1.1. (Es)omeprazole and vitamin B12 deficiency



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1.1. (Es)omeprazole and Introduction Omeprazole, a substituted benzimidazole, and esomeprazole, the S-isomere of omeprazole belong to the class of proton pump inhibitors (PPIs) which strongly reduce gastric acid secretion by the parietal cell. The pharmacological mechanism of action is based on inhibiting the H + / K + -ATP-ase enzyme (the socalled proton pump) in the parietal cell of the stomach mucosa. Both the basal and the stimulated gastric acid secretion are dose dependently inhibited [1]. Proton pump inhibitors (PPIs) are widely approved for the treatment of oesophageal reflux disease, treatment and prophylaxis of (NSAID-associated) duodenal and benign gastric ulcers and relief of dyspeptic symptoms [2,3]. The net effect is a raise of the ph which can affect the absorption of other drugs and nutrients [1]. The pharmacodynamic activity of omeprazole and esomeprazole is comparable. Omeprazole has been registered since 1988 and is available as capsule, tablet and powder for infusion. Esomeprazole has been registered since 2000 and is available as tablet, capsules, granules for oral suspension, and powder for injection or infusion. Vitamin B12 is found only in food of animal origin such as meat, liver, and to a lesser extend dairy products. Vitamin B12 functions as a co-enzyme in the production of DNA. It is indispensable for the functioning of all organ systems, but the is reflected first in blood disorders. Other disorders, such as neurologic complications occur later on [1]. The current observation describes the association between (es)omeprazole and decreased serum levels and. Reports On July 26, 2011 the database of the Netherlands Pharmacovigilance Centre Lareb contained 4 reports concerning decreased levels or vitamin B12 (MedDRA preferred terms and decreased) with the use of (es)omeprazole. Specifications are shown in Table 1 below. Table 1. Reports of decreased associated with the use of (es)omeprazole Patient, Sex, Age, reporter Drug Indication for use Concomitant medication Suspected adverse drug reaction Time to onset, Action with drug outcome A, 110192 M 41-50 Consumer esomeprazole tablet enteric-coated 40 mg 2 dose not changed not recovered B 92278 M 51-60 Pharmacist esomeprazole entericcoated pravastatin tablet decreased; haemoglobin decreased 3 drug withdrawn recovered C 110469 F 31-40 Physician esomeprazole entericcoated 2dd1 prophylaxis NSAID NOS no information available

Patient, Sex, Age, reporter Drug Indication for use Concomitant medication Suspected adverse drug reaction Time to onset, Action with drug outcome D 102402 F 51-60 Pharmacist omeprazole capsule enteric-coated 40 mg nitrazepam tablet 5 mg bromazepam tablet 3 mg vitamine B12 7 dose not changed not recovered mirtazepine tablet 30 mg Specific characteristics of the reports are described below: Patient A, a male aged 41-50 experienced symptoms of after 2 use of esomeprazole for. The patient received monthly injections for supplementation. At the time of reporting the patient had not recovered. Patient B, a male aged 51-60 experienced a decreased blood level of after 3 use of esomeprazole for. The patient recovered. Patient C, a female aged 31-40 experienced after using esomeprazole as prophylaxis for NSAID therapy. The latency time is not reported. The patient outcome is not reported. Patient D, a female aged 51-60 experienced after 7 use of omeprazole for. The patient was treated with hydroxocobalamin injections after a level of 182 mol/l (< 175 mol/l is characteristic for a [4]). At the time of reporting the patient had not recovered. Other sources of information SmPC for esomeprazole containing products [3]. Decrease in absorption of due to hypochlorhydria is mentioned in omeprazole containing products in the special warning and precautions for use section of the SmPC [2]. Decrease in absorption of due to hypochlorhydria is mentioned in pantoprazole containing products in the special warning and precautions for use section of the SmPC [5] for lansoprazole containing products [6] for rabeprazole containing products [7] Literature A Medline search revealed some publications on the possible association between (es)omeprazole and. A few case reports [8,9], one clinical trial in patients with Zollinger-Ellison Syndrome (ZES) [10], and one rather small clinical trial in 10 healthy volunteers [11] were found. They all clearly show that omeprazole therapy, after 3 to 4, will decrease the absorption of 2

by preventing its cleavage from dietary proteins. According to the publication, ZES patients and elderly are especially at risk. The 2 case reports show the decrease in over time in a period of 4 of PPI use. The cases are supported with serum levels [8,9]. The clinical trial in patients with ZES shows that after omeprazole treatment of 4.5, levels, but not serum folate levels or any hematological parameter, were significantly lower, especially in those with omeprazole-induced sustained hyposecretion or complete achlorhydria [10]. [12] The small clinical trial shows that healthy volunteers taking widely used omeprazole doses of 1dd 20mg and 1dd 40mg led to significantly decreased absorption within 2 weeks [11]. Databases On July 20, 2011 the database of the Netherlands Pharmacovigilance Centre Lareb contained one case of decreased and 3 cases of in association with (es)omeprazole. This resulted in a reporting odds ratio (ROR) of 9.7 (95% CI: 2.8-33.9) for. The WHO database of the Uppsala Monitoring Centre contained 55 reports of and 5 reports of decreased following omeprazole administration, and contained 23 reports of and 4 reports of decreased following esomeprazole administration. The combined reporting odds ratio for esomeprazole and omeprazole with vitamin B12 and decreased was 13.4 (95% CI: 10.8-16.7). A summary of the above is shown in Table 2. Table 2. Reports of associated with (es)omeprazole in the WHO database ADR (MedDRA PT) Drug Number of reports ROR (95% CI) Vitamin B12 or Vitamine B12 decreased esomeprazole or omeprazole 87 13.4 (10.8-16.7) On August 15, 2011, the Eudravigilance database contained 21 reports of decreased levels or of, which was reported disproportionally (ROR = 4.2, 95% CI: 2.7 6.6). It concerns thirteen females and eight males. The median age of the patients was 68 (range 38 81 ). In one case, the age was not reported. A total of seventeen reports were classified as serious, three were non-serious and in one case the seriousness was not reported. Prescription data The number of patients using omeprazole and esomeprazole in the Netherlands is shown in table 4. Table 4. Number of patients using (es)omeprazole in the Netherlands between 2006 and 2010 [12]. Drug 2006 2007 2008 2009 2010 Omeprazole 895,460 1,039,000 1,220,000 1,376,000 1,604,000 Esomeprazole 256,770 284,320 310,670 344,680 342,530 3

Mechanism Vitamin B12 is a water-soluble vitamin derived from microorganisms, and the vitamin is tightly bound to dietary protein. Hydrochloric acid and pepsin release from dietary protein in the stomach where it binds to salivary R proteins. R proteins pick up and transport it through the stomach into the small intestine. R proteins are found in many fluids in the human body including saliva and stomach secretion. After alteration of R proteins by pancreatic enzymes, is rapidly transferred to intrinsic factor to form a complex that is resistant to proteolysis. Once the intrinsic factor- complex is formed in the upper jejunum, it remains intact until it adheres to specific receptors in the distal ileum. (Es)omeprazole acts by inhibiting the H + /K + ATP-ase, a proton pump that seems to be specific to the gastric parietal cells. Prolonged omeprazole treatment can result in by three possible mechanisms: 1) in hypo- or achlorhydria, protein-bound may not be adequately released from food for transfer to R protein and intrinsic factor; 2) omeprazole may decrease intrinsic factor secretion after long-term therapy even though no effect on intrinsic factor secretion occurred after a single intravenous dose of omeprazole, and 3) achlorhydria causes gastric bacterial overgrowth that may accelerate the development of by producing analogs that compete with absorption and use of the vitamin [11]. Discussion and conclusion Lareb received 4 reports concerning or decreased with the use of (es)omeprazole. In one case a positive dechallenge has been reported, in two cases the patient did not recover. One should expect more reports from omeprazole than for esomeprazole, since its longer on the market and more widely prescribed. Probably due to increased reporting shortly after introduction of a drug on the market, we received more reports from esomeprazole. Latencies were rather consistent between cases, varying from two to three with one outlier of seven. The period of seven could partially be explained by the nature of the symptoms which are often not immediately recognized and diagnosed. The latency of 2 approaches the time period after which may be expected. All together they can fit in the latency period found in literature [4]. According to the information Lareb received, the patients did not belong to the risk groups, ZES patients and elderly, associated with deficiencies as a consequence of PPI use. This indicates that this association might be more common than previously assumed. However, since there is no information on the pre existence of before starting with the proton pump inhibiter, protopathic bias cannot be excluded Of the mentioned possible mechanisms, a decrease in absorption of due to (es)omeprazole induced hypo- or achlorhydria is most plausible, most cited and already included in the SmPC of omeprazole containing products. According to this mechanism, all proton pump inhibiters share the property to decrease absorption. These cases indicate the existence of associated with the use of proton pump inhibiters. 4

References 1. College voor zorgverzekeringen. Farmacotherapeutisch Kompas. (version date: 2011, access date: 03-08-2011. 2. Dutch SmPC Losec 10/20/40mg. (version date: 2011, access date: 03-08-2011. 3. Dutch SmPC Nexium 20/40mg. (version date: 2011, access date: 03-08-2011. 4. Feenstra L; Meinders AE; Van Schil PEY, et al. Codex Medicus. 12th ed. 2011. 5. Dutch SmPC Pantozol 40 mg. (version date: 5, access date: 03-08-2011. 6. Dutch SmPC Prezal 15,30 mg. (version date: 2011, access date: 03-08-2011. 7. Dutch SmPC Pariet 10,. (version date: 2011, access date: 03-08-2011. 8. Bellou A, imone-gastin I, De Korwin JD, Bronowicki JP, Moneret-Vautrin A, Nicolas JP, Bigard MA, Gueant JL. Cobalamin with megaloblastic anaemia in one patient under long-term omeprazole therapy. J.Intern.Med. 1996;240(3):161-4. 9. Ruscin JM, Page RL, Valuck RJ. Vitamin B(12) associated with histamine(2)-receptor antagonists and a proton-pump inhibitor. Ann.Pharmacother. 2002;36(5):812-6. 10. Termanini B, Gibril F, Sutliff VE, Yu F, Venzon DJ, Jensen RT. Effect of long-term gastric acid suppressive therapy on serum levels in patients with Zollinger-Ellison syndrome. Am.J.Med. 1998;104(5):422-30. 11. Marcuard SP, Albernaz L, Khazanie PG. Omeprazole therapy causes malabsorption of cyanocobalamin (). Ann.Intern.Med. 1994;120(3):211-5. 12. College for health insurances. GIP databank. (version date: 2011, access date: 03-08-2011. This signal has been raised on November 2011. It is possible that in the meantime other information became available. For the latest information please refer to the website of the MEB www.cbgmeb.nl/cbg/en/default.htm or the responsible marketing authorization holder(s). 5