Massimo Puoti Dept. of Infectious Diseases AO Ospedale Niguarda Cà Granda



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Massimo Puoti Dept. of Infectious Diseases AO Ospedale Niguarda Cà Granda Treatment of Hepatocellular carcinoma: The 2nd World Congress on Controversies in the Management of Viral Hepatitis (C-Hep) Berlin, Germany October 18-20, 2012 state of the art

Treatment of Hepatocellular Carcinoma: state of the art EASL EORTC & AASLD guidelines Critical areas in clinical decision making on HCC

Treatment of Hepatocellular Carcinoma: state of the art EASL EORTC & AASLD guidelines Critical areas in clinical decision making on HCC

Updated BCLC staging system and treatment strategy HCC Stage 0 PST 0, Child Pugh A Stage A C PST 0 2, Child Pugh A B Stage D PST > 2, Child Pugh C Very early stage (0) 1 HCC < 2 cm Carcinoma in situ Early stage (A) 1 HCC or 3 nodules < 3 cm, PST 0 Intermediate stage (B) Multinodular, PST 0 Advanced stage (C) Portal invasion, N1, M1, PST 1 2 End stage (D) 1 HCC Portal pressure(plt & Splenomegaly)/ bilirubin Increased 3 nodules 3 cm Associated diseases Normal No Yes Resection Liver transplantation Curative treatments (30%) 5-year survival (40 70%) PEI/RFA TACE Target: 20% OS: 20 mo (45-14) Sorafenib Target: 40% OS: 11 mo (6-14) Best supportive care Target: 10% OS: <3 mo EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma. Journal of Hepatology 2012 vol. 56 j 908 943

EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma Journal of Hepatology 2012 vol. 56 j 908 943 Refinement of BCLC classification BCLC B & C stage patients [Huitzil-Melendez, 2010] Large range of survival B (45-11 mo) and C (11-5 months) is quite large Large range of responses to a given treatment Further stratification of patients within each class should be explored Liver function (Child Pugh A versus B, or ascites) Prognostic variables (ECOG, cancer invasiveness) Prognostic molecular biomarkers

EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma Journal of Hepatology 2012 vol. 56 j 908 943 Molecular classification of HCC (1) to understand biological subclasses and drivers of the disease, to optimize benefits from molecular therapies to enrich trial populations [breast cancer: Her2/neu status discriminates outcome and treatment response to trastuzumab; Slamon 2001] [EGFR mutational status in NSCLC identifies the responders to TKIs; Tsao 2005] [melanoma patients with BRAF mutations respond to B-RAF inhibitors; Flaherty 2010] In HCC, no molecular subclass has been reported as responding to specific targeted therapy

Hepatocellular Carcinoma ( signaling pathways and nuclear effectors ) PROLIFERATION / SURVIVAL EGF IGF MET Akt/mTOR Rafs/Raf/MAPKs Hippo (YAP, Mst1/2, Lats1/2) INFLAMMATION IFN IL6 JAK / STATs CELL DIFFERENTIATION / EMT WNT TGF-b Sonic/Hedgehog Notch ANGIOGENESIS VEGF FGF PDGF mirnas NUCLEAR EFFECTORS p53 family -catenin E2Fs c-jun CREB / ATFs NFkBs STATs Gli 1 / 2 HIF-1 SMADs

EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma Journal of Hepatology 2012 vol. 56 j 908 943 Molecular classification of HCC (4) Molecular classification of HCC based on gene signatures or molecular abnormalities is not ready for clinical application (evidence 2A; recommendation 1B)

Conventional RECIST: longest overall tumor diameter mrecist: longest viable tumor diameter Lencioni R, Llovet JM. Semin Liver Dis. 2010;30:52 60.

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular classification of HCC based on gene signatures or molecular abnormalities is not ready for clinical application

Treatment of Hepatocellular Carcinoma: state of the art EASL EORTC & AASLD guidelines Critical areas in clinical decision making on HCC

Critical area in clinical decision making for HCC AASLD PRACTICE GUIDELINE 2010 MANAGEMENT OF HEPATOCELLULAR CARCINOMA: AN UPDATE Jordi Bruix and Morris Sherman EASL EORTC CLINICAL PRACTICE GUIDELINES 2012 MANAGEMENT OF HEPATOCELLULAR CARCINOMA Chairmen: Josep M. Llovet (EASL); Michel Ducreux (EORTC). Clinical Practice Guidelines Members: Riccardo Lencioni; Adrian M. Di Bisceglie; Peter R. Galle; Jean Francois Dufour; Tim F. Greten; Eric Raymond; Tania Roskams; Thierry De Baere; Michel Ducreux; and Vincenzo Mazzaferro. EASL Governing Board Representatives: Mauro Bernardi Reviewers: Jordi Bruix; Massimo Colombo; Andrew Zhu PRACTICAL RECOMMENDATIONS FOR THE MULTIDISCIPLINARY APPROACH TO THE TREATMENT OF HEPATOCELLULAR CARCINOMA 2012 The Italian Association for the Study of the Liver (A.I.S.F.) AISF Expert panel: Luigi Bolondi, Matteo Cescon, Umberto Cillo, Massimo Colombo, Antonio Craxì, Fabio Farinati, Edoardo Giovanni Giannini, Rita Golfieri, Massimo Levrero, Fabio Piscaglia, Giovanni Raimondo, Franco Trevisani AISF Coordinating Committee: Paolo Caraceni, Barbara Coco, Alessia Ciancio, Mirella Fraquelli, Maria Rendina, Giovanni Squadrito

Updated BCLC staging system and treatment strategy HCC Stage 0 PST 0, Child Pugh A Stage A C PST 0 2, Child Pugh A B Stage D PST > 2, Child Pugh C Very early stage (0) 1 HCC < 2 cm Carcinoma in situ Early stage (A) 1 HCC or 3 nodules < 3 cm, PST 0 Intermediate stage (B) Multinodular, PST 0 Advanced stage (C) Portal invasion, N1, M1, PST 1 2 End stage (D) 1 HCC Portal pressure(plt & Splenomegaly)/ bilirubin Increased 3 nodules 3 cm Associated diseases PST 0-1 LT? Normal No Yes Resection Liver transplantation Curative treatments (30%) 5-year survival (40 70%) PEI/RFA TACE Target: 20% OS: 20 mo (45-14) Sorafenib Target: 40% OS: 11 mo (6-14) Best supportive care Target: 10% OS: <3 mo One stage one treatment: combination treatment? Second line treatment? EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma. Journal of Hepatology 2012 vol. 56 j 908 943

AISF PRACTICAL RECOMMENDATIONS Multidisciplinary approach to HCC treatment Dig Liver Dis 2012 (in press) Critical area in clinical decision making for HCC Staging BCLC staging system limitations (1) univocal treatment option for each stage absence of indications regarding second-line or combined/sequential treatments assignment to the advanced stage (BCLC C) of all patients with a PS 1 assignment to the terminal stage (BCLC D) of patients with small tumors in Child-Pugh class C, whereas they most benefit from LT [Berry, 2012; Cillo, 2010; Vitale 2011; Merani, 2011]

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular of HCC based on gene signatures or molecular abnormalities is not ready for clinical application BCLC staging might have some limitation ( Usage of PST in cirrhotic patients, LT in CP stage C, consideration for 2 line & combination treatment)

Updated BCLC staging system and treatment strategy HCC Stage 0 PST 0, Child Pugh A Stage A C PST 0 2, Child Pugh A B Stage D PST > 2, Child Pugh C Very early stage (0) 1 HCC < 2 cm Carcinoma in situ Early stage (A) 1 HCC or 3 nodules < 3 cm, PST 0 Intermediate stage (B) Multinodular, PST 0 Advanced stage (C) Portal invasion, N1, M1, PST 1 2 End stage (D) 1 HCC Portal pressure(plt & Splenomegaly)/ bilirubin Increased 3 nodules 3 cm Associated diseases PST 0-1 Normal No Yes Resection Liver transplantation Curative treatments (30%) 5-year survival (40 70%) PEI/RFA TACE Target: 20% OS: 20 mo (45-14) Sorafenib Target: 40% OS: 11 mo (6-14) Best supportive care Target: 10% OS: <3 mo EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma. Journal of Hepatology 2012 vol. 56 j 908 943

Results of RFand resection in patients with very early HCC (single <2 cm) Propensity analysis (resection 52 vs RF 91 52) One-to-one near-neighbor matching for: sex, age, HBsAg, anti-hcv, platelet, Child-Pugh, AFP, ALT, BMI, hypertension, diabetes. Disease free survival RF has: Negligible mortality Lower liver mutilation Lower costs Shorter hospital stay Easy repeatibility Observed survival Wang JH et al., J Hepatol 2011 [Epub haed of print]

In compensated cirrhosis, first-line RF (followed by resection if failure) is better than resection (followed by RF for recurrence) if: > > < > > Cho YK et al., Hepatology 2010; 51: 1284-90

AISF PRACTICAL RECOMMENDATIONS Multidisciplinary approach to HCC treatment Dig Liver Dis 2012 (in press) Critical area in clinical decision making for HCC Treatment Ablation techniques For HCC 2 cm, RFTA = first-line treatment? similar survival rates, lower morbidity and mortality, shorter hospital stay, and lower sanitary costs) For HCC of 2.1-3 cm, choice between surgery and on a case-by-case multi-disciplinary evaluation [ rescue resection after of incomplete HCC necrosis with RFTA offers a survival chance equivalent to that of patients treated with surgery as first-line approach [Cho, 2010] In patients not suitable to a percutaneous approach, ablation can be performed through the video-laparoscopic route, resulting a safe and efficacious method

Resection for HCC The Bologna experience 241 pts. P=0.008 156 pts.

Eligibility for Liver resection BLOG algorithm Cirrhotic patient eligible for liver resection MELD score <9 9 10 >10 Serumsodiumlevel 140 meq/l < 140 meq/l Mortality Major hepatectomy (up to 4 segments) Segmentectomy or bisegmentectomy Segmentectomy or limited resection 0-3.3% 0 0 2.5% Risk of IPLF>15% in all types of hepatectomy Raccomandazioni AISF per la gestione integrata del paziente con Epatocarcinoma; published on www.webaisf.org

AISF PRACTICAL RECOMMENDATIONS Multidisciplinary approach to HCC treatment Dig Liver Dis 2012 (in press) Critical area in clinical decision making for HCC Treatment Surgical Resection (1) The size of the nodule ("single large HCC") has a lesser prognostic impact for resection than for loco-regional therapies [Minagawa, 2007; Shifman,2010; Yang, 2009] Surgical resections is the only available radical treatment for single HCC >5 cm. Always asses its feasibility, preferably in a multidisciplinary setting (3b-B)

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular of HCC based on gene signatures or molecular abnormalities is not ready for clinical application BCLC staging might have some limitation ( Usage of PST in cirrhotic patients, LT in CP stage C, consideration for 2 line & combination treatment) Usage of RFT as first line in stage 0 and extension of indications for surgical resection could be considered on a case by case basis by a multidisciplinary approach

Updated BCLC staging system and treatment strategy HCC Stage 0 PST 0, Child Pugh A Stage A C PST 0 2, Child Pugh A B Stage D PST > 2, Child Pugh C Very early stage (0) 1 HCC < 2 cm Carcinoma in situ Early stage (A) 1 HCC or 3 nodules < 3 cm, PST 0 Intermediate stage (B) Multinodular, PST 0 Advanced stage (C) Portal invasion, N1, M1, PST 1 2 End stage (D) 1 HCC Portal pressure/ bilirubin Increased 3 nodules 3 cm Associated diseases PST 0-1 Normal No Yes Resection Liver transplantation Curative treatments (30%) 5-year survival (40 70%) PEI/RFA TACE Target: 20% OS: 20 mo (45-14) Sorafenib Target: 40% OS: 11 mo (6-14) Best supportive care Target: 10% OS: <3 mo EASL EORTC Clinical Practice Guidelines: Management of hepatocellular carcinoma. Journal of Hepatology 2012 vol. 56 j 908 943

Combined Locoregional Treatments Comparison N. trials: TACE + PEI vs. TACE 4 TACE + RF vs. RF 2 TACE + PEI vs. PEI 1 TACE + RF vs. TACE 1 TACE + PEI vs. TACE o PEI 1 TACE + RF vs. TACE o RF 1 Survival at : - 1 yr: 512 pts. OR = 3.26 (95% CI = 1.23-8.69) - 2 yrs: 437 pts. OR = 4.53 (95% CI = 2.62-7.82) - 3 yrs: 425 pts. OR = 3.50 (95% CI = 1.75-7.02) Recurrence rate Wang W et al., Liver International 2010; 30: 741-749

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular of HCC based on gene signatures or molecular abnormalities is not ready for clinical application BCLC staging might have some limitation ( Usage of PST in cirrhotic patients, LT in CP stage C, consideration for 2 line & combination treatment) Usage of RFT as first line in stage 0 and extension of indications for surgical resection could be considered on a case by case basis by a multidisciplinary approach In non surgical cases a combined approach could be considered

Proposed algorithm for TACE in patients with intermediate-stage HCC Segmental or intratumoral thrombosis? Patient / disease characteristics No PVT No EHS Child-Pugh A or B7 Acceptable? Why? First TACE CT or MRI Liver deterioration or major complications Acceptable? Second TACE Disease control (CR or PR or SD) CT or MRI Disease progression Follow-up / 3 months Consider retreatment with TACE New lesion Growth of existing lesion Consider sorafenib Raoul J-L et al. Cancer Treat Rev 2011;37:212 20

Radioembolization for HCC Patient outcomes according to tumor stage Intermediate stage TACE (Salem, Gastro. 2011) TACE (Wang, Eur J Cancer. 2008) TACE (Chen, Eur J Cancer. 2009) n = 73 n = 741 n = nr TARE (Hilgard, Hepatology. 2010) TARE (Salem, Gastro. 2010) TARE (Sangro, Hepatology. 2011) n = 51 n = 83 n = 87 Sangro B, et al. J Hepatol 2012; 56:464 73

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular of HCC based on gene signatures or molecular abnormalities is not ready for clinical application BCLC staging might have some limitation ( Usage of PST in cirrhotic patients, LT in CP stage C, consideration for 2 line & combination treatment) Usage of RFT as first line in stage 0 and extension of indications for surgical resection could be considered on a case by case basis by a multidisciplinary approach In non surgical cases a combined non surgical aproach could be considered TACE could be considered in patients with peripheral, segmental or intratumoral thrombosis on a case by case basis MRI is the preferred method to assess response to TACE, but equivalent to TC for Drug Eluting Beads-TACE Additional TACE could be considered on a on demand basis ( not repeated in pts with CR) TARE could be considered in patients with tumoral lobar portal vein branches thrombosis

Treatment of Hepatocellular carcinoma: State of the art Key Messages BCLC staging system is recommended for prognostic prediction and treatment allocation and mrecist is the best method to classify treatment response Molecular classification of HCC based on gene signatures or molecular abnormalities is not ready for clinical application BCLC staging might have some limitations ( Usage of PST in cirrhotic patients, LT in CP stage C, consideration for 2 line & combination treatment) Usage of RFT as first line in stage 0 and extension of indications for surgical resection could be considered on a case by case basis by a multidisciplinary approach In non surgical cases a combined approach could be considered TACE could be considered in patients with peripheral, segmental or intratumoral thrombosis on a case by case basis MRI could be the preferred method to assess response to TACE(no DEB TACE) Additional TACE could be considered on a on demand basis ( not repeated in pts with CR) and TARE for pts with tumoral portal branch thrombosis