HOUSTON COMMUNITY COLLEGE COLEMAN COLLEGE FOR HEALTH SCIENCES BIOSAFETY TECHNICIAN PROGRAM



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HOUSTON COMMUNITY COLLEGE COLEMAN COLLEGE FOR HEALTH SCIENCES BIOSAFETY TECHNICIAN PROGRAM COURSE SYLLABUS BIOS 1471- INTRODUCTION TO LABORATORY SAFETY Freshman UTHSC-Houston UCT 1410 and 1940 - Spring 2012 UT Health Science Center at Houston University Center Tower (UCT) 7000 Fannin St. Spring Hours: 3 hour lecture/ 3 hour lab/16 weeks 96 Contact Hours Type of Instruction: Lecture and Lab Administrative Notes Primary Instructor: Dr. ROBERT (BOB) EMERY Office: UT Health Science Center Operations Center Building OCB 1.330 Office hours: By prearranged appointment only Phone: 713-500-8100 Email: Robert.J.Emery@uth.tmc.edu Course Schedule Semester: Jan. 17, 2012 May 13, 2012 Hours: Thurs Lecture 9:00 12:00 pm Rm. UCT 1410 Thurs Lab 1:00 4:00 pm Rm. UCT 1940 Course Description Topics include safe handling of biological, chemical, radiation, and nano scale materials. Focuses on safety regulations and recommendations, and proper materials handling in research, clinical and petrochemical laboratories. Covers the classification levels of laboratories (i.e., Biosafety Level 1, 2, 3 and 4 requirements), laboratory risk assessment, medical surveillance requirements as part of an occupational health program, routine safety surveillance activities, identification of appropriate decontamination methods for biological, radiological, chemical or nano particle accidents, spills, and training materials required to educate personnel in all areas of laboratory safety, including biological safety, chemical safety, radiation safety, and recombinant DNA research activities. Course Prerequisite BIOL 1406 and CHEM 1411 and MATH 1314 BIOS 1470 FREQUENT REQUISITES College Level Reading

College Level Mathematics College Level Writing Course Goals 1. Integrate ethical and professional behavior in the research, biotechnology, pharmaceutical or other working or learning environments. 2. Use problem-solving skills to integrate safety and research practices in a real life settings. 3. Demonstrate working knowledge of safety and applicable regulations and guidance necessary to be a successful Biosafety Technician. 4. Ability and knowledge of safe laboratory techniques and waste disposal. Program Learning Outcomes 1. Use problem-solving skills to integrate safety and research practices in real life settings. 2. Demonstrate working knowledge of safety and applicable regulations and guidance necessary to be a successful Biosafety Technician. 3. Integrate ethical and professional behavior in the research, biotechnology, pharmaceutical or other work based learning environment. 4. Safely apply techniques and instrumentation in biosafety monitoring, biohazardous waste disposal and risk management. Course Student Learning Outcomes 1. Demonstrate ethical and professional behavior. 2. Perform safe laboratory practices and waste disposal. 3. Identify and use applicable safety guidance documents and regulations. 4. Apply principles of safety, quality assurance and risk management in the laboratory setting. Learning Objectives 1.1 Demonstrate respect and appropriate interpersonal skills with instructors and other students. 1.2 Illustrate ethical and professional behavior by adhering to attendance policies, dress code, and general rules and regulations. Perform safety work skills. 1. Following instruction, demonstration and practice sessions, identify the basic concepts of laboratory risk identification, occupational health, safety surveillance in the work areas as demonstrated in through practical exams. 2. Apply safe handling of biological, chemical, radiation and nano scale materials. Guidance Documents/Regulations 1. Demonstrate working knowledge of safety regulations and guidance documents in conjunction with proper materials handling needed in research, clinical and petrochemical laboratories by scoring 70% or better on practical exams. Psychomotor 1. Given appropriate instruction and all necessary supplies and equipment, the student will perform the following tasks and demonstrate mastery of each task as determined by the instructor and common standards of practice.

Apply principles of safety, quality assurance and risk management in the biotechnology and other laboratory environments. 1. Demonstrate the applications of biotechnology with a biosafety emphasis and integrate these concepts into the research and clinical laboratory environment. 2. Evaluate biotechnology applications and the required safety procedures that must be followed in the laboratory environment. Behavioral Upon receiving appropriate instructions, the student will demonstrate the following attitudes and behaviors at all times as determined by mid-term and end-term evaluations. During the course of the semester, the students will: 1. attentively attend to verbal and demonstrative instruction 2. follow written and verbal instructions 3. communicate effectively in written and spoken English 4. engage in class/laboratory discussions by asking pertinent questions and responding respectfully to other student s comments 5. demonstrate a willingness to learn and apply new ideas/technical skills to future endeavors 6. demonstrate a positive teamwork ethic by being willing to assist and cooperate with others 7. develop confidence by gradually working independently in a competent manner 8. prioritize and manage work flow within a restricted time frame 9. handle themselves at all times in a professional manner and perform at the highest level of standards 10. demonstrate honesty and integrity 11. demonstrate commitment to the Biosafety procedures and applications 12. be punctual to class and do not abuse break times. Scans, Competencies, and Foundations Houston Community College is determined to prepare you with the knowledge and skills you need to succeed in the work place. The following competencies and foundation skills have been designed into the curriculum of the course to accomplish this. Demonstrate ethical and professional behavior. Perform safety and biotechnology work skills. Identify the regulations and guidance documents relevant to safety. Apply principles of safety, quality assurance and risk management in the biotechnology and other laboratory environments. Jan 19 Week 1: Introduction to Safety Standards and History of Safety Laboratory Week 1: Laboratory rules and site visit tour rules Jan 26 Week 2: Biosafety in Microbiological and Biomedical Laboratories (BMBL) and Bloodborne Pathogens Training Laboratory Week 2: Risk group classification exercises

Feb 2 Week 3: NIH rdna Guidelines and Institutional Biosafety Committee Laboratory Week 3: rdna experiment classification exercises Feb 9 Week 4: Biological Safety Cabinets, NSF49 Laboratory Week 4: Biological safety cabinet certification Feb 16 Week 5: Exam Laboratory Week 5: Select Agents and Bioterrorism Feb 23 Week 6: Laboratory Standard and Hazard Communication Laboratory Week 6: Material Data Safety Sheets/ Chemical Segregation & Storage exercise Mar 1 Week 7: Personal Protective Equipment (PPE) Laboratory 7: Donning and doffing of PPE Mar 8 Week 8: Respiratory Protection Laboratory Week 8: Qualitative and quantative respirator fit testing Mar 15 SPRING BREAK Mar 22 Week 9: Exam Laboratory Week 9: Radiation Safety, Radiation safety survey, Geiger-Muller meter use Mar 29 Week 10: Safety and Laboratory Animal Use Laboratory Week 10: Tour of UTHealth animal facilities Apr 5 Week 11: Occupational Health Laboratory Week 11: Tour of UTHealth Employee Health Clinic Apr 12 Week 12: Fire Safety, NFPA45 Laboratory Week 12: Fire extinguisher training Apr 19 Week 13: Exam Laboratory Week 13: Biological waste and autoclave training

Apr 26 Week 14: Radiation and Chemical waste Laboratory Week 14: Tour of waste processing facility at UTHealth May 3 Week 15: Environmental releases Laboratory Week 15: Spill drill exercise May 10 Week 16: Exam Laboratory Week 16: Measuring Safety Program Performance and Professional Development Instructional Methods Instructional strategies will include classroom lectures, guest speakers and demonstrations, hands-on practice sessions, case studies, computer-generated instructional programs, and internet access materials. Student Assignments Students should refer to the Course Outline and Course Calendar. Student Assessments Assessments will include hands-on lab assignments with corresponding questions concerning the pertinent chapters and lecture material covered in the labs. Refer to Course Requirements, Grading Scale, and Grading Criteria for more information. Instructional Materials Students completing the prerequisite BIOS 1470 will already have the required textbook for that course: DNA Science: A First Course (2 nd edition). By Micklos, DA, Freyer GA and Crotty DA. Cold Pring Harper Laboratory Press, 2003 (ISBN 0-87969-636-2). In addition, students will be required to either download or purchase the CDC/NIH publication Biosafety in the Microbiology and Biomedical Laboratory. This document can be downloaded or ordered at http://www.cdc.gov/biosafety/publications/bmbl5/. Other web based materials will be identified for use during the course. BIOS books are also sold at the West Loop Bookstore; the URL is: http://hccs.bkstore.com or http://hccs.bncollege.com. Students can order HCC textbooks online and also reserve their textbooks online for in-store pickup at the HCC West Loop bookstore. HCC West Loop bookstore phone number is 713-218-0391. No single book covers the entire scope of this course. Therefore, the student will also have to rely on course notes and other materials provided by the instructors. The HCC Coleman College library is the Houston Academy of Medicine Texas Medical Center library. It is located one mile and one METRO rail stop away at 1133 John Freeman Boulevard at Cullen Circle. Numerous reference books are available in the HAM-TMC Library and in faculty offices at Coleman College for Health Sciences. The HAM-TMC main phone

number is 713 795 4200. Library hours are Monday through Thursday 7 a.m. - 10 p.m., Friday 7 a.m. - 9 p.m., Saturday 9 a.m. - 5 p.m., and Sunday 1 p.m. - 8 p.m. Parking is available underneath the Library in Garage 3 or Garage 4 and costs approximately $12.00 maximum per day. For more information, go to www.library.tmc.edu. Circulation Privileges: Present your student ID, current registration invoice, and registration form. The registration form can be downloaded at http://resource.library.tmc.edu/circ/docs/memberregisform.pdf Remote TMC Educational Access: Go to http://resource.library.tmc.edu/resources/ Required Reading Material Occupational Health and Safety Administration (OSHA) Preamble: http://www.osha.gov/dte/grant_materials/fy07/sh-16625-07/cshandout.pdf Biosafety in the Microbiology and Biomedical Laboratory: http://www.cdc.gov/biosafety/publications/bmbl5/ Occupational Health and Safety Administration (OSHA) Bloodborne Pathogens Standard: http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=standards&p_id=10 051 Texas Administrative Code 30: http://www.tceq.texas.gov/permitting/air/rules/state Laboratory Standard: http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=standards&p_id=10 106 Radiation Protection Program: Disability Notification Any student with a documented disability (e.g. physical, learning, psychiatric, vision, hearing, etc.) who needs to arrange reasonable accommodations must contact the Disability Services Office at their respective college at the beginning of each semester. Faculty is authorized to provide only the accommodations requested by the Disability Support Services Office. If you have any questions, please contact the Disability Counselor at your college or the District Disability Office at 713-718-5165. Contact Dr.Raj Gupta, Coleman College ADA counselor, at 713-718-7631. Academic Honesty Plagiarism, cheating, and other forms of academic dishonesty are not only violations of the college system and the rules of this class, but are unethical and unprofessional. Students engaging in any form of academic dishonesty are subject to immediate dismissal from the program. You are expected to be familiar with the College's Policy on Academic Honesty, found in the catalog and student handbook. Students are responsible for conducting themselves with honor and integrity in fulfilling course requirements. Penalties and/or disciplinary proceedings may be initiated by College System officials against a student accused of scholastic dishonesty. Scholastic dishonesty : includes, but is not limited to, cheating on a test, plagiarism, and collusion. Cheating on a test includes: Copying from another students test paper; Using materials not authorized by the person giving the test; Collaborating with another student during a test without authorization;

Knowingly using, buying, selling, stealing, transporting, or soliciting in whole or part the contents of a test that has not been administered; Bribing another person to obtain a test that is to be administered. Plagiarism means the appropriation of another s work and the unacknowledged incorporation of that work in one s own written work offered for credit. Collusion means the unauthorized collaboration with another person in preparing written work offered for credit. Possible punishments for academic dishonesty may include a grade of 0 or F in the particular assignment, failure in the course, and/or recommendation for probation or dismissal from the College System. (See the Student Handbook). Student Attendance, Repeat Course Fee, Withdrawals Students will be dropped from any BIOS course for excessive absences. Absences in excess of 12.5% of the hours of instruction are considered excessive. Students will be dropped from any BIOS course for excessive tardiness. Ten minutes late for class will be considered tardy. Three tardies will count as one absence. Leaving class early without prior notification will be considered as absent time and will be noted. Students are advised to communicate with the instructor about absences and tardies. Call to inform the instructor of unexpected absences or tardies. If a student knows in advance that they will be late or absent, they should notify the instructor in writing one week in advance. Excused absences may be given if the student notifies the instructor in advance and if the student provides appropriate documentation to explain the absence. Students are responsible for material missed because of absences. It is the student's responsibility to schedule all makeup work. In the event of bad weather, the student is advised to listen to local radio or television stations for information about school closure. Repeat Course Fee The State of Texas encourages students to complete college without having to repeat failed classes. To increase student success, students who repeat the same course more than twice, are required to pay extra tuition. The purpose of this extra tuition fee is to encourage students to pass their courses and to graduate. Effective fall 2006, HCC will charge a higher tuition rate to students registering the third or subsequent time for a course. If you are considering course withdrawal because you are not earning passing grades, confer with your instructor/counselor as early as possible about your study habits, reading and writing homework, test taking skills, attendance, course participation, and opportunities for tutoring or other assistance that might be available. Withdrawals Students are responsible for officially withdrawing from classes. The last day to drop with a W is January 18, 2012. Students who fail to withdraw from a class before this date will receive a grade of F. Before you withdraw from your course, please take the time to meet with the instructor to discuss why you feel it is necessary to do so. The instructor may be able to provide you with suggestions that would enable you to complete the course. Your success is very important.

To help you avoid having to drop/withdraw from any class, contact your professor regarding your academic performance. You may also want to contact your counselor to learn about helpful HCC resources (e.g. online tutoring, child care, financial aid, job placement, etc.). HCC has instituted an Early Alert process by which your professor may alert you and the counselors that you might fail a class because of excessive absences and/or poor academic performance. Students should check HCC s Academic Calendar by Term for drop/withdrawal dates and deadlines. Student may also check the course syllabus for the withdrawal date. If a student decides to drop or withdraw from a class upon careful review of other options, the student can drop online prior to the deadline through their HCC Student Service Center: https://hccsaweb.hccs.edu:8080/psp/csprd/?cmd=login&languagecd=eng Course Withdrawals-First Time Freshmen Students-Fall 2007 and Later Under Section 51.907 of the Texas Education Code an institution of higher education may not permit a student to drop more than six courses, including any course a transfer student has dropped at another institution of higher education. Beginning in fall 2007, the Texas Legislature passed a law limiting first time entering freshmen to no more than SIX total course withdrawals throughout their educational career in obtaining a certificate and/or degree. Classroom Behavior Turn cell phones off and pagers to vibrate mode during class. Attend to all personal business before the start of class. Students will not be allowed to leave the classroom during a test. Do not work on assignments or other course work during class. Conflicts should be brought to the attention of the instructor as soon as possible. HCC Student Services Information Early alert: HCC has instituted an Early Alert process by which your professor will alert you through written contact actions and through counselors of concerns that you might fail a class because of excessive absences and/or poor academic performance. It is your responsibility to visit with your professor or a counselor to learn about what, if any, HCC interventions might be available to assist you online tutoring, child care, financial aid, job placement, etc. to stay in class and improve your academic performance. Instructor Requirements As your instructors, it is our responsibility to: Provide the grading scale and detailed grading formula explain how student grades are to be derived Facilitate an effective learning environment through class labs, lectures, presentations, reviews, and other materials Description of any special projects or assignments Inform students of policies such as attendance, withdrawal, tardiness, and make up Provide the course outline and class calendar which will include a description of any

special projects or assignments The instructors will be available via blackboard and e-mail. Office hours will be announced. If the student can not make office hours, a discussion over Skype or ichat are also options. Student s Responsibilities Read lecture material before class, define unknown terms and come prepared to ask questions Attend all classes, pay close attention to instructions given by the instructor, follow procedures and participate to the fullest extent Immediately after the lecture, review lecture material covered and answer learning objectives Students should not study the night before the exam. Rather, plan to study a certain amount each day to achieve academic success Program/Discipline Requirements The Program prepares individuals, under the supervision of laboratory scientists, to perform routine laboratory procedures and safety measures and to apply preset strategies to record and analyze data. Includes instruction in general laboratory procedures and skills; laboratory mathematics; interpersonal and communications skills; and the basic principles biotechnology and applicable safety procedures. Course Requirements, Grading Scale, and Grading Criteria BIOS 1471 is a three-hour lecture, three-hour lab course. Students will be graded according the following: Quarterly Exams 20% Mid-Term Exam 20% Lab Notebooks 15% Assigned Safety Report 15% Final Exam 30% There will be four exams for this course covering both the lecture and the laboratory materials. Questions for these exams will come from the textbook, lectures and handouts given to students during lecture and lab. A comprehensive knowledge of the material presented will ensure adequate performance on exams. Students must maintain a 70% average on quarterly and mid-term exams. Students not maintaining a 70% average will receive a grade of "F" for the course. No makeup exams are given for unexcused absences. An absence on test day will result in a grade of "0". If a student must be absent for a test, the student is responsible for informing the instructor in advance and providing the instructor with appropriate documentation to explain the absence.

Lab notebooks are required for this course. A lab notebook will be provided. Lab notebooks must be kept current and will be reviewed by the instructor during each lab period. Help will be given to assist students in being successful with their lab notebook. Students not scoring a 70% or better on their notebook will receive a grade of F for the course. The lab notebook should consist of the following elements for each laboratory: The days date Exercise Title Observations Conclusions The safety report will consist of the creation of a document that describes a safety assessment that would be suitable for review by a regulatory agency. Typically 2-3 pages in length, double-spaced using a standard font (e.g., Arial) and a size of 12 point. The report has to contain the following basic elements: 1- Title; 2-Background; 3- Main Text describing assessment performed, 4- Discussion and at least 3 references in a Bibliography. Papers must be typed. The paper will be due at the start of lecture on week 12. Papers that are late will be reduced in point value 10 points per day. The mid-term and final exams are both comprehensive. The final exams (lecture and laboratory) count for 30% of the total course grade. The student must score a 70% or better for both the lecture and laboratory sections in order to pass this course. Students failing the final exam will not be allowed to retest. The following grading scale will be used for BIOS 1471: 90-100 = A 80-89 = B 70-79 = C 0-69 = F Study Strategies for Students It is highly recommended that students attend all lecture and laboratory sessions, pay close attention to instructions given by the instructor, follow procedures, and participate to the fullest extent. Students should not wait until the night before an exam to study. Studies have shown that students who study a certain amount each day are more likely to be successful. It is recommended that students read lecture material before a lecture is given, define unknown terms and prepare questions to ask the instructor during the lecture. Immediately after a lecture, the student should reread the lecture material and answer learning objectives as if they were study questions. Students are encouraged to form study groups to help in understanding the material. Activate your HCC student email account if you have not already done so. Go to http://webmail.hccs.edu after you have registered for classes. Username will be firstname.lastname with Password being DOB date of birth (mm/dd/yyyy for example

09/15/1985). If an error occurs, you may have number after your last name in the data system. To find your email username, press students click here for help link that will take you to a tutorial page. On second bullet line, press click here that opens a search engine to look up your email student identification. If you change your password, write down. THIS SYLLABUS IS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE.