Ali Ahmady, PhD, MSc, HCLD 1441 Hemingford Lane Mississauga, ON, L5N 7Y7 Tel: Cell:

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1 Ali Ahmady, PhD, MSc, HCLD 1441 Hemingford Lane Mississauga, ON, L5N 7Y7 Tel: Cell: Embryologist with more than 16 years experience in Reproductive biology field seeking a senior position in IVF set up where can utilize scientific knowledge and lab/management experience. SCIENTIFIC ACHIEVEMENTS 1. Produced live-birth from ICSI-derived embryos in the mouse. This is the first report of live-birth following mouse ICSI (Human Reprod., 1995; 10: ). 2. Developed hamster-icsi assay as a test for fertilizing ability of severe male factor sperm prior to clinical ICSI (J. Ass. Reprod., 1996; 13: ). 3. Developed Single Sperm Curling (SSC) test for selection of viable sperm in ICSI procedure (Fertil Steril., 1997; 68: ). 4. Produced live-birth from injection of dead sperm (at the various stage of cell death) in the mouse. This is the first systematic study in fertilising ability of dead sperm (Human Reprod, 1999; 14: ; Human Reprod, 1997; 12: ; J. Exp. Zool., 1999,284: ). EMPLOYMENT AND CAREER PROFILE Astra Fertility Group (formely NUStar Fertility Centre), Mississuga, Ontarion, Canada September 2001 till Present Position: Lab Director Design and equip IVF, Andrology, and chemistry laboratories Establish all procedures including quality control Implement lab policies Oversee day to day operation of the laboratories and provide the direction for the lab staff Managing laboratory budget Negotiation with supplier for purchasing product Recruitment and selection of Laboratory staff Implement procedure manual trouble shooting and problem solving Samuel Lunenfield Research Institute, Mount Sianai Hospital Toronto April 2000-Oct 2001 Position: Post-Doctoral Research Fellow Design and execute research project on stem cell and nuclear reprogramming Establish new procedure Literature search Research seminars 1

2 National University of Singapore (NUS), Singapore Jan 1998-Jan 2000 Position: Research Fellow design and equip a newly research lab recruit lab technician establish protocol coordinate with Zoo to have a joint research on Monkey preparing research proposal for grant application delivering lecturer to postgraduate student (Master programme) organizing the workshop design and perform the research project trouble shooting in all lab procedure reviewing research article for publication Post-graduate examiner National University of Singapore (NUS) Singapore Dec 1992 til Jan 1998 Position: Graduate student design and execute research projects research seminars writing up report and research papers member of the faculty for ART and micromanipulation workshop involve in providing service to the patient Yazd Medical University, Human Reproduction Centre, Iran Jan 1988-Dec 1992 Position: Lab Director and Chief Embryologist Design and equip a newly In Vitro Fertilization (IVF) Lab Producing the first test-tube baby in the country Establishing all lab procedure Overseeing all procedures in the lab Involve in performing the procedure trouble shooting in all lab procedure and problem solving in the lab EDUCATION PhD (Reproductive biology) National University of Singapore, Singapore Thesis titled: The cellular & molecular investigations of fertilising ability of dead sperm. MSc (Reproductive Biology, by research) National University of Singapore, Singapore Thesis titled: Intracytoplasmic Sperm Injection (ICSI) in Mammals. BSc (cellular & Molecular biology) Tehran University, Iran Profession: Cell/Reproductive Biologist, Clinical and Experimental embryologist TECHING EXPERIENCE Lecturer for MCE (Master of Clinical Embryology, one year postgraduate course), Topics include fertilization, embryo development, cell cycle, micromanipulation, nuclear reprogramming (cloning), etc. 2

3 Served as a member of the faculty and Instructor for Course on Assisted Reproductive Techniques ( ) conducted by Department of Obstetrics & Gynaecology, National University of Singapore. Topics related general ART and specialised techniques (micromanipulation, co-culture, zona-free hamster test). Served as a member of the faculty, Lecturer and Instructor for Advanced course on Micromanipulation ( ) conducted by Department of Obstetrics & Gynaecology, National University of Singapore. topics related to micromanipulation (preparation of micro- injection and holding pipette; technique of ICSI, SUZI, PZD; blastomere removal, enucleation, cloning, PGD, etc.). External Examiner for PhD thesis, Manipal University, Karnataka, India, 1999 AWARD/ CERTIFICATE 1. HCLD (High Complexity Lab Director), ABB, USA Second prize of the first Royan International Infertility Research Award, Tehran, Iran, Sep Authorised by Ministry of Health, Singapore, to perform laboratory procedures in IVF and other Assisted reproduction Technologies (ART) in Singapore January 1996-Dce National University of Singapore to pursue postgraduate studies (covered tuition fees and stipend), PROFICIENCY IN LABORATORY TECHNIQUES 1. Mouse/hamster preimplantation embryology (superovulation/generation, recovery, culture and embryo transfer). 2. Human oocyte handling, identification, fertilization, culture of resultant embryo, and embryo transfer. 3. Sperm suspension preparation/recovery from human semen and from mouse epididymis for in vitro fertilization, IUI, GIFT, etc. 4. Cryopreservation of mouse and human sperm, embryos and hamster oocytes. 5. Proficient in microtool fabrication (microinjection and holding needles) for micromanipulation purpose. 6. Proficient in intracytoplasmic sperm injection (ICSI) in mouse, hamster, human. 7. Electrofusion/-poration techniques for assisted reproduction. 8. Zona-free hamster eggs penetration test. 9. Basic molecular biology techniques. 10. Spermatid and testicular sperm preparation from human /mouse testes biopsies for intracytoplasmic injection into oocytes. 11. FITC (Determination of acrosome reaction in sperm cells) 12. Cytogenetic preparation of oocytes and embryos. 13. Preparation of sperm, oocytes and embryos for electron microscopy studies. 14. Evaluation of ICM and trophectoderm cell number in the mouse blastocyst. 15. Detection of DNA strand break using TUNEL (end-labeling tailing) test. 3

4 16. Enucleation, and nuclear reprogramming procedures. 17. Setting up and culturing embryonic stem cell. 18. Vitrification of oocytes and embryos 19. In vitro maturation Computer Knowledge MS office (Word, PowerPoint, Excel, Access) Internet explorer, Netscape, Outlook, Photoshop, Pagemaker, Adobe Acrobate, computer maintenance and house keeping PUBLICATIONS: 1 Karimzadeh, M and Ahmadi, A. The fundamental of in vitro Fertilization (book published, 1992, in Persian Language), Iran, Ahmadi, A; Ng, SC; Bongso, A; Liow, SL and Ratnam, SS. Induction of acrosome reaction in human sperm by an electrofusion current. Singapore J. Obst. Gyna., Singapore, 1994; 25: Ahmadi, A; Ng, SC; Liow, SL; Ali,J; Bongso, A and Ratnam, SS. Intracytoplasmic sperm injection (ICSI) of mouse oocytes with 5 mm Ca ++ at different intervals. Human Reprod., UK, 1995; 10 : Ng, SC; Liow, SL, Ahmadi, A, Yong, EL, Bongso, A and Ratnam, SS. Intracytoplasmic sperm injection-is there a need for an animal model, specially in assessing the genetic risks involved. Human Reprod., 1995; 10: Ng, SC; Liow, SL, Ahmadi, A, Bongso, A; Kumar, J; Chan, CLK and Ratnam, SS. The use of non-contact microbeam laser for assisted reproduction. IX world congress on IVF and ART. 3-7 April 1955: Ng, SC; Liow, SL; Ahmadi, A; Bongso, A; Chan, CLK; Kumar, J; Yong, EL and Ratnam, SS. Intracytoplasmic sperm injection (ICSI) in severe male factor patients and patients with failed fertilization without male factor: Experience from the National Univ3ersity Hospital, Singapore. J. Asst. Reprod. Genet. Suppl., 1995; 12 (3): 83S. 7. Ng, SC; Liow, SL; Ahmadi, A; Bongso, A. New perspectives in understanding fertilization in the era of microinjection. J. Mamm. Ova Res., Iapan, 1996; 13: S Ahmadi, A; Bongso, A and Ng, SC. Intracytoplasmic injection of human sperm into the hamster oocyte (hamster-icsi assay): as a test for fertilizing capacity of the severe male factor sperm. J. Ass. Reprod., 1996; 13:

5 9 Ahamdi, A; Bongso, A and Ng, SC. Exposure of human sperm to electrical field: effects on acrosome reaction. Penetration rate and motility. ARTA, USA, 1996; 9: Ahmadi, A; Bongso, A and Ng, SC Intracytoplasmic injection of human sperm into the frozen-thawed hamster oocytes. Med. Sc. Res., UK, 1996; 24: Ng, SC; Liow, SL; Ahmadi, A, Ho J. Sperm microinjection. In: Visual atlas of human sperm structure and function for assisted reproductive technology, Sathananthan AH (ed), NUS, Singapore, 1996; pp Ahmadi, A; Bongso, A and Ng, SC. Induction of Acrosome reaction in human sperm by exposing to an electrical field. Arch. Androl., USA, 1997; 38: Ahmadi, A and Ng, SC. Single Sperm Curling (SSC) test, a modified hypoosmotic swelling test, as a potential technique for selection of viable sperm in ICSI procedure. Fertil Steril., USA, 1997; 68: Ahmadi, A and Ng, SC. Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondrial-damaged sperm in mammals. Zygote,UK, 1997; 5: Ahmadi, A and Ng, SC. Fertilization and development of mouse injected with membrane-damaged sperm. Human Reproduction; UK, 1997; 12: Ahmadi, A and Ng, SC. Influence of sperm plasma membrane destruction on human sperm head decondensation and pronuclear formation.arch Androl., USA, 1999; 42: Ahmadi A, and Ng SC. Developmental capacity of damaged spermatozoa. Human Reprod, 1999; 14: Ahmadi, A and Ng, SC. Destruction of protamine in human sperm inhibit sperm binding and penetration in zona-free haster penetration test, but increase sperm head decondensation and male pronuclear formation in hamster-icsi assay. J. Assist. Reprod. Genet., 1999; 16: Ahmadi, A and Ng, SC. Fertilizing ability of apoptotic (DNA-damaged) spermatozoa. J. Exp. Zool., 1999,284: Ahmadi, A and Ng, SC. Is biologically dead sperm reproductively dead. Biol. Reprod., 1999; 60 (Suppl. 1): 105 (abst. 36).. References Available upon request 5