ASFA Meeting 2015 San Antonio Robin Willis RN BSN HP. Red Cell Exchange Overview
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1 ASFA Meeting 2015 San Antonio Robin Willis RN BSN HP Red Cell Exchange Overview
2 No Disclosures Conflict of Interest
3 Overview Indications for Red Cell Exchange Procedural Goals Vascular Access Case Studies Isovolemic Hemodilution
4 Indications for RBCX 1. Sickle cell disease - red cells are defective and cause vaso occlusion 2. Thalassemia - deficiency of alpha globin or beta globin 3. Parasitic infections such as malaria or babesiosis 4. Carbon monoxide poisoning 5. Incompatible transfusion
5 Sickle Cell Disease Most common inherited blood disorder in USA Affects approx. 75,000 people in USA Abnormal hemoglobin causes crescent shaped red cells. Hemoglobin S (HB S) is present instead of Hemoglobin A (HB A) Signs/symptoms include anemia, SOB, jaundice, painful episodes
6 Thalassemia In alpha thalassemia, the alpha globin genes have a mutation or abnormality In beta thalassemia, the beta globin genes are affected Transfusions are required to maintain hct to support tissue oxygenation Prevalent in Mediterranean, Indian, Middle Eastern populations
7 Parasitic Infections Malaria- spread by mosquitoes infected with the Plasmodium Falciparum parasite - intraerythrocytic parasite (no longer indicated by the CDC) Babesiosis- spread by ticks infected with the protozoan parasite Babesia intraerythrocytic parasite
8 Carbon Monoxide Poisoning Carbon Monoxide has an affinity for the heme molecule RBC s become saturated with CO Signs/Symptoms most common symptoms - headache, dizziness, weakness, upset stomach, vomiting, chest pain, and confusion RBCX - removes the carbon dioxide saturated red cells and replaces with healthy blood donor cells
9 Incompatible Transfusion Example Rh + red blood cells are transfused to Rh - recipient Goal - Remove Rh incompatible cells are removed from the recipient
10 Procedural Goals Removal of unhealthy cells with simultaneous replacement of healthy donor red blood cells Diminish pain episodes Stabilize hematocrit Improve respiratory status Maintain even fluid balance
11 Red Cell Exchange Most commonly performed for Sickle Cell Disease Urgent exchange - acute chest syndrome, declining respiratory status, stroke, preoperatively, stroke prevention Maintenance schedule- exchange is performed every 3-4 weeks Goal - maintain a reduced % of Hgb S, correct anemia and reduce transfusional iron overload
12 Reduced Ferritin Levels Our experience illustrates the effectiveness of utilizing red blood cell exchanges to effectively reduce iron overload in some patients with sickle cell disease. Red blood cell exchanges appear to be an important therapy to consider in the overall management of iron overload in sickle cell disease patients.
13 Sickle Cell Disease This depicts sickled cells causing vaso occlusion in blood vessels Vaso occlusion causes hypoxia and pain Risk of infection is increased, cells are sticky Hypoxia in kidneys, lungs, spleen and brain requires immediate medical intervention Image Courtesy of NIH.gov
14 Complications of SCD As the cells sickle and congregate in the vessel, blood flow is blocked Vaso occlusive events can lead to: Stroke Acute chest syndrome MSOF (Multi System Organ Failure) Cholestatic Crisis (Bilirubin over 50)
15 Managing Sickle Cell Crisis Oxygen therapy to improve respiratory status IV fluids for hydration Antibiotics for potential/actual infection Opioids for pain management Red blood cell transfusion for anemia
16 Indications for RBC Exchange Declining respiratory status/ WOB Anemia Increased percentage of HgS Pain crisis
17 Vascular Access Peripheral Draw line 16 or 17 GA fistula needle Return Line 18GA 20GA angiocatheter Central Central Venous Catheter (CVC) -Double Lumen Apheresis Catheter Port - Vortex double lumen or single lumen port (one on each side of upper chest)
18 DL Central Venous Catheters Guidelines for Apheresis Treatments Temporary Femoral Medcomp 7F x 10 cm Pt 25 kg Medcomp 9 F x 12 cm Pt 25 kg Temporary Subclavian/Internal Jugular Mahurkar 8F x 12 cm (smaller pts) Quinton 8 F x 15 cm (smaller pts) Mahurkar 10 F x 15 cm (small adults) (Quinton) 10 F x 19.5 cm (small adults) Mahurkar 11.5 F x 13.5 cm (adult pts) (Quinton) 11.5 F x 16 cm (adult pts) 11.5 F x 19.5 cm (adult pts)
19 Determine Volume of RBC s Required Patient s sex, height, weight Patient s Hct ( must have an accurate Hct) Hemodilution (RBC Depletion) volume of RBC s required will be reduced Desired Hct at end of RBCX Average Hct of replacement rbc units Fluid balance Fraction of Cells Remaining (FCR)
20 What is FCR? FCR is the percentage of the patient s original RBCs remaining in the patient at the end of the procedure 100% patient HbS RBC RBCX 80% donor HbA RBC 20% patient HbS RBC = FCR% Courtesy of TerumoBCT.Inc
21 Laboratory Tests Accurate Hematocrit is essential! Hemoglobin Electropheresis pre and post exchange (analyzes the various types of Hemoglobin i.e. Hgb S, Hgb A, Hgb F Post exchange CBC Ionized Calcium pre and post exchange (Hypocalcemia caused from ACD-A anticoagulant and Citrate in the RBC s
22 Hcts of Replacement Units Order Red Blood Cells from the Blood Bank Obtain a sample from each of the Replacement Units to determine Hct of each Unit Calculate the average Hct of Replacement Units For washed units, Hct is approximately 70%
23 Transfusion Reaction Signs/Symptoms Urticaria Itching Chills Fever Flank Pain Bloody Urine Rapid, thready pulse Fainting/dizziness Nursing Actions STOP transfusion Contact MD/NP Prepare to administer Acetaminophen, Antihistamine Maintain access/prepare to administer IV fluids Complete Transfusion Reaction Report/blood samples, Urine samples/recheck component tags for accuracy/send tubing to Blood Bank for analysis Continue to monitor vital signs
24 Determining RBC s Volume Required Use data entry screen apheresis machine to determine volume of RBC s required If pt is hemodynamically unstable or is 25 kg, prime machine with a unit of RBC s Remember to include this additional unit in the volume of RBC s required
25 Do Not Rinseback! Do not rinseback at the end of a RBC exchange. The predicted end Hct and FCR do not calculate for rinseback If rinseback is done, then post Hct and FCR will likely be higher than the targeted values
26 Isovolemic Hemodilution Two part process in the RBC Exchange 1 st Phase - RBC Depletion- red blood cells are removed with isovolemic replacement with 0.9% NaCl to reach the desired Hct 2 nd Phase Immediately following the RBC Depletion, RBC Exchange is initiated replacing with RBC s
27 Advantages of Isovolemic Hemodilution Reduces the volume of RBC s required for the RBCX Increases the efficiency of the RBCX by promoting further reduction of the Hgb S May increase the time interval between scheduled RBC exchanges
28 Case Study #1 Male, 5 10 (178 cm) 75 kg (TBV 5082 mls) Hct 26% End hct 30% Average hct of replacement units is 62% FCR 25% Fluid balance 100% Replace volume = 3106 mls Replace volume with hemodilution = 2861mLs
29 Case Study #2 15 yo male pre op for hip surgery (AVN). RBCX requested to reduce Hgb S (80.8%) 45kg hct 28%, 157 cm, TBV = 3469 mls Data Entry - FCR 25% Avg replace unit 62%. Desired post rbcx Hct 30%. Replace volume= 2183 mls Using hemodilution erythrocyapheresis method- replace volume is estimated to be 1981 mls Right Internal Jugular 11.5 FR. Tip confirmed to be in the right atrial SVC junction Replaced with 2 grams 10% Calcium Gluconate infused to return line Anticoagulant: ACD-A Ratio 1:13
30 Review of Case Study # 2 Post RBCX Flow rates mls/minute Run time 92 minutes Replace volume 2183 mls Hct 30.0% Hgb S 21.4% Post RBC exchange - FCR 26.5% Plt count reduced by 59%
31 Case Study #3 3 yo 20.2 kg female with increased WOB/chest pain/abdominal pain. RLL infiltrate, hypoxia Hct 22.7% HgbS 46.1% Temporary (non tunneled) Left Subclavian Apheresis Catheter 7.0 FR, tip confirmed to be in upper SVC
32 Calculating TBV For patients less than 25 kg, the operator calculates the TBV rather than the apheresis instrument Use the recommended conversion formula at your institution. Examples of conversions are below: Neonates: 90 mls/kg Infants: 80 mls/kg Others: 70 mls/kg Example: 70 mls/kg Example 20.2 kg x 70 = 1414 mls
33 Benefits of Blood Priming Maintains hemodynamic stability Maintains isovolemia
34 Calculation of Pt s Hct after Blood Priming Apheresis Circuit Red cell volume Whole blood volume Example: TBV = 4200 mls, patient s hct is 26% Pt s RBC volume = 1092 mls Priming Unit hct is 62%, volume of unit is 240 mls (240 x.62 = 149 mls) Apheresis circuit volume is 325 mls 1092 mls +149 mls 4200 mls+240mls = 27.95% (pt s estimated hct after prime)
35 Case Study #3 Pt s Hct after Blood Prime 20.2 kg x 70 mls/kg = 1414 mls (pt s TBV) Patient s TBV = 1414 mls, patient s hct 22.5% Formula to calculate patient s hct after blood prime (Patient s RCV) + (RCV of apheresis circuit ) (Patient s TBV+ Volume of apheresis circuit) or simply RCV WBV Hct of priming unit = 60% Pt s RCV= 1414 mls x.225 (pt s hct) = 318 mls RCV of priming unit (240 mls x.60) = 144 mls 318 mls mls 1414 mls mls (apheresis circuit + blood warmer 285 mls + 40mLs) = 26.5% Patient s Hct after blood prime = 26.5%
36 Data Entry Case Study # 3 Female, TBV 1414 mls Hct after prime is 27% Average Hct of Replacement Units 60% Desired End Hct 30% Fluid Balance: 100% FCR: 25% Estimated RBC replace volume required is 872 mls Remember to request an additional unit to prime the apheresis machine (240 mls) Total = 1112 mls
37 Thank You!
38 Acknowledgements Apheresis Nurses Cheryl Pacheco Phaedra Truglia Lauren Salter Jennifer Strykowski Jill Dattilio Physicians John Manis Steven Sloan Rick Kaufman Li Chai William Savage
39 Questions
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