Training Programme in. Assisted Reproductive Technologies:

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1 Training Programme in Assisted Reproductive Technologies: In vitro Fertilisation & Embryo Transfer (IVF-ET) and Intra-Cytoplasmic Sperm Injection (ICSI)

2 Published By: Trivector Embryo Support Academy (TESA) A division of Trivector Scientific International Marathon Max, Hedgewar Chowk, LBS Marg, Mulund (W) Mumbai Location of the Training School: 104-Park Royale, Madan Malviya Road, Off L.B.S Marg, Mulund (w) Mumbai Trivector Embryo Support Academy No part of this publication can be reprinted in any form without the prior permission of the authors. This publication is for private circulation only. It is meant as a support to the presentations and practical training sessions held during the training programmes. Published in

3 Trivector Scientific International has been regularly conducting training programmes on the Assisted Reproductive Technologies initially under the aegis s of the Trivector Fertility School in collaboration with the Medicult Fertility School, Denmark and now under its own Academy - TESA. These training programmes are aimed to give a complete insight into the various aspects of Assisted Reproductive Technologies with a greater emphasis on the setting up, maintenance and the techniques involved in Assisted Reproductive Technologies (ART). The participants to these programmes are either clinicians (gynecologists, physicians or andrologists) or potential embryologists (with at least a Bachelor s degree in any of the Biological Sciences). These intensive training programmes include a series of lectures on the basics of reproductive biology; the science behind ART; the principles behind each of the techniques of ART; demonstrations of the ART; long hours of hands-on experience on each step of ART including Intra-Cytoplasmic Sperm Injection (ICSI). Demonstration of the newer technologies such as laser assisted hatching as well as Intra-cytoplasmic Morphologically Selected Sperm Injection (IMSI). Faculty: Dr. Rajvi H Mehta PhD: She is one of the first doctorates in the field of human IVF-ET in India having started her career under the tutelage of Drs. T.C Anand Kumar and Dr Indira Hinduja, who were responsible for the birth of India s first scientifically documented IVF baby in Since then she has been very active in the field including training and research. Ms. Cheryl Mascerenhas MSc: She has completed a three years Masters in Clinical Embryology from the Manipal Institute of Technology. She is herself very skilled in the ART with extreme patience necessary for the practical training. The faculty is ably supported by the engineers of Trivector Scientific International. 3

4 Contents Programme Presentations 1. Basics of Folliculogenesis and Oogenesis 2. Ovarian Stimulation Protocols 3. Follicular Aspiration 4. Screening, Grading and Denudation of oocytes 5. Assessment of Oocytes 6. Spermatogenesis and Evaluation of Semen 7. Sperm Egg interaction and Fertilisation 8. In Vitro Culture of embryos 9. Grading and selection of Embryos 10. Embryo Transfer 11. Principles of Cryopreservation 12. Laws and Guidelines on ART The ART Laboratory 1. List of Equipment 2. Maintenance of ART Laboratory / Equipment 3. Assessing Sterility in an ART Laboratory 4. Preparation of Workstations for ART 5. Work Culture in ART Laboratory Practical Protocols 1. Semen Analysis 2. Processing of Semen 3. Follicular Aspiration: Screening for oocytes and Egg Collection 4. Insemination 5. Denudation of oocytes 6. Intracytoplasmic Sperm Injection : Preparation 7. Immobilisation of Sperm 8. ICSI 9. Fertilisation Check and Embryo Culture 10. Embryo Transfer 11. Cryopreservation of Semen 12. Cryopreservation of Embryos Slow Freezing 13. Vitrification 14. Sperm Selection Techniques 15. Summary of Preparations for IVF ICSI 4

5 Programme Day 1: am Introduction to the Participants, Faculty and the Programme am Introduction to ART : Historical Perspectives am Basic Requirements for an IVF Laboratory: Infrastructure & Equipment pm Maintenance of ART Laboratory and Equipment pm Basics of Folliculogenesis and Oogenesis pm Lunch pm Ovarian Stimulation Protocols pm Follicular Aspiration pm Screening, Grading and Denudation of Oocytes pm Practical: Handling of Oocytes Day 2: am Spermatogenesis & Semen Evaluation am Practical: Semen Analysis am Semen Processing pm Practical: Semen Processing pm Lunch pm Sperm Egg Interaction and Fertilisation pm In Vitro Culture Techniques pm Practical: Preparation of dishes for Insemination & Insemination Day 3: am In vitro Culture of embryos & Grading of Embryos am Practical: In Vitro Culture Techniques am Grading of Embryo and Embryo Transfer pm Practical: Embryo Transfer pm Lunch pm Principles of Cryopreservation, Cryopreservation of Embryos pm Practical Demonstration : Cryopreservation of Embryos pm Practical Preparation of dishes for ICSI 5

6 pm Practical Understanding the ICSI Equipment Practical Demonstration: Laser Assisted hatching Day 4: am Practical Demonstration: Immobilisation of Sperm pm Practical Batch 1: Cryopreservation: Vitrification Batch 2: Cryopreservation of embryos: Slow Freezing Batch 3: Sperm Immobilisation pm Lunch pm Practical Demonstration ICSI pm Practical Batch 1: Sperm Immobilisation Batch 2: Cryopreservation: Vitrification Batch 3: Cryopreservation of embryos: Slow Freezing Day 5: pm Practical Batch 1: Cryopreservation of embryos: Slow Freezing Batch 2: Sperm Immobilisation Batch 3: Cryopreservation: Vitrification / ICSI pm Lunch pm Practical: ICSI pm ICMR ART Guidelines pm Quiz Trouble Shooting in ART pm Photo Shoot Certificate Distribution 6

7 ... Presentations 7

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