Surgical training courses 2016

Surgical training courses 2016

Envigo and BioXpert have joined forces to provide a comprehensive range of educational courses for animal care professionals. This brochure includes the programs of practical training courses planned for 2016. The introduced 2010/63/EU Directive indicates that animal care employees shall be adequately educated and trained before they perform procedures on animals. Member States are obliged to publish their minimum requirements with regard to education and training and the requirements for obtaining, maintaining and demonstrating requisite competence. It s our responsibility to have appropriate training and competencies as they ensure best practice and solid results in the best interests of both our animals and scientific advancement. To maintain competencies, current levels of skill and knowledge must be regularly assessed. Therefore Envigo is offering a comprehensive range of training courses, which are discussed in this leaflet. Courses will be given in the Netherlands, in dedicated rooms for theory and practice with a strong focus on animal welfare, since good welfare is good science. Language: English Microscopes are equipped with additional oculars to enable our training team to see the participant s surgical field and give assistance. The surgical field of a demo microscope is displayed on a wide screen monitor to train specific parts of a training technique. All courses are given by experienced trainers and external experts with a laboratory animal science background, coordinated by an Animal Welfare Officer and/or FELASA D specialist. They aim to develop knowledge and skills for scientists and technicians with a focus on best practice and scientific excellence.

Introduction to cryopreservation This one-day seminar will cover theoretical information on cryobiology. The one-day training course closely examines: + Introduction to cryobiology: embryos, sperm and ovaries + Superovulation (administration and dosing) + Collection of oocytes + Use of cryoprotectants: permeable and non-permeable + Multiple stages of embryonic development + Freezing technologies: slow, rapid and vitrification + Containers: cryovials and straws + LN2 tanks We are facilitating these courses in the Netherlands, in dedicated rooms for theory and practice. Please note that a maximum of two participants will share her/his own set-up during the entire course, consisting of a dissection scope and several related tools. This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 795 (excl. VAT) Scheduled: April 7, 2016 Registration closes: March 24, 2016 Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance. In addition, basic embryo and sperm freezing (Jax vs. CARD methods) will be discussed.

Introductory course micro-surgery This two-day training course covers the basic principles of micro surgery in rodents including suture techniques. It will cover both theoretical information and practical hands-on surgery, and postsurgery handling and maintenance of the animals. The program includes: + Suture and dissection techniques using in silico + Suture techniques in anaesthetized rats, using a microscope + Preparation for catheter use This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 1,295, (excl. VAT) Scheduled: May 26-27, 2016 Registration closes: May 12, 2016 Registration fee includes two lunches, transportation from the hotel to the course venue, training materials and a certificate of attendance. + Anesthesia and jugular vein catheterization in rats + Aseptic working techniques + Individual additional techniques like ectomies or catheterization in rats and/or mice + Flushing of the catheter and locking of the catheter in order to prolong catheter patency

Advanced course on cryopreservation and embryo transfer in rodents This two-day advanced training course covers the basic principles of cryopreservation, embryo freezing and embryo transfer, as well as practical hands-on embryo collection, manipulation, freezing, thawing and culture. The program includes: + Introduction to cryobiology principles and freezing theory + Analysis of different types of cryoprotectants: permeable and non-permeable and their use + Different working protocols and freezing curves: slow, rapid and vitrification embryo freezing + Use of cryovials and straws + Pseudo pregnant production + Mouse superovulation (administration and dosing) We are facilitating these courses in the Netherlands, in dedicated rooms for theory and practice. Please note that a maximum of two participants will share her/his own set-up during the entire course, consisting of a dissection scope and several related tools. This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 1,595 (excl. VAT) Scheduled: July 7-8, 2016 Registration closes: June 23, 2016 Registration fee includes two lunches, transportation from the hotel to the course venue, training materials and a certificate of attendance. + Removal of presumptive zygotes + Multiple stages of embryonic development + Mouse euthanasia, embryo collection and embryo wash: the mouth pipetting technique + Embryo culture: analysis of different embryo culture media + Transfer of embryos into recipient animals

Necropsy and sample collection This one-day training course doesn t require basic surgical knowledge. The one-day training course will focus on: + Animal manipulation and euthanasia + Terminal procedures + Necropsy techniques, directed or total; the use of surgical instruments + Anatomy description: lymphatic nodes, different organs + Sample collection: blood and tissue + Fixers and perfusion + Biosafety We are facilitating these courses in the Netherlands, in dedicated rooms for theory and practice. Please note that a maximum of two participants will share her/his own set-up during the entire course, consisting of a dissection scope and several related tools. This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 795 (excl. VAT) Scheduled: Sept 29, 2016 Registration closes: Sept 15, 2016 Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance.

Reproductive surgery course This is a one-day hands-on training course. The one-day training course covers the basic principles of: + Anesthesia + Vasectomy + Pseudo-pregnant production + Embryo transfer: oviduct and uterine + Non-surgical embryo transfer (NSET) + Ovary transfer We are facilitating these courses in the Netherlands, in dedicated rooms for theory and practice. Please note that a maximum of two participants will share her/his own set-up during the entire course, consisting of a dissection scope and several related tools. This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 795 (excl. VAT) Scheduled: October 27, 2016 Registration closes: October 13, 2016 Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance.

Advanced course micro-surgery This two-day training course builds on the introductory course and continues with basic information on anesthesia and peri-operative care of small laboratory rodents. The practical part will focus on more advanced techniques. This course has a very strong focus on the individual participant with a lot of hands-on surgery. The program includes: + Anesthesia and peri-operative care with a focus on animal welfare + Bile fistula, portal vein and carotic artery techniques This guarantees personal attention and interaction during the training sessions, as well as time for individual practical discussions and training. Registration fee: 1,950 (excl. VAT) Scheduled: Nov 10-11, 2016 Registration closes: October 27, 2016 Registration fee includes two lunches, transportation from the hotel to the course venue, training materials and a certificate of attendance. + Portal vein catheterization in the rat + Individual training: additional techniques in mice or rats

Telemetry in rodents This two-day training course requires some basic skills of micro-surgery and aims to instruct participants on the applications of radio telemetry in small laboratory rodents both in demonstration and practical exercise. The program includes: + Aseptic working conditions + Surgical aspects reference ECG, BP and EEG transmitter implantation + Implantation ECG/BP dummy transmitters in the rat (abdominal aorta) + Implantation ECG/BP dummy transmitter in the mouse (carotid aorta and or abdominal aorta) This guarantees personal attention during the training session as well as time for individual practical discussions and training. Registration fee: 2,195 (excl. VAT) Scheduled: Nov 24-25, 2016 Registration closes: Nov 10, 2016 Registration fee includes two lunches, transportation from the hotel to the course venue, training materials and a certificate of attendance. Also included are the non-functional transmitters used during the course. + Implantation EEG dummy transmitters in the rat + Discuss surgical problems that can occur

Mouse sperm freezing This one-day training course is covering the theory and practice on sperm cryopreservation using the Jax and CARD methods. The one-day training course will focus on: + Introduction to sperm freezing and cryoprotectants theory + Collection and freezing of mouse sperm + Thawing + Sample evaluation + Theory on IVF We are facilitating these courses in the Netherlands, in dedicated rooms for theory and practice. Please note that a maximum of two participants will share her/his own set-up during the entire course, consisting of a dissection scope and several related tools. This guarantees personal attention during the training sessions as well as time for individual practical discussions and training. Registration fee: 795 (excl. VAT) Scheduled: Dec 8, 2016 Registration closes: Nov 24, 2016 Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance.

Invasive and/or semi-automated chronic blood sampling techniques in rodents Recent developments including the availability of pre-catheterized rodents and high sensitivity bio-analytics have enabled chronic, serial blood sampling in rodents. The automation of blood sampling and the use of dried blood spot techniques have simplified the collection of micro samples, making it possible to take a full PK profile from a single animal. However, the surgery itself and catheter patency remain as significant challenges. The one-day training course focuses on: + Surgery: catheterization techniques for chronic blood sampling + Jugular vein catheterization + Carotid artery catheterization + Materials + Balance arms and cage setup + Swivels + Catheters This guarantees personal attention and interaction during the training sessions, as well as time for individual practical discussions and training. Registration fee: Scheduled: 450 (excl. VAT) TBD Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance. + Advancements in automated blood sampling + Dry blood spot + 3Rs + Micro sampling + Connection methods; vascular access + Head attachment + Harness, vascular access harness + Button, vascular access harness

Injection and administration techniques in rodents This one-day training course is designed to improve the competence of laboratory animal technicians dealing with handling of rodents for purposes of injection. Both theoretical and practical training will be provided. Many technical skills can only be obtained by intensive and careful training. We invite attendees to share knowledge and expertise. Maximum number of attendees is fifteen persons. Registration fee: Scheduled: 450 (excl. VAT) TBD Registration fee includes lunch, transportation from the hotel to the course venue (when applicable), training materials and a certificate of attendance. The program includes: + Handling of mice, rats and guinea pigs + Injection techniques + Administration techniques

Dedicated courses We offer dedicated courses based on customer needs For example, you can be trained on specific skills such as stereotactic surgery, transverse aortic constriction (TAC), or ectomies. These courses are given in the Netherlands, however we can administer courses on site as well. Costs are dependent upon needs and location. For more information, contact us at or visit

Terms and conditions of registration The registration fees, materials and arrangements as indicated per training course in the brochure surgical training courses 2016 are applicable. Hotel and travel arrangements are the participant s responsibility. By sending in the registration form, you are automatically committed to its financial obligation as listed in our brochure surgical training courses 2016. You will then receive an acknowledgement of receipt and our invoice by email. The invoice needs to be fully paid before the start of the course. Payment is only possible by bank transfer. Final confirmation will be sent by email no later than 13 calendar days from the scheduled course date, together with the training program, details of the course venue, route description, etc. Envigo reserves the right to cancel or reschedule courses in the event the course leader or venue becomes unavailable due to circumstances beyond its control. Participants may cancel their course registration without penalty up to 28 calendar days from the scheduled course date. Late cancellations will be charged at 50% of the course fee as listed in our brochure surgical training courses 2016. Substitutions are welcome in lieu of cancellation up to one day prior to the scheduled course date with prior written notice to Envigo. In the event that Envigo is required to cancel or reschedule a course for which payment has already been received, the registrant will be offered a new course date or the ability to substitute another participant. If substitution of dates or attendees is not convenient, Envigo s sole liability will be a refund of the course fees that have been paid. Refunding will be at the discretion of Envigo. For economic reasons, Envigo reserves the right to combine different courses from our training program into the same session. In this case, our trainees and/or external experts will make sure that this will not interfere with the interest of the participant.

Surgical services Envigo offers a wide range of surgical services to assist customers with (micro) surgery in different kinds of animal models. In our surgical suites we produce standard and custom surgically modified animals with a focus on animal welfare. The surgery area is located next to the animal breeding barriers which enables us to have an efficient supply of animal models while maintaining a minimum risk of contamination. Surgically modified animals + Ectomies + Insertion of (non) vascular catheters + Connection of harnesses, buttons (jackets on request) + Soft-tissue procedures + Neurological procedures + Implantation of radio-telemetry transmitters + Implantation of other devices Sentinel animals are used to monitor the microbiological status of the surgically prepared animals in order to keep SPF status during and after surgery. Animals will be operated on according to the principles of good surgical practice, which include: + Aseptic working method + Minimal surgical trauma + Peri-operative care; also on request additional treatment + Short anesthesia and analgesia treatment + Detailed reporting; official animal diary + Detailed instructions for post-operative care All under guidance of our animal welfare officer.

2016 Envigo. RMS-0116-EU-01-SS-127