RESUME HELENE HEGARET, Ph.D.

RESUME HELENE HEGARET, Ph.D. Centro de Investigación y Desarrollo en Recursos y Ambientes Costeros, i~mar Universidad de Los Lagos Camino a Chiquihue Km 6, Casilla 557 Puerto Montt CHILE Tel: (+56) (9) 949 223 58 Helene.Hegaret@gmail.com Nationality: French Date of Birth: August 9 th 1979 French: mother tongue English: fluent (reading, writing, speaking) Spanish: fluent (reading, writing, speaking) Portuguese: basic level German: basic level Driving licence (B and D1 licences) Boating certificate (coastal and offshore licences) Diving certification (Rescue PADI) Scientific diver in training (Rescue certification) CPR, First Aid EDUCATIONAL BACKGROUND 1997 Baccalauréat (French National High School Diploma equivalent of English A-levels) Specialization in Biology, Math and Chemistry (Tréguier High School) 2003 National Diploma of Engineer in Agricultural Engineering in Fisheries Sciences from Ecole Nationale Supérieure Agronomique de Rennes in France (equivalent to a Master Degree) 2007 M.S. in Oceanography (specialty in Marine Biology) at the University of Connecticut in the Department of Marine Sciences with Dr. S.E. Shumway 2007 Ph.D. in Oceanography (specialty in Marine Biology) at the University of Connecticut in the Department of Marine Sciences with Dr. S.E. Shumway 2007 Ph.D. in Halieutique (=Aquaculture and Fishery Sciences) at Ecole Nationale Supérieure Agronomique de Rennes (ENSAR) in France with Dr. J.F. Samain (double Ph.D. agreement) PROFESSIONAL EXPERIENCE 2001 Undergraduate research at ENSAR with Dr. G. Fontenelle. Project : Study on shrimp fishing and aquaculture in Madagascar 2002 Undergraduate research Participation to a collaborative project on Domestication of moluscan shellfish between France and the USA, working with Dr. J.F. Samain (IFREMER) et G.H. Wikfors (NOAA).Project: Internship on the interaction between immunology and nutrition of the Eastern oyster, Crassostrea virginica: - five months at National Oceanic and Atmospheric Administration (NOAA), National Marine Fisheries Service Laboratory, Milford, USA, with Dr. G.H. Wikfors - five months in IFREMER and in IUEM (Institut Universitaire et Européen de la Mer) in Brest, France with Dr. J.F. Samain and Dr. P. Soudant. 2002 Undergraduate research at Concepto Azul, a private firm developing sustainable biotechnology, working with Dr. E. Miahle on diagnostics of diseases of the shrimp Penaeus vannamei in Ecuadorian aquaculture, and at the National Park of Galapagos to develop methods in molecular biology. Project: Develop molecular method to detect the presence of White Spot virus in shrimp Penaeus vannamei 1

2003 Graduate research at NOAA, National Marine Fisheries Service Laboratory, Milford, with Dr. G.H. Wikfors in USA, Project: Effect of harmful algal blooms on immune response of oysters 2003 Research Assistant on a research cruise in the Arctic Ocean, working with Dr. P Renault (University of Connecticut). Project: Study of benthic communities and their implications in the carbon processes in Artic region. 2004-2007 Graduate research Ph.D. research at the University of Connecticut, USA and the Ecole Nationale Supérieure Agronomique de Rennes, France with Dr. S.E. Shumway and J.F. Samain. Project done in cooperation with the NOAA - Milford National Marine Fisheries Service Laboratory in the USA, with Dr. G.H. Wikfors, with the IUEM (European Institute for Marine Studies) in Brest, in France, working with Dr. P. Soudant (CNRS French National Research Center) and with the Marine Biological Laboratory in the USA, with Dr. R. Smolowitz. Project : Impacts of harmful algal blooms on the cellular and physiological response of bivalve molluscs. 2004-2007 Graduate research - Participation to a research project on Ecology of Harmful Algal Blooms (ECOHAB), in collaboration with Dr. S.E. Shumway, G. Wikfors and J. Burkholder Project : Potential introductions of new harmful phytoplankton species via transfert and tranport of bivalve molluscs. 2005-2007 Graduate research - Participation to a research project on National Research Council of Aquaculture (NRAC) in collaboration with Dr. R. Smolowitz, S. Shumway, G. Wikfors, D. Leawitt and B. Walton Project: Effect of temperature and population on progression of the parasite Quahog Parasite Unkown (QPX) in northern quahog Mercenaria mercenaria. 2007-2008 Teaching and research assistant at the University of Brest, at the Technological Institute (IUT) and at the European Institute for Marine Studies, at the Laboratoire des sciences de l'environnement marin (IUEM, LEMAR, UMR 6539)) with Dr. P. Soudant. Project Allelopathic interactions between several harmful phytoplankton species. 2008 Post-Doctoral research at the Universidad de Los Lagos (Centro I-MAR), in Puerto Montt, Chile with Dr. A. Buschmann and Dr. D.Varela. Project: Effect of the harmful dinoflagellate Alexandrium catenella and its toxin on larval development of king crabs Lithodes santolla. AWARDS AND HONORS «Ulysse» fellowship from France, 2002 ( 900) Gordon Gunter Award (Best Student Poster), National Shellfisheries Association: 2003, 2005 and 2006 Student Travel Awards to attend National Shellfisheries Assoc. Annual Meetings: 2003, 2005, 2006 Melbourne R. Carriker Student Research Grant, National Shellfisheries Association Grant Program (Honorable Mention), 2005 Lerner-Gray Marine Research Fellowship, American Museum of Natural History, 2005 ($1000) Alan Ansell Award (Best Student Poster), 15th International Pectinid Workshop, Australia, 2005 Castagna Award for Applied Research, National Shellfisheries Association, 2006 ($1000) Sigma Xi Grants-in-Aid of Research, 2006 ($900) Elected to membership in Sigma Xi, 2006 EPA Science Forum Poster Award, session Changing Environments, USA 2006 Feng Student Fund Research Award from University of Connecticut, 2006 ($1260) Travel award from the National Oceanic and Atmospheric Administration Center for Sponsored Coastal 2

Ocean Research to attend the 12th International Conference on Harmful Algae in Copenhagen, Denmark, 2006 ($725) PreDoctoral Research Award, Graduate School of the Department of Marine Sciences, University of Connecticut, 2006 ($1240) Melbourne R. Carriker Research Grant, National Shellfisheries Association, 2007 ($1000) World Aquaculture Society, CRSP Poster Award, Aquaculture Conference, USA, 2007 Elected to membership in Phi Beta Kappa honor society, 2007 Elected to membership in Phi Kappa Phi honor society, 2007 Best Student Presentation Award, 16th International Pectinid Workshop, Canada, 2007 Doctoral dissertation fellowship award of the University of Connecticut, 2007 ($2000) Travel award from the National Oceanic and Atmospheric Administration Center for Sponsored Coastal Ocean Research to attend the US Conference on Harmful Algae in Woods Hole, MA, 2007 ($725) Lavoisier Fellowship (EGIDE) from the French «Ministère des Affaires Etrangères et Européennes» for post-doctoral studies at the Universidad de Los Lagos in Chile ( 11500). NOAA Fisheries Service Team Member of the Year Award, 2007 TEACHING AND ADVISORING EXPERIENCE Teaching Experience: 2002: Laboratory instructor in Master 1 in the University of Brest on use of flow cytometry (4h) 2003: Undergraduate-level courses in cellular and animal biology at the University of Connecticut (2h) 2003 and 2004: Laboratory instructor during the Milford Aquaculture Laboratory Algal Workshop (10h) 2004: Laboratory instructor for one semester for undergraduate level classes Biology 107 (60h) 2004: Instructor for three courses at the undergraduate level for the class Biology 107 (5h) 2007: Laboratory instructor for Ground Science (36h) 2008: Laboratory instructor for Biochemistry (30h) 2008: Laboratory instructor for Conversational English (80h) Intern advisor: 2005: 3 months N. Saliou: Potential transfer of harmful algal blooms via relocation of bivalve molluscs 2005: 3 months M. Gonçalves: Effect of harmful algae on the hemocyte response of bivalve molluscs. 2006: 6 months L. Chemin: Mitigation of risk of transfer of harmful algal blooms via relocation of bivalve molluscs PUBLICATIONS (12 published, 1 in revision, 3 submitted) 1. Hégaret, H., Wikfors, G.H. and Soudant, P., 2003. Flow-cytometric analysis of haemocytes from eastern oysters, Crassostrea virginica, subjected to a sudden elevation in temperature: I. Haemocyte types and morphology. Journal of Experimental Marine Biology and Ecology 293, 237-248. 2. Hégaret, H., Wikfors, G.H., and Soudant, P., 2003. Flow-cytometric analysis of hemocytes from eastern oysters, Crassostrea virginica, subjected to a sudden, sudden temperature elevation: II. Hemocyte functions: aggregation, viability, phagocytosis and respiratory burst. Journal of Experimental Marine Biology and Ecology 293, 249-265. 3. Hégaret, H., Wikfors, G.H., Soudant, P., Delaporte, M., Alix, J.H., Smith, B.C., Dixon, M.S., Quére, C., Le Coz, J.R., Paillard, C., Moal, J. and Samain, J.-F., 2004. Immunological competence of eastern oysters, Crassostrea virginica, fed different microalgal diets and challenged with a temperature elevation. Aquaculture, 234, 541-560. 3

4. Hégaret, H., and Wikfors, G.H., 2005. Time-dependent changes in hemocytes of eastern oysters, Crassostrea virginica, and northern bay scallops, Argopecten irradians irradians, exposed to a cultured strain of Prorocentrum minimum. Harmful Algae, 4 (2), 187-199. 5. Hégaret, H., and Wikfors, G.H., 2005. Effects of natural and field-simulated blooms of the dinoflagellate Prorocentrum minimum upon hemocytes of eastern oysters, Crassostrea virginica, from two different populations. Harmful Algae, 4 (2), 201-209. 6. Buggé, D.M., Hégaret, H., Wikfors, G.H., and Allam, B., 2007. Oxidative burst in hard clam (Mercenaria mercenaria) haemocytes. Fish and Shellfish Immunology, 23: 188-196. 7. Hégaret, H., Wikfors, G.H., Soudant, P., Lambert, C., Shumway, S. E., Bérard, J.B., and Lassus, P., 2007. Toxic dinoflagellates (Alexandrium fundyense and A. catenella) have minimal apparent effect on oyster hemocytes. Marine Biology 152(2), 441-447. 8. Hégaret, H., Wikfors, G.H. and Shumway, S. E., 2007. Feeding behavior of five species of bivalve mollusc exposed to three species of harmful algae. Journal of Shellfish Research 24(2) 549-559 9. Hégaret, H., da Silva P. M., Wikfors, G.H., Lambert, C., De Bettignies, T., Shumway, S. E. and Soudant, P., 2007. Hemocyte responses of Manila clams, Ruditapes philippinarum, with varying parasite, Perkinsus olseni, severity to toxic-algal exposures. Aquatic toxicology 84: 269-279. 10. da Silva P. M., Hégaret, H., Lambert, C., Wikfors, G.H., Le Goïc, N., Shumway, S. E. and Soudant, P., 2008. Immunological responses of the Manila clam (Ruditapes philippinarum) with varying parasite (Perkinsus olseni) burden, during a long term exposure to the harmful alga, Karenia selliformis and possible interactions. Toxicon 51(4), 563-573. 11. Hégaret, H., Shumway, S. E., Wikfors, G.H., Pate, S., and Burkholder, J.A.M. (2008). Potential transport of harmful algae through relocation of bivalve molluscs. Marine Ecology Progress Series 361: 169-179. Galimany, E., Sunila, I., Hégaret, H., Ramón, M., Wikfors, G.H. (2008). Pathology and immune response of the blue mussel (Mytilus edulis L.) after an exposure to the harmful dinoflagellate Prorocentrum minimum. Harmful Algae 7: 630-638. 12. Galimany, E., Sunila, I., Hégaret, H., Ramón, M., Wikfors, G.H. (2008). Experimental exposure of the blue mussel (Mytilus edulis, L.) to the toxic dinoflagellate Alexandrium fundyense: histopathology, immune responses, and recovery. Harmful Algae 7: 702-711. 13. Hégaret, H., da Silva P. M., Sunila, I., Shumway, S. E., Dixon, M.S., Alix, J.H., Wikfors, G.H. and Soudant, P., (in revision). Perkinsosis in the Manila clam Ruditapes philippinarum affects responses to the harmful-alga, Prorocentrum minimum. Journal of Experimental Marine Biology and Ecology 14. Hégaret, H., da Silva P. M. (submitted). In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs. Fish and Shellfish Immunology 15. Hégaret, H., Smolowitz, R.M.,, Sunila, I., Shumway, S. E., Alix, J.H., Dixon, M.S. and Wikfors, G.H., (submitted). Combined effects of a parasite, QPX, and the harmful-alga, Prorocentrum minimum on northern quahogs, Mercenaria mercenaria. Aquatic toxicology 16. Pate, S., Burkholder, J.A.M., Shumway, S. E., Wikfors, G.H., Frank, D., Hsia, M., Hégaret, H. Dorch Q. (submitted). Effects of the toxic dinoflagellate Alexandrium monilatum on survival, grazing and behavioral response of three ecologically important shellfish species. Toxicon 4

CONFERENCE PROCEEDINGS (2) 1. Hégaret, H., Shumway, S. E., and Wikfors, G.H. Harmful algae can be transported via relocation of bivalve shellfish. Proceedings of the 12th International Conference on Harmful Algae, Copenhagen, Denmark. (in press) 2. Hégaret, H., Wikfors, G.H., and Shumway, S. E. In vitro interactions between several species of harmful algae and hemocytes of bivalve molluscs. Proceedings of the 12th International Conference on Harmful Algae, Copenhagen, Denmark. (in press) BOOK CHAPTER (1) 1. Hégaret, H., Shumway, S. E., Wikfors, G.H. (in press). Biotoxin contamination and shellfish safety. In Shumway, S.E. and G.E. Rodrick (eds). Shellfish Safety and Quality, Woodhead Publishing Limited, UK UNPUBLISHED TECHNICAL REPORTS (3) 1. Hégaret, H., 2008. Impact of harmful algal blooms on physiological and cellular responses of bivalve molluscs. Ph.D. Thesis. University of Connecticut. 2. Hégaret, H., 2003. Immune response of the molluscan bivalves, Eastern oysters Crassostrea virginica and Northern Bay scallops Argopecten irradians irradians, to natural and simulated summer dinoflagellate blooms. Mémoire de fin d études pour l obtention du Diplôme d Agronomie Approfondie, ENSAR. 3. Lassus P., Abadie E., Amzil Z., Antoine E., Bardouil M., Baron R., Bornet B., Masselin P., Nézan E., Séchet V., Sibat M., Truquet P., Bérard J.B., Gueguen M., Le Gall C., Ringelstein J., Fourgeaud E., Toledano N., Schmidt A., Defontaine A., Kermarrec L., Hégaret H., Soudant P., Barillé L., and Cognie B. Proliférations toxiques d Alexandrium catenella dans l étang de Thau : modèle de contamination des coquillages. Rapport Final. Chantier «Lagunes Méditerranéennes» Programme National Environnement Côtier Atelier 2.2, 2006. PUBLISHED ABSTRACTS (22) 1. Hégaret, H., Wikfors, G.H., Soudant, P., Delaporte, M. Moal, J. and Samain, J.F. Interactions between nutrition and immune response in the Eastern Oyster, Crassostrea virginica: A well-fed oyster is a healthy oyster, n est ce-pas? J. Shellfish Res.,21: 342-343. 2. Wikfors, G.H., Alix, J.H., Smolowitz, R.M., Wallace, L., and Hégaret, H. 2002. Detrimental effects of a recent Prymnesium isolate from Boothbay Harbor, Maine (USA), upon juvenile bay scallops, Argopecten irradians. J. Shellfish Res. 21: 397. 3. Hégaret, H., Wikfors, G.H., Soudant, P., and Samain, J.F. 2003. Algal food quantity and quality affect immune function in oysters stressed by high temperature. J. Shellfish Res.,22: 334-335. 4. Hégaret, H., and Wikfors, G.H. 2004. Time-dependent changes in hemocytes of eastern oysters, Crassostrea virginica, and northern bay scallops, Argopecten irradians irradians, exposed to a cultured strain of Prorocentrum minimum. J. Shellfish Res. 23: 637. 5. Wikfors, G. H., Smolowitz, R. M., Hégaret, H., and Alix, J. H. 2004. The estuarine dinoflagellate, Prorocentrum minimum HAB or HAB-not? J. Shellfish Res., 23: 645. 6. Hégaret, H., and Wikfors, G.H. 2005. Immune response of two populations of the Eastern oyster Crassostrea virginica, to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum. J. Shellfish Res., 24: 656-657. 5

7. Hégaret, H., and Wikfors, G.H. 2005. Immune response of two populations of the Eastern oyster Crassostrea virginica, to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum. J. Shellfish Res., 24: 656-657. 8. Hégaret, H., and Mazurié J. 2005. Restoration of oyster production in France by introduction of non-native species: history and overview. J. Shellfish Res., 24: 1263. 9. Hégaret, H., Wikfors, G.H., Soudant, P., Lambert, C., Bérard, J.B., and Lassus, P. 2005. Two species of the toxic dinoflagellate Alexandrium have no apparent effect on immune systems of the oysters Crassostrea virginica anf Crassostrea gigas. J. Shellfish Res., 24: 657. 10. Hégaret, H., Shumway, S.E., and Wikfors, G.H. 2005. Potential vector of harmful algae through bivalves. J. Shellfish Res., 24: 657. 11. Gonçalves, M., Wikfors, G.H., Alix, J.H., Hégaret, H. 2006. Functions of granular and agranular hemocytes in eastern oysters following flow-cytometric live-sorting. J. Shellfish Res. 25:662. 12. Hégaret, H., Shumway, S.E., Saliou, N., and Wikfors, G.H. 2006. Transport of harmful algae via relocation of bivalve molluscs. J. Shellfish Res. 25: 664. 13. Li, Y., Hégaret, H., Meseck, S.L., Wikfors, G.H., Dixon, M.S., and Alix, J.H. 2006. Differences in protein excretion by the harmful marine dinoflagellate, Prorocentrum minimum, under different culture conditions, as revealed by SELDI proteomic technology. J. Shellfish Res. 25: 666 14. Smith, B.C., Hégaret, H., Saliou, S., and Wikfors, G.H. 2006. A portable, experimental shellfish feeding system: Design and validation of operation. J. Shellfish Res. 25: 673. 15. Wikfors, G.H., Sunila, I., Alix, J.H., Croxton, A.N., Hégaret, H., and Meseck, S.L. 2006. Immune-status assessments of oysters from the 2004 State of Connecticut, Bureau of Aquaculture survey. J. Shellfish Res. 25: 676-677. 16. Hégaret, H., Shumway, S.E., and Wikfors, G.H. 2006. Bivalve shellfish can be vectors of transport of harmful algae. J. Shellfish Res. 25: 738. 17. Hégaret, H., Wikfors, G.H., Gonçalves, M., and Shumway, S.E. 2006. Effect of in vitro interactions between Mercenaria mercenaria hemocytes and several species of harmful algae. J. Shellfish Res. 25: 738-739. 18. Chemin, L., Wikfors, G.H., Hégaret, H. and Shumway, S.E. Development of best management practices (BMPS) to mitigate the risk of transplanting harmful algae along with bivalve molluscan shellfish. J. Shellfish Res. 26: 654. 19. Hégaret, H., Wikfors, G.H., Gonçalves M., P. M. da Silva, P. Soudant, and Shumway S.E. 2007. In vitro interactions between several species of harmful algae and hemocytes of two species of clams. J. Shellfish Res. 26: 659 20. Hégaret, H., Shumway S.E., Wikfors, G.H. 2007. Effects of harmful algae on physiology and hemocyte parameters of the Northern Bay scallop, Argopecten irradians irradians. J. Shellfish Res. 26: 1316 21. Hégaret, H., Smolowitz, R.M., Wikfors, G.H., DeFaveri, J., Walton, W., Murphy, D., Shumway, S.E. Effect of temperature on hemocyte responses of northern quahogs (=hardclams, Mercenaria mercenaria) from different populations, infected by the protistan organism, Quahog Parasite Unknown. J. Shellfish Res. 27: 1016 22. Wikfors, G.H., Hégaret, H., Gamilany, E., Sunila, I., Soudant, P., Shumway, S.E. Hemocyte responses in bivlve molluscs exposed to harmful or toxic algae; overview and preliminary synthesis. J. Shellfish Res. 27: 1063 6

ORAL PRESENTATIONS AT CONFERENCES (30) February 2002 Interactions between nutrition and immune response in the Eastern Oyster, Crassostrea virginica: A well-fed oyster is a healthy oyster, n est-ce-pas? Milford Aquaculture Seminar, USA. Hégaret*, H., Wikfors, G.H., Soudant, P., Delaporte, M. Moal, J. and Samain, J.F. April 2002 Detrimental effects of a recent Prymnesium isolate from Boothbay Harbor, Maine (USA), upon juvenile bay scallops, Argopecten irradians. National Shellfisheries Association Annual Meeting, Mystic, CT, USA. Wikfors*, G.H., Alix, J.H., Smolowitz, R.M., Wallace, L., and Hégaret, H. February 2004 Immune response of two populations of the Eastern oyster, Crassostrea virginica, subjected to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum Milford Aquaculture Seminar, USA. Hégaret*, H., and Wikfors, G.H. April 2004 May 2004 The estuarine dinoflagellate, Prorocentrum minimum HAB or HAB-not? Milford Aquaculture Seminar, USA. Wikfors*, G. H., Smolowitz, R. M., Hégaret, H., and Alix, J. H.. Time-dependent changes in hemocytes of eastern oysters, Crassostrea virginica, and northern bay scallops, Argopecten irradians irradians, exposed to a cultured strain of Prorocentrum minimum. Northeast Algal Symposium in Groton, Avery Point, CT, USA. Hégaret*, H., and Wikfors, G.H. Immune response of two populations of the Eastern oyster, Crassostrea virginica, exposed to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum. Feng colloquium, University of Connecticut - Avery Point, CT, USA. Hégaret*, H. November 2004 Restoration of oyster production in France by introduction of non-native species: history and overview. Invited Planery Talk. International Conference of Shellfish Restoration, Charleston, SC, USA. Hégaret*, H., and Mazurié, J. February 2005 Restoration of oyster production in France by introduction of non-native species: history and overview. Milford Aquaculture Seminar, USA. Hégaret*, H., and Mazurié, J. April 2005 October 2005 Immune response of two populations of the Eastern oyster Crassostrea virginica, to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum. National Shellfisheries Association meeting, Philadelphia, USA. Hégaret*, H., and Wikfors, G.H. Transport of harmful algae via relocation of bivalve molluscs International Conference of Shellfish Restoration, Brest, France Hégaret*, H., Shumway, S. E., Wikfors, G.H., Pate, S., Burkholder, J.A.M. Harmful algal roadblocks to eastern oyster restoration Prorocentrum minimum and Karlodinium micrum International Conference of Shellfish Restoration, Brest, France Wikfors*, G. H., Ozbay, G., Place, A. R., Li, J.Y., Meseck, S.L., Hégaret, H., and Alix, J.H. 7

February 2006 Transport of harmful algae via relocation of bivalve molluscs Milford Aquaculture Seminar, USA. Hégaret*, H., Shumway, S. E., Saliou N, and Wikfors G.H. March 2006 Relocation of bivalve molluscs can be vectors of transport of harmful algae Benthic Ecology Meeting, Quebec, Canada. Hégaret*, H., Shumway, S. E., Wikfors, G.H., Pate, S., and Burkholder, J.A.M. Effect of in vitro interactions between Mercenaria mercenaria hemocytes and several species of harmful algae National Shellfisheries Association meeting, Monterey, USA. Hégaret*, H., Wikfors, G.H., Gonçalves M., and Shumway S.E. November 2006 Harmful Algal Blooms, Reproduction and Recruitment in Molluscan Shellfish Physiological aspects of reproduction and nutrition in mollusks Workshop, La Paz, Mexico. Shumway* S.E., and Hégaret, H. December 2006 In vitro interactions between several species of harmful algae and hemocytes of two species of clams. Northeast Aquaculture Conference and Exposition, Mystic, USA. Hégaret*, H., Wikfors, G.H., Gonçalves M., P. M. da Silva, P. Soudant, and Shumway S.E. December 2006 Relations algues toxiques (Karenia selliformis) - palourde Ruditapes ssp.: Emphase sur l infection par Perkinsus olseni. ECOBIN-Atelier franco-canadien sur l'évaluation environnementale par biomarqueurs écotoxicologiques, Brest, France, 2006. da Silva*, P. M., Hégaret, H., De Bettignies, T., and Soudant, P. Effects of toxic algae Karenia selliformis on haemocyte parameters of Manila Clam Ruditapes philippinarum and its response to Perkinsus olseni infection. International Workshop on Summer Mortality of Marine Shellfish, Busan, South Korea, 2006. da Silva*, P. M., Hégaret, H., Le Goic, N., Paillard, C., Wikfors, G.H., and Soudant, P. Development of best management practices (BMPS) to mitigate the risk of transplanting harmful algae along with bivalve molluscan shellfish Northeast Aquaculture Conference and Exposition, Mystic, USA. Chemin*, L., Wikfors, G.H., Hégaret, H. and Shumway, S.E. February 2007 Combined effects of Perkinsus olseni and the harmful algae Karenia spp. on immune parameters of Manila clams World Aquaculture and National Shellfisheries Association meeting, San Antonio, USA. Hégaret*, H., da Silva*, P. M., Soudant, P., Lambert, C., and Wikfors, G.H. May 2007 Flow-cytometric applications for bivalve hemocytes: current status and future directions World Aquaculture and National Shellfisheries Association meeting, San Antonio, USA. Wikfors, G.H., Hégaret*, H., Soudant, P., Gonçalves M., Alix, J.and Pitchford, S. Effects of harmful algae on physiology and hemocyte parameters of the northern bay scallop, Argopecten irradians irradians 16th International Pectinid Workshop, Canada. Hégaret*, H., Shumway, S.E., and Wikfors, G.H. Best Student Presentation Award, 16th International Pectinid Workshop, Canada, 2007 8

May 2007 Interactions algues toxiques bivalves. Atelier international sur l évaluation environnementale par mesure de biomarqueurs écotoxicologiques chez des espèces sentinelles bivalves. ECOBim 2007, Rimouski (Canada). Lambert*, C., Haberkorn, H., Hégaret, H., Da Silva, M., Bricelj, M., Ford, S., Barbou, A., Auffret, M., Le Goïc, N., Paillard, C. and Soudant, P October 2007 Assessment of the potential for introduction of harmful algal bloom (HAB) species via shellfish transport. Hégaret*, H., Shumway, S. E., Wikfors, G.H., Pate, S., Burkholder, J.A.M. EPA NE Science Day, Providence, RI, USA November 2007 Interactions entre les dinoflagellés (Karenia selliformis, Prorocentrum minimum) et un parasite de la palourde Perkinsus olseni. Hégaret*, H., da Silva P. M., Smolowitz, R.M., Sunila, I., Wikfors, G.H., Dixon, M., Haberkorn, H., Shumway, S. E., Soudant, P. Mini-workshop Interactions eucaryotes unicellulaires, Brest, France. November 2007 Effect of the harmful algal Prorocentrum minimum on the hemocyte response of quahogs Mercenaria mercenaria with various levels of QPX infection. Hégaret*, H., Wikfors, G.H., Smolowitz, R., Shumway, S. E. Fourth Symposium on Harmful Algae in the U.S., Woods Hole, MA, USA. November 2007 Mitigating the risk of introducing harmful algae during relocation of bivalves. Hégaret*, H., Shumway, S. E., Wikfors, G.H. Estuarine Research Federation Conference, Providence, RI, USA March 2008 April 2008 Effet couple d un parasite Perkinsus olseni et d une microalgue toxique (Prorocentrum minimum ou Karenia selliformis) sur les réponses histologiques et hémocytaires des palourdes Hégaret, H., da Silva, P.M., Sunila, I., Wikfors, G.H., Dixon, M.S., Haberkorn, H., Shumway, S.E. Soudant*, P. Workshop on «impact des organismes pathogènes et des micropolluants sur l état de santé des poissons, mollusques et crustacés des milieux naturels», Nantes, France Effect of temperature on hemocyte responses of northern quahogs (=hardclams, Mercenaria mercenaria) from different populations, infected by the protistan organism, Quahog Parasite Unknown Hégaret*, H., Smolowitz, R.M., Wikfors, G.H., DeFaveri, J., Walton, W., Murphy, D., Shumway, S.E. National Shellfisheries Association meeting, Providence, USA Hemocyte responses in bivalve molluscs exposed to harmful or toxic algae; overview and preliminary synthesis. Wikfors*, G.H., Hégaret, H., Galimany, E., Sunila, I., Soudant, P., Shumway, S.E. National Shellfisheries Association meeting, Providence, USA September 2008 Apoptosis signaling by oxidized polyunsaturated fatty acids: possible roles in trophic interactions between bivalves and food particles. Wikfors*, G.H., Alix, J.H., Arbaouil, R., Sunila, I., Galimany, E., Hégaret, H. Physiomar, Brest, France 9

INVITED ORAL PRESENTATIONS (8) September 2003 Immune response of the molluscan bivalves, Eastern oysters Crassostrea virginica and Northern Bay scallops Argopecten irradians irradians, to natural and simulated summer dinoflagellate blooms. Hégaret*, H. Ecole Nationale Agronomique de Rennes, France. May 2004 January 2006 January 2007 Immune response of two populations of the Eastern oyster, Crassostrea virginica, exposed to natural and simulated blooms of the dinoflagellate, Prorocentrum minimum. Hégaret*, H. Université de Bretagne Occidentale, Brest, France Transport of harmful algae via relocation of bivalve molluscs Hégaret*, H., Shumway, S. E., Wikfors, G.H., Pate, S., and Burkholder, J.A.M. Institut Universitaire Européen de la Mer, Brest, France Review of the effects of harmful algal blooms on reproduction and early development of bivalve molluscs Hégaret*, H., Shumway, S. E. Université de Bretagne Occidentale, Brest, France December 2007 Effets des efflorescences de microalgues toxiques sur les réponses physiologiques et cellulaires des mollusques bivalves. Hégaret*, H. Institut Universitaire Européen de la Mer, Brest, France May 2008 June 2008 Mitigación del riesgo de introducción de algas nocivas asociadas con transporte de bivalvos. Hégaret*, H., Wikfors*, G.H. and Shumway, S.E. Universidad de Los Lagos, Puerto Montt, Chile Impacts of harmful algal blooms on cellular and physiological processes of bivalve molluscs: The example of northern bay scallop, Argopecten irradians irradians. Hégaret*, H., Wikfors*, G.H. and Shumway, S.E. Universidad de Concepción, Chile September 2008 Impacto de las floraciones algales nocivas sobre los procesos celulares y fisiológicos de moluscos bivalvos. Hégaret*, H. Universidad Católica del Norte, Coquimbo, Chile POSTER PRESENTATIONS AT CONFERENCES (17) May 2003 Algal food quantity and quality affect immune function in oysters stressed by high temperature. National Shellfisheries Association meeting, New Orleans, USA. Hégaret*, H., Wikfors, G.H., Soudant P. and Samain, J.F. Gordon Gunter Poster Award, National Shellfisheries Association Annual Meeting, 2003 December 2003 Study of the immune responses of the bivalves Crassostrea virginica and Argopecten irradians irradians exposed to natural and artificial blooms of the dinoflagellate, Prorocentrum minimum. Second Symposium on Harmful Marine Algae in the US, Woods Hole, MA, USA. Hégaret, H., and Wikfors*, G.H. 10

November 2004 Study of the immune responses of the bivalves Crassostrea virginica and Argopecten irradians irradians exposed to natural and artificial blooms of dinoflagellates, Prorocentrum minimum. International Harmful Algal Bloom Conference, Cape Town, South Africa Hégaret, H., Wikfors G. H. and Shumway* S.E. April 2005 April 2005 October 2005 Bivalves as a potential vector of transport of harmful algae. Benthic ecology meeting, USA. Hégaret, H., Shumway*, S.E., and Wikfors, G.H. Two species of the toxic dinoflagellate Alexandrium have no apparent effect on immune systems of the oysters Crassostrea virginica anf Crassostrea gigas. National Shellfisheries Association meeting, Philadelphia, USA. Hégaret*, H., Wikfors, G.H., Soudant, P., Lambert, C., Berard, J.B., and Lassus, P. Potential vector of harmful algae through bivalves National Shellfisheries Association meeting, Philadelphia, USA. Hégaret*, H., Shumway, S.E., and Wikfors, G.H. Gordon Gunter Poster Award, National Shellfisheries Association Annual Meeting, 2005 The effect of harmful algal blooms on the immune response of Argopecten irradians irradians. 15th International Pectinid Workshop, Australia. Hégaret, H., Shumway*, S.E., and Wikfors, G.H. Alan Ansell Poster Award, 15th International Pectinid Workshop, Australia, 2005 Bivalve shellfish can serve as vectors for transport of harmful algae. The Third Symposium on Harmful Algae in the U.S., Monterey, CA, USA Shumway*, S. E., Hégaret, H., Wikfors, G.H., and Burkholder, J.A. M. October 2005 Two species of the toxic dinoflagellate Alexandrium have no apparent effect on immune systems of the oysters Crassostrea virginica anf Crassostrea gigas. The Third Symposium on Harmful Algae in the U.S., Monterey, CA, USA Hégaret*, H., Wikfors, G.H., Soudant, P., Lambert, C., Berard, J.B., Lassus, P., and Shumway, S.E. March 2006 May 2006 Bivalve shellfish can be vectors of transport of harmful algae National Shellfisheries Association meeting, Monterey, CA, USA. Hégaret*, H., Wikfors, G.H., and Shumway S.E. Gordon Gunter Poster Award, National Shellfisheries Association Annual Meeting, 2006 Potential Transport of Harmful Algae through Relocation of Bivalve Molluscs EPA Science Forum, Washington, USA. Hégaret*, H., Wikfors, G.H., Shumway S.E., Pate, S., and Burkholder, J.A. M. EPA Science Forum Poster Award, session Changing Environments, 2006 Bivalve shellfish can be vectors of transport of harmful algae Feng colloquium, University of Connecticut, Avery Point, USA. Hégaret*, H. September 2006 Transport of harmful algae via relocation of bivalve molluscs 12th International Conference on Harmful Algae, Copenhagen, Denmark Shumway, S.E., Hégaret, H., and Wikfors, G.H. In vitro interactions between several species of harmful algae and hemocytes of bivalve molluscs 12th International Conference on Harmful Algae, Copenhagen, Denmark Hégaret*, H., Wikfors, G.H., and Shumway S.E. 11

February 2007 Mitigation of risk of transplanting harmful algae along with transport of bivalve shellfish. World Aquaculture and National Shellfisheries Association meeting, San Antonio, USA. Hégaret*, H., Chemin, L., Wikfors, G.H. and Shumway, S.E. World Aquaculture Society, CRSP Poster Award, Aquaculture Conference, USA, 2007 Mars 2007 Bivalve mollusc relocation: a potential vector of harmful-algal introductions Benthic Ecology Meeting, Atlanta, USA. Hégaret*, H., Shumway S.E., Wikfors, G.H., Pate, S., and Burkholder, J.A. M. November 2007 Mitigating the risk of introduction of harmful algae via relocation of bivalve molluscs. Shumway, S. E., Hégaret*, H., Wikfors, G.H. Fourth Symposium on Harmful Algae in the U.S., Woods Hole, MA, USA MISCELLANEOUS INFORMATION Member of the organizing committee of national & international conferences: Rencontres Halieutiques, conférence à Rennes, France, 2002. International Conference on Shellfish Restoration à Brest, France, 2005. Workshop sur les interactions entre unicellulaires hétérotrophes» à Brest, 2007. Member of several associations National Shellfisheries Association Sigma Xi Phi Kappa Phi Phi Beta Kappa Reviewer of scientific journals : Aquatic Biology Harmful Algae Journal of Environmental Marine Biology and Ecology Journal of Shellfish Research Journal of World Aquaculture Society Collaborations: Dr. Sandra Shumway, University of Connecticut, Groton, CT, USA Dr. Gary Wikfors NMFS, NOAA, Milford, CT, USA Dr. Philippe Soudant, CNRS, Université de Bretagne Occidentale, Brest, France Dr. Roxanna Smolowitz Marine Biological Laboratory, Woods Hole, MA, USA Dr. Sylvain De Guise University of Connecticut, Groton, CT, USA Dr. Patrick Lassus, Ifremer, Nantes, France Dr. Inke Sunila, DEP Connecticut, Milford, CT, USA Dr. Bassem Allam Stony Brook University, Stony Brook, NY, USA Dr. P. Mirella da Silva Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil Dr. Alejandro Buschmann, Universdad de Los Lagos, Puerto Montt, Chile Dr. Daniel Varela, Universdad de Los Lagos, Puerto Montt, Chile Dr. Miriam Seguel, Universdad Austral, Puerto Montt, Chile 12