Real-Time Monitoring Buoy in Mystic River Watershed Access to the data has been through a password protected site http://www.ysieconet.com/ Project Assistance: Liz McCarthy Laura Senefeld Matt Destino Tim Bridges Dick Siscanaw
Charles and Mystic River Real-Time Monitoring EPA New England Regional Laboratory The Real-Time Monitoring Buoys are off-line for the season Purpose: Environmental Protection Agency (EPA) has established monitoring buoys in the Charles and Mystic Watersheds. These buoys collect and transmit water quality data that is available to the public. EPA has established these buoys to help with the tracking of cyanobacteria blooms and water quality conditions. Note: All water quality measurements are collected 1 meter below the water's surface Last Sonde Verification: 9/3/21 Click here Project Partners: Disclaimer: The data presented on this website is considered preliminary data and may be subject to future revision or qualifiers. The data from this site is transmitted directly from the instrument with no or little review. Inaccuracies may be presented because instrument malfunction or physical changes at buoy location.
Wedge Pond Sonde Measurements Recorded every 15 minutes Measurement period 6/15-9/3/1 Measurements collected at 1 meter depth Parameters Temperature, Conductivity ph Dissolved oxygen Chlorophyll Phycocyanin
Buoy chlorophyll and phycocyanin correlated with Lab data Chlorophyll a EPA Contract lab DPH contract lab (Charles only) Cyanobacteria cell counts EPA Contract lab DPH contract lab (Charles only) Roger Frymire
Cells/mL Wedge Pond Phycocyanin Concentrations 4 35 3 25 2 15 1 5 6/15/1 6/22/1 6/29/1 7/6/1 7/13/1 7/2/1 7/27/1 8/3/1 8/1/1 8/17/1 8/24/1 8/31/1 9/7/1 9/14/1 9/21/1 9/28/1 Date
6/15/1 6/22/1 6/29/1 7/6/1 7/13/1 7/2/1 7/27/1 8/3/1 8/1/1 8/17/1 8/24/1 8/31/1 9/7/1 9/14/1 9/21/1 9/28/1 Cells/mL Wedge Pond Phycocyanin Concentrations 25 2 15 1 5 Date
(mg/l) Wedge Pond Dissolved Oxygen 17 15 13 11 9 7 5 3 6/15/1 6/22/1 6/29/1 7/6/1 7/13/1 7/2/1 7/27/1 8/3/1 8/1/1 8/17/1 8/24/1 8/31/1 9/7/1 9/14/1 9/21/1 9/28/1
Wedge Pond ph Measurements 1 9.5 9 8.5 8 7.5 7 6.5 6 6/15/1 6/22/1 6/29/1 7/6/1 7/13/1 7/2/1 7/27/1 8/3/1 8/1/1 8/17/1 8/24/1 8/31/1 9/7/1 9/14/1 9/21/1 9/28/1
5/27/1 6/3/1 6/1/1 6/17/1 6/24/1 7/1/1 7/8/1 7/15/1 7/22/1 7/29/1 8/5/1 8/12/1 8/19/1 8/26/1 9/2/1 9/9/1 9/16/1 9/23/1 9/3/1 Phycocyanin (cells/ml) 6/15/1 6/22/1 6/29/1 7/6/1 7/13/1 7/2/1 7/27/1 8/3/1 8/1/1 8/17/1 8/24/1 8/31/1 9/7/1 9/14/1 9/21/1 9/28/1 Phycocyanin (cells/ml) Wedge Pond Phycocyanin Concentrations 5 45 4 35 3 25 2 15 1 5 Date Chalres RTM Buoy Phycocyanin Concentrations 5 45 4 35 3 25 2 15 1 5 Date
Summary of Use and Results Evaluation user feedback was positive Most interest in tracking cyanobacteria blooms Relocating Mystic Buoy Good probe performance against calibration standards Chlorophyll probe correlated poorly with laboratory Chlorophyll a method Phycocyanin correlated good with DPH Lab and Roger Frymire but poorly with EPA contract lab.
Next steps for RTM Buoy Project Finalize and report out the validated data Plan on deploying again in the Mystic and Charles next summer Find a new location for the Mystic Buoy Investigate different calibration methods for chlorophyll and phycocyanin Cultured cells for phycocyanin Investigate Public website
Questions?
DO (mg/l) Charles RTM Buoy Dissolved Oxygen Concentrations 16 14 12 1 8 6 4 2 5/27/1 6/3/1 6/1/1 6/17/1 6/24/1 7/1/1 7/8/1 7/15/1 7/22/1 7/29/1 8/5/1 Date 8/12/1 8/19/1 8/26/1 9/2/1 9/9/1 9/16/1 9/23/1 9/3/1
5/27/1 6/3/1 6/1/1 6/17/1 6/24/1 7/1/1 7/8/1 7/15/1 7/22/1 7/29/1 8/5/1 8/12/1 8/19/1 8/26/1 9/2/1 9/9/1 9/16/1 9/23/1 9/3/1 ph Charles RTM Buoy ph 1 9.5 9 8.5 8 7.5 7 6.5 6 Date
5/27/1 6/3/1 6/1/1 6/17/1 6/24/1 7/1/1 7/8/1 7/15/1 7/22/1 7/29/1 8/5/1 8/12/1 8/19/1 8/26/1 9/2/1 9/9/1 9/16/1 9/23/1 9/3/1 Phycocyanin (cells/ml) 35 Charles RTM Buoy Phycocyanin Corrected (w/dph values) Concentrations 3 25 RTM Buoy Corrected DPH cell counts 2 15 1 5 Date
Phycocyanin (cells/ml) Charles RTM Buoy Daily Max Phycocyanin corrected (DPH) values 35 3 25 72 days above 7, 55 days equal to or below 7, 2 15 1 5 5/27/1 6/3/1 6/1/1 6/17/1 6/24/1 7/1/1 7/8/1 7/15/1 7/22/1 7/29/1 Date 8/5/1 8/12/1 8/19/1 8/26/1 9/2/1 9/9/1 9/16/1 9/23/1 9/3/1
Phycocyanin probe (cells/ml) Phycocyanin probe (cells/ml) Phycocyanin probe (cells/ml) Phycocyanin probe (cells/ml) Phycocyanin probe correlation with Laboratory Cyanobacteria cell counts 14 12 1 8 6 4 2 LSSU (EPA contract lab) cyanobacteria cell counts Vs Phycocyanin Probe With two outlier removed (Charles and Mystic data) y =.353x + 2752.8 R 2 =.4679 5 1 15 2 25 3-2 LSSU cell counts (cells/ml) 4 35 3 25 2 15 1 5 Roger Frymirecyanobacteria cell counts Vs Phycocyanin Probe (Charles and Mystic data) y =.69x + 244.5 R 2 =.983 1 2 3 4 5 6 RF cell counts (cells/ml) 4, 35, 3, 25, 2, 15, 1, 5, DPH contract lab cyanobacteria cell counts Vs. Phycocyanin probe Charles River data y =.1364x + 2463.9 R 2 =.7417 5, 1, 15, 2, 25, 3, Total (DPH) Cell Count 1 9 8 7 6 5 4 3 2 1 Roger Frymire cyanobacteria cell counts Vs Phycocyanin Probe With high point removed (Charles and Mystic data) y =.555x + 2642 R 2 =.7539 2 4 6 8 1 12 RF cell counts (cells/ml) Note: DPH samples collected ~35 feet away at dock 6 deep
Probe Chlorophyl (ug/l) Chlorophyll probe (ug/l) Chlorophyll probe had poor correlation with Laboratory data 5 45 4 Chlorophyll a (EPA contract Lab) Vs. Chlorophyll Probe (with one outlier and data not meeting QC removed) Charles and Mystic data 35 3 25 2 15 1 5 y =.3243x + 3.2892 R 2 =.2182 1 2 3 4 5 6 7 Lab Chlorophyll a (ug/l) 4 Chlorophyll a (DPH contract lab) Vs. Chlorphyll probe Charles River data 35 3 25 2 y =.341x + 12.98 R 2 =.3 15 1 5 5 1 15 2 25 3 35 4 45 DPH Chlorophyll a values (ug/l)
RTM BUOY water Temperature (C) RTM Buoy Turbidity (NTU) RTM Buoy Sp. Cond. (us/cm) RTM Buoy Optical DO (mg/l) RTM Buoy ph Other parameter comparisons with DPH and RTM Buoy DPH Dissolved Oxygen Vs. Optical Dissolved Oxygen Probe DPH ph Vs. ph Probe 16 14 12 1 8 6 4 2 y =.3242x + 6.578 R 2 =.1519 5 6 7 8 9 1 11 12 DPH Dissolved Oxygen (mg/l) 1 y = 1.339x - 3.2119 9.5 R 2 =.851 9 8.5 8 7.5 7 7.5 8 8.5 9 9.5 1 DPH ph DPH Water Temperature Vs. Probe Water Temperature DPH Conductivity Vs. Probe Conductivity DPH Turbidity Vs. Probe Turbidity 32 7 3 3 25 6 28 2 5 26 24 y = 1.36x -.264 4 R 2 =.9792 3 15 1 y =.8791x + 321.35 R 2 =.9123 22 2 5 y =.2436x + 2.7786 R 2 =.847 2 1 22 23 24 25 26 27 28 29 3 31 5 1 15 2 25 3 DPH Water Temperature (C) 1 2 3 4 5 DPH Conductivity (us/cm) 6 7 DPH Turbidity (NTU)