Immunotherapy of Uveal Melanoma



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Eye Am Not Alone (EANA) Patient Retreat Saturday Session - March 3, 2012 Immunotherapy of Uveal Melanoma Do not distribute or copy without the express permission of the Ocular Melanoma Foundation (OMF). These materials are for educational use only. No information provided herein constitutes a medical diagnosis, advice, or treatment. Please consult a healthcare professional for a specific examination and evaluation of your condition. Under no circumstances shall OMF nor the authors listed herein be held liable for anything that may arise from anyone following any advice, treatment, etc. included herein. Caroline Bosch-Voskens, MD Department of Dermatology University Hospital Erlangen

Caroline Bosch-Voskens, MD Caroline Bosch-Voskens is an Assistant Physician in the Department of Dermatology at the University of Erlangen in Germany. Caroline graduated from Medical School at Leiden University in her home country The Netherlands. During medical school, she conducted her scientific graduation project at the Wellcome Trust Research Laboratories, Blantyre, Malawi, Africa, in 2002. This project evaluated different techniques to diagnose hookworm as part of a large research project on aetiology, pathogenesis and long-term outcome on severe anemia in Malawian children. After graduating from medical school in 2004, she started to work as a research fellow at the University of Maryland, Baltimore, USA. Here, immuno-monitoring studies of a phase I clinical trial on peptide-based cancer vaccines were combined with basic laboratory studies on cellular immunotherapy. In 2009, Caroline returned to Europe and was awarded a training position in the department of Dermatology, University of Erlangen, Germany under supervision of Prof. G. Schuler. She is currently working as a resident and is involved in clinical studies on RNA transfected dendritic cells in melanoma patients. A large Phase III clinical trial using these dendritic cells as prophylactic treatment in uveal melanoma patients upon primary diagnosis is expected to start recruiting in the coming months.

Immunotherapy of uveal melanoma Learning to distinguish between friends and foes Eye Am Not Alone Patient Retreat, 03.03.2012, Washington DC Caroline Bosch-Voskens, MD Department of Dermatology University Hospital Erlangen

Diagnosis Treatment of the tumor in the eye Radiotherapy Surgery Wait and hope

What can I do once my cancer has spread? Local treatment Radiotherapy Surgery Systemic treatment Chemotherapy Kinase-Inhibitors (e.g. MEK-Inhibitors) Immunotherapy (e.g. Ipilimumab)

Immunotherapy - principle Teaching our own immune system to recognize and destroy existing tumor formations Teaching our own immune system to recognize and destroy solitary tumor cells in order to maintain a tumor-free situation Potential long-lasting therapy effect

Main characters of our immune system Killer cells release toxic particles, which kill unwanted cells (eg. bacteria, tumor cells) Dendritic cells provide killer cells with the necessary signals, which induce killer cell division and multiplication B cells produce so called antibodies, which directly destroy unwanted cells or indirectly activate killer cells

Immunotherapy of uveal melanoma Uveal Melanoma Uveal melanoma-specific Targets KILLERCELLS KILLERCELLS are always specific

Immunotherapy of uveal melanoma...... Uveal Melanoma Uveal Melanoma...

Natural immunity Every cancer patient already has some killer cells which actively recognize and kill unwanted cancer cells...nevertheless...they are hard to find... (11 million)

Immunotherapy - principle Changing the balance...

Immunotherapy - principle...into our favour!

Generation of killer cells Expansion of killer cells inside the body by cytokinetreatment Specific expansion of killer cells outside the body Specific expansion of killer cells inside the body through the use of your own helper cells (e.g. dendritic cells)

Therapy with cytokines Cytokines e.g. Interleukin-2; Interferon Treatment with cytokines induces non-specific expansion of killercells

Expansion of specific killer cells outside the body...and giving them back in large numbers after expansion (also called killer cell transfer )

Expansion of specific killer cells through the use of dendritic cells...by injecting dendritic cells in the proximity of lymph nodes or direct infusion in blood vessels

LYMPH NODE BLOOD VESSEL...........

Phase III Clinical Trial in monosomy 3 + uveal melanoma patients Goal To prolong disease-free survival in monosomy 3 + uveal melanoma patients by adjuvant treatment with autologous tumor-loaded dendritic cells Treatment starts right after first diagnosis Dendritic cells are loaded with your own specific uveal melanoma targets Study is IRB approved; funding is pending

Phase III Clinical Trial in monosomy 3 + uveal melanoma patients 7 days # 5 # 4 # 3 # 2 # 1

Antibodies Directly destroy unwanted cancer cells or indirectly activate killer cells by releasing the brakes of our immune system Ipilimumab (Yervoy ) First FDA approved antibody for the treatment of metastatic melanoma; releases the brakes of our immune system

When do I start Immunotherapy? The immune system needs time to get started Tumor growth may continue during the first weeks of treatment Minimal disease or a tumor-free situation are in general the ideal starting point Potential long-lasting therapy effect

What can I expect from Immunotherapy? Potential clearance of single tumor cells and/or destruction of small tumor formations In single cases destruction of large tumor formations Combined strategy of surgery/radiation and immunotherapy Prolonged tumor-free and/or overall survival

Currently approved Immunotherapies Cytokines for the treatment of skin melanoma (Interleukin-2; Interferon-alpha) Dendritic cells for the treatment of prostate cancer (Provenge ) Antibodies for the treatment of metastatic melanoma including uveal melanoma (Yervoy )

Common side-effects Fever Shivering Joint pains Skin reactions Itchiness Headache Tiredness Vitiligo Uveitis

Ipilimumab Associated with severe autoimmune reactions (60% of treated patients) Rapid and unexpected onset Side-effects are in general reversible by early recognition and prompt medical treatment Demands a high degree of awareness among patients and physicians

Clinical trials Studies recruiting uveal melanoma patients are increasing Studies specifically designed for uveal melanoma patients are increasing www.clinicaltrials.gov

Acknowledgements Department of Dermatology University Hospital Erlangen, Germany Prof. G. Schuler Dr. B. Schuler-Thurner Prof. E. Kämpgen Dr. M. Erdmann Ms. S. Rosenheinrich Ms. D. Schuster