Overview of the Galveston National Laboratory



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Overview of the Galveston National Laboratory 1

Galveston National Laboratory One of two national laboratories constructed with support from NIH. The GNL is a global leader in the study of highly pathogenic microbes. UTMB s investigators are working to solve some of the most difficult health concerns of our time. They are also producing solutions to problems which will benefit the health of the world community. 2

The mission of the Galveston National Laboratory is to assist the NIAID and the nation by conducting basic and applied research designed to improve the prevention, diagnosis and treatment of naturally emerging and purposefully disseminated infectious diseases. 3

Hedrich Blessing Photography 2009 BSL-2 4

Hedrich Blessing Photography 2009 BSL-3 5

Hedrich Blessing Photography 2009 BSL-4 6

Galveston National Laboratory Department Name Goes Here 7

BSL4 Maximum Containment Department Name Goes Here 8

BSL-4 laboratory PPE ILC Dover suit (10-18 lbs; external HEPA filter; Chlorinated polyethylene) Sperian suit (8-10 lbs; HEPA filter integrated into suit; polyamide/polyvinyl chloride) 9

Hedrich Blessing Photography 2009 HEPA exhaust filters 10

Effluent Decontamination System 11 Hedrich Blessing Photography 2009

Hedrich Blessing Photography 2009 Emergency power for the entire lab 12

Basic Research Product Development NIH R01 and other Funded Research Proof of Concept Concept Validation Pre-clinical/ GLP Studies Human Clinical Trials Animal Rule GNL UTMB Phase I: Safety Phase II: Expanded Safety Phase III: Efficacy FDA Restricted Use Approval 13 FDA Licensure

New drugs to treat Ebola Vaccines to protect against H5N1 avian influenza Therapeutics for inhalation anthrax Discovery of novel causes of childhood respiratory infections Improved treatment of TB, including DR-TB Basic research on hepatitis C virus replication Department Name Goes Here 14

Currently studied BSL-4 agents at GNL Avian H5N1 Influenza Filoviruses (Ebola, Marburg) Bunyaviruses (RVFV, CCHFV) Arenaviruses (LASV, GTOV, JUNV, MACV, Lujo) Flaviviruses (CEEV, RSSEV, OHFV, KFDV, ALKV) Henipaviruses (NiV, HeV) 15

Services Divisions Services available to researchers in biodefense and emerging infectious diseases: Aerobiology Assay Development Experimental Pathology Imaging Insectaries Services Preclinical Studies 16

Species Currently Supported Mice Rats Hamsters Guinea Pigs Ferrets Rabbits Chinchillas Nonhuman Primates Avian Species Gerbils Insect Species Mosquito species: Aedes aegypti, Aedes albopictus, Culex pipiens, Anopheles gambiae Tick species: Amblyomma americanum Amblyomma cajennense Amblyomma imitator Dermacentor andersoni Dermacentor variabilis Dermacentor variabilis (Symbiont-free) Ixodes ovatus Ixodes pacificus Ixodes persulcatus Ixodes scapularis Rhipicephalus sanguineus (North Carolina strain) Rhipicephalus sanguineus (Israeli strain, pathogen-free) Rhipicephalus sanguineus (Israeli strain infected with Rickettsia conorii) 17

Specialized Equipment & Resources Equipment BSL2 BSL3 BSL4 FluoView 1000 Confocal Microscope Molecular Imager VersaDoc MP 4000 System Molecular Imager Gel Doc R System Personal Molecular Imager (PMI) System CereTom CT Scanner micropet Focus 220 PET Scanner IVIS Imaging System 200 Series Point-of-Care CR-IT 560 Digital -ray System TECAN Freedom EVO Canto Flow Cytometer TITAN Portable Ultrasound System InFlux Cell Sorter Ibis T5000 Universal Biosensor (ABSL) (ABSL) (ABSL) (ABSL) (ABSL) IVIS Imaging TECAN Robotics System Ibis T5000 Resources Aerobiology Facilities Good Laboratory Practices (GLP) Capabilities National Biocontainment Training Center Canto Flow Cytometer PET/CT 18

Multidisciplinary research themes Understanding disease mechanisms Host immune response Molecular biology of infectious agents Epidemiology Vector biology Ecology of the infectious agents Structure-function relationships of infectious agents Vaccinology 19

National Biocontainment Training Center (NBTC) Supported through a grant from the U.S. Department of Defense Collaborative effort of the safety professionals of the Environmental Health and Safety Office and the scientists of the GNL. Offers didactic and practical (mock lab) training to prepare scientists for work with infectious agents [BSL-2 to BSL-4 and ABSL-3] Two fellowship programs: Laboratory fellowship facilitate the development of select junior scientists in high containment infectious disease research Engineering fellowship develop high containment facility operation personnel 20

The National Biocontainment Training Center A national and international resource. To date, we have successfully trained more than 4,500 individuals to work in local, national and international BSL2, BSL3 and BSL4 labs. The NBTC offers 5 standard training courses. In addition to training laboratory staff at UTMB, the NBTC has trained staff from 40+ U.S. KEMRI universities, federal agencies and corporations. Internationally, the NBTC has trained staff from laboratories in 20+ countries including Argentina, Panama, Egypt, Israel and Taiwan. WHO The NBTC also offers a unique training and fellowship opportunities for biocontainment facility engineers. Department Name Goes Here NBL-RBL 21

Select Agent Facilities BSL-2 5,200 net ft 2 BSL-3 & ABSL3 29,713 net ft 2 BSL-4 & ABSL4 14,330 net ft 2 ~350 SRA-approved Employees [~100 Tier 1 Employees] FY12 Security Costs: > $4,000,000 Focus: Basic pathogenesis and discovery research Product development and evaluation GLP validation of vaccines and therapies 22

Key Contacts Director: James W. LeDuc, Ph.D. Scientific Director: Scott C. Weaver, Ph.D. Associate Director for Research & Operations: Joan Nichols, Ph.D. Assistant Director: Andrew G. McNees, Ph.D. Director of Containment Operations: Thomas G. Ksiazek, D.V.M., Ph.D. Director of Institutional Biocontainment Resources: Miguel A. Grimaldo Director of Strategic Initiatives: Alisha Prather Director of Information Technology: Bryan Fisher External Research Coordinator: Efthalia L. Bataki, Ph.D. Galveston National Lab Main: 409-266-6500 www.utmb.edu/gnl Nadezhda Yun, MD: neyun@utmb.edu 23