CTRNet Standard Operating Procedure Administration of Standard Operating Procedures



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01.005 e2.1 CTRNet Standard Operating Procedure SOP Number: 01.005 Version: e2.1 Supersedes: 01.005 e2.0 Category: Administration Approved By: CTRNet Management Group (CMG) Per: Brent Schacter 01-May-2012 11-June-2012 1.0 PURPOSE Standard Operating Procedures (SOPs) are detailed written descriptions of how to execute a particular procedure or method. SOPs are based on national and international guidelines and conventions as well as policies and procedures that are considered best practice for the Canadian Tumour Repository Network (CTRNet) member biobanks. The purpose of having documented SOPs is to: Provide written guidelines for aspects of the CTRNet tumour biobank program. Promote quality and consistency in tumour biobanking and data collection across the CTRNet member biobanks. Ensure compliance with applicable regulations and guidelines. Facilitate education and training of biobank personnel. 2.0 SCOPE This Standard Operating Procedure (SOP) describes the processes for the development, review, approval and maintenance of all CTRNet written SOPs. It applies to all tumour biobank personnel involved in writing, revising, reviewing, approving and maintaining SOPs. 3.0 REFERENCE TO OTHER CTRNET SOPS OR POLICIES Note: When adopting this SOP for local use please reference CTRNet. 3.1 CTRNet Policy: POL 5 Records and Documentation 3.2 CTRNet Policy: POL 3 Education and Training 3.3 CTRNet Standard Operating Procedure: SOP 07.001 Education and Training Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 1 of 13

01.005 e2.1 4.0 ROLES AND RESPONSIBILITIES This SOP applies to all tumour biobank personnel involved in writing, revising, reviewing, approving and maintaining SOPs. Tumour Biobank Personnel Consultant Laboratory Technician/Technologist Assistant/Clinical personnel Tumour Biobank Manager SOP Review team Responsibility/Role Writing, Revising and updating technical and organizational SOPs Writing, Revising and updating technical SOPs Writing, Revising and updating organizational and administrative SOPs Reviewing, Revising and updating technical and organizational SOPs 5.0 MATERIALS, EQUIPMENT AND FORMS The materials, equipment and forms listed in the following list are recommendations only and may be substituted by alternative/equivalent products more suitable for the site-specific task or procedure. Materials and Equipment Materials and Equipment (Site Specific) SOP Format document 6.0 DEFINITIONS See the CTRNet Program Glossary: http://www.ctrnet.ca/glossary 7.0 PROCEDURES SOPs are controlled documents designed to give instructions for performing routine and essential processes, to ensure that they are performed consistently and in a manner upholding CTRNet program quality and integrity. 7.1 Developing New SOPs or Revising Previously Issued SOPs 7.1.1 CTRNet, management at the regional biobanks or biobank personnel can identify the need for new/revised SOPs. The need can arise from the findings of a routine SOP review or from changes to regulations, guidelines, research practice, or institutional policies. 7.1.2 Individuals well versed with the procedures or methods being described should be recruited to draft or assist in drafting a new or revised SOP. 7.1.3 The SOP should follow the standard format (see SOP template, Appendix A); the word draft should be added to the header. For major revisions to previous SOPs, the major SOP version number must be incremented by one (e.g., 1.0 becomes 2.0). For minor revisions to previous SOPs, the minor version number must be incremented by 1(e.g. 1.0 becomes e1.1). The first version of an SOP is always 1.0. 7.1.4 Develop/revise associated attachments, as applicable and revise the version date. Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 2 of 13

01.005 e2.1 7.1.5 Update the SOP index (see Attachment B) as necessary (e.g., for new SOPs added). 7.2 Review and Approval of SOPs 7.2.1 Circulate the draft SOP to the applicable reviewers (SOP working committee, management at local biobanks, biobank personnel, and other identified staff representatives e.g., SOP users) for comments. 7.2.2 Incorporate the comments, revise the draft version date and circulate the revised draft SOP to the biobank Director or designate. 7.2.3 Review the final draft SOP for accuracy and completeness and for compliance with regulations, guidelines and standard practice. In some circumstances, this may involve REB approval of amendment. Also, should note whether change in SOP will require amendment to other administrative documents, especially any applicable Privacy Impact Assessments. 7.2.4 Obtain approval (from a SOP authorized signatory) of the final SOP. 7.2.5 Add the effective date to the front page (the date that the final signed-off SOP is scheduled to be implemented). Remove draft from the header. 7.3 Format and Content of SOPs 7.3.1 Write the SOP using the formatting and styles (e.g. Arial) as shown in the standard SOP template (Appendix A). 7.3.2 Complete the heading and footer information as shown in the SOP Template. 7.3.3 The effective date refers to the date that the approved SOP is to be implemented. 7.3.4 An SOP index should be created to list all of the approved SOPs, separating them into logical categories (Attachment B). The example below separates the SOPs into categories corresponding to the general flow of the Tumour Biobank operations. Combining the abbreviated SOP category with the series number creates the SOP number. The original list of SOPs may contain gaps in the numbering sequence, in order to accommodate new SOPs in logical order. Standard Operating Procedure Categories and Numbering System SOP Category Category (xx).sub-category (xx).sop Number (xxx) General Institutional Requirements of a Biobank 00.000 Administration 01.001 Participant Recruitment and Management 02.001 Records Management and Documentation 03.001 Facilities Management and Operations 04.001 Quality Assurance 05.001 Safety 06.001 Training 07.001 Material Handling and Documentation 08.01.001 Materials Request and Release 09.001 Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 3 of 13

01.005 e2.1 7.3.5 For new SOPs, assign the next consecutive number in the appropriate category. The SOP version number for each original (new) SOP will be e1.0 (English) or f1.0 (French). 7.3.6 Divide the content into sections as shown in the SOP Template (Appendix A). 7.3.7 For revisions to previously issued SOPs, include a summary of and rationale for the revision in Section 10. 7.3.8 Although SOP attachments may be reviewed, revised and approved separately from the SOP, they should be stored with the applicable SOP. 7.3.9 The SOP index may also contain sub-categories if necessary, as shown above for category 08 Material Handling and Documentation. 7.4 SOP Maintenance 7.4.1 Institute a review process for SOPs. 7.4.2 SOPs should be reviewed regularly (see Appendix B for suggested review schedule). The SOPs should be reviewed sooner if there are changes to regulations, guidelines, research practice, or institutional policies. 7.4.3 Once an SOP is reviewed, complete the SOP Review Record (Appendix C) file copy with CTRNet and regional biobank central office. 7.4.4 If revisions to an SOP are required, follow the review and approval process (See Section 7.1). 7.4.5 If only revisions to an attachment are needed, modifications may be made without revising the SOP. Revise the attachment, update the version date, and file a copy with CTRNet and the regional biobank central office. 7.5 SOP Distribution and Communication 7.5.1 SOPs should be readily available to all tumour biobank personnel and other identified staff users. 7.5.2 Notify all biobank personnel, management members of the biobank, and other identified staff users of any new or revised SOPs, and the rationale for the SOP or SOP changes. Ideally, direct users should be notified immediately of new/revised SOPs. 7.5.3 Provide training on new or revised SOPs. Document training as appropriate to meet regional or institutional requirements. 7.5.4 Retrieve outdated copies of SOPs and attachments and replace with updated versions. 7.5.5 Outdated SOPs, appendices and SOP indices should be archived. Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 4 of 13

01.005 e2.1 7.6 SOP Storage 7.6.1 The CTRNet and the regional tumour biobank should create and maintain a central SOP file. 7.6.2 Store the following documents in the central SOP files: SOP Distribution Records (Appendix D) or electronic audit trail if relevant. Final, approved original and revised versions of each SOP. One copy of the original and revised versions of each SOP appendix. Original, signed SOP Review Records. Copies of SOP training records from the collection sites (if maintained) 7.6.3 For electronic SOPs, final SOPs should be posted in a format that cannot be altered (e.g.,.pdf format). Ensure that the electronic files are checked regularly and only current SOPs are referenced. 7.7 SOP Style 7.7.1 Describe each operation in a procedure as a separate step. Make instructions explicit enough so that a qualified individual could perform the procedure by following the instructions. Make instructions explicit enough so that the SOP may be used as a training tool, and easily referred to for guidance during routine work. 7.7.2 Use clear, concise, unambiguous instructions so that the user can understand the requirements. Do not use qualifiers and vague terms such as usually, sometimes, normally, regularly or try to. 7.7.3 Flow charts may be included, as they are an excellent way of communicating the sequential steps of a process. Equipment diagrams and scanned images can also help personnel understand machinery, and are useful aids during hands-on training sessions. 8.0 APPLICABLE REFERENCES, REGULATIONS AND GUIDELINES 8.1 Declaration of Helsinki http://www.wma.net/en/30publications/10policies/b3/index.html 8.2 Tri-Council Policy Statement 2; Ethical Conduct for Research Involving Humans; Medical Research Council of Canada; Natural Sciences and Engineering Council of Canada; Social Sciences and Humanities Research Council of Canada, December 2010. http://www.pre.ethics.gc.ca/eng/policy-politique/initiatives/tcps2-eptc2/default/ 8.3 Human Tissue and Biological Samples for use in Research. Operational and Ethical Guidelines. Medical Research Council Ethics http://www.mrc.ac.uk/utilities/documentrecord/index.htm?d=mrc002420 9.0 APPENDICES 9.1 Appendix A SOP Standard Template 9.2 Appendix B SOP Index 9.3 Appendix C SOP Review Record 9.4 Appendix D SOP Distribution Record Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 5 of 13

01.005 e2.1 10.0 REVISION HISTORY SOP Date Number revised Author Summary of Revisions 1.1.005 09-01-2008 JdSH Original Document 1.1.005 July 2011 MMA Section 8: TCP2 referenced, links updated Appendix A: Header generalized Appendix B: revision period changed to 2 year minimum, SOP 8.02.006 added. Appendix C: SOP review record updated Appendix E: SOP deviation report - added 1.1.005 e2.0 May 2012 CMG Grammatical and formatting throughout Definitions removed Revision History moved to bottom Reference links updates Updated SOP references Revised 7.3 format and content of SOPs Revised appendices Revised section 7 to reflect new SOP numbering system Deleted Appendix E (Deviation Report) there is no reference to this appendix in this SOP Deleted 7.3.6 For revised SOPs, revise the version number (e.g.,. e1.1 or f1.1 for the first revision). This is already mentioned in 7.1.3. 01.005 e2.0 June 12, 2012 AJS BAS Section 7.3.4 table: added sub-category to the heading and added the sub-category # to section 8 SOPs in the table. Added line 7.3.9 Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 6 of 13

01.005 e2.1 APPENDIX A Standard Sop Template DRAFT STANDARD SOP TEMPLATE SOP Number: Supersedes: CTRNet Standard Operating Procedure TITLE Version: Category: Approved By: CTRNet Management Group Name DD/MM/YYYY Formatting should follow the formatting and numbering used in this template (e.g., Arial 12 font for headings, and Arial 10 font for body text). Abbreviations should be written out in full the first time they appear, followed by the abbreviation in brackets. SOPs should be clear, concise and not overly narrative. 1.0 PURPOSE Provide a brief purpose of the standard operating procedure (SOP). 2.0 SCOPE A general statement of what is covered by the SOP (what processes the SOP describes). 3.0 REFERENCE TO OTHER CTRNET SOPS OR POLICIES 3.1 Cross-reference (list) all SOPs relevant to this SOP (Italics). 4.0 ROLES AND RESPONSIBILITIES Indicate who is responsible for the procedures and list personnel that the SOP applies to. Tumour Biobank Personnel Responsibility/Role Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 7 of 13

5.0 MATERIALS, EQUIPMENT AND FORMS 01.005 e2.1 APPENDIX A Standard Sop Template The materials, equipment and forms listed in the following list are recommendations only and may be substituted by alternative/equivalent products more suitable for the site-specific task or procedure. Materials and Equipment Materials and Equipment (Site Specific) 6.0 DEFINITIONS See the CTRNet Program Glossary: http://www.ctrnet.ca/glossary 7.0 PROCEDURES Describe the tasks required to implement each activity, breaking them into logical categories. Include any documentation requirements. Start with a short preamble to introduce the section, if appropriate. 7.1 Heading of First Category (Bold, Arial 10 font) Provide a step-by-step list of tasks/procedures. Include bullet points for additional information related to a step/task. e.g., associated documents 7.1.1 Sub-Sections as required a. Include alpha points for additional information related to step/task. 7.2 Heading of Second Category (Bold, Arial 10 font) 7.2.1 Sub-sections 8.0 APPLICABLE REFERENCES, REGULATIONS AND GUIDELINES List the applicable regulations and guidelines the SOP is governed by. Include current Internet link/url where possible. 8.1 FDA CFR 8.2 ICH / GCP 8.3 Tri Council Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 8 of 13

01.005 e2.1 APPENDIX A Standard Sop Template 9.0 APPENDICES Appendices are usually revised more frequently than an SOP and can be modified without revising the entire SOP. List associated attachments (i.e., forms, checklists) The attachments are not part of the official SOP document Attachments should include a document name, version date, page X of X in the footer 9.1 Appendix A 9.2 10.0 REVISION HISTORY SOP Number Date revised Author Summary of Revisions Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 9 of 13

01.005 e2.1 APPENDIX B Standard Operating Procedures Index STANDARD OPERATING PROCEDURES INDEX SOP Number SOP CATEGORY/ Title Date Approved Review scheduled 00 General Institutional Requirements of a Biobank 00.001 Organizational Chart N/A 00.002 Job Descriptions N/A SOPs should be reviewed no less than every 2 years 01 Administration 01.001 Obtaining Confidentiality Disclosure Agreements 29-May-2012 May 2014 01.004 Handling Participant (Donor) Complaints 29-May-2012 May 2014 01.005 29-May-2012 May 2014 01.006 Job Descriptions, Roles, and Responsibilities 02 Participant Recruitment and Management 02.001 Participant Recruitment into Tumour Biobank Program 29-May-2012 May 2014 02.002 Developing and Revising Consent Forms 29-May-2012 May 2014 02.003 Requesting Additional Survey Information 29-May-2012 May 2014 02.005 Obtaining Informed Consent 29-May-2012 May 2014 02.006 Withdrawal of Consent 30-May-2012 May 2014 02.007 Notification of Significant and Relevant Findings 30-May-2012 May 2014 03 Records Management and Documentation 03.001 Information Access Control (Privacy and Security) 30-May-2012 May 2014 03.002 Database Back-up Systems (Privacy and Security) 31-May-2012 May 2014 03.003 Data Transmission to CTRNet Catalogue 31-May-2012 May 2014 03.004 Clinical Annotation 31-May-2012 May 2014 03.008 Document Maintenance 31-May-2012 May 2014 04 Facilities Management and Operations 04.001 Physical Security at Facilities 31-May-2012 May 2014 04.004 Emergency Procedure for Freezer and Refrigerator Failure 31-May-2012 May 2014 04.006 Maintenance of Sample Storage Facility and Equipment 31-May-2012 May 2014 05 Quality Assurance 05.001 Assessing Quality of Tissue Specimens 31-May-2012 May 2014 05.002 Assessing Quality of Nucleic Acids 31-May-2012 May 2014 06 Safety 06.002 Handling Hazardous Chemical Waste 31-May-2012 May 2014 06.003 Immunization and Personnel Accident Reports 01-June-2012 June 2014 07 Training 07.001 Education and Training 01-June-2012 June 2014 08 Material Handling and Documentation 08.01 Material Handling and Documentation - General Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 10 of 13

01.005 e2.1 APPENDIX B Standard Operating Procedures Index 08.01.001 General - Labeling and Tracking Materials 01-June-2012 June 2014 08.01.002 General - Biohazardous Material Waste Management 01-June-2012 June 2014 08.01.003 General - Inventory Verification 14-June-2012 June 2014 08.02 Material Handling and Documentation - Blood 08.02.001 Blood - Blood Collection 13-June-2012 June 2014 08.02.002 Blood- Blood Processing and Storage 13-June-2012 June 2014 08.02.003 Blood - Blood Derivatives Extraction of RNA 13-June-2012 June 2014 08.02.004 Blood - Blood Derivatives Extraction of DNA 13-June-2012 June 2014 08.02.005 Blood - DNA Extraction from Blood 26-June-2012 June 2014 08.03 Material Handling and Documentation Solid Tissue 08.03.001 Solid Tissue - Tissue Collection and Transportation 26-June-2012 June 2014 08.03.002 Solid Tissue - Tissue Harvesting 26-June-2012 June 2014 08.03.003 Solid Tissue - Snap Freezing of Tissue 28-June-2012 June 2014 08.03.004 Solid Tissue - Freezing of Tissue in Optimal Cutting 28-June-2012 June 2014 Temperature 08.03.005 Solid Tissue - Preservation of Tissue Parrafin 28-June-2012 June 2014 Embedding 08.03.006 Solid Tissue - Sectioning of Tissue Paraffin and OCT 28-June-2012 June 2014 Embedded Tissue 08.03.007 Solid Tissue - Haematoxylin & Eosin Staining of Tissue 26-June-2012 June 2014 Sections 08.03.008 Solid Tissue- Tissue Derivatives Extraction of DNA 28-June-2012 June 2014 08.03.009 Solid Tissue - Tissue Derivatives Extraction of RNA 28-June-2012 June 2014 08.03.010 Solid Tissue - Tissue Micro Arrays from Paraffin 28-June-2012 June 2014 Embedded Blocks 08.03.011 Solid Tissue - Sample Retieval 28-June-2012 June 2014 09 Materials Request and Release 09.001 Sample Shipping and Transportation 28-June-2012 June 2014 09.002 Completion of an MTA (Material Transfer Agreement) 28-June-2012 June 2014 09.004 Material Request and Release 28-June-2012 June 2014 Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 11 of 13

01.005 e2.1 APPENDIX C SOP Review Record SOP REVIEW RECORD Name of reviewer: Contact email: Review date: SOP Number: SOP Title: Section Page Number Comments and description of change Additional comments: INSTRUCTIONS: Please read the assigned SOP and review for any needed updates. Type comments and any applied changes into the table above, adding additional rows as needed. Save as CTRNet SOP review NUMBER_NAME OF SOP, DATE. Use 1 form per SOP. Edit the SOP with TRACK CHANGES. If no updates are needed, please state so in the additional comments section. Return all forms and track-changed SOP by email to [insert name of review coordinator and email address] Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 12 of 13

01.005 e2.1 APPENDIX D SOP Distribution Record SOP DISTRIBUTION RECORD This template can be used at CTRNet, regional Tumour Biobank office (Director or designate) as well as the local collection sites (Principle Investigator) to track distribution / communication of new/revised SOPs. Electronic audit trail should also be attached to this form. NEW / REVISED SOP (Number.Version) EFFECTIVE DATE (DD/MMM/YY) COMMUNICATED TO USERS (Attach supporting documentation) (DD/MMM/YY) COMMUNICATED BY (Name) TRAINING COMPLETED (DD/MMM/YY) Copyright 2012 Canadian Tumour Repository Network (CTRNet). Page 13 of 13