Pointe Scientific, Inc. Instrument Application



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Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Albumin Catalog # : A7502 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Albumin (Pointe) +- Process Protocol Standard Mode Reagent blank Filters: 630-700 Duplicate samples Standard: user defined Normal: 3.5 to 5.3 linear: 0.5 to 8.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. with stats. RPT +- Blank [Type] +- Standard [Type] +-2 ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator -2.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Standard 2.0ul, Speed: 2, Dispense Low +- Level 1 [Type] +- 2 ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 2.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 1 2.0ul, Speed: 2, Dispense Low +- Level 2 [Type]

+- 2 ul sample protocol [Sample Protocol] Aspirate sample Rack Level 2 2.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 2 2.0ul, Speed: 2, Dispense Low +- Standard [Type] +- Unknown [Type] +- 2 ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 2.0ul, Speed: 2, Dispense Low +- 200uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Albumin 200 ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate All 200ul, Speed: 2, Dispense High, Mix +- ncubate 1:00 Rev: 1/03

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Triglycerides Catalog #: T7532 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt mmediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Triglycerides (Pointe) +- Method Standard Mode Reagent Blank Differential Blank Filters: 505 630 Standard: user defined Normal: 36 to 165 Linear: < = 1000 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +- Reagent [Sample Protocol] Aspirate Reagent Rack SQ Triglycerides Reagent 200ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 200 ul, Speed: 1, Dispense High, Mix +- ncubate 2:00 +- Blank [Type] +- Standard [Type]

+-sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Standard 2.5ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Level 1 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 2.5ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Level 2 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 2.5ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 2.5ul, Speed: 0, Dispense High, Mix +- ncubate 10:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Total ron Catalog #: 7505 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Total ron (Pointe) +- Method Standard Mode Reagent Blank Differential Blank Filters: 545 700 Standard: user defined Normal: 60 to 150 Linear: 0 to 500 +- Report Protocol Default Standard. With stats.rpt +- Blank [Type] +- Standard [Type] +-sample [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 40ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard 40ul, Speed: 2, Dispense High +- Level 1 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 40ul, Speed: 2, Air Gap 50ul

Dispense Reaction Plate Level 1 40ul, Speed: 2, Dispense High +- Level 2 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 2 40ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Level 2 40ul, Speed: 2, Dispense High +- Unknown [Type] +-sample [Sample Protocol] Aspirate Sample Rack Unknown 40ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Unknown 40ul, Speed: 2, Dispense High +- 200ul R1 [Sample Protocol] Aspirate Sample Rack SQ ron Buffer 200ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 1, Dispense High, Mix +- ncubate 1:00 +- Blank [Type] +-4ul R2 [Sample Protocol] Aspirate Sample Rack ron Color 4.0ul, Speed: 1, Air Gap 2ul Dispense Reaction Plate Blank 4.0ul, Speed: 0, Dispense High, Mix +- Standard [Type] +-4ul R2 [Sample Protocol] Aspirate Sample Rack ron Color 4.0ul, Speed: 1, Air Gap 2ul Dispense Reaction Plate Standard 4.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-4ul R2 [Sample Protocol] Aspirate Sample Rack ron Color 4.0ul, Speed: 1, Air Gap 2ul Dispense Reaction Plate Level 1 4.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-4ul R2 [Sample Protocol] Aspirate Sample Rack ron Color 4.0ul, Speed: 1, Air Gap 2ul Dispense Reaction Plate Level 2 4.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-4ul R2 [Sample Protocol] Aspirate Sample Rack ron Color 4.0ul, Speed: 1, Air Gap 2ul

Dispense Reaction Plate Unknown 4.0ul, Speed: 0, Dispense High, Mix +- ncubate 10:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Total Protein Catalog #: T7528 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Total Protein (Pointe) +- Process Protocol Standard Mode Reagent Blank Filters: 545 700 Standard: user defined Normal: 6.2 to 8.5 Linear: < = 15.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +- Blank [Type] +- Standard [Type] +-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 4.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Standard 4.0ul, Speed: 2, Dispense Low +- Level 1 [Type] +-4ul sample [Sample Protocol] Aspirate Sample Rack Level 1 4.0ul, Speed: 2, Air Gap 2ul

Dispense Reaction Plate Level 1 4.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-4ul sample [Sample Protocol] Aspirate Sample Rack Level 2 4.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 2 4.0ul, Speed: 2, Dispense Low +- Unknown [Type] +-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 4.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 4.0ul, Speed: 2, Dispense Low +- 200 ul Rgnt [Sample Protocol] Aspirate Reagent Rack SQ Protein Rgnt 200ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix +- ncubate 5:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Total Bilirubin Catalog #: B7576 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Total Bilirubin (1-Part) +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 545 630 Standard: user defined Normal: 0.2 to 1.2 Linear: < = 20.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +- 200ul Rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Total Bilirubin Working Rgnt 200ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High +- Blank [Type] +- Standard [Type]

+-10ul sample protocol [Sample Protocol] Aspirate Sample Rack Bilirubin Calibrator 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Standard 10.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-10ul sample [Sample Protocol] Aspirate Sample Rack Level 1 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 10.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-10ul sample [Sample Protocol] Aspirate Sample Rack Level 2 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 10.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-10ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 10.0ul, Speed: 0, Dispense High, Mix +- ncubate 5:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Phosphorus Catalog #: P7516 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Phosphorus +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 340 630 Standard: user defined Normal: 2.5 to 4.8 Linear: < = 12.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +- 200ul Rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Phosphorus 200ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 1, Dispense High, Mix +- ncubate 2:00 +- Blank [Type] +- Standard [Type]

+-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Standard 4.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-4ul sample [Sample Protocol] Aspirate Sample Rack Level 1 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 1 4.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-4ul sample [Sample Protocol] Aspirate Sample Rack Level 2 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 2 4.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Unknown 4.0ul, Speed: 0, Dispense High, Mix +- ncubate 5:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Magnesium Catalog #: M7527 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Magnesium (Pointe) +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 545-700 Standard: user defined Normal: 1.3 to 2.5 Linear: < = 4.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +-200ul rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Magnesium 200ul, Speed: 1, Air Gap 50ul Dispense Reagent Plate ALL 200ul, Speed: 2, Dispense High, Mix +- ncubate 1:00 +- Blank [Type] +- Standard [Type]

+-2ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Standard 2.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-2ul sample [Sample Protocol] Aspirate Sample Rack Level 1 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 1 2.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-2 ul sample [Sample Protocol] Aspirate Sample Rack Level 2 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 2 2.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-2ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Unknown 2.0ul, Speed: 0, Dispense High, Mix +- ncubate 5:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: auto LDL Catalog #: L7574 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Liquid Auto-LDL +- Method Standard Mode Reagent Blank Differential Blank Filters: 545 700 Standard: user defined Normal: < = 130 Linear: 0 to 700 +- Report Protocol Default Standard. With stats.rpt +- Blank [Type] +- Standard [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack LDL Cholesterol Calib. 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Standard 3.0ul, Speed: 2, Dispense Low +- Lipid Control 1 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Lipid Ctl 1 3.0ul, Speed: 2, Air Gap 2ul

Dispense Reaction Plate Lipid Ctl 1 3.0ul, Speed: 2, Dispense Low +- Lipid Control 2 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Lipid Ctl 2 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Lipid Ctl 2 3.0ul, Speed: 2, Dispense Low +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 3.0ul, Speed: 2, Dispense Low +- 180ul R [Sample Protocol] Aspirate Reagent Rack SQ LDL Cholesterol R1 180ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 180ul, Speed: 1, Dispense High, Mix +- ncubate 5:00 +- 60ul R2 [Sample Protocol] Aspirate Reagent Rack SQ LDL R2 60ul, Speed: 1, Air Gap 40ul Dispense Standard Plate ALL 60ul, Speed: 0, Dispense High, Mix +- ncubate 5:00/Read Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: auto HDL Catalog # :H7545 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Liquid Auto-HDL +- Method Standard Mode Reagent Blank Differential Blank Filters: 600 700 Standard: user defined Normal: < = 35 Linear: 2 to 200 +- Report Protocol Default Standard. With stats.rpt +- Blank [Type] +- Standard [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack HDL Cholesterol Calib. 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Standard 3.0ul, Speed: 2, Dispense Low +- Lipid Control 1 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Lipid Ctl 1 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate - Lipid Ctl 1 3.0ul, Speed: 2, Dispense Low

+- Lipid Control 2 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Lipid Ctl 2 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Lipid Ctl 2 3.0ul, Speed: 2, Dispense Low +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 3.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 3.0ul, Speed: 2, Dispense Low +- 180ul R [Sample Protocol] Aspirate Reagent Rack SQ HDL Cholesterol R1 180ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 180ul, Speed: 1, Dispense High, Mix +- ncubate 5:00 +- 60ul R2 [Sample Protocol] Aspirate Reagent Rack SQ HDL Cholesterol R2 60ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 60ul, Speed: 0, Dispense High, Mix +- ncubate 5:00/Read Rev: 2-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: LDH Catalog # :L7572 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash LDH (Pointe) +- Method Rate by Factor Mode Filters: 340 405 Lag: 60 sec, Read: 180 sec, nterval 60 sec Factor: 3376 (Adjusted for path length: 6010) Total Volume: 210.0ul Units: U/L Normal: 89 to 221 Linear: < = 1000 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-sample 10ul [Sample Protocol] Aspirate Sample Rack Level 1 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 10.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-sample 10ul [Sample Protocol] Aspirate sample Rack Level 2 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 10.0ul, Speed: 2, Dispense Low +- Unknown [Type]

+-sample 10ul [Sample Protocol] Aspirate Sample Rack Unknown 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 10.0ul, Speed: 2, Dispense Low +- 200ul Rgnt [Sample Protocol] Aspirate Reagent Rack SQ LDH Reagent 200ul, Speed: 2, Warm 20s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Glucose Ox Catalog # :G7521 Serum Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Glucose Oxidase (Pointe) +- Process Protocol Standard Mode Reagent blank Filters: 505 630 Standard: user defined Normal: 70 to 115 linear: <= 500 Using Level Controls Using Level Controls +- Report Protocol Default Standard. with stats. RPT +- Blank [Type] +- Standard [Type] +-2.5ul sample [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator - 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Standard 2.5ul, Speed: 2, Dispense Low +- Level 1 [Type] +- 2.5ul sample [Sample Protocol] Aspirate Sample Rack Level 1 2.5ul, Speed: 2, Air Gap 2ul

Dispense Reaction Plate Level 1 2.5ul, Speed: 2, Dispense Low +- Level 2 [Type] +- 2.5ul sample [Sample Protocol] Aspirate Sample Rack Level 2 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 2 2.5ul, Speed: 2, Dispense Low +- Unknown [Type] +- 2.5ul sample [Sample Protocol] Aspirate Sample Rack Unknown 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 2.5ul, Speed: 2, Dispense Low +- 240 ul reagt [Sample Protocol] Aspirate reagent Rack SQ Glucose Oxidase Reagent 240ul, speed:2, Air Gap 50ul Dispense Reaction Plate ALL - 240ul, speed: 2, Dispense High, Mix +- ncubate 10:00 Rev: 2-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Glucose Hex Catalog # :G7517 Serum Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Glucose Hexokinase (Pointe) +- Method Standard Mode Reagent blank Filters: 340 405 Standard: user defined Normal: 70 to 110 linear: <= 500 Using Level Controls Using Level Controls +- Report Protocol Default Standard. with stats. RPT +- Blank [Type] +- Standard [Type] +-sample [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator - 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Standard 2.5ul, Speed: 2, Dispense Low +- Level 1 [Type] +- sample [Sample Protocol] Aspirate Sample Rack Level 1 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 2.5ul, Speed: 2, Dispense Low

+- Level 2 [Type] +- sample [Sample Protocol] Aspirate Sample Rack Level 2 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 2.5ul, Speed: 2, Dispense Low +- Unknown [Type] +- sample [Sample Protocol] Aspirate Sample Rack Unknown 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 2.5ul, Speed: 2, Dispense Low +- 240 ul reagt [Sample Protocol] Aspirate reagent Rack SQ Glucose Hexokinase Reagent 240ul, speed:2, Air Gap 50ul Dispense Reaction Plate ALL - 240ul, speed: 2, Dispense High, Mix +- ncubate 5:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Gamma GT Catalog # :G7571 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 40ul Reagent / Sample low: Look For another Reagent Sample error: Halt mmediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Gamma GT (Pointe) +- Process Protocol Rate by Factor Mode Filters: 405 630 Lag: 90 sec, Read: 120 sec., nterval 60 sec. Factor: 2211 (Adjusted for path length: 3936) Total Volume: 210.0ul Units: U/L Normal: 8 to 54 Linear: < = 1000 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Level 1 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 10.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-sample [Sample Protocol] Aspirate Sample Rack Level 2 10.0ul, Speed: 2, Air Gap 1ul +- Unknown [Type] Dispense Reaction Plate Level 2 10.0ul, Speed: 2, Dispense Low +-sample protocol [Sample Protocol]

Aspirate Sample Rack Unknown 10.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 10.0ul, Speed: 2, Dispense Low +- 200ul Rgnt protocol[sample Protocol] Aspirate Reagent Rack SQ GGT Rgnt 200ul, Speed: 1, Warm 15s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: EA TSH Catalog # :BC1001 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use stored curve by default Warn if curve is 7 days old EA TSH +- Method Pointe To Point Mode Filters: 450-630 Standard 1 = 0.0 Standard 2 = 0.5 Standard 3 = 2.0 Standard 4 = 5.0 Standard 5 = 10.0 Standard 6 = 25.0 Normal: 0.4 to 7.0 Linear: 0.2 to 26.0 +- Report Protocol Default Multistandard.RPT +- Standard 1 [Type] +-100ul sample protocol [Sample Protocol] Aspirate Sample Rack Standard1-100ul, Speed: 2, Air Gap 50ul +- Standard 2 [Type] Dispense Reaction Plate Standard1 100ul, Speed: 2, Dispense High, Mix +-100ul sample protocol [Sample Protocol]

Aspirate Sample Rack Standard2 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard2 100ul, Speed: 2, Dispense High, Mix +- Standard 3 [Type] +- 100ul sample protocol [Sample Protocol] Aspirate Sample Rack Standard3 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard3 100ul, Speed: 2, Dispense High, Mix +- Standard 4 [Type] +- 100ul sample protocol [Sample Protocol] Aspirate sample Rack Standard4 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard4 100ul, Speed: 2, Dispense High, Mix +- Standard 5 [Type] +- 100ul sample protocol [Sample Protocol] Aspirate sample Rack Standard5 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard5 100ul, Speed: 2, Dispense High, Mix +- Standard 6 [Type] +- 100ul sample protocol [Sample Protocol] Aspirate sample Rack Standard6 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Standard6 100ul, Speed: 2, Dispense High, Mix +- Unknown [Type] +- 100ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate Unknown 100ul, Speed: 2, Dispense High, Mix +- TSH Conjugate sample protocol [Sample Protocol] Aspirate Reagent Rack SQ TSH Conjugate 100 ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate All 100ul, Speed: 2, Dispense High, Mix +- ncubate 30:00 +- 4x Wash [Wash Protocol] Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Double Aspirate +- TSH TMB sample protocol [Sample Protocol] Aspirate Reagent Rack SQ TSH TMB 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 100ul, Speed: 2, Dispense High, Mix +- ncubate 10:00

+- TSH Stop sample protocol [Sample Protocol] Aspirate Reagent Rack SQ TSH Stop 50ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 50ul, Speed: 2, Dispense Low, Mix +- ncubate 1:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: EA T3 Catalog # :BC1005 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use stored curve by default Warn if curve is 7 days old EA T3 +- Method Pointe To Point Mode Filters: 450-630 Standard 1 = 0.0 Standard 2 = 0.5 Standard 3 = 1.0 Standard 4 = 2.5 Standard 5 = 5.0 Standard 6 = 10.0 +- Report Protocol Default Multistandard.RPT +- Standard 1 [Type] +-50ul sample protocol [Sample Protocol] Aspirate Sample Rack Standard1-50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard1 50ul, Speed: 2, Dispense High, Mix +- Standard 2 [Type] +-50ul sample protocol [Sample Protocol] Aspirate Sample Rack Standard2 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard2 50ul, Speed: 2, Dispense High, Mix +- Standard 3 [Type]

+- 50ul sample protocol [Sample Protocol] Aspirate Sample Rack Standard3 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard3 50ul, Speed: 2, Dispense High, Mix +- Standard 4 [Type] +- 50ul sample protocol [Sample Protocol] Aspirate sample Rack Standard4 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard4 50ul, Speed: 2, Dispense High, Mix +- Standard 5 [Type] +- 50ul sample protocol [Sample Protocol] Aspirate sample Rack Standard5 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard5 50ul, Speed: 2, Dispense High, Mix +- Standard 6 [Type] +- 50ul sample protocol [Sample Protocol] Aspirate sample Rack Standard6 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Standard6 50ul, Speed: 2, Dispense High, Mix +- Unknown [Type] +- 50ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 50ul, Speed: 2, Air Gap 20ul Dispense Reaction Plate Unknown 50ul, Speed: 2, Dispense High, Mix +- T3 Antibody [Sample Protocol] Aspirate Reagent Rack SQ T3 Antibody 50 ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate All 50ul, Speed: 2, Dispense High, Mix +- ncubate 30:00 +- 4x Wash [Wash Protocol] Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Aspirate/Dispense: 300ul Wash Solution Double Aspirate +- TMB [Sample Protocol] Aspirate Reagent Rack SQ TMB T3 100ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 100ul, Speed: 2, Dispense High, Mix +- ncubate 10:00 +- Stop T3 [Sample Protocol] Aspirate Reagent Rack SQ Stop T3 50ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 50ul, Speed: 2, Dispense Low, Mix

+- ncubate 1:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Creatinine Catalog # :C7539 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 40ul Reagent / Sample low: Look For another Reagent Sample error: Look For another Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Creatinine (Pointe) +- Method Rate by Standard Mode Filters: 505 630 Fixed Time Mode Lag: 50 sec, Read: 150 sec Standard: user defined Normal: 0.4 to 1.4 Linear: < = 25.0 +- Report Protocol Rate Standard. With SD.rpt +- Standard [Type] +-20ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 20ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Standard 20.0ul, Speed: 2, Dispense Low, Mix +- Level 1 [Type] +-20ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 1 20.0ul, Speed: 2, Dispense Low, Mix +- Level 2 [Type]

+-20ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 2 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 2 20.0ul, Speed: 2, Dispense Low, Mix +- Unknown [Type] +-20ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Unknown 20.0ul, Speed: 2, Dispense Low, Mix +- 200ul Reagent [Sample Protocol] Aspirate Reagent Rack SQ Creatinine Rgnt 200ul, Speed: 1, Warm 20s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 1, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: CK-MB Catalog # :C7562 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash CK-MB (Pointe) +- Method Rate by Factor Mode Filters: 340-405 Lag: 300 sec, Read: 180 sec, nterval 60 sec Factor: 13504 (Adjusted for path length: 24625) Total Volume: 205.0ul Units: U/L Normal: 0 to 22 Linear: < = 500 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-Sample 5ul [Sample Protocol] Aspirate Sample Rack Level 1 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 5.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-Sample 5ul [Sample Protocol] Aspirate sample Rack Level 2 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 5.0ul, Speed: 2, Dispense Low +- Unknown [Type]

+-Sample 5ul [Sample Protocol] Aspirate Sample Rack Unknown 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 5.0ul, Speed: 2, Dispense Low +- 200ul Rgnt [Sample Protocol] Aspirate Reagent Rack SQ CK-MB 200ul, Speed: 2, Warm 20s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: CK Catalog # :C7522 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash CK (Pointe) +- Method Rate by Factor Mode Filters: 340-405 Lag: 120 sec, Read: 120 sec, nterval 60 sec Factor: 6592 (Adjusted for path length: 12021) Total Volume: 205.0ul Units: U/L Normal: 0 to 160 Linear: < = 1500 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-sample 5ul [Sample Protocol] Aspirate Sample Rack Level 1 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 5.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-sample 5ul [Sample Protocol] Aspirate sample Rack Level 2 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 5.0ul, Speed: 2, Dispense Low +- Unknown [Type]

+-sample 5ul [Sample Protocol] Aspirate Sample Rack Unknown 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 5.0ul, Speed: 2, Dispense Low +- 200ul Rgnt [Sample Protocol] Aspirate Reagent Rack SQ CK 200ul, Speed: 2, Warm 20s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Cholesterol, Liquid Catalog # :C7510 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Cholesterol Liquid (Pointe) +- Method Standard Mode Reagent Blank Filters: 505-630 Standard: user defined Normal: < = 200 Linear: < = 500 Using Level Controls Using Level Controls +- Report Protocol Default Standard with stats.rpt +- Blank [Type] +- Standard [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate - Standard 2.5ul, Speed: 2, Dispense Low +- Level 1 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 2.5ul, Speed: 2, Air Gap 2ul

Dispense Reaction Plate Level 1 2.5ul, Speed: 2, Dispense Low +- Level 2 [Type] +-sample protocol [Sample Protocol] Aspirate sample Rack Level 2 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 2 2.5ul, Speed: 2, Dispense Low +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.5ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Unknown 2.5ul, Speed: 2, Dispense Low +- 240uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Cholesterol Reagent 240ul, Speed: 2, Air Gap 50ul Dispense Reaction Plate ALL 240ul, Speed: 2, Dispense High, Mix +- ncubate 10:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Calcium CPC Catalog # :C7503/ C7508 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Calcium CPC (Pointe) +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 545-700 Standard: user defined Normal: 8.5 to 10.4 Linear: < = 15.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard with stats.rpt +- 200uL rgnt protocol [Sample Protocol] Wash Probe Aspirate Reagent Rack SQ Calcium CPC Reagent 200ul, Speed: 1, Air Gap 50ul +- Blank [Type] +- Standard [Type] Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix +-4ul sample protocol [Sample Protocol]

Aspirate Sample Rack Chemistry Multi-Calibrator 4.0ul, Speed: 1, Air Gap lul Dispense Reaction Plate - Standard 4.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 1 4.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-4ul sample protocol [Sample Protocol] Aspirate sample Rack Level 2 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 2 4.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-4ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 4.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Unknown 4.0ul, Speed: 0, Dispense High, Mix +- ncubate 1:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Calcium AZ Catalog # :C7529 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Calcium AZ (Pointe) +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 630 700 Standard: user defined Normal: 8.5 to 10.4 Linear: < = 15.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard with stats.rpt +- 200uL rgnt protocol [Sample Protocol] Wash Probe Aspirate Reagent Rack SQ Calcium AZ Reagent 200ul, Speed: 1, Air Gap 50ul +- Blank [Type] +- Standard [Type] Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix +-2ul sample protocol [Sample Protocol]

Aspirate Sample Rack Chemistry Multi-Calibrator. 2.0ul, Speed: 1, Air Gap lul Dispense Reaction Plate - Standard 2.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-2ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 1 2.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-2ul sample protocol [Sample Protocol] Aspirate sample Rack Level 2 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 2 2.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-2ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Unknown 2.0ul, Speed: 0, Dispense High, Mix +- ncubate 1:00 Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: BUN Catalog # :B7552 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default BUN (Pointe) +- Method Rate by Standard Mode Filters: 340 630 Fixed Time Mode Lag: 75 sec, Read: 150 sec Standard: user defined Normal: 7 to 18 Linear: < = 80 +- Report Protocol Ratestandard with SD.rpt +- Standard [Type] +- Sample Protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 2.5ul, Speed: 2, Air Gap lul Dispense Reaction Plate - Standard 2.5ul, Speed: 2, Dispense Low +- Level 1 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 2.5ul, Speed: 2, Dispense Low +- Level 2 [Type]

+-sample protocol [Sample Protocol] Aspirate sample Rack Level 2 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 2.5ul, Speed: 2, Dispense Low +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 2.5ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Unknown 2.5ul, Speed: 2, Dispense Low +- 200uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ BUN Rgnt 200 ul, Speed: 1, Warm 20s, Air Gap 50ul Dispense Reaction Plate All 200ul, Speed: 1, Dispense High, Mix Rev: 4-01

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: AST - SGOT Catalog # : A7561 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 40ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash AST/SGOT (Pointe) +- Method Rate by Factor Mode Filters: 340-405 Lag: 90 sec, Read: 180 sec, nterval 60 sec Factor: -1768 (Adjusted for path length: -3004) Total Volume: 220.0ul Units: U/L Normal: 5-34 Linear: < = 400 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-20 ul sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 1 20.0ul, Speed: 2, Dispense Low, Mix +- Level 2 [Type] +-20 ul sample protocol [Sample Protocol] Aspirate sample Rack Level 2 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 2 20.0ul, Speed: 2, Dispense Low, Mix +- Unknown [Type] +-20 ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 20.0ul, Speed: 4, Air Gap 1ul

Dispense Reaction Plate Unknown 20.0ul, Speed: 2, Dispense Low, Mix +- 200uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ AST Rgnt 200 ul, Speed: 1, Warm 10s, Air Gap 50ul Dispense Reaction Plate All 200ul, Speed: 2, Dispense High, Mix Rev: 1/03

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Amylase Catalog # : A7564 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 25ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Amylase (Pointe) +- Process Protocol Rate by Factor Mode Filters: 405-630 Lag: 60 sec, Read: 120 sec, nterval 60 sec Factor: 3178 (Adjusted for path length: 5795) Total Volume: 205.0ul Units: U/L Normal: 25 to 125 Linear: < = 2000 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 1 5.0ul, Speed: 2, Dispense Low, Mix +- Level 2 [Type] +-sample protocol [Sample Protocol] Aspirate sample Rack Level 2 5.0ul, Speed: 2, Air Gap 1ul Dispense Reaction Plate Level 2 5.0ul, Speed: 2, Dispense Low, Mix +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 5.0ul, Speed: 2, Air Gap 1ul

Dispense Reaction Plate Unknown 5.0ul, Speed: 2, Dispense Low, Mix +- 200uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Amylase 200 ul, Speed: 1, Warm 15s, Air Gap 50ul Dispense Reaction Plate All 200ul, Speed: 2, Dispense High, Mix Rev: 1/03

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: ALT - SGPT Catalog # : A7526 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 40ul Reagent / Sample low: Look For another Reagent Sample error: Halt mmediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash ALT/SGPT (Pointe) +- Method Rate by Factor Mode Filters: 340 405 Lag: 90 sec, Read: 180 sec., nterval 60 sec. Factor: -1768 (Adjusted for path length: -3004) Total Volume: 220.0ul Units: U/L Normal: < = 38 Linear: < = 400 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-20ul sample [Sample Protocol] Aspirate Sample Rack Level 1 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 1 20.0ul, Speed: 2, Dispense Low, Mix +- Level 2 [Type] +-20ul sample [Sample Protocol] Aspirate Sample Rack Level 2 20.0ul, Speed: 4, Air Gap 1ul Dispense Reaction Plate Level 2 20.0ul, Speed: 2, Dispense Low, Mix +- Unknown [Type] +-20ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 20.0ul, Speed: 4, Air Gap 1ul

Dispense Reaction Plate Unknown 20.0ul, Speed: 2, Dispense Low, Mix +- 200ul Rgnt [Sample Protocol] Aspirate Reagent Rack SQ ALT Working Rgnt 200ul, Speed: 1, Warm 10s, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix Rev: 1/03

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Alkaline Phosphatase Catalog # : A7516 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 40ul Reagent / Sample low: Look For another Reagent Sample error: Halt immediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Alk Phos (Pointe) +- Process Protocol Rate by Factor Mode Filters: 405-630 Lag: 60 sec, Read: 120 sec, nterval 60 sec Factor: 2187 (Adjusted for path length: 3988) Total Volume: 205.0ul Units: U/L Normal: 35 to 123 Linear: < = 1000 +- Report Protocol Ratefactor with SD.rpt +- Level 1 [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Level 1 5.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 1 5.0ul, Speed: 2, Dispense Low +- Level 2 [Type] +-sample protocol [Sample Protocol] Aspirate sample Rack Level 2 5.0ul, Speed: 2, Air Gap 2ul Dispense Reaction Plate Level 2 5.0ul, Speed: 2, Dispense Low +- Unknown [Type] +-sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 5.0ul, Speed: 2, Air Gap 2ul

Dispense Reaction Plate Unknown 5.0ul, Speed: 2, Dispense Low +- 200uL rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Alk Phos 200 ul, Speed: 1, Warm 15s, Air Gap 50ul Dispense Reaction Plate All 200ul, Speed: 2, Dispense High, Mix Rev: 1/03

Pointe Scientific, nc. nstrument Application Analyzer: Chemwell Test: Uric Acid Catalog #: U7581 Options: Assignment Order: 8-way in groups of 4 Optimized order execution Temperature Control to 37C Dispense push Volume 15ul Reagent / Sample low: Look For another Reagent Sample error: Halt mmediately Bottle 1 (Wash) low: Halt at end of wash Bottle 2 (Rinse) low: Halt at end of wash Use new curve by default Use new blank by default Uric Acid (Pointe) +- Process Protocol Standard Mode Reagent Blank Differential Blank Filters: 505 630 Standard: user defined Normal: 2.5 to 7.7 Linear: < = 25.0 Using Level Controls Using Level Controls +- Report Protocol Default Standard. With stats.rpt +- 200ul Rgnt protocol [Sample Protocol] Aspirate Reagent Rack SQ Uric Acid Rgnt 200ul, Speed: 1, Air Gap 50ul Dispense Reaction Plate ALL 200ul, Speed: 2, Dispense High, Mix +- ncubate 1:00 +- Blank [Type] +- Standard [Type]

+-5ul sample protocol [Sample Protocol] Aspirate Sample Rack Chemistry Multi-Calibrator 5.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Standard 5.0ul, Speed: 0, Dispense High, Mix +- Level 1 [Type] +-5ul sample [Sample Protocol] Aspirate Sample Rack Level 1 5.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 1 5.0ul, Speed: 0, Dispense High, Mix +- Level 2 [Type] +-5ul sample [Sample Protocol] Aspirate Sample Rack Level 2 5.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Level 2 5.0ul, Speed: 0, Dispense High, Mix +- Unknown [Type] +-5ul sample protocol [Sample Protocol] Aspirate Sample Rack Unknown 5.0ul, Speed: 1, Air Gap 1ul Dispense Reaction Plate Unknown 5.0ul, Speed: 0, Dispense High, Mix +- ncubate 10:00 Rev: 4-01