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DISCLOSURES CATEGORY Employment Research support Scientific advisory board Consultancy Speakers bureau Major stockholder Patents Honoraria Travel support Other CONFLICT No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose No conflict of interest to disclose

A Look at Antithrombotic Therapy and Novel Anticoagulants Anne Greist, MD Indiana Hemophilia & Thrombosis Center Indianapolis, IN 1-877-CLOTTER

OBJECTIVES Review the pharmacology of the novel (target specific) oral anticoagulants Explore their indications and use Discuss monitoring, management of bleeding, reversal

INCIDENCE OF VTE Adults 300,000 600,000 cases / year DVT 2.5-5% of general population 100,000-200,000 deaths / year PE Venous thromboembolism third most common CV disease 1. The Surgeon General's Call to Action to Prevent Deep Vein Thrombosis and Pulmonary Embolism. Office of the Surgeon General (US); National Heart, Lung, and Blood Institute (US). Rockville (MD): Office of the Surgeon General (US); 2008. 2. Beckman M G et al. Am J Prev Med 2010. 38(4 Suppl): S495-501.

RISK FACTORS FOR VENOUS THROMBOSIS ACQUIRED INHERITED MIXED/UNKNOWN Advancing age Factor V Leiden (FVL) Homocysteine Obesity Prothrombin G20210A Factor VIII Prior thrombosis Protein C deficiency APC resistance Immobilization Protein S deficiency Factor IX Major surgery Antithrombin deficiency Factor XI Malignancy Dysfibrinogenemias (rare) Free TFPI Estrogens Antiphospholipid antibody syndrome Myeloproliferative disorders, Malignancy, IBD, Nephrotic syndrome, Heparin induced thrombocytopenia Heit JA. J Thromb Haemost. 2005 Aug;3(8):1611-7. Review.

ATRIAL FIBRILLATION Most common sustained cardiac arrhythmia Fivefold increased risk of stroke Oral anticoagulation is treatment of choice for patients with CHAD₂ score =/>2 May be indicated for CHAD₂ score = 1 VKA prevent stroke with a RR reduction of 65% compared with placebo 1. CHEST 2012; 141(2)(Suppl):e531S e575s 2. Chai-Adisaksopha C et al. Blood 2014;124(15):2450-2458.

DISADVANTAGES OF VKAS Slow onset and offset of action Need for INR monitoring Interactions with diet and many other drugs Small therapeutic window Bleeding complications: 1.5-5.2% per year Chai-Adisaksopha C et al. Blood 2014;124(15):2450-2458.

TARGET-SPECIFIC ORAL ANTICAOGULANTS Directly inhibit factor Xa: - rivaroxaban - apixaban - edoxaban - (betrixaban, darexaban) Directly inhibits thrombin: - dabigatran Chai-Adisaksopha C et al. Blood 2014;124(15):2450-2458.

XII XIIa XI IX XIa IXa VIIIa VIIa Tissue Factor VII X Xa Va X II IIa Fibrinogen Fibrin Clot

XII XIIa XI IX XIa IXa VIIIa VIIa Tissue Factor VII X Xa Va X II IIa Fibrinogen Fibrin Clot

XII XIIa XI IX XIa IXa VIIIa VIIa Tissue Factor VII X Xa Va X II IIa Fibrinogen Fibrin Clot

XII XIIa XI IX XIa IXa VIIIa VIIa Tissue Factor VII X Xa Va X Dabigatran Rivaroxaban Apixaban Edoxaban II IIa Fibrinogen Fibrin Clot

NOVEL ORAL ANTICOAGULANTS Directly inhibit either Xa or IIa without requiring antithrombin All heparinoids and pentasaccharides require antithrombin to function Do not require routine monitoring of levels No reliable method to reverse critical bleeding Short half-life so missed doses result in lack of protection

COMPARISON OF RR Event Dabigatran Rivaroxaban Apixaban Embolism/stroke 0.77 (0.61-0.99) 0.93 (0.74-1.16) 0.96 (0.77-1.20) VTE 1.1 (0.66-1.84) 0.70 (0.46-1.07) 1.13 (0.76-1.69) Intracranial hemorrhage 0.26 (0.14-0.5) 0.58 (0.37-0.92) 0.51 (0.35-0.75) GI bleeding 1.5 (0.99-2.28) 1.46 (1.19-1.78) 0.88 (0.68-1.14) Major bleeding 0.90 (0.60-1.37) 1.03 (0.89-1.18) 0.70 (0.61-0.81) * RR all relative to warfarin = 1.0 Adam SS, McDuffie JR, et al. Ann Intern Med 2012;157:796.

1. Pradaxa Prescribing Information. Boehringer Ingelheim Pharmaceuticals. Updated 04/2014. 2. Lexi-Comp Online, Lexi-drugs. Hudson, Ohio: Lexi-Comp, Inc.; 2014. 3. Crowther M et al. Arterioscler Thromb Vasc Biol. 2015; 35:1736-1745 DABIGATRAN Avoid if CrCl <30 Use 75 mg bid if CrCl 15-30 Food delays but does not reduce absorption Affects TT>PTT>PT 85% renal clearance T 1/2 : 12-17h; mild-moderate CKD 15-18h; severe CKD 28h Protein binding 35%

FACTOR XA INHIBITORS Rivaroxaban Approved for VTE, AF and VTE prophylaxis Take with food Affects anti-xa>pt>ptt 66% renal clearance VTE: Avoid if CrCl <30 ml/min AF: 15 mg daily if CrCl 15-50 T 1/2 : 5-9h or 11-13h if elderly Not influenced by extremes of body weight Apixaban Approved for VTE, AF and VTE prophylaxis Not affected by food Affects anti-xa levels 27% renal clearance Reduce dose if 2 of: age >80, creatinine >1.5, weight <60 kg T 1/2 : ~12h 1. Eliquis Prescribing Information. Bristol-Meyers Squibb. Updated 03/2014. 2. Xarelto Prescribing Information. Janssen Pharmaceuticals. Updated 03/2014. 3. Lexi-Comp Online, Lexi-drugs. Hudson, Ohio: Lexi-Comp, Inc.; 2014.

PHARMACOKINETICS Warfarin Dabigatran Apixaban Rivaroxaban Edoxaban Bioavailability 100% 6.5% 50% 80-100% 60% Max concentration 4h 0.5-2h 3-4h 2-4h 1.5h Half-life 20-60h 12-14h 12h 11-13h 5-9h (young) 10-14h Renal clearance Protein binding 0% 85% 27% 66% 33% 99% 35% 87% 92-95% 40-59% Crowther M et al. Arterioscler Thromb Vasc Biol. 2015; 35:1736-1745

DOSING CONSIDERATIONS Condition Dabigatran Rivaroxaban Apixaban Liver disease Use caution Contraindicated if Child s B or C CrCl 15-30 Adjust dose if used for AF (contraindicated in Canada) Not recommended Caution if Child s B; Contraindicated if Child s C Caution* CrCl <15 Contraindicated Contraindicated Not recommended ǂ Pregnancy Lactation Category C; not recommended Unknown if excreted in breast milk; not recommended Category C Unknown if excreted Category B; still not recommended Unknown if excreted; not recommended * Reduce dose if at least 2 are true: creatinine 1.5, age 80 years, or weight <60 kg. ǂ Label approves use in ESRD, but American Heart Association recommends against use.

SELECTED DRUG INTERACTIONS Antacids Medications Dabigatran Rivaroxaban Apixaban Azole antifungals (excludes fluconazole) Not recommended, May reduce absorption Not recommended Avoid combination Avoid combination HIV/HCV antivirals Monitor therapy Avoid combination Avoid combination Rifamycins Avoid combination Avoid combination Avoid combination Quinidine Not recommended Monitor therapy Monitor therapy Dronedarone Not recommended Avoid combination Monitor therapy Amiodarone Clarithromycin Consider alternative, monitor therapy May increase levels, monitor therapy Monitor therapy May increase levels, monitor therapy Monitor therapy Avoid combination Lexi-Comp Online, Lexi-Interact. Hudson, Ohio: Lexi-Comp, Inc.; 2014.

HOW TO ADDRESS BLEEDING IN PATIENTS TAKING TARGET SPECIFIC ORAL ANTICOAGULANTS PUTTING THE BRAKES ON

DRUG MONITORING PT aptt TT Dilute TT Anti-Xa Dabigatran +/- NA Rivaroxaban +/- NA NA Apixaban +/- NA NA Edoxaban Unclear Unclear Unclear +/- May increase or produce no change. Reagent and individual dependent. Variable Increase Predictable increase that may be calibrated Highly sensitive increase, often exceeding upper limit of assay 1. Siegal DM, Garcia DA, et al. Blood 2014;123:1152. 2. Crowther M et al. Arterioscler Thromb Vasc Biol. 2015; 35:1736-1745

Procoagulants Measured by Screening Tests APTT sensitive to intrinsic and common pathway factors or inhibition of the coagulation process PT sensitive to extrinsic and common pathway factors or inhibition of the coagulation process TT sensitive to quantity and quality of fibrinogen or inhibition of the fibrin clot formation

Kearon C et al American College of Chest Physicians. Chest. 2012 Feb;141(2 Suppl):e419S-94S. Erratum in: Chest. 2012 Dec;142(6):1698-1704. Adapted from Table 2 [Section 2.3, 3] pg 2432S. RISK FACTORS FOR BLEEDING WITH ANTICOAGULANT THERAPY & ESTIMATED RISK OF MAJOR BLEEDING IN LOW-, MODERATE-, & HIGH-RISK CATEGORIES RISK FACTORS Age > 65 years Age >75 years Previous bleeding Cancer Metastatic cancer Renal failure Liver failure Thrombocytopenia Previous stroke Diabetes Anemia Antiplatelet therapy Poor anticoagulant control Comorbidity & reduced functional capacity Recent surgery Frequent falls Alcohol use ESTIMATED ABSOLUTE RISK OF MAJOR BLEEDING % CATEGORIZATION OF RISK OF BLEEDING LOW RISK (0 RISK FACTORS) MODERATE RISK (1 RISK FACTOR) HIGH RISK (> 2 RISK FACTORS) ANTICOAGULATION 0-3 MONTHS Baseline risk (%) 0.6 1.2 4.8 Increased risk (%) 1.0 2.0 8.0 Total risk (%) 1.6 3.2 12.8 ANTICOAGULATION AFTER FIRST 3 MONTHS Baseline risk (% per year) 0.3 0.6 > 2.5 Increased risk (% per year) 0.5 1.0 > 4.0 Total risk (% per year) 0.8 1.6 > 6.5

ANTICOAGULATION REVERSAL Blocking absorption Removing the circulating drug Increasing excretion Inactivating the drug Increasing the targeted clotting factor Promoting coagulation

ANTICOAGULATION REVERSAL Increasing the target - PCC: Kcentra 50 units/kg - apcc: FEIBA 50 units/kg Promoting coagulation - rfviia: NovoSeven 90 mcg/kg

SPECIFIC ANTIDOTES Idarucizumab: RE-VERSE AD Andexanet alfa PER977 Crowther M et al. Arterioscler Thromb Vasc Biol. 2015; 35:1736-1745

REVERSE-AD A Study of the RE-VERSal Effects of Idarucizumab on Active Dabigatran Patients on dabigatran Active major bleeding Needing emergency surgery Given idarucizumab 5 gm IV Data obtained on bleeding, clotting assays and dabigatran levels Pollack, Jr CV et al. N Engl J Med 2015;373:511-20

Suggested strategy for management of TSOAC-associated bleeding. Siegal D M et al. Blood 2014;123:1152-1158 2014 by American Society of Hematology

CONCLUSIONS 1. These drugs seem to have comparable efficacy when compared with warfarin, with fewer intracranial hemorrhages 2. Risk of major bleeding is reduced with apixaban, but equivalent with rivaroxaban and dabigatran 3. Dabigatran and rivaroxaban seem to increase the rate of GI bleeding 4. We cannot measure levels to adjust for drug interactions 5. The recommendations for reversal of these drugs are based on studies in animals and healthy volunteers, or nonrandomized trials 6. Long term side effects may still be unknown

SUMMARY Novel anticoagulants are appealing, but should be used with caution As TSOACs become more widespread, automated testing will be needed in routine hospital laboratories Bleeding from novel anticoagulants is primarily supportive unless life threatening, in which case PCC or apcc could be used; there are specific antidotes in development