Contrast-Enhanced Ultrasound (CEUS)

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Contrast-Enhanced Ultrasound (CEUS) Odd Helge Gilja, MD, PhD Professor Department of Medicine Haukeland University Hospital Bergen, Norway

The Micro-Bubble

Contrast Agents - World View IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, vol. 60, no. 1, January 2013

f 0 2f 0 f 0 Microbubbles

Sonazoid Membrane is hydrogenated egg phosphatidylserine sodium (HEPSNa) The gas is perfluorobutane (PFB)

Some properties of Sonazoid Narrow Size distribution Very robust: Allow for high-mi scan (0,2-0,3) Perform a post-vascular scan Use higher frequencies Phagocytosis by Kupffer cells in the liver Yanagisawa et al.

Fundamental Imaging

Harmonic Contrast Imaging

Fine tuning the Instrument by adjusting the MI Non-linear response from microbubbles is based on two different mechanisms: non-linear response from microbubble oscillations at low acoustic pressure, chosen to minimize disruption of the microbubbles. Low MI Imaging. high energy broadband non-linear response arising from microbubble disruption.

www.efsumb.org European Federation of Societies for Ultrasound in Medicine and Biology Euroson Congresses (Next: Vienna Aug 2011) Euroson Schools since 1992, No.=58 as of Sept-10 Young Investigator Awards Guidelines and Recommendations Minimum training recommendations 9 specific disciplinary guidelines Several on safety issues Contrast agents Ultraschall in der Medizin/European Journal of Ultrasound - Issue 4-2004

New Guidelines 2008 Ultraschall/EJU 2008;29:28-44

New Guidelines for CEUS 2011 Ultraschall Med / EJU Aug. 2011

Indications - Liver Characterization of benign FLLs FNH, hemangiomas, adenomas Detection of focal lesions Metastasis Study FLL in cirrhosis Guiding of biopsies Guiding of intervention,- e.g. ablation

3 Phases in liver perfusion Arterial phase 0-30 sec. Portal phase 30-120 sec. Sinusoidal phase 2-4 min

Referred from the CT-Lab Haemangioma?

Peripheral Globular Enhancement with slow sentripetal filling

Time Intensity Curves

Hyper-echoic Tumor

CEUS Arterial Phase

CEUS Portal Phase

Lesion in Liver S7

Haemangioma High Res

Portal Phase - Sonazoid

In Haemangiomas, be aware not to use too high MI not to focus on one lesion in all phases to scan longer than 5 min. some h. never totally fill in, but it is still benign big h. may behave atypically (thrombosis)

Focal Nodular Hyperplasia - FNH FNH- a centrifugal stellate branching in early arterial phase Spoke wheel pattern in approx 40% Intense homogenous uptake Iso- or hyperechoic lesion is seen in portal venous phase. With these characteristic features: sensitivity and specificity of contrast-enhanced low MI real-time US are 87.6% and 94.5%, respectively Di Stasi 1996

FNH

FNH- Arterial Phase

FNH

FNH Late Phase (Sinosoidal)

FLL with Doppler

CEUS Sonazoid - FNH

Arterial Phase: 12 sec.

Arterial Phase: 13 sec

Arterial Phase: 13 sec

Arterial Phase: 15 sec

CEUS Flash Central scarring

Liver cell adenoma Liver cell adenoma (LCA) is a rare primary benign neoplasm found mainly in young women with a history of oral contraceptive use The hypervascularity of adenomas can be demonstrated on Doppler,- sentripetal CEUS identification of the early and homogeneous hyperechoic enhancement in the periphery of the tumor, reflecting the presence of the subcapsular feeding arteries. The enhancement of LCA in the portal and late phases is nearly comparable with that of liver parenchyma, but LCA can remain slightly hypoechoic in relation to the adjacent liver

CEUS - Real-time Perfusion Dynamic abilities outperforms CT and MR

Fatty liver Dangerous!

A common problem: Focal Lesions in Fatty Livers

CEUS in FLL

Hyperechogenic tumors in young female 20 years

CEUS Biopsy showed fatty tissue

2 Years after

Cirrhotic liver and FLL - HCC? Common clinical problem Increasing incidence world-wide AFP has limited sensitivity US (and CT) without CA has low ability to detect and characterize lesions

The Sequence of HCC Development hepatic arterial supply abnormal arterial supply Arterial supply Portal supply portal supply LRN ~ LGDN ~ HGDN ~ e-hcc ~ wdhcc ~ classical HCC early HCC from: Matsui, Clin Hep Gastro, 2005. (based on CT-arterioportal-angiography)

CEUS in AIH HCC?

CEUS in AIH HCC? Arterial Phase

FLL with B-mode and Doppler

FLL Arterial Phase

Sinusoidal Phase

CEUS in a cirrhotic liver

Late Phase

FNH versus HCC FNH HCC

2. Detection of Focal Liver Lesions Hausken et al., 1999

Liver Metastasis? Before contrast injection

Liver Metastasis? After contrast injection

Parenchymal Phase ( 4 min)

High-Frequency 9 MHz LA

Kuppfer Phase 14 min

Cholangiocarcinoma

Cholangiocarcinoma

Cholangiocarcinoma Late Phase

Lymphoma of the Liver

Late phase of Lymphoma

Safety Considerations In general, UCA are extremely safe with a low incidence of side effects They are neither nephrotoxic nor cardiotoxic The incidence of hypersensitivity or allergic events are much lower than current X-ray or MR contrast agents It is not necessary to perform laboratory tests of renal function before administering them Contraindications: Serious cardiopulmonary disease, known allergic reaction to the agent, pregnancy

Conclusion Using 2. generation ultrasound contrast agents, the method is established as an important clinical tool SonoVue is easy to apply in a clinical setting and does not prolong the exam with more than 5-10 min Attention to Mechanical Index is crucial during CEUS Benign and malignant lesions of the liver and other organs can be differentiated and detected with high sensitivity and accuracy

Yes, we scan