Dosing Principles and Dosage Form Considerations. Objectives. Disclaimer. In converting a patient who has



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Dosing Principles and Dosage Form Considerations for BHRT Texas Osteopathic Medical Association Dallas, TX June 16, 2011 Jim Paoletti, Pharmacist, FAARFM ZRT Laboratory The following potential conflict of interest relationships are germane to my presentation: Equipment: None Speaker Bureau: ZRT Laboratory Stock Shareholder: none Grant/Research Support: none Consultant: ZRT Laboratory Status of FDA devices used for the material being presented: N/A Status of off label use of devices, drugs, or other materials that constitute the subject of this presentation: N/A Disclaimer Employee of ZRT Laboratory ZRT Laboratory is a testing Laboratory for hormones, cardiometabolic risks factors, Vitamin D Objectives Discuss the popular dosage forms used for BHRT and the applications, advantages and disadvantages of each Review practical considerations to take into account with topical hormone preparations p Discuss considerations in changing therapy from one route of delivery to another In converting a patient who has been on CEE/MPA for years to bioidentical therapy: a) Immediately stop MPA and start progesterone b) Immediately stop CEE and change to bi est c) Increase bi est dose until hot flashes are well controlled d) Stop CEE immediately but taper off MPA What is the best dosage form for progesterone adminstration? a) Oral Sustained Release Capsule b) Oral oil filled capsule c) Sublingual toche c) Sublingual toche d) Topical cream e) Topical gel f) None of the above Copyright 2011 Jim Paoletti All Rights Reserved 1

How often should a sublingual troche be administered a) Once daily b) Twice a day c) Three times a day d) Every 2 3 hours Oral estrogen therapy results in: a) Decrease in clotting risks b) Decrease in to E1 ratio c) Decrease in CBG d) Decrease in TBG e) Decrease in SHBG Physiologic Bio identical Hormone Restoration Therapy The goal of BHRT is to restore hormone levels to the normal physiological level of a younger individual to provide the protective benefits of the hormones to the entire system. Dosage Adjustment Adjusting dosage for hormone restoration therapy individually requires assessment of therapeutic outcome and levels Monitor symptoms Retest to determine if physiological levels obtained If symptoms not resolved at normal physiologic levels, other causes should be considered: Example: hot flashes with optimal estrogen levels: consider high cortisol, low thyroid, excessive progesterone, poor diet and exercise Dosing Guidelines (Female) Peri Menopause Oral administration of SR capsules Progesterone 25 to 400 mg daily (usual 100 to 200 mg) Dose once or twice a day Givecyclically days14 through 25 Bi estrogen 50:50 If estrogen levels confirmed low If progesterone alone doesn t control symptoms 0.1mg to 0.5 mg daily Dose once or twice a day Give cyclically days 1 through 25 Continue progesterone as above Continuous vs. Cyclic Dosing Hormones do not have to be given in cyclic fashion to provide benefits of restoration therapy Continuous dosing take a break 2 5 days per month Copyright 2011 Jim Paoletti All Rights Reserved 2

Conversion to BHRT Considerations in conversion from synthetics to bio identical hormones No true equivalency Length of time on synthetics Estrogen receptors can lose sensitivity due to exposure to high amounts and/or long exposure of estrogen replacement (any type of estrogen) Liver detox Conversion to BHRT Taper off high estrogen dose Elevated threshold in brain for estrogen Withdrawal symptoms can be severe and highly individual Start progesterone immediately Discontinue any progestin Address other hormone imbalances that may be adding to symptoms Insulin resistance, adrenals, thyroid and androgens Conversion Considerations Progesterone Progesterone affects estrogen effects Regulates estrogen receptors Has effects on SHBG, thyroid and cortisol actions all effect estrogen deficiency dfii symptoms Start progesterone prior to switching from synthetic estrogen to bio identical estrogen Discontinue any synthetic progestins Conversion Considerations Estrogen Taper off of synthetic estrogen Higher than physiological levels reset the threshold for estrogen need within the brain Quick reduction in dosage will cause withdrawal symptoms (severe hot flashes) Various protocols Example: decrease conventional estrogen therapy by one half dose every 3 days, then 2 out of three days, then daily, etc Example: Premarin give ½ tab QD x 2 wks then ½ tab QOD x 2 wks Use ½ dose every 3 rd day for 9 12 days, then ½ dose 2 of 3 days for 9 12 days, then ½ dose daily, then ½ dose 2 out of 3 days for 9 12 days, then ½ dose every other day. When low dose is reached, switch to Bi est 50:50 Stronger ratio of to E3 than Bi est 80:20 Conversion Considerations Estrogen Patient compliance Let patient determine how quickly then can decrease dose Patients on synthetics for a long duration may take longer to convert Dosage considerations Start bio identical estrogen dosage at mid range Difficult withdrawal usually requires higher dose of bioidentical Consider changing estrogen ratio Biest 40:60, 30:70, 20:80 (E3:) Copyright 2011 Jim Paoletti All Rights Reserved 3

Converting Administration Routes Dosage comparisons difficult due to multiple influences on relative bio availabilities Approximate ratio for topical:s/l: oral For progesterone, estrogen, DHEA: topical 1: sublingual 2: oral 4 5 For testosterone: top 1: s/l 2: oral 5 6 Startingguideline only Converting Administration Routes Switching to topical dosing may require higher initial dosing Estrogen: 5 10 days Progesterone and testosterone: 3 to 6 weeks DHEA:? Lab results may be confusing Vaginal Administration Good absorption systemically Documented effectiveness for Progesterone in luteal phase defect Commercial products for Estrogens Used for systemic and/or local effects Dosage forms Suppositories Base MBK or Base A Creams or gels Vaginal troches Progesteron ne (ng / ml) Plasma Levels of Progesterone after Nahoul Vaginal and Diagrams plasma Oral Administration of Progesterone DOC 4 3 2 1 0 Vaginal Oral 0 2 4 6 24 26 28 30 48 Hours Nahoul et al. Profiles of plasma estrogens, progesterone, and their metabolites after oral or vaginal administration of estradiol and progesterone. Maturitas 16: 185-202, 1993 Oral Dosage Forms General Considerations Micronized vs. non micronized Use source of consistent quality chemical Size & percentage of micronization is critical Higher production of metabolites Oral Dosage Forms Immediate release capsules 95% destroyed in upper GI tract before entering portal system High peak increases risk of side effects Would have to dose more frequently No good reason to use Copyright 2011 Jim Paoletti All Rights Reserved 4

Oral Dosage Forms Sustained release capsules Provides good level response Less failures and side effects from peak and valley effect of other dosage forms Easier to titrate dosage to an individual Most women can dose once or twice a day IJCP article validates use of Methocel E4M in compounded capsules to provide sustained release Oral dosage forms Oil filled capsule Absorption through lymphatic system bypasses GI destruction and first past effect to some degree Not sustained release May have to dose 2 to 4 times a day More time consuming to compound Prometrium Peanut oil base 10% bio available Sublingual / Buccal Administration Advantages Avoid upper GI tract destruction Avoid first pass effect Sublingual / Buccal Administration Disadvantages May not get true sublingual absorption regularly May have to dose more often Taste may not be acceptable May not be convenient to patient Sublingual / Buccal Administration Drops Vehicle choices Aqueous suspensions or emulsions Alcohol / Glycerin mixtures Oils Concentration Flavor vs. convenience Should use small volume, 0.2 0.25 max. Concentrated taste harder to mask Pellet Implants Requires surgical procedure and device May cause infection or irritation Surgery to remove High supraphysiologic doses could occur Initial surge then fall off in effect Too much may down regulate receptors Implanted every 3 6 months usually Copyright 2011 Jim Paoletti All Rights Reserved 5

Pellet Implants May be optimal dosage form for elderly, non compliant patient (Alzheimer's) Expensive equipment required to compound Limited number of compounders make pellets Some physicians report great clinical results May take time to see full effect of hormones May require priming with progesterone May require patience with androgens Cream bases allow for more depot effect than transdermal or gel bases Requires less total daily hormone dosing Transference of hormone is always a concern Cannot use serum or urine testing to see tissue (effective) level of hormone of BHRT Steroids are low molecular weight, highly lipophyllic molecules, therefore, good candidates for absorption across the skin Use of a transdermal base not required for absorption Permeability (Scheuplein Units cm/hr) Aldosterone 3.0 x 10 6 Hydrocortisone 3.0 x 10 6 Estriol 4.0 x 10 5 Estradiol 3.0 x 10 4 Increasing Testosterone 4.0 x 10 4 Permeability Pregnenolone 1.5 x 10 3 Progesterone 1.5 x 10 3 Estrone 3.6 x 10 3 Johnson ME, et al. J Pharm Sci 84: 1144 1146, 1995 Permeation of Steroids through Human Skin Application considerations Skin cement and adipose tissue and may act as natural reservoir for hormones Drained by the lymphatic system Depot effect Target of application to skin should be lipophyllic tissue, not the bloodstream Use of a transdermal penetrating base may decrease depot effect of dosage applied Application considerations Site of application Common: inside of arm, back of upper arm, upper thigh Avoid transference: back of knee, buttocks, covered site Rotation of application site Specific site based on clinical use Copyright 2011 Jim Paoletti All Rights Reserved 6

Cream vs. Gel Emulsion cream bases without penetration enhancers added allow for more deposition to skin cement and fat storage sites Compared to an equivalent dose in a gel base: Somewhat slower absorption, longer activity Tk Takes longer to achieve symptom relief Can accumulate to supraphysiologic levels if dosed too high Will not see change in serum level Start dose higher than anticipated maintenance dose for a short time, then reduce Cream vs. Gel Gels contain the penetration enhancer alcohol Compared to an equivalent dose in a cream base: Somewhat faster absorption Less deposition in skin cement and adipose tissue Faster reliefof symptoms Less likely to accumulate with time May see change in serum level May have to dose higher and/or more often Faster absorption, less depot, metabolized sooner Repeated use has drying effect on skin Bases commonly used Oil in water emulsions Emollient cream base Vanishing cream base Combinations Gels Carbopol, HEC, HPC Usually contain alcohol Special HRT bases Formulated for good absorption into skin Absence of irritating and sensitizing agents Maintenance dosage may be less than starting dosage Method of application can vary degree of depot effect Concentration Volume (area covered) Penetration properties of base Solubility of drug Packaging considerations Accurate measurement of dosage Ability to adjust dosage Prevent contamination Consultation considerations Compliance most important Do not adjust dosage without supervision Accurate measurement Accurate measurement Rub in well for at least one minute Caution on possible transfer of hormone to others Copyright 2011 Jim Paoletti All Rights Reserved 7

Injections High supraphysiologic doses given High level initially followed by drop off until next dose Convenient? Given much less often Administered in doctor s office Compounded products Fixed Oil or Aqueous suspensions Different salts/esters have different durations Rectal Administration Good absorption Avoid 2/3 rd of first pass effect Rectal suspension administered i dwith syringe and rectal tip Inconvenient for patient Patient variability high Oral vs. Topical administration of hormones is more efficient than oral Skin delivery is a more natural route of delivery Oral Estrogen Therapy High conversion of estradiol to estrone Giving enough oral estradiol to obtain a level in the low end of a normal pre menopause level of estradiol will result in a corresponding elevated estrone level Usually are not measuring estrone, and rarely measure estrone sulfate Cannot mimic the natural ratio of estrogens with oral therapy Estradiol (), Estrone (E1) and Oral Estrogen Supplementation Serum (pg/ml) Serum E1 (pg/ml) /E1 Premenopause 60 60 1 Postmenopause 20 60 0.33 Oral Estradiol 60 300 0.2 Oral vs. Topical Estrogens Oral E may reduce IGF 1 and increase GH levels One study demonstrated transdermal does too Oral E may reduce postprandial lipid oxidation Reduction in lean mass and increase in fat mass Transdermal E lowers antithrombin III levels which are associated with severe and recurrent venous thrombosis Copyright 2011 Jim Paoletti All Rights Reserved 8

Oral vs. Topical Estrogens Oral administration of estrogen increases HDL but also increases triglycerides Topical estrogen shows no changes in HDL or triglycerides gy Both routes of E reduce LDL, VLDL, and Lp(A), Topical estrogens improve appearance and function of skin Oral vs. Topical Progesterone Oral Progesterone produces more metabolites than topical Larger dosage required because of loss of 90 95% with GI destruction and first pass effect Allopregnenolone works on GABA receptor Sedative and hypnotic properties Different metabolites produced some that inhibit and some that increase cancer tumor growth Topical progesterone may also increase appearance of skin Topical Hormones Topical testosterone may increase hair density and growth (where you don t want it) Not much is known about the metabolism of hormones that may occur in the skin Never been shown to be a significant effect Dosage Form Considerations Summary Oral route should utilize SR and consistent source of micronized hormone Will increase metabolites compared to other routes S/L or Buccal will require more frequent dosing Cannot use saliva testing of free hormones Injections cause peaks and troughs of hormones and metabolites Topical route results in less metabolites Cannot use urine or serum testing to adjust Most important factor in choice is patient compliance Relative Potency of Estrogens Estradiol () is 12x more potent than estrone (E1) and 80x more potent than estriol (E3).as measured by action on the uterine tissue. Vaginal/Urogenital tissue responds better to estriol Estradiol estimated by some to be 1000x more potent in regards to proliferative effect Copyright 2011 Jim Paoletti All Rights Reserved 9

Considerations in Estrogen Replacement Therapy Considerations in Estrogen Replacement Therapy The three major 100 estrogens produced 80 by our bodies: 60 % 40 Estrone (E1) 20 Estradiol () 0 Estriol (E3) E1 E3 Estrone Estradiol Estriol 100 The three major 80 estrogens produced 60 % by our bodies: 40 Estrone (E1) 20 0 Estradiol () Estriol (E3) E1 E3 Estrone Estradiol Estriol Wright, Jonathan V. Altern Med Rev 1999;4(4):266-270 Xu X,Duncan AM,Merz-Demlow BE, Phipps W, Kurzer MS. Menstrual Cycle Effects on Urinary Estrogen Metabolites Clin Endocrin Metab Nov 1999;84(11) J Bio identical Estrogens Original Formulations (80% Estriol) Human Estrogen Estradiol 10% Estrone 10% Estriol 80% Tri Est (original) 1.0 mg Estradiol 0.1 mg (10%) Estrone 0.1 mg (10%) Estriol 0.8 mg (80%) Bi est (original) 1.0mg Estradiol 0.2 mg (20%) Estriol 0.8 mg (80%) Bio identical Estrogens 50% Estriol Formulas Human Estrogen Estradiol 6% Tri Est 0.2 mg Estradiol 0.05 mg (25%) Estrone 60% Estrone 0.05 mg (25%) Estriol 34% Estriol 0.1 mg (50%) Bi est 0.2mg Estradiol 0.1 mg (50%) Estriol 0.1 mg (50%) Biest Formulas There is NO standard ratio Prescribe/label in a manner that clearly indicates your intention or preparation Ex: Biest: 0.1 mg/e3 0.1 mg or Ex: Biest 0.2 mg ( 0.1/E3 0.1) Prescribe/label individual amounts Avoid percentages! The Influence of Estriol Copyright 2011 Jim Paoletti All Rights Reserved 10

The Influence of Estriol The Influence of Estriol E3: 1.0 mg Bi est 80:20 Content E3 0.4mg Bi est 50:50 E3 E3 E3 In converting a patient who has been on CEE/MPA for years to bioidentical therapy: a) Immediately stop MPA and start progesterone b) Immediately stop CEE and change to bi est c) Increase bi est dose until hot flashes are well controlled d) Stop CEE immediately but taper off MPA What is the best dosage form for progesterone adminstration? a) Oral Sustained Release Capsule b) Oral oil filled capsule c) Sublingual toche d) Topical cream e) Topical gel f) None of the above How often should a sublingual troche be administered a) Once daily b) Twice a day c) Three times a day d) Every 2 3 hours Copyright 2011 Jim Paoletti All Rights Reserved 11

Oral estrogen therapy results in: a) Decrease in clotting risks b) Decrease in to E1 ratio c) Decrease in CBG d) Decrease in TBG e) Decrease in SHBG Thanks For Listening!!! Jim Paoletti Jim Paoletti ZRT Laboratory 503 597 1865 jepaoletti@zrtlab.com Copyright 2011 Jim Paoletti All Rights Reserved 12