Axumin (fluciclovine F 18) Injection Case Studies

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Axumin (fluciclovine F 18) Injection Case Studies November, 2016

Axumin (fluciclovine F 18) Injection Indication Axumin (fluciclovine F 18) injection is indicated for positron emission tomography (PET) imaging in men with suspected prostate cancer recurrence based on elevated blood prostate specific antigen (PSA) levels following prior treatment. 1

Axumin (fluciclovine F 18) Injection Important Safety Information Image interpretation errors can occur with fluciclovine PET imaging. A negative image does not rule out recurrent prostate cancer and a positive image does not confirm its presence. The performance of fluciclovine seems to be affected by PSA levels. Fluciclovine uptake may occur with other cancers and benign prostatic hypertrophy in primary prostate cancer. Clinical correlation, which may include histopathological evaluation, is recommended. Hypersensitivity reactions, including anaphylaxis, may occur in patients who receive fluciclovine. Emergency resuscitation equipment and personnel should be immediately available. Fluciclovine use contributes to a patient s overall long-term cumulative radiation exposure, which is associated with an increased risk of cancer. Safe handling practices should be used to minimize radiation exposure to the patient and health care providers. Adverse reactions were reported in 1% of subjects during clinical studies with fluciclovine. The most common adverse reactions were injection site pain, injection site erythema and dysgeusia. To report suspected adverse reactions to Axumin, call 1-855-AXUMIN1 (1-855-298-6461) or contact FDA at 1-800-FDA-1088 or www.fda.gov/medwatch. Please see full prescribing information at www.axumin.com. 2

Important Information These case studies are being provided to you as examples of the images and information available following Axumin PET/CT imaging in patients with biochemically recurrent prostate cancer. The responsibility for the accurate and timely acquisition and interpretation of images using Axumin PET/CT scanning rests with the nuclear medicine physician or radiologist supervising the PET/CT imaging facility. These case studies are not intended to substitute for the independent medical judgment of the physician(s) responsible for the individual patient s management, nor are they a guarantee of any specific clinical results. Incidental findings are noted in some of the cases, as examples of potential, unanticipated abnormalities that may be identified during interpretation of Axumin images. The diagnostic efficacy of Axumin for the identification of these incidental abnormalities has not been established and confirmatory testing may be considered appropriate. 3

Case 1 - Overview Clinical History Age 57 Prior Therapy PSA Reason for Scan Acquisition Radical prostatectomy and bilateral staging pelvic lymphadenectomy 0.41 ng/ml PET/CT GE Discovery 690 Weight Administered Activity Time After Administration Reconstruction Suspected recurrent Prostate Cancer 98 kg 355MBq 5m 27s VPFX Institution Name: Department of Radiology and Imaging Sciences, Emory University, Atlanta, GA, USA 4

Case 1 SUV Figure 2: Internal iliac/pelvic side wall lymph node. Figure 1: MIP (Click to Play) (PET (Top Left) & PET/CT (Top Right) transaxial and PET/CT (Bottom Left) sagittal and (Bottom Right) coronal) LN SUV(bw) max : 11.9 Marrow (L3) SUV(bw) mean : 3.4 5

Case 1 Figure 3: Adrenal activity - normal variant (PET/CT transaxial) Figure 4: Rectal activity (PET sagittal) Figure 4: Benign lymphoceles (PET/CT transaxial) Figure 5: Benign bilateral inguinal nodes (PET (Top) transaxial and (Bottom) coronal) 6

Case 1 - Results Imaging Results Summary Internal iliac/pelvic side wall lymph node Incidental findings - Benign bilateral inguinal nodes. - Benign bilateral lymphoceles. - Adrenal activity - normal variant. - Rectal activity. NOTE: This case study is provided as an example of the interpretation and utility of Axumin PET/CT imaging results. Axumin is not indicated for directing or changing patient management. Such decisions must be based on the independent medical judgment of the physician(s) responsible for the individual patient s management. 7

Case 2 - Overview Clinical History Age Primary Stage Prior Therapy PSA Prior Imaging Reason for Scan 65 y.o. T3a N0 M0 Radiotherapy (EBRT/IMRT) 8 ng/ml Planar Bone Scan, CE-CT, Pelvic & Prostate MRI Suspected recurrent Prostate Cancer Acquisition PET/CT Siemens Biograph Truepoint Weight 75 kg Administered Activity 371MBq Time After Administration 4m 53s Reconstruction PSF, ToF, 2i21s Institution Name: Oslo University Hospital HF, Radiumhospitalet, Oslo 8

Case 2 SUV Figure 3: Retroperitoneal malignant node (PET/CT transaxial) LN SUV(bw) max : 8.3 LN short axis : 1.2 cm Marrow (L3) SUV(bw) mean : 4.0 Figure 1: MIP (Click to Play) Figure 2: Retroperitoneal malignant nodes (PET coronal) Figure 4: Thoracic malignant node (PET/CT transaxial) LN SUV(bw) max : 6.0 LN short axis : 0.6 cm Blood Pool (Aorta) SUV(bw) mean : 1.4 9

Case 2 SUV Figure 6: Benign appearing right iliac sclerosis (PET/CT (Top & Bottom Left) transaxials and CT (Bottom Right) coronal) Figure 7: Probable benign mild diffuse midline prostate activity (PET (Top) and PET-CT (Bottom) transaxials) 10

Case 2 SUV Figure 8: Incidental finding: Right arm vein focal activity (PET (Top) and PET-CT (Bottom) transaxials) Figure 5: Incidental finding: Left adrenal low density hyperplasia or adenoma (PET (Top) and CT (Bottom) transaxials) Adrenal SUV(bw) max : 3.5 Marrow (L3) SUV(bw) mean : 4.0 11

Case 2 - Results Imaging Results Summary Extensive retroperitoneal malignant nodes extending into thorax Probable benign mild diffuse midline prostate activity. Incidental: - Left adrenal hyperplasia/adenoma - Right arm vein focal activity - Benign appearing right iliac sclerosis. Management Plan Intended: Curative Revised: Palliative NOTE: This case study is provided as an example of the interpretation and utility of Axumin PET/CT imaging results. Axumin is not indicated for directing or changing patient management. Such decisions must be based on the independent medical judgment of the physician(s) responsible for the individual patient s management. 12

Case 3 - Overview Clinical History Age Primary Stage Prior Therapy PSA Gleason Prior Imaging Reason for Scan Acquisition PET/CT Weight Administered Activity Time After Administration Reconstruction 67 y.o. T2c N0 M0 Radiotherapy (EBRT/IMRT) and ADT 3.4 ng/ml 3+4 (biopsy) Planar bone scan; pelvic & prostate MRI (Extraprostatic region negative) Suspected recurrent Prostate Cancer Siemens Biograph Truepoint 96 kg 369 MBq 5m 9s PSF, ToF, 2i21s Institution Name: Oslo University Hospital HF, Radiumhospitalet, Oslo 13

Case 3 SUV Figure 1: MIP (Click to Play) Figure 2: Prostate (right apex) focal malignant uptake (PET (Left) coronal & (Bottom Right) transaxial & PET/CT (Top Right) transaxial) Prostate SUV(bw) max : 7.4 Marrow (L3) SUV(bw) mean : 3.7 14

Case 3 - Results Imaging Results Summary Prostate (right apex) focal uptake Histopathology Biopsy positive right apex Management Plan Intended:Curative salvage brachytherapy Revised: (No revision) PSA nadir <0.2 ng/ml NOTE: This case study is provided as an example of the interpretation and utility of Axumin PET/CT imaging results. Axumin is not indicated for directing or changing patient management. Such decisions must be based on the independent medical judgment of the physician(s) responsible for the individual patient s management. 15

Case 4 - Overview Clinical History Age 68 y.o. Primary Stage T3 (Pathological) N0 M0 Prior Therapy Radical prostatectomy PSA 3.9 ng/ml Gleason 4+5 Prior Imaging Bone Marrow MRI, Planar Bone Scan Reason for Scan Suspected recurrent prostate cancer Acquisition PET/CT Siemens Biograph Truepoint Weight 85 kg Administered Activity 318 MBq Time After Administration 4m 35s Reconstruction PSF, ToF, 2i21s Institution Name: Oslo University Hospital HF, Radiumhospitalet, Oslo 16

Case 4 SUV Figure 2: Bone L3 sclerotic malignant uptake Figure 1: MIP (Click to Play) (PET/CT (Top Left) transaxial & (Bottom Right) coronal & CT (Bottom Right) transaxial) L3 focal uptake SUV(bw) max : 7.6 Marrow (Normal) SUV(bw) mean : 3.8 17

Case 4 - Results Imaging Results Summary Bone L3 sclerotic malignant uptake Post-op bladder tracer activity. Incidental mucocele left maxillary sinus, right renal cysts. Follow-up Imaging Bone marrow MRI: Positive at L3 & also at T12 Management Plan Intended:Palliative Radiotherapy (EBRT/IMRT) to Prostate & Vesicles Revised: No revision NOTE: This case study is provided as an example of the interpretation and utility of Axumin PET/CT imaging results. Axumin is not indicated for directing or changing patient management. Such decisions must be based on the independent medical judgment of the physician(s) responsible for the individual patient s management. 18

Case 5 - Overview Clinical History Age Primary Stage Prior Therapy PSA 66 y.o. Gleason 4+5 Prior Imaging Reason for Scan Acquisition PET/CT Weight Administered Activity Time After Administration Reconstruction T2c (Pathological) N0 M0 Radical prostatectomy, negative lymphadenectomy 0.59 ng/ml Negative MRI (apart from Schwannoma right sacrum); planar bone scan Suspected recurrent Prostate Cancer Siemens Biograph Truepoint 122 kg 370 MBq 4m 52s PSF, ToF, 2i21s Institution Name: Oslo University Hospital HF, Radiumhospitalet, Oslo 19

Case 5 SUV Figure 2: Left presacral lymph node malignant uptake Figure 1: MIP (Click to Play) (CT (Top Left), PET (Bottom Left) & PET/CT (Top Right) transaxial & PET/CT (Bottom Right) coronal) Lymph Node SUV(bw) max : 6.7 Marrow (L3) SUV(bw) mean : 3.0 20

Case 5 Figure 3: Sclerotic lesions (fluciclovine negative) needing further evaluation (i.e. does not exclude metastases). (CT (Top) & PET/CT (Bottom) transaxials) 21

Case 5 Figure 4: Bladder activity (PET/CT (Top) transaxial & PET/CT (Bottom) sagittal) Figure 5: Mild benign inguinal nodal activity. (PET (Top) & PET/CT (Bottom) transaxials) 22

Case 5 - Results Figure 6: Benign left lymphocele. (PET/CT transaxial) Imaging Results Summary Intense small left presacral node (3x4 mm) Sclerotic lesions (fluciclovine negative) needing evaluation (i.e. does not exclude metastases). Mild symmetric inguinal nodal activity. Incidental: o Benign left lymphocele. o Sinus disease. o Bladder activity. Management Plan Intended: Curative - Radiotherapy Revised: Palliative - ADT Figure 7: Sinus disease NOTE: This case study is provided as an example of the interpretation and utility of Axumin PET/CT imaging results. Axumin is not indicated for directing or changing patient management. Such decisions must be based on the independent medical judgment of the physician(s) responsible for the individual patient s management. (PET transaxial) 23

Axumin (fluciclovine F 18) Injection Indication & Important Safety Information Indication Axumin (fluciclovine F 18) injection is indicated for positron emission tomography (PET) imaging in men with suspected prostate cancer recurrence based on elevated blood prostate specific antigen (PSA) levels following prior treatment. Important Safety Information Image interpretation errors can occur with fluciclovine PET imaging. A negative image does not rule out recurrent prostate cancer and a positive image does not confirm its presence. The performance of fluciclovine seems to be affected by PSA levels. Fluciclovine uptake may occur with other cancers and benign prostatic hypertrophy in primary prostate cancer. Clinical correlation, which may include histopathological evaluation, is recommended. Hypersensitivity reactions, including anaphylaxis, may occur in patients who receive fluciclovine. Emergency resuscitation equipment and personnel should be immediately available. Fluciclovine use contributes to a patient s overall long-term cumulative radiation exposure, which is associated with an increased risk of cancer. Safe handling practices should be used to minimize radiation exposure to the patient and health care providers. Adverse reactions were reported in 1% of subjects during clinical studies with fluciclovine. The most common adverse reactions were injection site pain, injection site erythema and dysgeusia. To report suspected adverse reactions to Axumin, call 1-855-AXUMIN1 (1-855-298-6461) or contact FDA at 1-800-FDA-1088 or www.fda.gov/medwatch. Please see full prescribing information at www.axumin.com. 24

Important Information These case studies are being provided to you as examples of the images and information available following Axumin PET/CT imaging in patients with biochemically recurrent prostate cancer. The responsibility for the accurate and timely acquisition and interpretation of images using Axumin PET/CT scanning rests with the nuclear medicine physician or radiologist supervising the PET/CT imaging facility. These case studies are not intended to substitute for the independent medical judgment of the physician(s) responsible for the individual patient s management, nor are they a guarantee of any specific clinical results. Incidental findings are noted in some of the cases, as examples of potential, unanticipated abnormalities that may be identified during interpretation of Axumin images. The diagnostic efficacy of Axumin for the identification of these incidental abnormalities has not been established and confirmatory testing may be considered appropriate. 25

END 26