Conforms to ANSI Z400.5-2004 Standard (United States) 1. Product name Material uses Material Safety Data Sheet Product and company identification Supplier/Manufacturer Part No. (Chemical Kit) 200550 Part No. Validation date In case of emergency 2. Emergency overview Stratagene GeneMorph II Random Mutagenesis Kit, Catalog #200550 Hazards identification Stratagene GeneMorph II Random Mutagenesis Kit, Catalog #200550 Analytical reagent. Agilent Technologies, Inc. 1834 State Highway 71 West Cedar Creek, TX 78612 0.03 ml 0.15 ml 0.03 ml 0.15 ml 200550-51 200550-52 600550-53 600550-54 04/30/2010 1-800-894-1304 Physical state Liquid. Liquid. Liquid. Liquid. Odor OSHA/HCS status This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. Date of issue 04/30/2010 200550 1/15
2. Hazards identification Hazard statements Precautions Routes of entry Potential acute health effects Inhalation Ingestion Skin Eyes Potential chronic health effects MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. Avoid breathing vapor or mist. Avoid contact with eyes. Avoid prolonged or repeated contact with skin. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. Avoid prolonged contact with eyes, skin and clothing. Avoid prolonged contact with eyes, skin and clothing. Avoid prolonged contact with eyes, skin and clothing. Slightly irritating to the respiratory system. Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure. Slightly irritating to the skin. Slightly irritating to the eyes. Date of issue 04/30/2010 200550 2/15
2. Hazards identification Chronic effects Contains material that may cause target organ damage, based on animal data. Carcinogenicity Mutagenicity Teratogenicity Developmental effects Fertility effects Target organs Contains material which may cause damage to the following organs kidneys, upper respiratory tract, skin, eye, lens or cornea. Over-exposure signs/symptoms Inhalation Adverse symptoms may include the following respiratory tract irritation coughing Ingestion Skin Adverse symptoms may include the following irritation redness Eyes Adverse symptoms may include the following irritation watering redness Date of issue 04/30/2010 200550 3/15
2. Hazards identification Medical conditions aggravated by overexposure See toxicological information (section 11) 3. Composition/information on ingredients Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. None known. None known. None known. Name CAS number % Glycerol 56-81-5 15-40 There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. 4. First aid measures Eye contact Skin contact Date of issue 04/30/2010 200550 Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention if symptoms occur. Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention if symptoms occur. Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention if symptoms occur. In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention if symptoms occur. In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention if symptoms occur. In case of contact, immediately flush skin with 4/15
4. Inhalation Ingestion First aid measures plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention if symptoms occur. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms occur. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms occur. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms occur. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention if symptoms occur. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention if symptoms occur. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention if symptoms occur. Protection of first-aiders risk or without suitable training. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. risk or without suitable training. risk or without suitable training. Date of issue 04/30/2010 200550 5/15
4. First aid measures Notes to physician 5. Fire-fighting measures Flammability of the product Extinguishing media risk or without suitable training. No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours. No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. In a fire or if heated, a pressure increase will occur and the container may burst. In a fire or if heated, a pressure increase will occur and the container may burst. In a fire or if heated, a pressure increase will occur and the container may burst. In a fire or if heated, a pressure increase will occur and the container may burst. Suitable Use an extinguishing agent suitable for the surrounding fire. Use an extinguishing agent suitable for the surrounding fire. Use an extinguishing agent suitable for the surrounding fire. Use an extinguishing agent suitable for the surrounding fire. Not suitable None known. None known. None known. None known. Special exposure hazards risk or without suitable training. risk or without suitable training. risk or without suitable training. risk or without suitable training. Hazardous thermal Decomposition products may include the following decomposition products materials carbon dioxide carbon monoxide Decomposition products may include the following materials carbon dioxide carbon monoxide nitrogen oxides Date of issue 04/30/2010 200550 6/15
5. Fire-fighting measures Special protective equipment for fire-fighters 6. Accidental release measures Personal precautions Environmental precautions Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Put on appropriate personal protective equipment (see section 8). risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Put on appropriate personal protective equipment (see section 8). risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Put on appropriate personal protective equipment (see section 8). Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Date of issue 04/30/2010 200550 7/15
6. Accidental release measures Methods for cleaning up 7. Handling and storage Handling Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Date of issue 04/30/2010 200550 8/15
7. Handling and storage Storage Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. 8. Exposure controls/personal protection Ingredient Exposure limits Glycerol ACGIH TLV (United States, 1/2009). TWA 10 mg/m³ 8 hour(s). Form Mist OSHA PEL (United States, 11/2006). TWA 5 mg/m³ 8 hour(s). Form Respirable fraction TWA 15 mg/m³ 8 hour(s). Form Total dust OSHA PEL 1989 (United States, 3/1989). TWA 5 mg/m³ 8 hour(s). Form Respirable fraction TWA 10 mg/m³ 8 hour(s). Form Total dust Date of issue 04/30/2010 200550 9/15
8. Exposure controls/personal protection Recommended monitoring procedures Engineering measures Hygiene measures Personal protection Respiratory Hands Eyes Skin Environmental exposure controls Other protection 9. If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Physical and chemical properties Physical state Liquid. Liquid. Liquid. Liquid. Flash point Auto-ignition temperature Flammable limits Color Date of issue 04/30/2010 200550 10/15
9. Physical and chemical properties Odor ph 8 8.5 7.5 7.5 Boiling/condensation point Melting/freezing point Specific gravity Vapor pressure Vapor density Volatility Odor threshold Evaporation rate Viscosity Solubility Easily soluble in the following materials cold water and hot water. Easily soluble in the following materials cold water and hot water. Easily soluble in the following materials cold water and hot water. Easily soluble in the following materials cold water and hot water. Date of issue 04/30/2010 200550 11/15
10. Stability and reactivity Chemical stability Conditions to avoid Materials to avoid Hazardous decomposition products Possibility of hazardous reactions 11. The product is stable. Toxicological information Acute toxicity Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Slightly reactive or incompatible with the following materials organic materials. Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous reactions will not occur. Product/ingredient name Result Species Dose Exposure Glycerol LD50 Oral Rat 12600 mg/kg - Chronic toxicity Irritation/Corrosion Sensitizer Carcinogenicity Mutagenicity Teratogenicity Date of issue 04/30/2010 200550 12/15
11. Toxicological information Reproductive toxicity Other adverse symptoms 12. Ecotoxicity Ecological information Aquatic ecotoxicity Product/ingredient name Octanol/water partition coefficient This material is harmful to aquatic life. May cause long-term adverse effects in the aquatic environment. Result Species Mutazyme II DNA polymerase Glycerol Acute LC50 54 to 57 ml/l Fresh water Fish - Oncorhynchus mykiss - 0.9 g Other adverse effects 13. Disposal considerations Waste disposal Exposure 96 hours The generation of waste should be avoided or minimized wherever possible. Empty containers or liners may retain some product residues. This material and its container must be disposed of in a safe way. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any byproducts should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. RCRA classification Disposal should be in accordance with applicable regional, national and local laws and regulations. Local regulations may be more stringent than regional or national requirements. The information presented below only applies to the material as supplied. The identification based on characteristic(s) or listing may not apply if the material has been used or otherwise contaminated. It is the responsibility of the waste generator to determine the toxicity and physical properties of the material generated to determine the proper waste identification and disposal methods in compliance with applicable regulations. Refer to Section 7 HANDLING AND STORAGE and Section 8 EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. Date of issue 04/30/2010 200550 13/15
14. Transport information Regulatory information DOT / IMDG / IATA / 15. HCS Classification Not regulated. Regulatory information U.S. Federal regulations Clean Air Act Section 112(b) Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Substances Clean Air Act Section 602 Class II Substances DEA List I Chemicals (Precursor Chemicals) DEA List II Chemicals (Essential Chemicals) Target organ effects Not regulated. Not regulated. Not regulated. TSCA 8(a) PAIR Poly(oxy-1,2-ethanediyl),.alpha.-[(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy- TSCA 8(a) IUR Glycerol; Sorbitan monolaurate, ethoxylated; Poly(oxy-1,2-ethanediyl),.alpha.-[(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy-; Water United States inventory (TSCA 8b) At least one component is not listed. SARA 302/304/311/312 extremely hazardous substances No products were found. SARA 302/304 emergency planning and notification No products were found. SARA 302/304/311/312 hazardous chemicals Glycerol SARA 311/312 MSDS distribution - chemical inventory - hazard identification Glycerol Immediate (acute) health hazard, Delayed (chronic) health hazard Clean Water Act (CWA) 307 No products were found. Clean Water Act (CWA) 311 Edetic acid Clean Air Act (CAA) 112 accidental release prevention No products were found. Clean Air Act (CAA) 112 regulated flammable substances No products were found. Clean Air Act (CAA) 112 regulated toxic substances No products were found. Not listed Not listed Not listed Not listed Not listed State regulations Massachusetts New York New Jersey Pennsylvania California Prop. 65 No products were found. The following components are listed GLYCERINE MIST None of the components are listed. The following components are listed GLYCERIN; 1,2,3-PROPANETRIOL The following components are listed 1,2,3-PROPANETRIOL Date of issue 04/30/2010 200550 14/15
16. Other information Label requirements Date of issue Date of previous issue 04/30/2010 No previous validation. Version 1 Indicates information that has changed from previously issued version. MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. NOT EXPECTED TO PRODUCE SIGNIFICANT ADVERSE HEALTH EFFECTS WHEN THE RECOMMENDED INSTRUCTIONS FOR USE ARE FOLLOWED. Notice to reader Disclaimer The information contained in this document is based on Agilent s state of knowledge at the time of preparation. No warranty as to its accurateness, completeness or suitability for a particular purpose is expressed or implied. Date of issue 04/30/2010 200550 15/15