If several different trials are mentioned in one publication, the data of each should be extracted in a separate data extraction form.

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General Remarks This template of a data extraction form is intended to help you to start developing your own data extraction form, it certainly has to be adapted to your specific question. Delete unnecessary information and include all information important for your field. It is advisable to use one data-extraction form for one study, so that one data-extraction form may contain the information gained from several publications on the same trial. If several different trials are mentioned in one publication, the data of each should be extracted in a separate data extraction form. Fill in every field as it must be obvious from the form if a certain information is missing or uninterpretable (versus forgotten to extract) Extract all information that you will need for further analysis (e.g. subgroup analysis) and which allow you to classify or group several studies with common features (e.g. study quality, protocol of intervention) Specify which information is unclear or name conflicting details in order to avoid duplication of effort Extraction of statistics: extract all information on variables on location and variability, standard error, confidence interval and p-values. Extract exact figures of p-values (instead of [not] significant ) and add niveau of confidence (95 or 99%) Abbreviations: REF ID NR IN EX DB References Identification Not reported Included excluded Database CHMG 2007 1

STUDY ELIGIBILITY FORM FACTORS ASSESSMENT COMMENTS TYPE OF STUDY 1. Is the study described as randomized? Yes Unclear No NB. Please answer No if the study is a crossover or quasi-randomized trial. Exclude PARTICIPANTS 2. Were participants diagnosed as patients with disease of interest? Yes Unclear No Exclude 3. Were participants of the prespecified age? Yes Unclear No Subgroups available? NB: Please answer Yes, If mix age participants i.e. both >18 years and < 18 years are included and state it as comments. No: If only < 18 years. INTERVENTIONS Exclude 4. Were comparison groups treated with prespecified intervention in one group and control intervention in other group? NB: study can have 3 arms e.g. CT arm, CT+RT (CMT) arm or RT arm, if so please cross Yes and state it as comments. OUTCOMES Yes Unclear No Exclude 5. Did the study report prespecified outcomes? Yes Unclear No Exclude FINAL DECISION 1 X No = EXCLUDE 1 X Unclear = UNCLEAR CHMG 2007 2

ORGANISATIONAL ASPECTS EX IN REF ID Reviewer, Date Checked by Author, Year Journal/Source Study ID NR / Country of origin Publication type Fulltext / Abstract / Book chapter / internal progress report other (please specify) Other relevant publications in DE-form Decision pending / Check references / Use for discussion / Fate EX without listing / EX with listing / Other (please specify) Notes / Short description REASONS FOR EXCLUSION OF STUDY FROM REVIEW (PLEASE SPECIFY according to protocol Methods No RCT / Inadequate concealment of allocation / Other Patients Outcomes Different disease / stage / pre schedule / age Subgroups available? No clinically relevant outcomes assessed No data for relevant subgroup extractable Other Duplicate publication / Other NONE Included CURRENT STATUS: (NAME OF REVIEWER + DATE) Question to clinician Question to author Status verified with study investigators or sponsors: Yes / No Enter name of the source (e.g. PI, sponsor, etc.) Contact address: CHMG 2007 3

STUDY INTERVENTION BASICS Disease(s)/stage(s) studied Category of investigated First line therapy / Consolidation therapy Salvage therapy Other: Inclusion criteria Exclusion criteria Specials: Experimental Intervention If more than two, please specify/add further rows Intervention Control Type of control Active / Placebo / Active + placebo / No therapy Additional Balanced between arms? Y / N Compliance Evaluated? Y / N Planed in case of failure/as long-term? Outcomes assessed Treatment arms comparable? Subgroup evaluated Confounders Infection related mortality Infection incidence Neutropenia incidence Neutropenia duration Treatment-related mortality Response Overall survival Event-free survival Progression-free survival Adverse events Quality of life Other (please specify) Significant differences between arms: (extractable data for these subgroups) (were confounders mentioned? A priori / a posteriori? Which? Multivariate analysis?) CHMG 2007 4

TRIAL CHARACTERISTICS Sample size Randomised / recruited Number of excluded patients Recruitment method consecutive inclusion Setting in-patient / out-patient / unclear / NR Location of trial Dates of Recruitment Trial Design Length of follow-up Phase Parallel / cross-over / Factorial Single center / Multicenter trial: international / national / # centers: Equivalence/Non-inferiority Multi-arm study: Yes / No If yes:, how many? From till Median (range): Mean: Funding Industry / Public / mixed (industry supported: drug / data management / travel / salary / other unclear / NR Conflict of interest statement Yes / No / NR Number of groups Flow diagram? Method of randomisation Method of concealment of allocation Blinding Central Methods NR / Minimization / Inadequate (e.g. date of birth, visit) Stratified by Adequate (please specify): Done +unclear / Not done / inadequate (e.g. differently coloured envelopes) Single / double / triple / not possible (1: patient only; 2: + physician; 3: + outcome-assessor) Primary study aims NR (if not reported, leave out primary and fill in secondary study aims) Secondary study NR aims CHMG 2007 5

Statistically significant for primary end-point: Yes / No / Enter p-value: Statistically significant for secondary end-point: Yes / No / Enter p-value: Systematic Review on Intervention X in patients Y If outcome was NOT statistically significant is it because due to Outcomes Evidence of absence of effect (true negative study) (i.e. clearly defined based on primary outcomes in the trial), or No evidence for absence of effect (i.e. inconclusive, or lowpowered study) (i.e. not clearly defined or not based on primary outcomes in the trial) Unclear No / Yes (expected effect: ) Power calculation? Statistical methods Expected difference on primary outcome: Yes / No Enter Value: Alpha (α) pre-specified: Yes / No Enter value: Beta error (β) pre-specified: Yes / No Enter value: Calculated sample size: Yes / No Enter value: Sample Size achieved? Yes / No ITT as treated per protocol unclear Analysis (+ definition) Definition: available and acceptable not available Different endpoints with different analysis? Please specifiy Stopping rules Drop outs stated No / Yes BASELINE CHARACTERISTICS OF PATIENTS Experimental Arm Control Arm Others Notes;p-values Overall comment Number of patients No significant differences Age -- -- Reports how to transform units, all ± values = means mean/± ± ± median/± ± ± Ethnicity No. % Gender No. % NR Male: Female: NR Male: Female: CHMG 2007 6

Diagnosis Definition of Diagnosis Extent of disease Organ involvement Additional diagnoses in group Stage Staging system Status of patients at Rdx e.g. untreated Previous Concurrent conditions Considered as high risk patients Considered as low risk patients Laboratory parameter (UNITS) -- -- Cytogenetics Performance status Source BASELINE CHARACTERIZATION OF PATIENTS (continued) Often, the patients characteristics are summarised in a table to very different extent in different studies. Instead of extracting every single figure, it might be useful only to extract the type and the number of baseline characteristics that have been evaluated and if there were differences between groups. If you want to use the figures for the formation of subgroups, however, it is advisable to extract them and to let them be checked for accuracy! Information Evaluated Statistically significant differences between groups Important prognostic factor A Info 1 Notes CHMG 2007 7

Info 2 Primary intervention (Medication, dosage, administration) Treatment Details according to STUDY PROTOCOL (as planned) Experimental Arm Control Arm Others Notes; The form can be adapted to list expected medication and or schedules in order to reduce the amount of necessary writing. Timing of Duration of (days, cycles) Important information e.g. bone marrow or peripheral blood stem cells Treatment specials Supportive Patient flow according to PUBLICATION (as it really happened) Experimental Arm Control Arm Others Notes; p-values No. of patients screened No. of patients recruited No. of patients allocated No. of patients evaluated No. of patients receiving planned Reasons for not receiving No. of dropouts Reasons for drop-outs CHMG 2007 8

No. of protocolviolations Type and percentage of salvage / unplanned OUTCOMES The following tables have to be copied as many times as there are outcomes assessed. OUTCOME Outcome Primary Secondary not defined Definition of outcome (Check definitions carefully and compare to definitions of outcome you have specified in your protocol for the meta-analysis) Timing of assessment Statistics Length of follow-up No. of patients evaluated for this outcome All randomised Unclear Less [%] Reasons for drop-out Reasons for exclusion Source of information NR NR Dichotomous data Outcome Time Intervention group Control Arm Notes Observed events Sample size Observed events Sample size Source text, p figure No., table No. Expert statistical attention needed? Y / N Continuous data Outcome Time Intervention group Experimental Arm Notes, p Sample size Mean/ mean change (incl. Range) Standard Deviation Sample size Mean /mean change (incl. Range) Standard Deviation CHMG 2007 9

Notes Source text, p figure No., table No. Survival probabilities Outcome Time Patients at risk Intervention group (incl. CI) Patients at risk Control Arm Rate [%] of patients alive / Rate [%] of patients alive / SE / Sample size/ %CI SE / Sample size/ %CI OS PFS Notes Source text, p figure No., table No. Notes P value Median / Mean duration of survival Patients at risk Intervention group Duration (months/years) Patients at risk OS PFS Notes Source text, p figure No., table No. Control Arm Duration (months/years) Notes P value CHMG 2007 10

Randomization ratio Patients randomised Patients analysed Observed Deaths Logrank expected events HR (CI 95% or standard error or variance from Cox) Logrank variance Logrank O-E test statistik (& test used, 1 or 2 sided?) Advantage to control or research? Kaplan-Meier curves or Actuarial curves? Numbers at risk reported? Follow up details Calculation of Hazard ratio for e.g. Death (table derived from i and ii ) Arm 1 (CSF) Arm 2 (Control) Arm 3 Arm 4 Estimates for Death: HR Lower 95% CI Upper 95% CI ln(hr) se(ln(hr)) Variance O-E Definition of death: CHMG 2007 11

METHODOLOGICAL QUALITY - OVERVIEW REF ID Reviewer, Date Checked by Author, Year Journal/Source Study ID NR / Publication type Fulltext / Abstract / Other (please specify) yes unclear no Comments Randomization Treatment allocation Similarity of groups Implementation of blinding Transparent patient flow? Completeness of trial ITT (less than 15% loss) Loss to follow up symmetric in both arms? Different drop-out rates for different endpoints? Treatment preference (see below) Type of primary endpoint Hard Soft Summarized validity: Low risk of bias Moderate risk of bias High risk of bias Remarks: Randomization: Yes: random numbers, etc. - No: patient number, day of week, etc. Unclear: method not stated Allocation concealment: Yes: central, No: alternate, etc. Unclear: not stated Similarity of groups: Were the participant characteristics at baseline similar in both groups regarding the most important prognostic factors? Blinding: Was the allocation masked at the outcome assessments/to data managers? Transparency: Were withdrawals, drop-outs and patients lost to follow-up stated for each group? (Yes if there were no drop-outs, withdrawals etc.) Completeness: If transparent, drop-out rate per study < 15%?), if asymmetric, please specify in comments ITT: Did the analysis include an ITT analysis and were there less than 10% of patients excluded in each group? Comment if appropriate definition of ITT CHMG 2007 12

Treament preference: 1 standard highly preferred - 2 standard preferred to innovation 3 about equal, innovation a disappointment - 4 about equal, innovation a success 5 innovation preferred to standard 6 experimental highly preferred Type of endpoint: hard e.g. mortality, survival i Parmar MK, Torri V, Stewart L. Extracting summary statistics to perform meta-analyses of the published literature for survival endpoints. Stat Med 1998; 17(24):2815-2834. ii Tierney JF, Stewart LA, Ghersi D, Burdett S, Sydes MR. Practical methods for incorporating summary time-to-event data into meta-analysis. Trials 2007; 8:16. CHMG 2007 13