Bioer Real-Time PCR Detection Systems

Transcription

1 Bioer Real-Time PCR Detection Systems LineGene 9600 qpcr Performance with Ease Flexibility Reliability

2 About Bioer Technology Company Bioer Technology Company (Bioer) evolved from the R&D department of Ferrotec China, one of the largest high-quality Peltier suppliers in the world. This highly professional enterprise develops, manufactures and markets a first-class range of PCR related instruments and reagents. Its extensive state-of-the-art facility in Hangzhou, China, occupies 26,000 m 2 and includes a 10,000 class cleanroom workshop area, GMP reagent workshop and laboratory centre. Bioer manufactured the first thermal cyclers in China in 1998, and its first real time instruments in Since then the company has placed more than 1000 units in the Asian market and won many awards and significant recognition. Bioer is rapidly becoming an internationally recognised brand with distributors in the US, Germany, Japan, South Korea, South East Asia, South America, the Middle East and the UK. Developing Real-Time Real-time PCR provides fast, precise, sensitive detection and is widely used in clinical diagnostics, research, teaching, food quarantine, disease prevention etc. The LineGene series of instruments, developed and manufactured by Bioer were the first Real-Time PCR systems based on Peltier technology in China. They are also the first to be registered and approved by the SFDA (State Food and Drug Administration). These thermal cyclers are programmable, feature rapid temperature change, accurate temperature control and can automatically record and analyze the data after amplification. Bioer has continued to progress and innovate its range and now introduces the LineGene This latest product in the Bioer real-time PCR detection system family is its first 96 well based system, with up to 8 detection channels and a greater temperature range than previous models. The unique patented block dissipation technology provide the LineGene 9600 with better heating and cooling rates and improved temperature accuracy, uniformity and stability. The LineGene 9600 uses dual colour scanning covering the whole fluorescence detection wavelength, to collect data from a 96 well plate in just over 5 seconds.

3 LineGene 9600 Real-Time Thermal Cyclers The LineGene 9600 series of instruments are 96 well Real-Time PCR systems capable of performing Absolute and Relative Quantification together with SNP and melt curve analysis. The LineGene 9600 is compatible with SBS footprint PCR plates, 8 or 12 strip-tubes and single 0.2ml tubes. This provides greater flexibility whether you are performing high throughput or low throughput applications. Being more versatile than high throughput specific systems, the LineGene 9600 series are ideally suited to meet the needs of any laboratory. The LineGene 9600 systems are equipped with gradient functionality for rapid optimisation, and have a melting temperature calculator useful for establishing protocols. There is a choice of block or tube temperature regulation. The latter uses an internal algorithm which calculates the temperature of the actual sample in real time during the PCR process. The hot-lid temperature can be set as required and the height adjusts automatically to the size of the tube or plate, effectively preventing evaporation. Key Benefits Performance Ramping rate 4C/second - shorter reaction times, faster results Detection range copies - sensitive detection for low copy numbers Real-time fast double wavelength X-Y scan system - enhancing overall speed High sensitivity PMT (photomultiplier tube) x 2 High stability wide range, low temperature drift Patented block dissipation technology - providing uniform temperature across the block Unique Bottom Detection Method with specially designed Peltiers, helps to maximise signal detection, minimise crosstalk and provide more reproducible results High light long life LED - maintenance free, small emission angle and high brightness

4 Technical Specifications Temperature This instrument is fitted with a peltier unit for thermal cycling with a temperature range of 4-105C. The incorporated patented block dissipation technology produces a ramping rate of 4C/second, allowing a run of 40 cycles to be completed in just over 60 minutes. The temperature uniformity of this block is on average ±0.3C and has temperature control accuracy of ±0.1C and a temperature fluctuation of ±0.1C. The block also has gradient functionality with a temperature span of 36C. Optics Excitation The LineGene 9600 series use long-lasting LEDs as the light source for the excitation of the individual fluorescence channels. LEDs work with very low energy and are stable in intensity and wavelength. Compared to other types of illumination they have a very long lifespan. System maintenance and calibration is minimised by the use of optical filter sets. There are 4 models available in the LineGene 9600 range with 2, 4, 6 or 8 filter channels, which are capable of detecting the following dyes: Filter channel 1: FAM, SYBR green 1 Filter channel 2: VIC, HEX, TET, JOE Filter channel 3: CY3, NED, TAMRA Filter channel 4: ROX, TEXAS RED Filter channel 5: Cy5 Filter channel 6: Lightcycler Red Filter channel 7: Custom Filter channel 8: Custom

5 Detection Photo-multiplier tubes (PMT s) are capable of detecting a single photon of light and demonstrate higher performance than CCD or photodiode systems. The LineGene 9600 series uses a unique dual photomultiplier detection system which measures the fluorescence signal from the bottom of the well to the top. This maximises signal detection and minimises well to well cross talk. Chemistry These open platform systems can be used with many different qpcr chemistries including hydrolysis probes and SYBR green based chemistries. Operation The LineGene 9600 systems can be run using a RS232C device, a USB device or an optional Bluetooth function connecting the instrument to a PC. The instrument can be run on Windows 2000/XP/Vista/7. Flexibility Capacity: 96 well plate format, 8 or 12 strip tubes and 0.2ml tubes Reaction volumes 5-100μl Open platform to allow use with different chemistries Up to 8 Wavelength Optical filters (2 can be customized) Instrument capability - SNP Genotyping - Absolute Quantification - Relative Quantification - Melt Curve Analysis Gradient function Multiplexing Auto adjustable pressurised hot-lid

6 Set up with Ease and Speed Software The LineGene 9600 series Research Software is included with the system. This easy-to-use intuitive software enables experiments to be set up with ease and analysed with minimal effort. It can be used for Absolute Quantification using a standard curve, Relative Quantification of different genes, together with Endpoint Genotyping analysis by detection of SNPs. Simple navigation and clearly displayed windows guide you through 4 steps for rapid reaction set up: Detector Sample Plate Programme Options to use recently opened experiments and to import experiment files further enhance usability.

7 Absolute, Relative or SNP The system window consists of the Menu bar, the Tool bar and the Main Form. Absolute, Relative or SNP Experiment type selection Recently opened files Categorised templates Detector Input experiment name, user name and any comments in the experiment property column. Detector Add, modify and delete detectors and assays in the detector library Detector Assay Dye name Colour Sample set up Add and delete sample information individually or by import/export. Batch add function is also available. Sample set up

8 Analyse with Ease and Speed User-friendly graphic programming, simple operation and practical. Temperature gradients can easily be determind using the Gradient Temperature calculator. The system has a gain calibration function enabling Auto gain or Manual settings. Analysis Data can be viewed and analysed quickly using the amplification plots, scatter plots, standard curves, auto call features and reporting options. Fluorescent and temperature curves are displayed in real-time, and conventional methods such as setting a threshold, linear regression or via second derivation are used to determine the Cq values. (Cq = quantification cycle, sometimes referred to as Ct = threshold cycle). Fluorescent curves can be shown in linear or logarithmic form as desired. The integrated quality system indicates if your results and controls are consistent with pre-defined parameters. The data can be exported to a.csv file, alternatively a customised report can be created.

9 Amplification Curve Example of a typical SYBR Green I amplification curve. The fluorescence signals of 96 samples are detected in each PCR cycle and shown in the graph. Baseline threshold, the maximum second derivative method and linreg3 methods are used to analyse the amplification curve of exponential growth of the start point, end point and closest point of efficiency, a key to automatically complete the analysis. The Analysis tab guides you through the data plots. The data can be further manipulated in Microsoft Excel. The reporting options can be customised to include appropriate data sets and other critical information. The reports can also be printed or easily exported into Microsoft Powerpoint. SNP Analysis Relative Analysis Melting Curve Post-amplification melting curve analysis is a simple way to check for specificity. Melting curve The graph shows the change in fluorescence plotted against the change in temperature, to provide a clear view of the melting dynamics.

10 Performance Excellent Uniformity HBV templates diluted to copies/reaction amplified in the presence of a FAM-labelled detection probe. Graph shows 96 replicates. Wide Detection Range Genomic DNA templates diluted 10-fold (between copies) and amplified using a FAM reporter dye.

11 LineGene 9600 Real-Time Thermal Cycler Specifications and Ordering Information Model Cat. No. TC5020 TC5010 TC5000 TC5030 Sample Capacity 96 well microplate, 12 x 8 strip tubes, 96 x 0.2ml tubes (bottom transparent) Excitation wavelength nm Emission wavelength nm Detected fluorescence F1:FAM, SYBR Green I; F2:VIC, HEX, TET, JOE; F1:FAM, SYBR Green I; F2:VIC, HEX, TET, JOE; F3:CY3, NED, TAMRA; F4:ROX, TEXAS-RED F1:FAM, SYBR Green I; F2:VIC, HEX, TET, JOE; F3:CY3, NED, TAMRA; F4:ROX, TEXAS-RED; F5:CY5; F6:LightCycler Red F1:FAM, SYBR Green I; F2:VIC, HEX, TET, JOE; F3:CY3, NED, TAMRA; F4:ROX, TEXAS-RED; F5:CY5; F6:LightCycler Red; F7 & F8 can be customised Block temperature range C (minimum increments 0.1 C) Heating/cooling rate 4 C/sec Temperature control accuracy ± 0.1 C Temperature fluctuation ± 0.1 C Temperature uniformity ± 0.3 C Temperature control mode Block or tube simulation mode (automatic control based on sample volume) Sample volume range 5-100µl Gradient temperature range 1-36 C Hot-Lid temperature range 30 C to 110 C (adjustable, default 105 C, Automatic) Fluorescence detection repeatability 5% Scan mode Entire plate or dedicated line Programme Maximum 20 segments for each programme, Maximum 99 cycles Operation Mode Continuous Scan period 5.5s (F1:F2 plate scan) Functionality Absolute & relative quantification, SNP detection and melt curve analysis Operation system Microsoft: Windows 2000/XP/vista/windows 7 Software: Excel 2000/2003/2007; Access 2000/2002/2007 PC configuration Memory:512M, Hard Disk:10GB, CPU:Pentium 4, Virtual memory: 1000MB Power supply V, ~50/60Hz, 600W Dimensions (LxWxH) 43cm x 39.5cm x 35.2cm Net weight 28Kg Connectivity RS232C adapter, USB adapter (optional), Blue Tooth adapter (optional) Cat No Product Description List Price TC5020 LineGene9620, 2 channel QPCR Machine 18,000 TC5010 LineGene9640, 4 channel QPCR Machine 19,500 TC5000 LineGene9660, 6 channel QPCR Machine 22,500 TC5030 LineGene9660, 8 channel QPCR Machine On request Accessories and companion products Cat No Product Description Pack Size List Price LW Well Unskirted PCR Plate, Nat 10x LW2786 ThermalSeal RT2 1x LW ml 8-Strip Tubes & Ind Caps Natural Special prices are available when purchased with a LineGene instrument. Please enquire for further details and pricing or visit:

12 LineGene 9600 Real-Time PCR Detection System For more information visit: Alpha Laboratories Ltd 40 Parham Drive Hampshire SO50 4NU Tel: +44 (0) Web: UIN111A 04.14