Curriculum Vitae: Raul J. Cano, Ph.D.
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1 CurriculumVitae:RaulJ.Cano,Ph.D. I.PERSONALINFORMATION NAME: RaulJ.Cano OFFICEADDRESS: BiologicalSciencesDepartment,53 210E CaliforniaPolytechnicStateUniversity SanLuisObispo,CA93407 OFFICETELEPHONE: (805) FAX: (805) Cell (805) E MAIL: [email protected] II.FORMALEDUCATION PRECOLLEGIATE: MaristasBrothers LaHabana,Havana,Cuba Yearsattended: HIGHEREDUCATION UNIVERSITY UniversityofMontana Missoula,Montana YEARS ATTENDED UniversityofSeville EasternWashingtonUniversity EasternWashingtonUniversity SpokaneFallsCommunityCollege III.PROFESSIONALEXPERIENCE OCCUPATIONANDPOSITIONSHELD 1996 present 2000 present present 1987 present 1983 present CanoVitae DEGREEEARNED Ph.D. Microbiology M.S. Clinical/MolecularMicrobiology M.S. Biology FungalGenetics B.S. BiologicalSciences A.A. LiberalArts Director,EnvironmentalBiotechnologyInstitute,CaliforniaPolytechnic StateUniversity,SanLuisObispo,California. UnocalChairforEnvironmentalStudies.EndowedChair,California PolytechnicStateUniversity,SanLuisObispo,California. FounderandPresident,EnvironmentalDiagnostics,Inc., SanLuisObispo,CA Founder,ChiefScientificOfficer,AmbergeneCorporation SanCarlos,CA VisitingResearcher.DepartmentofMicrobiology,SchoolofMedicine, UniversityofSeville,Seville,Spain. VisitingResearcher,DepartmentofMicrobiology,UniversityoftheBalearic Islands,PalmadeMallorca,Spain. ProfessorofMicrobiology.CaliforniaPolytechnic StateUniversity,SanLuisObispo,CA
2 CURRENTRESEARCH Microbialdiversityandsignatureanalysisofmarinesedimentsandothersubsurfacesamplesfor naturalproductdiscoveryandpetroleumexplorationefforts. Biologicalreclamationofwasteglycerolfrombiodieselproduction CONSULTING UnocalCorporation,FullertonCA UnocalCorporation,SugarLand,TX ChevronCorporation,SanLuisObispo,CA Lanahan&Reilley,LLP,SanJose,CA Gavin&Cunningham,PaloAlto,CA LewisBrisboisBisgaardSmith,SanJose,CA Robinson&Wood,LLP MagellanBiosciences,Tampa,FL SheaHomes,SanJose,CA LaPlante&Spinelli,Donald,Nott,Sacramento,CA NereusPharmaceuticals,Inc.,SanDiego,CA XOMA(US)LLC,Berkeley,CA RocheDiagnostics,Inc.,Spain,Switzerland,Alameda,CA MicrocidePharmaceuticals,Inc.,MountainView,CA Intrabiotics,Inc.,MountainView,CA AppliedBiosystems,Inc.,FosterCity,CA DakoCorporation,Carpinteria,CA Nunc,Copenhagen,DK CERTIFICATESANDLICENSES PublicHealthMicrobiologist.Certifiedin1978byCaliforniaDepartmentofPublicHealthServices FOREIGNLANGUAGES Spanish SCIENTIFICAFFILIATIONS AmericanSocietyforMicrobiology AmericanAcademyforMicrobiology InternationalSocietyforHumanand AnimalMycology SocietyforIndustrialMicrobiology InternationalSocietyofEnvironmental Forensics AmericanConferenceofGovernmental IndustrialHygienists EuropeanSocietyforMicrobiology IV.GRANTS,AWARDS,PATENTS GRANTSANDCONTRACTS 2003MicrobialDiversityofPetroleumContaminatedSoilsandGroundwater,UNOCALCorporation, $431,000. CanoVitae
3 CanoVitae MicrobialDiversityofMarineActinobacteria,NereusPharmaceuticals,Inc.$33, ElucidationoftheroleofAnctinobacteriaonthebioremediationofpetroleumcontaminated sands.unocalcorporation,$219, DevelopmentofaMolecularAnalysistoolforthecharacterizationofactinobacterialcommunities inenvironmentalsamples.unocalcorporation,$209, ConstructionofEBIResearchFacility,UNOCALCorporation,$2,000, LactobacillusAcidophilusGenomeSequencingandFunctionalAnalysis.RhodiaCorporation, $211, PhytoremediationofC8plume.(3 yearstudy)unocalcorporation$1,500, LactobacillusAcidophilusGenomeSequencingandFunctionalAnalysis.RhodiaCorporation, $211, AssesmentofPetroleum degradingdiversityinsanddunes.unocalcorp.$280, BioinformaticsofLactobacillusacidophilus.SUNMicrosystems,Inc.$29, Lactobacillusacidophilusgenomeproject.DairyMangement,Inc.,$407, EnvironmentaldetectionofListeriaspp.byTaqManPCR.,DairyMangement,Inc.,$146, MicrobialDiversityStudiesinsanddunes.UNOCAL,$1,316, additionalgrants($288,950.00) AWARDS 2003CaliforniaPolytechnicStateUniversityPresident sdiversityaward 2001MDS/PharmaBiotechnologyResearchAward 2001Burroughs WellcomeFundVisitingProfessorship 2000UnocalChairforEnvironmentalStudies 1997CarskiFoundationDistinguishedTeacherAward. 1998Fellow.AmericanAcademyofMicrobiology 1998FoundationforMicrobiologyDistinguishedLecturer 1997MeritoriousPerformanceAward. 1996MeritoriousPerformanceAward. 1994CaliforniaPolytechnicStateUniversityOutstandingProfessorAward. 1994TheCaliforniaStateUniversityTrusteesOutstandingProfessorAward. 1993TheCaliforniaStateUniversityBiotechnologyResearchLecturerAward 1992SummerResearchFellowship,TheCaliforniaStateUniversity 1991SummerResearchFellowship,TheCaliforniaStateUniversity 1990MeritoriousPerformanceAwardTheCaliforniaStateUniversity. 1989MeritoriousPerformanceAward.TheCaliforniaStateUniversity. 1987MeritoriousPerformanceAward.TheCaliforniaStateUniversity. PATENTS Ancientbacteriafromamberinclusions DNAArraychipforthedetectionofpetroleumdegradersinecosystems. (Pending) InductionofClassIandClassIIIHeatShockGenestoProvideStressProtectiontoLactobacillus acidophilus. V.PUBLICATIONS PEERREVIEWEDJOURNALS OMcAuliffe,RJCano,TRKlaenhammer.2005.GeneticAnalysisofTwoBileSaltHydrolaseActivitiesin LactobacillusAcidophilusNCFM..ApplEnvironMicrobiol71(8):4925 9
4 CanoVitae MAAzcarate Peril,EAltermann,RLHoover Fitzula,RJCano,TRKlaenhammer.2004.Identificationand InactivationofGeneticLociInvolvedwithLactobacillusacidophilusAcidTolerance.ApplEnviron Microbiol70(9): EAltermann,LBBuck,RCano,TRKlaenhammer.2004.IdentificationandInactivationofGeneticLoci InvolvedwithLactobacillusAcidophilusAcidTolerance.Gene342(1): Title:PSYeung,CLKitts,RCano,PSTong,MESanders.2004.ApplicationofGenotypicandPhenotypic AnalysestoCommercialProbioticStrainIdentityandRelatedness.JApplMicrobiol97(5): MDBraid,JLSilhavy,CLKitts,RJCano,MMHowe.2004.CompleteGenomicSequenceofBacteriophage B3,aMu likephageofpseudomonasaeruginosa:jbacteriol.186(19): CLGreenblatt,JBaum,BYKlein,SNachshon,VKoltunov,RJCano.2004.MicrococcusLuteus Survival inamber.microbecol48(1):120 7 EAltermann,WMRussell,MAAzcarate Peril,RBarrangou,BLBuck,OMcAuliffe,NSouther,ADobson,T Duong,MCallanan,SLick,AHamrick,RCano,TRKlaenhammer.2004.CompleteGenomeSequence oftheprobioticlacticacidbacteriumlactobacillusacidophilusncfm.procnatlacadsci102(11): RBarrangou,EAltermann,RHutkins,RCano,TRKlaenhammer.2003.FunctionalandComparative GenomicAnalysesofanOperonInvolvedinFructooligosaccharideUtilizationbyLactobacillus acidophilus.procnatlacadsciusa100(15): TKlaenhammer,EAltermann,FArigoni,ABolotin,FBreidt,JBroadbent,RCano,etal.2002.Discovering LacticAcidBacteriabyGenomics.AntonieVanLeeuwenhoek82:29 58 Quereshi,A.,Mugher,J.,Cano,R.,Galazzo,J,andLee,M.2001.MF EA 705aandMF EA 705b,New fungalmetabolitesfrommicrobialfermentationofastreptomycessp.j.antibiotics.54: Cano,R.J.F.Rollo,M.Ubaldi,C.delCueto,S.Luciani,T.Cox,P.Orkand,K.H.KŸnzel,andF. Tiefenbrunner.2000.SequenceanalysisofbacterialDNAinthecolonandstomachoftheTyrolean Iceman.AmJPhysAnthropol.112(3): GreenblattC.L,A.Davis,B.G.Clement,C.L.Kitts,T.Cox,andR.J.Cano.1999.MicrobialDiversityof Amber.MicrobialEcology38: Cox,T;Frazier,C;Tuttle,J;Flood,S;Yagi,L;Yamashiro,CT;Behari,R;Paszko,C;Cano,RJ.1998.Rapid detectionoflisteriamonocytogenesindairysamplesutilizingapcr basedfluorogenic5'nuclease assay.j.industrialmicrobiol.biotechnol.21(3): UbaldiM,LucianiS,MarotaI,FornaciariG,CanoRJ,RolloF.1998.SequenceanalysisofbacterialDNAin thecolonofanandeanmummy.amjphysanthropol107(3): Cox,T.,Frazier,C.,Flood,S.andR.J.Cano.1998.DevelopmentofaFluorescent5 ExonucleaseAssay forthedetectionoflisteriamonocytogenesinfoodanddairyproducts.j.industrialmicrobiol. Biotechnol. Lambert,L.H.,T.Cox,K.Mitchell,R.Rossello Mora,C.delCueto,D.E.Dodge,P.Orkand,andR.J.Cano Staphylococcussuccinussp.nov.fromDominicanAmber.Int.J.Sys.Bacteriol.48: Cano,R.J.1997.Isolation,characterization,anddiversityofmicroorganismsfromamber.SPIE Symposium,SanDiego,CACano,R.J.1996RecoveryandCharacterizationofAncientBacillus sphaericusfrom DominicanAmber.1996.Quimica.60:8 14.Cano,R.J.1996.CharacterizingAncientmicroorganisms fromamber.analyticalchemistry68:609a 611.Cano,R.J.1996.AnalysingAncientDNA Endeavour.20: Cano,R.J.andM.Borucki.1995.Ageofbacteriainamber.Science270: Cano,R.J.andM. Borucki.1995.IsolationofviableBacillussp.fromDominicanamber(25 40million years old). Science268: Cano,R.J.1994.AncientBacillusDNA:Awindowtoancientsymbioticrelationships?ASMNews. 60(3):
5 CanoVitae Cano,R.J.,Norton,D.,Inzunza,A,andOste,C.1995.FluorescentdetectionPCRofListeria monocytogenesindairyfoods.journaloffoodprotection58: Cano,R.J.,Borucki,M.,Schweitzer,M.,Poinar,H.,Poinar,G.O.,andPollard,K.1994.AncientBacillus DNA:Awindowtoancientsymbioticrelationships?AppliedandEnvironmentalMicrobiolgy. 60(6): Palomares,J.C.andR.J.Cano Antisense therapyandclinicaldiagnostics:areview. EnfermedadesInfecciosasyMicrobiolog acl nica11(9): Rasmussen,S.,Rasmussen,H.,Larsen,M.,Hoff J rgensen,r.,andcano,r.j.1994.combined PolymeraseChainReaction HybridizationMicroplateAssayfortheDetectionofBovineLeukemia VirusandSalmonella.AmplificationandHybridizationtakeplaceinthesamemicrowell.Clinical Chemistry40(2): Alados Arboleda,J.C.,Miranda,C.,Ortiz,F.andCano,R.J.1994.DisseminatedHistoplasmosisinSpain. Eur.J.Clin.Microbiol.Infect.Disease.12: Cano,R.J.,H.N.Poinar,N.SPieniezak,andG.O.Poinar,Jr.1993.Enzymaticamplificationandnucleotide sequencingofdnafrom milionyearoldweevilNature363: Poinar,H.N.,PoinarJr.,G.O.,andCano,R.J.1993.Molecularphylogenyofanextinctlegume (Hymenaeaprotera)fromDominicanamber.Nature363:677. Cano,R.J.andH.N.Poinar.1993.RapidIsolationofDNAfromFossilandMuseumSpecimensSuitable forthepolymerasechainreaction.biotechniques.15: Cano,R.J.1993.FluorescentdetectionofSalmonella.LabMedicaInternational.6:25 33 Cano,RJ,RasmussenSR,SanchezG,Palomares,JC1993:FluorescentDetection polymerase chainreaction(fd PCR)AssayonMicrowellPlatesasascreeningtestforSalmonellasinfoods.J.Appl Bacteriol75: Poinar,G.O.,H.N.Poinar,andR.J.Cano.1993.ExtractionofDNAfromAmberInclusions.AncientDNA Newsletter2: Cano,R.J.,H.N.Poinar,D.W.Roubick,andG.O.Poinar,Jr.1992.Enzymaticamplificationandnucleotide sequencingofportionsofthe18srrnageneofthebeeproplebeiadominicana (Apidae:Hymenoptera)isolatedfrom25 40milionyearoldDominicanamber.MedicalScience Research.20(17): Torres,M.J.,Cano,R.J.,Rodr guez,a.,andpalomares,j.c.1992.detectionofchlamydiatrachomatisby DNA RNAhybridization.EnfermedadesInfecciosasyMicrobiolog acl nica10(3): Cano,R.J.,Palomares,J.C.andTorres.M.J.1992.EvaluationofafluorescentDNAhybridizationassayfor thedetectionofneisseriagonorrhoeae.europeanjournalofclinicalmicrobiologyandinfectious Diseases11: Cano,R.J,Torres,M.J.,andPalomares,J.C.1992.DetectionofSalmonellasppbyEI PCR.Enfermedades InfecciosasYMicrobiolog acl nica10(3)42 44 Cano,R.J.,Poinar,H.,andPoinar,Jr.G.O.1992.IsolationandPartialCharacterizationofDNAfromthe beeproplebeiadominicana(apidae:hymenoptera)in25 40 millionyearoldamber.medicalscience Research20: additionalresearch(peer reviewed)publications OTHERPUBLICATIONS Cano,R.J.2002.TheMicrobiologyofSpore formingbacteriainamber.in:archaeologyofinfectious diseases.greenblatt,c.editor.jerusalem,israel. Cano,R.J.andKlaenhammer.T.2002.TheCompleteGenomeofLactobacillusacidophilus.7 th
6 InternationalConferenceofLacticAcidBacteria.Amsterdam. Cano,R.J.2000.BiomolecularMethods.In:MolecularMethodsinAncientDNAstudiesofArt.E. CilibertoandG.Spotto,eds.WileyInterscience,NewYork,NY.BalabanPublishers,Jerusalem,Israel Stansfield,W.,R.J.Cano,andJ.S.Colome.1996.Schaum soutlinesinmolecularbiology.johnwiley, NewYork. Cano,R.J.1996.M.EnzymaticamplificationofancientDNA.Nucleicacidamplificationmethodsforthe anlysesofenvironmentalsamples.technomicpublishingco,inc. Poinar,G.O.,H.N.Poinar,andR.J.Cano.1993.DNAfromAmberInclusions.HerrmannandHummeleds. pp SpringerVerlag,Berlin. Cano,R.J.1988.LaboratoryExercisesinMolecularBiology.SecondEdition.CaliforniaPolytechnicState University. Cano,R.J.andJ.S.Colome.1988.EssentialsofMicrobiology.WestEducationalPublishing,St.Paul,MN. Cano,R.J.andJ.S.Colome.1986.Microbiology.WestEducationalPublishingCompany.St.Paul,MN. Colome,J.,A.M.Kubinski,R.J.Cano,andD.Grady.1986.LaboratoryExercisesinMicrobiology.West EducationalPublishingCompany.St.Paul,M. 18additionalpublications STANDINGCOMMITTEES GuidelinesCommittee.AmericanAcademyforMicrobiology. Chairman,ResearchandProfessionalDevelopment,CaliforniaPolytechnicStateUniversity. ScientificAdvisoryBoardNationalCenterforToxicologicalResearch,FoodandDrugAdministration. ScientificAdvisoryBoardXOMA(US)LLC,Berkeley,CA. ExecutiveCommittee,CSUProgramforResearchandEducationinBiotechnology.CaliforniaState University(CSU),Sacramento,CA. Chairman,Board,EnvironmentalDiagnostics,Inc.SanLuisObispo,CA. BoardMember,CHOSeNCorporation,Fremont,CA. Chairman,BiotechnologyIndustrialAdvisoryBoard,CollegeofSciencesandMathematics,CalPoly,San LuisObispo,CA. CanoVitae
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