LABORATORY 2 Microbial Diversity; Effectiveness of Hand Washing; Pure Culture Subculture; Light Microscopy
|
|
- Alban Long
- 7 years ago
- Views:
Transcription
1 LABORATORY 2 Microbial Diversity; Effectiveness of Hand Washing; Pure Culture Subculture; Light Microscopy Today s lab looks at the results of microbial diversity and continues the project of working toward producing a pure culture. The effectiveness of hand washing is also examined. New activities include microscopy with viewing and measuring the sizes of bacterial cells. The objectives of today's laboratory period are to: 1. Discover the diversity of bacteria and fungi in the environment and on our bodies. 2. Use the 4-way streak plate from a mixed culture to pure culture one of the bacterial isolates. 3. Evaluate the effectiveness of hand washing. 4. Master the use of the light microscope. Activity 1: Discovering the Diversity of Invisible Microbes Obtain your individual agar plate and group broth tubes from the back of the lab. 1. Examine the plate (DO NOT OPEN THE LID!) and observe the bacterial and/or fungal colonies growing. Streak Plate of Environmental/Body Specimen Draw a representative sample of the colonies on your plate. How can you tell a bacterial colony from a fungal colony? 10
2 2. Examine your group s broth tubes. Is the control tube still sterile? How do you know it is/is not sterile? Contrast the usefulness of a T-soy agar plate culture versus a T-soy broth culture. Project 1: Making a Pure Culture (Part II) By consulting with Dr. P, use your streak-plate to subculture one of the three bacterial species [Serratia marcescens, Micrococcus luteus, or Escherichia coli] onto a sterile T-soy agar slant using aseptic technique. Subculture: Refers to the transfer of microbes from one culture medium to another. Materials Needed Individual streak plate 1 T-soy agar slant (per student) 4-Way Streak Plate Draw a representative sample of the colonies present on your streak plate. The bacterial species I am going to try to pure culture is: Procedure To subculture the identified bacterial species: 1. Sterilize your transfer loop in the burner flame until the loop turns orange and then let the loop cool for about seconds. 2. Using your streak plate, gently scrape up the identified colony with your sterile transfer loop. 3. Immediately streak the loop over the agar surface of the T-soy agar slant. 4. Label the tube and place your slant in the test tube rack on the 22 C incubation tray. 11
3 Activity 2: The Effectiveness of Hand Washing Obtain your group s hand washing T-soy agar plates and determine how effective hand washing was at reducing the number of bacteria on one s hands. Be ready to give a short one minute report to the class. Activity 3: Examination of Stained Bacterial Cell Preparations with the Light Microscope A. Essential Features of the Light Microscope. You will be using a light microscope in some of the labs this semester. This instrument contains several essential features. Label the essential features of the microscope on the drawing below. Par focal: Working distance: Artifact: 12
4 Calculating Total Magnification Objective Lens Ocular Lens Total Magnification 10X (low power) 40X (high power) 100X (oil immersion) 10X 10X 10X B. Focusing with the Microscope. To observe specimens in the light microscope, follow the steps listed below. 1. Remove the microscope from the cabinet. Always pick up your microscope correctly; grasp it firmly with two hands, one hand under the base and the other on the arm. 2. Place the microscope on your lab bench, away from the edge. Move it to a position in which you can look comfortably through the oculars (eyepieces). 3. Make sure the low-power (10X) objective is in place over the hole in the stage. Use the coarse adjustment knob to lower the stage so it is as low as it will go. 4. Turn on the microscope light ("I" on switch) and turn the dial to 6 (brightest). Continued on next page 13
5 5. Place your specimen slide on the stage and carefully secure it in the mechanical stage. Using the mechanical stage knobs, adjust the position of the sample so that it is over the center of the condenser lens. Make sure the low-power (10X) objective is in position. 6. With the course adjustment knob, raise the stage all the way up. Look through the oculars and use the coarse adjustment knob to focus on the specimen. You will not have to turn the knob very far. When the specimen is in view, use the fine adjustment knob to bring it clear, sharp focus. If necessary, move the specimen into the center of the field. 7. To use the oil-immersion (100X) objective, move the 100X lens into the viewing position. Add a drop of immersion oil to the slide under the objective lens. You should focus on the specimen using only the fine adjustment knob. 8. While at 1,000X total magnification, make all drawings, size measurements, and any other notes in your lab manual. Measuring size: at 1,000X, each division (tic mark) on the scale bar = 1µm (micrometer) If you are going to look at another specimen, leave the microscope light on, return to the low-power objective, and lower the stage before removing the slide. Repeat steps 5 through When you are finished using the microscope for the lab period: Make sure the low-power (10X) objective is in position. Lower the stage completely. Turn off the microscope light (O on switch) and turn the dial to 1. Put the microscope in the proper cabinet location. C. Observation of Prepared Slides. Using the focusing steps outlined above, observe and draw representative examples of each of the following bacterial species in the boxes below. Materials Needed Prepared slides of: Micrococcus luteus: A normal bacterial member of our skin microbiome. Neisseria gonorrhoeae: The causative bacterial agent of gonorrhea, a sexually transmitted disease in humans. Rhodospirillum rubrum: A photosynthetic bacterial species found in aquatic environments (ponds, mud, and sewage). Candida albicans: A fungus that grows both as yeast (oval-shaped) cells and as filaments; causative agent of candidiasis ( yeast infections ) in humans. 14
6 Procedure To observe each prepared slide: 1. Make sure the prepared slide is clean and dry before placing it on the microscope stage. 2. Examine the slide following the focusing steps outlined above. Under 1,000X total magnification (oil immersion), record (in the table provided): a. Morphology (rod, sphere, or wavy spiral) b. Relative size (in micrometers; µm) Morphology: Refers to cell shape. Observations of Prepared Slides Micrococcus luteus Morphology: Neisseria gonorrhoeae Morphology: Rhodospirillum rubrum Morphology: Candida albicans Morphology of oval cells: 15
7 LIGHT MICROSCOPE REVIEW QUESTIONS 1. What is the purpose for each of the following parts of the light microscope? a. Course and fine focusing knobs b. Iris diaphragm (contained in condenser lens) c. Mechanical stage d. Objective lenses e. Oculars (eyepieces) 2. A classmate missed the lab explaining the use of the microscope and, as such, is having some difficulties using the instrument. Identify how you would attempt to help the student correct the following problems. a. Inability to bring the specimen into sharp focus. b. Insufficient light while viewing the specimen under 1,000X (oil immersion). c. Artifacts in the microscope field. 16
Microscopy and Cellular Morphology
Microscopy and Cellular Morphology As we discussed in class, many organisms on the planet exist as single cells and are referred to as microorganisms bacteria, protozoans, among others. When a single microorganism
More informationExercise 2. The Compound Light Microscope
6 Exercise 2 The Compound Light Microscope INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Demonstrate proficient use of the microscope using low, high dry,
More informationEXPERIMENT #1: MICROSCOPY
EXPERIMENT #1: MICROSCOPY Brightfield Compound Light Microscope The light microscope is an important tool in the study of microorganisms. The compound light microscope uses visible light to directly illuminate
More informationTransferring a Broth Culture to Fresh Broth
Sterile Technique It is very important in microbiology to work with pure cultures. Unfortunately this is difficult. The world around us is covered with microorganisms. Microorganisms are even carried on
More informationMICROSCOPY. To demonstrate skill in the proper utilization of a light microscope.
MICROSCOPY I. OBJECTIVES To demonstrate skill in the proper utilization of a light microscope. To demonstrate skill in the use of ocular and stage micrometers for measurements of cell size. To recognize
More informationName Class Date Laboratory Investigation 4B Chapter 4: Cell Structure
Name Class Date Laboratory Investigation 4B Chapter 4: Cell Structure The Microscope: A Tool of the Scientist You may refer to pages 66-67, 72-73 in your textbook for a general discussion of microscopes.
More informationCare and Use of the Compound Microscope
Revised Fall 2011 Care and Use of the Compound Microscope Objectives After completing this lab students should be able to 1. properly clean and carry a compound and dissecting microscope. 2. focus a specimen
More informationMicrobiology Laboratory Safety and Basic Procedures Safety in a microbiology laboratory is important in the prevention of infection that might be
Microbiology Laboratory Safety and Basic Procedures Safety in a microbiology laboratory is important in the prevention of infection that might be caused by the microorganisms being studied. This laboratory
More informationLab Exercise 3: Media, incubation, and aseptic technique
Lab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. 2. Describe the different formats of media, plate, tube etc. 3. Explain how to sterilize it,
More informationIn order to be useful, a smear must have the following qualities:
Smear Preparation and Simple Stain Objectives: Make bacterial smear slides (usually called smears) Distinguish cells on these slides using a simple stain procedure Unstained microbial cells are nearly
More informationWelcome to Implementing Inquirybased Microbial Project. Veronica Ardi, PhD
Welcome to Implementing Inquirybased Microbial Project Veronica Ardi, PhD Microbiology Laboratory Courses CourseSmart: ebook resources http://instructors.coursesmart.com/ Microbiology Laboratory Courses
More informationMICROSCOPY OF LIVING MICROBES
EXPERIMENT 1 MICROSCOPY OF LIVING MICROBES Many students taking microbiology for the first time feel that they are going to have a hard time with the microscope. This lab as an experiment is intended to
More informationTHE COMPOUND MICROSCOPE
THE COMPOUND MICROSCOPE In microbiology, the microscope plays an important role in allowing us to see tiny objects that are normally invisible to the naked eye. It is essential for students to learn how
More informationMITOSIS IN ONION ROOT TIP CELLS: AN INTRODUCTION TO LIGHT MICROSCOPY
MITOSIS IN ONION ROOT TIP CELLS: AN INTRODUCTION TO LIGHT MICROSCOPY Adapted from Foundations of Biology I; Lab 6 Introduction to Microscopy Dr. John Robertson, Westminster College Biology Department,
More informationBIO 10 Lab 1 Introduction Pre Lab Test
BIO 10 Lab 1 Introduction Pre Lab Test 1. Why is the microscope in our lab called a compound microscope? 2. How do you calculate total magnification? 3. What is the lowest and the maximal magnification
More informationBACTERIAL ENUMERATION
BACTERIAL ENUMERATION In the study of microbiology, there are numerous occasions when it is necessary to either estimate or determine the number of bacterial cells in a broth culture or liquid medium.
More informationBiological Sciences Initiative
Biological Sciences Initiative HHMI Student Activities Measuring Antibiotic Resistance Introduction: You might be aware that antibiotics were once thought of as a magic bullet; a nearly perfect drug for
More informationCompound microscope (Hund)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 Compound microscope (Hund) 15 16 17 18 19 20 1) Eyepieces (magnifies 10x), one with diopter adjustment, 2) Interp[upillary adjustment, 3) Head, 4) Revolving nosepiece,
More informationAdapted from Biology 15 Laboratory Supplemental Manual: Wrightsman, Ininns and Cannon- Moloznic.
Biology 3B Laboratory Cultural Characteristics of Bacteria Objectives: Describe bacterial structure: colony morphology, cell shape, growth patterns. To distinguish how various growth media will affect
More informationMicroscope Lab Introduction to the Microscope Lab Activity
Microscope Lab Introduction to the Microscope Lab Activity Wendy Kim 3B 24 Sep 2010 http://www.mainsgate.com/spacebio/modules/gs_resource/ CellDivisionMetaphase.jpeg 1 Introduction Microscope is a tool
More informationDETECTION OF BACTERIAL MOTILITY. To demonstrate bacterial motility by microscopic and macroscopic techniques.
DETECTION OF BACTERIAL MOTILITY I. OBJECTIVES To demonstrate bacterial motility by microscopic and macroscopic techniques. To observe flagella in prepared slides stained by specific flagellar stains. II.
More informationMT-30 & MT-90 Series. Advanced Academic Microscopes/ Advanced Academic Polarizing Microscope INSTRUCTION MANUAL
Introduction With your purchase of an MT-30/MT-90 series type microscope you have chosen for a quality product. The MT-30/MT-90 series type microscopes are developed for use at schools and laboratories.
More informationHOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY
HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results
More informationBiology 342 - Microbiology Lab Manual
Biology 342 - Microbiology Lab Manual Enteric bacteria cultured on Triple Sugar Iron agar slants. Gas production has lifted the agar in the tube on the left. Bacteria in the tube on the right are producing
More informationPURE CULTURE TECHNIQUES. To demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials.
PURE CULTURE TECHNIQUES I. OBJECTIVES To demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. To demonstrate skill in isolation of organisms from a mixed
More informationGreen Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein
Green Fluorescent Protein (GFP): Genetic Transformation, Synthesis and Purification of the Recombinant Protein INTRODUCTION Green Fluorescent Protein (GFP) is a novel protein produced by the bioluminescent
More informationCell Biology Prokaryotic and eukaryotic cells
Cell Biology Prokaryotic and eukaryotic cells Observation of cells and organelles In this lab you will be looking at an example of a Prokaryotic cell (Bacillus cereus) and a some examples of Eukaryotic
More informationChapter 1 Parts C. Robert Bagnell, Jr., Ph.D., 2012
Chapter 1 Parts C. Robert Bagnell, Jr., Ph.D., 2012 Figure 1.1 illustrates the parts of an upright compound microscope and indicates the terminology that I use in these notes. Figure 1.1. Parts of a Compound
More informationPre-Lab Questions. 1. What is cell theory? 2. What do all cells contain? 3. What is a prokaryote? 4. What is a eukaryote? 5. What is an organelle?
Name: TOC# Background Ever since the first microscope was used, biologists have been interested in studying the cellular organization of all living things. After hundred s of years of observations by many
More informationLab Exercise 2 Media and Culture
Lab Exercise 2 Media and Culture Lab Exercise #2 Bacterial Media & Culture I. OBJECTIVES: Practice microbial collection techniques Describe colony morphology and the relationship to microbial identification.
More informationMicroscopes and the Metric System
Microscopes and the Metric System BIO162 Fall 2007 Sizes of Microorganisms: -Viruses: 0.01 0.3 um -Bacteria: 1 3 um -Fungi: 3 30 um -Protozoa: 5 1000 um 1 Measuring Microorganisms Ocular Micrometer The
More informationTransformation of the bacterium E. coli. using a gene for Green Fluorescent Protein
Transformation of the bacterium E. coli using a gene for Green Fluorescent Protein Background In molecular biology, transformation refers to a form of genetic exchange in which the genetic material carried
More informationLAB 3 Use of the Microscope
LAB 3 Use of the Microscope Introduction In this laboratory you will be learning how to use one of the most important tools in biology the compound light microscope to view a variety of specimens. You
More informationENUMERATION OF MICROORGANISMS. To learn the different techniques used to count the number of microorganisms in a sample.
ENUMERATION OF MICROORGANISMS I. OBJECTIVES To learn the different techniques used to count the number of microorganisms in a sample. To be able to differentiate between different enumeration techniques
More informationUsing a Microscope to See Different Types of Cells
Using a Microscope to See Different Types of Cells copyright 2003 by Dr. Vivianne Nachmias, University of Pennsylvania All organisms are made up of cells - a cell is the simplest collection of matter that
More informationMicroscopy. MICROSCOPY Light Electron Tunnelling Atomic Force RESOLVE: => INCREASE CONTRAST BIODIVERSITY I BIOL1051 MAJOR FUNCTIONS OF MICROSCOPES
BIODIVERSITY I BIOL1051 Microscopy Professor Marc C. Lavoie marc.lavoie@cavehill.uwi.edu MAJOR FUNCTIONS OF MICROSCOPES MAGNIFY RESOLVE: => INCREASE CONTRAST Microscopy 1. Eyepieces 2. Diopter adjustment
More informationExercise V. Bacterial Cultural Characteristics or Morphology
Exercise V Bacterial Cultural Characteristics or Morphology When a single bacterial cell is deposited on a solid or in a liquid medium, it begins to divide. One cell produces two, two produce four, four
More informationProkaryotic and Eukaryotic Cells
Lab 2- Bio 201 Prokaryotic and Eukaryotic Cells Name: OBJECTIVES To explore cell structure and morphology in prokaryotes and eukaryotes. To gain more experience using the microscope, and in particular,
More informationChapter 4. Microscopy, Staining, and Classification. Lecture prepared by Mindy Miller-Kittrell North Carolina State University
Chapter 4 Microscopy, Staining, and Classification 2012 Pearson Education Inc. Lecture prepared by Mindy Miller-Kittrell North Carolina State University Microscopy and Staining 2012 Pearson Education Inc.
More informationGram Staining. The Most Commonly Used Differential Stain. Advantages:
Gram Staining The Most Commonly Used Differential Stain Advantages: Can observe size and morphology (like other staining) Can find out additional information about the organism- primarily what type of
More informationQuantifying Bacterial Concentration using a Calibrated Growth Curve
BTEC 4200 Lab 2. Quantifying Bacterial Concentration using a Calibrated Growth Curve Background and References Bacterial concentration can be measured by several methods, all of which you have studied
More informationBacterial Transformation with Green Fluorescent Protein. Table of Contents Fall 2012
Bacterial Transformation with Green Fluorescent Protein pglo Version Table of Contents Bacterial Transformation Introduction..1 Laboratory Exercise...3 Important Laboratory Practices 3 Protocol...... 4
More informationA Fishy Tale. Observing the Circulatory System of a Goldfish with a Compound Light Microscope
A Fishy Tale Observing the Circulatory System of a Goldfish with a Compound Light Microscope A Fishy Tale About this Lesson In this lesson, students will explore a computer animation of the human body
More informationLAB 4. Cultivation of Bacteria INTRODUCTION
LAB 4. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth
More informationMALARIA PARASITE COUNTING
VERSION 1 EFFECTIVE DATE: 01/01/2016 MALARIA PARASITE COUNTING MALARIA MICROSCOPY STANDARD OPERATING PROCEDURE MM-SOP-09 1. PURPOSE AND SCOPE To describe the procedure for counting malaria parasites on
More informationDisc Diffusion Susceptibility Methods
Disc Diffusion Susceptibility Methods Introduction When a filter paper disc impregnated with a chemical is placed on agar the chemical will diffuse from the disc into the agar. This diffusion will place
More informationUse of the Microscope and Cytology
Use of the Microscope and Cytology Introduction: A true study of anatomy not only considers the large, visible structures of an organism, but also the small structures that provide the organism its form
More informationPotato Microbiology. Sarah Follenweider, The English High School 2009 Summer Research Internship Program
Potato Microbiology Sarah Follenweider, The English High School 2009 Summer Research Internship Program Introduction: A number of microorganisms thrive on the nutrients that can be found in a potato. My
More informationHISTOLOGY LABORATORY. Microscope Orientation and Blood Smear Lab
HISTOLOGY LABORATORY Microscope Orientation and Blood Smear Lab For practicing how to use the microscope DO NOT use the blood smear slide (it is too boring for the lower mags). Use a slide from the white
More informationFirstView 3 Reflector Telescope Owner s Manual
FirstView 3 Reflector Telescope Owner s Manual 1. Horizontal Locking Auxiliary Screw 2. Main Mount 3. Pitching Auxiliary Knob 4. Pitching Shaft Screw 5. Rack and Pinion Focusing Knob 6. Thumb Nut for Finder
More informationNormal flora, which make up about 90% of the cells of a human body, are microbes that
Indigenous Unknowns Lab Report Introduction: Normal flora, which make up about 90% of the cells of a human body, are microbes that live and grow on the body without causing disease under normal conditions.
More informationRauserv Steam Sterilizers, Laboratory and Hospital Equipment. Brewery Laboratory Equipment
Rauserv Steam Sterilizers, Laboratory and Hospital Equipment Brewery Laboratory Equipment Date: 01 September 2015 Table of Contents 1. Anaerobic Jar... 1 2. Autoclave... 2 3. Balance... 3 4. DO (Dissolved
More informationAURAMINE O STAIN. Preanalytical Considerations
AURAMINE O STAIN Preanalytical Considerations I. PRINCIPLE Acid-fast mycobacteria resist decolorization by acid-alcohol after primary staining owing to the high lipid (mycolic acid) content in their cell
More informationURINE CULTURES GENERAL PROCEDURE
University of Nebraska Medical Center Division of Laboratory Science Clinical Laboratory Science Program CLS 418/CLS 419 URINE CULTURES GENERAL PROCEDURE I. Principle Urine cultures are performed to detect
More informationIsolation of Starch degrading bacteria Enzymes in Action
Isolation of Starch degrading bacteria Enzymes in Action Introduction In this laboratory exercise, you will be playing the role of biotechnologists in search of a new amylase. Since most industrially used
More informationNNIN Nanotechnology Education
NNIN Nanotechnology Education How Quickly Do Bacteria Grow? Teacher s Guide Purpose: Students will relate real-world applications to mathematical concepts by monitoring bacterial growth over one week and
More informationNational Optical & Scientific Instruments Inc. 11113 Landmark 35 Drive San Antonio, Texas 78233 Phone (210) 590-9010 Fax (210) 590-1104
National Optical & Scientific Instruments Inc. 11113 Landmark 35 Drive San Antonio, Texas 78233 Phone (210) 590-9010 Fax (210) 590-1104 INSTRUCTIONS FOR MODELS 106, 106-L 107, 107-L 108, 108-L 109-L ELEMENTARY
More informationRelated topics: Application Note 27 Data Analysis of Tube Formation Assays.
Tube Formation Assays in µ-slide Angiogenesis Related topics: Application Note 27 Data Analysis of Tube Formation Assays. Contents 1. General Information... 1 2. Material... 2 3. Work Flow Overview...
More informationSTAINING AND BACTERIAL CELL MORPHOLOGY. To learn the techniques of Gram staining, nigrosin staining and KOH test.
STAINING AND BACTERIAL CELL MORPHOLOGY I. OBJECTIVES To learn the technique of smear preparation. To learn the techniques of Gram staining, nigrosin staining and KOH test. To use and relate the Gram stain
More informationProtists and Fungi. What color are the cells in the living culture?
Protists and Fungi Objectives 1. Recognize and identify (to genus) the organisms covered in lab. 2. Describe the characteristics of each organism. 3. Correctly classify the organisms. I. Protists The protists
More informationGROWING BACTERIA INTRODUCTION
GROWING BACTERIA INTRODUCTION E. coli is a normal part of the bacterial flora of the human gut. It is not generally considered pathogenic, although some strains are highly toxic (recent food poisonings
More informationOBJECTIVES PROCEDURE. Lab 2- Bio 160. Name:
Lab 2- Bio 160 Name: Prokaryotic and Eukaryotic Cells OBJECTIVES To explore cell structure and morphology in prokaryotes and eukaryotes. To gain more experience using the microscope. To obtain a better
More informationUrinalysis and Body Fluids CRg
Urinalysis and Body Fluids CRg Unit 2; Session 1 Urine Microscopic Examination The Complete Urinalysis Physical properties already covered Chemical analysis in the next unit Microscopic our current focus
More informationLaboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus
Laboratory Exercise # 11: Differentiation of the Species Staphylococcus and Streptococcus Purpose: The purpose of this laboratory exercise is to explore the differences between Staphylococcal species and
More informationMODULE 2D ENVIRONMENTAL MICROBIOLOGICAL LABORATORY ACCREDITATION PROGRAM (EMLAP) ADDITIONAL REQUIREMENTS
MODULE 2D ENVIRONMENTAL MICROBIOLOGICAL LABORATORY ACCREDITATION PROGRAM (EMLAP) ADDITIONAL REQUIREMENTS 2D.1 SCOPE The AIHA- Laboratory Accreditation Programs (AIHA-LAP), LLC s Environmental Microbiological
More informationNational Optical & Scientific Instruments Inc. 11113 Landmark 35 Drive San Antonio, Texas 78233 Phone (210) 590-9010 Fax (210) 590-1104
National Optical & Scientific Instruments Inc. 11113 Landmark 35 Drive San Antonio, Texas 78233 Phone (210) 590-9010 Fax (210) 590-1104 INSTRUCTIONS FOR MODELS 156, 156-S, 157 COMPOUND BIOLOGICAL MICROSCOPES
More informationFirst let us consider microscopes. Human eyes are sensitive to radiation having wavelengths between
Optical Differences Between Telescopes and Microscopes Robert R. Pavlis, Girard, Kansas USA icroscopes and telescopes are optical instruments that are designed to permit observation of objects and details
More informationIDENTIFICATION OF UNKNOWN BACTERIA
IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features
More informationLecture Objectives: Why study microbiology? What is microbiology? Roots of microbiology
1 Lecture Objectives: Why study microbiology? What is microbiology? Roots of microbiology Why study microbiology? ENVIRONMENTAL MEDICAL APPLIED SCIENCE BASIC SCIENCE The science of microbiology Microbiology
More informationVISM Evolution Scope Series
1 VISM Evolution Scope Series Congratulations on the purchase of your New VISM Evolution (EVO) Series Scope! The EVO Series of Scopes give you many great high end features and various magnification ranges
More informationEnvironmental Monitoring
Environmental Monitoring Purpose of Environmental Monitoring Critical process within the pharmaceutical and biotechnology industries. Determines the microbial and particulate content of cleanroom air and
More informationIDENTIFICATION OF UNKNOWN BACTERIA
IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features
More informationMedical Microbiology Microscopic slides and media
Medical Microbiology Microscopic slides and media Head of Microbiology Department and Laboratory Medical Immunology : Janina Grzegorczyk MD, PhD, professor Implementators: Małgorzata Brauncajs MD Zbigniew
More informationLenses and Telescopes
A. Using single lenses to form images Lenses and Telescopes The simplest variety of telescope uses a single lens. The image is formed at the focus of the telescope, which is simply the focal plane of the
More informationCALIBRATION FOR LAL20X & LAL24X
CALIBRATION AND FAULT FINDING FOR LAL20X & LAL24X DUMPY LEVELS MEASURING EXPERTS SINCE 1869 How The LAL20X & LAL24X Works The Automatic level is called Automatic because it requires only a simple basic
More informationIntroduction. Materials. 104 Bacterial Identification
Chapter 8 Identification of acterial Species Kimberley Christopher and Elsa runo Department of iological Sciences University of lberta Edmonton, lberta, CND T6G 2E9 Kim.Christopher@ualberta.ca Elsa.runo@ualberta.ca
More informationTo measure an object length, note the number of divisions spanned by the object then multiply by the conversion factor for the magnification used.
STAGE MICROMETERS Introduction Whenever there is a need to make measurements with an eyepiece graticule, there is also a need to ensure that the microscope is calibrated. The use of a stage micrometer
More informationEXPERIMENT 6 OPTICS: FOCAL LENGTH OF A LENS
EXPERIMENT 6 OPTICS: FOCAL LENGTH OF A LENS The following website should be accessed before coming to class. Text reference: pp189-196 Optics Bench a) For convenience of discussion we assume that the light
More informationHow To Use An Asbestos Microscope
Asbestos Microscopes and Accessories Pyser-SGI has been supplying microscopes and accessories into Asbestos Laboratories for over 40 years PS12 Stage Micrometer with UKAS Certificate of Calibration - For
More informationAcknowledgements. Developing collaborative lab experiments across disciplines through the identification of bacteria
Acknowledgements Developing collaborative lab experiments across disciplines through the identification of bacteria Joanna Huxster, Ph.D. Sarah Moss, MS 15 Emily Bilyk, BS 16 Brian M. Forster, Ph.D. Lab
More informationTest Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces
Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa
More informationVIRTUAL EXPERIMENT 5A OXYGEN RELATIONSHIPS (REVISED FROM THE ON-LINE MANUAL)
VIRTUAL EXPERIMENT 5A OXYGEN RELATIONSHIPS (REVISED FROM THE ON-LINE MANUAL) One often sees an organism described as being a strict aerobe, facultative anaerobe, strict anaerobe or some other such designation.
More informationIsolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water
Iranian J. Publ. Health, Vol. 30, Nos. 3-4, PP. 91-94, 2001 Isolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water MK Sharifi-Yazdi 1,
More informationBUGS" THAT PRODUCE DRUGS TO KILL "BUGS Microbes Produce Antibiotics
BUGS" THAT PRODUCE DRUGS TO KILL "BUGS Microbes Produce Antibiotics Science in the Real World Microbes In Action BUGS" THAT PRODUCE DRUGS TO KILL "BUGS is a curriculum unit developed as part of the Science
More informationLab 10: Bacterial Transformation, part 2, DNA plasmid preps, Determining DNA Concentration and Purity
Lab 10: Bacterial Transformation, part 2, DNA plasmid preps, Determining DNA Concentration and Purity Today you analyze the results of your bacterial transformation from last week and determine the efficiency
More information14 The ability of the lenses to distinguish fine detail and structure is called a. Illumination b. Magnification c. Refractive index d.
1 2 Assume you stain Bacillus by applying malachite green with heat and then counterstain with safranin. Through the microscope, the green structures are a. cell walls. b. capsules. c. endospores. d. flagella.
More informationTransformation Kit BACTERIAL TRANSFORMATION: GREEN FLUORESCENT PROTEIN. Partnership for Biotechnology and Genomics Education
Transformation Kit BACTERIAL TRANSFORMATION: GREEN FLUORESCENT PROTEIN Partnership for Biotechnology and Genomics Education Barbara Soots Linda Curro Education Coordinator University of California Davis
More informationPlaneWave CDK Telescope Instructions CDK12.5, 17, 20 and 24
PlaneWave CDK Telescope Instructions CDK12.5, 17, 20 and 24 V112712 1 Collimation and Secondary Spacing Procedure The CDK optical design has four optical elements shown in Figure 1. The primary mirror
More informationProtocol for Microscope Calibration
Protocol for Microscope Calibration A properly calibrated system is essential for successful and efficient software use. The following are step by step instructions on how to calibrate the hardware using
More informationThe illustrations below reflect other scientists results in identifying and counting the stages of the onion root tip and the whitefish blastula.
Abstract: The purpose of this laboratory experiment was to identify in what stage of mitosis viewed cells were in. The stages of mitosis include prophase, metaphase, anaphase and telophase. Although the
More informationVISM CQB Scope Series
1 VISM CQB Scope Series Congratulations on the purchase of your new VISM CQB Scope! The CQB Series of Scopes give you many great options so you can choose the scope that best fits your needs. Backed by
More informationGeometric Optics Converging Lenses and Mirrors Physics Lab IV
Objective Geometric Optics Converging Lenses and Mirrors Physics Lab IV In this set of lab exercises, the basic properties geometric optics concerning converging lenses and mirrors will be explored. The
More informationBydureon 2 mg powder and solvent for prolonged-release suspension for injection in pre-filled pen How to use Bydureon pre-filled pen
INSTRUCTIONS FOR THE USER Read these instructions carefully before use Bydureon 2 mg powder and solvent for prolonged-release suspension for injection in pre-filled pen How to use Bydureon pre-filled pen
More informationCOMPARING PLANT AND ANIMAL CELLS
COMPARING PLANT AND ANIMAL CELLS OBJECTIVES: Distinguish between plant and animals cells by their structures Demonstrate the benefit of stains Acquire ability to prepare wet mounts SAFETY: Methylene blue
More informationFigure 1. Basic structure of the leaf, with a close up of the leaf surface showing Stomata and Guard cells.
BIOL100 Laboratory Assignment 3: Analysis of Stomata Name: Stomata (singular=stoma) are the respiratory control structures in plants (see Figure 1 below). They are essentially small holes in the surface
More informationStandard Operating Procedure (SOP)
Standard Operating Procedure (SOP) Title: Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues Number: BACT-3 Version: 02 Created December 14, 2011 Approval:
More informationMicrobiological Testing of the Sawyer Mini Filter. 16 December 2013. Summary
Microbiological Testing of the Sawyer Mini Filter 16 December 2013 Summary The Sawyer Mini Filter was tested for its ability to remove three microorganisms Raoultella terrigena, Bacillus subtilis, and
More informationSAMPLE. Bacterial Transformation. Lab 8 BACKGROUND INFORMATION. Neo/SCI Student s Guide Name... Teacher/Section...
1431489 REV 001 Neo/SCI Lab 8 Bacterial Transformation BACKGROUND INFORMATION What Is Biotechnology? Before you start doing biotechnology laboratory exercises, it is important to know exactly what biotechnology
More informationDiagnostic Techniques: Urine Culture
Diagnostic Techniques: Urine Culture Melissa Schreiber, Presenter: Valencia College, Orlando, FL mschreiber@valenciacollege.edu Objectives: After completing this project the students should be able to:
More information`TORAY TEXTILES EUROPE
`TORAY TEXTILES EUROPE See It Safe ANTI-MICROBIAL TEST RESULTS October 2006 Antimicrobial Test Methods Used ' TORAY' AATCC 147 (Modified) Antibacterial Activity Assessment of Tetile Materials: Parallel
More information