Micronutrients, Flavors and Herbs

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1 Value addition to Tea with Micronutrients, Flavors and Herbs ILSI 2011 Dr Gautam Banerjee Expertise Area Director-Nutrition and Health Unilever R&D D, Bangalore, India

2 History Tells Us About The Health Benefits Of Tea One of England s greatest PM William Gladstone: "If you are cold, tea will warm you. If you are hot, it will cool you. If you are depressed, it will cheer you. If you are excited, it will calm you.

3 The Benefits of Tea Feeling Good Now, Tomorrow and Beyond Immediate Years Healthy Heart Body Shape Pleasure (Taste) Lifts Spirits (Aroma) Thirst Quenching Refreshing Energy Metabolism Immune Benefits Skin Radiance < One Hour Gentle Boost (Physical & Mental) Relaxed and Alert Body Hydration Weeks/ Months

4 Tea as Carrier of Goodies

5 Why Fortify tea? Consumed by large section of population 96% penetration of beverage among adults 88% penetration among children above 5 years of age Affordable to the target t population Does not involve any change in lifestyle Tea is a part of everyday life in most part of the Globe. 2-3 cups of tea consumed per day

6 Trends: Functional Tea Beauty benefit Energizing & Relaxing Digestive Immunity Boost Improving Cardiovascular Health Low sugar

7 Tea : Fortified with Micronutrients Why fortify with vitamins? Known deficiency/ intake gaps for vitamins in the population Technical feasibility of adding the vitamins Stability of vitamins in the food

8 Flavored Tea Active Life Drinks : Health & Energy and Youth & Vitality, with green tea, ginkgo biloba, vitamin C and biotin.

9 Tea: Fortified with Herbs

10 CASE STUDY

11 Immunity Benefit Through Tea Fortified with Herbs

12 The Paradigm Immune function is key for resistance to infections, but can be compromised by various factors allergens bac cteria virus par rasites cance cer cells immune system malnutrition stress ageing

13 The Approach Leads from Ayurveda - Traditional Indian System of Medicine. - Provides Good to excellent leads for various health benefit. - Retains the health of healthy & prevents disease. - Improves the health of unhealthy. Boosting of Natural Immunity

14 Human Innate Immune System NK cell is a validated marker for IL-12 Type 1 IFN Natural Immunity NK Cell

15

16 Cell Culture Based Bio-Assays 4.00 Natural Killer Cell Assay Would it work in Human??? Control Ginger Basil M ulethi Ashwagandha Natural Killer cell activity enhanced by herbs in vitro.

17 The Herbs Liquorice Ashwagandha Glycyrrhiza glabra Withania somnifera Holy Basil Ginger Ocimum sanctum Zingiber officinale

18 Herb Specific Markers Herbs Ocimum sanctum. Withania somnifera... Glycyrrhiza y glabra Zingiber officinale. Marker molecule Ursolic acid Withaferin A Glycyrrhetinic acid 6-Gingerol Gingerol

19 Detection of Herbs in Tea Extract Gingerol, λmax :320nm, λmax :355nm Detection wavelength: 282nm Glycyrrhetinic acid, Detection λmax: 276nm wavelength: 250nm Retention time: 5.64 min Retention time: 8.48 min 6-Gingerol Withaferin A, λmax 228nm Detection wavelength: 250nm Ursolic acid, λmax: 200nm Detection wavelength: 210nm Retention time: min Retention time: 7.42 min

20 Proof of Efficacy Human Efficacy Trials: (2 trials) - First trial (n=32, 2 months, parallel)- Mumbai (3 cups of tea per day). - Second trial (n=100, 4.5 months, cross over)- Bangalore (3 cups of tea per day). Study End Points: Study End Points: Natural killer cell (NK cell) activity

21 Result (Trial I) ity (%) NK Cell Activ Regular Tea * Fortified Tea Intervention Duration (Months) Consumption of Fortified tea significantly improves NK cell activity.

22 Result (Trial - II) Regular Tea 0 month 2 months (6.6 ± 0.5) (19.3±2.1) Regular Tea 2 months 4 months 2.5 months 5 months 2.1) (26.7±2.8) 2.8) (15.41±2.23) (15.32± 2.22) Wash off 15 days (6.8±0.6) 06) (285± (28.5± 2.8) (29.99±2.8) 99±2.8) 28) (17.58± ) 178) (13.73±2.82) 0 month 2 months 4 months 2.5 months 5 months Fortified Tea Fortified Tea Consumption of fortified tea significantly improves NK cell activity.

23 Summary Tea is a zero calorie healthy beverage. Successfully used to deliver nutrients. Good carrier for herbs/ vitamins/other goodies for various health benefits.

24 Questions & Answers THANK YOU

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