Therapeutic Systems Immunology

Transcription

1 Therapeutic Systems Immunology Coordination: Robert Preissner, Charité, Berlin 13 Partners from Berlin (7) Göttingen (1) Heidelberg (1) Rostock (2) Tübingen (1)

2 Overview Intro / Mission Partners Systems Biology Berlin, Heidelberg, Göttingen Clinic Charité: Urology, Oncology, Pediatrics Immunology Rostock, Tübingen Companies Examples Epitope Mapping Structure Based Peptide Libraries Tumor Therapy Mimotopes Acknowledgements

3 The immune system (IS) Network of processes Protection against disease By identification of Pathogens Tumor cells Innate and adaptive IS Here: focus on adaptive (specific) IS Major players: MHC/T cell receptors and antibodies

4 Mission Immunology Cross reactivity Non linear epitopes Clinic (urological tumors) Tumor specific antibody diagnostics Tumor specific therapy Elimination of harmfulauto antibodies Induction of therapeutic antibodies + T cell stimulation Systems Biology Holistic model of tumor directed immune response

5 Systems Biology Clinic Bork, EMBL Pathways, Signaling Grubmüller, MPI MHC affinities Preissner, Charité Cross reactivity Non linear Epitopes Lode, Pediatrics DNA vaccination Schrader, Urology Sera, Tissue Arrays Daniel, Oncology Cell Biology

6 Companies Immunology JPT Peptide Arrays IndyMed Epitope Profiling Mologen Renal Cancer Drugs DNA based Therapeutics ERDE Antibody Diagnostics Thiesen, Rostock B cells Rammensee, Tübingen T cells Höpfner, Berlin Growth Factors, Signaling

7 Integration of Systems Biology and Clinic Systems Immunology Model of immune response Crossreactivity and nonlinear epitopes Diagnostics Peptide arrays antibody detection Tumor model Therapy Mimotope vaccination Antibody induction Antibody elimination Therapy Antibody antigen combination

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10 Milestones Property patterns of epitopes Immunological fingerprints of urological tumors Diagnostic arrays (detection and prognosis) Therapeutic antibodies Understanding (prediction) of cross reactivity Model (simulation) of immune therapy Peptides for immune adsorption

11 Overview Mission Partners andtopics Systems Biology Berlin, Heidelberg, Göttingen Clinic Charité: Urology, Oncology, Pediatrics Immunology Rostock, Tübingen Companies Examples Epitope Mapping Structure Based Peptide Libraries Tumor Therapy Mimotopes Acknowledgements

12 (Re )construction of a protein protein recognition site Antigen Antibody Non linear epitope (three segments) PDB Code: 1YQV

13 Surface simulation scan: structure-based peptide libraries Idea: combine peptide segments that are adjacent in space (but not in sequence) Features Linear and non-linear epitopes Additional disulphide bonds Regions (automatic or user def.; e.g. around ligands or interfaces) Linkers from PDB (adapted from LIP) Protein surface mapping via structure based peptide library design Goede, A. Jaeger, I. and Preissner, R. BMC Bioinformatics 9;6:223

14 SuperFicial

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17 Successful reconstruction of a non linear antigen Translation of the protein surface into a peptide library Zeile Spalte Peptid NRNTDGSTD PG NrR PGF RrPG NRNTDGSTD PG NrR PGA RrPG NRNTDGSTD PG NiRPGF RcPG NRNTDGSTD PG NiRPGA RcPG Incubation with prim. Ab and sec. Ab (anti mouse IgG, peroxidase label) Black spots: binding The binding was validated by Biacore experiments with soluble peptides (not shown) r, i, c = inserted Cysteines to establish disulfide bonds (stabilising the desired conformation) Proof of principle! application to a number of protein pairs under way

18 Overview Mission Partners andtopics Systems Biology Berlin, Heidelberg, Göttingen Clinic Charité: Urology, Oncology, Pediatrics Immunology Rostock, Tübingen Companies Examples Epitope Mapping Structure Based Peptide Libraries Tumor Therapy Mimotopes Acknowledgements

19 Vaccination with tumor markers Membrane Tumor marker Antibody

20 Immune therapy via cross reactivity Antibody 1 Therapeutic Antibody 2 Marker Mimicry Peptide Marker Why look for mimetics? Markers are weak Antigens!

21 Translation of the marker into a peptide Peptide MA Peptide MD Peptides were optimized und validated: Solid phase synthesis Biacore Animal trials Successful Cancer Research 66: Mol Cancer Ther. 7: For further tumors under way!

22 SUMMARY The IS may cause cancer (development) but may also provide the cure! Protein surfaces (non linear epitopes) can be translated into peptide libraries Thiscanbeusedfor diagnostics (identify antibodies) therapy eliminate harmful auto antibodies induce therapeutic antibodies

23 Acknowledgements I would like to thank: my partners, P.M. Schlag (CCCC) for providing a central lab and (esp. Dr. Schug) for support and patience

24 Simplified Scheme regarding SPOT Synthesis Aminosäure Peptid Linker Linker Zellulose Membran mit Spots Peptid Linker Protein Markierung About 5 10,000 peptides per array (1 entire protein surface translates into about 2,000 peptides)

25 Schedule II