Preserve endogenous β-cell function To compliment rather than necessarily replace insulin therapy Reduce insulin resistance and weight gain George et al, 2012, Diabetic Medicine. 179-188.

Adjunctive-non insulin therapy in T1DM Adjunctive therapy in C-peptide positive T1DM Adjunctive therapy in C-peptide negative T1DM Adjunct Non-insulin-based Therapies in C-peptide Negative T1DM The focus of these therapies is to improve the glycaemic control by using treatments to address some of the metabolic and physiological abnormalities which are less responsive to insulin replacement therapy. Adjunctive therapy in C-peptide negative T1DM Improve glycaemic control

Goals of Adjunct Non-insulin Therapies in T1DM Improve glycaemic control Preserve endogenous β-cell function To compliment rather than necessarily replace insulin therapy Reduce insulin dependence Metformin GLP-1 analogues DDP-4 inhibitors Adjunctive-non insulin therapy in T1DM Pioglitazone Amylin analogues α- glucosidase inhibitors SGLT-2 inhibitors

Adjunctive therapy in C-peptide negative T1DM Insulin sensitizing Targeting Hyperglucagonaemia Metformin Poglitazone α-glucosidase Inhibitors DPP-4 Inhibitors GLP-1 Analogues Amylin Analogues Metformin Actions Inhibits hepatic gluconeogenesis Improves insulin-mediated glucose utilization Reduces intestinal glucose absorption Reduces fatty acid oxidation Reduces LDL and VLDL

Metformin Potential benefits in T1DM Improves fasting and postprandial glycaemic profile Improves the overall glycaemic profile Reduces weight Insulin-sparing effect Improves the cardiovascular outcomes Standardised mean differences in HbA1c level between Metformin-treated and Metformin-free Type 1 Diabetes patients

Standardised mean differences in insulin dose between Metformin-treated and Metformin-free Type 1 Diabetes patients Pioglitazone Actions Improves glucose delivery to the periphery Decreases gluconeogenesis Potential benefits in T1DM Reduces insulin resistance Improves the overall glycaemic effect

Alpha-glucosidase Inhibitors Actions Inhibit digestion of disaccharide to monosaccharide Potential benefits in T1DM Improve the postprandial glycaemic profile Improve the overall glycaemic profile Insulin-sparing effect

DPP-4 inhibitors Actions Inhibit the endogenous GLP-1 breakdown Potential benefits in T1DM Improve the postprandial glycaemic profile Improve the overall glycaemic effect Insulin-sparing effect

GLP-1 Analogues Actions Increase the insulin secretion Regulate the postprandial glucagon release Delay the gastric emptying They have central effect on satiety

GLP-1 Analogues Potential benefits in T1DM Inhibit β-cell apoptosis Halt disease progression and reversal of disease Improve the postprandial and overall glycaemic profiles Reduce the glycaemic variability Reduces weight

Amylin Analogues Actions Inhibit hepatic gluconeogenesis Regulate the postprandial glucagon release Reduce gastric emptying They have central effect on satiety Potential benefits in T1DM Improve the postprandial and overall glycaemic profiles Reduce weight

SGLT-2 Inhibitors Actions Reduce renal glucose absorption in proximal renal tubules Potential benefits in T1DM Improve the overall glycaemic profiles Reduce weight

Adjunct Non-insulin-based Therapies in C-peptide Positive T1DM The focus of these therapies is to restore self-tolerance to pancreatic β-cells by controlling the autoimmune responses to these cells. Regulatory T Cells Autoimmunity Type 1 Diabetes Effector T Cells

Adjunct Non-insulin-based Therapies in C-peptide Positive T1DM The purpose of these therapies is to halt the ongoing autoimmune process, preventing β-cells from further destruction and allowing them for a potential regeneration. Regulatory T Cells Effector T Cells Alhadj Ali et al, 2009, BJDVD. 248-253.

Immunosuppression or Immunomodulation? De Filippo et al, 1988

Immunotherapy Treatment Strategies Antigen specific Immunomodulation Non-antigen specific Immunomodulation Oral and parental insulin Intranasal insulin GAD-65 DiaPep 277 Proinsulin peptide Systemic (Cyclosporine A) Stem cell transplant T cell-targeted therapy B cell-targeted therapy Cytokines-targeted therapy Staeva et al, 2013, Diabetes. Vol 62, 9-17.

2001 104 361-366 Antigen Specific Immunotherapy for Autoimmune Disease: Fighting Fire with Fire? Colin M Dayan and Mark Peakman Monday, 13 December, 2004, 10:44 GMT Diabetes vaccine trials to begin A vaccine that could cure Type 1 diabetes is to be tested on people for the first time. It will be of help for people who have just been diagnosed Dr Colin Dayan from the University of Bristol

Outline Type 1 Diabetes Immunology of Type 1 Diabetes Treatment of Type 1 Diabetes Adjunct non-insulin therapies in Type 1 Diabetes Future directions Future Directions Combined treatments that act on insulin sensitizing with agents that target hyperglucagonaemia Combined immunotherapies in new-onset T1DM Emerging immunotherapies and pancreatic islet transplantation Dual-hormone artificial pancreas Stem cells and β cells regeneration

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