Portunidae 幷 [1] Genetic diversity. * 2006BAD03B Z M

Transcription

1 Mar. 2010, Volume 4, No.2 (Serial No.27) Journal of Life Sciences, ISSN , USA 幷 1. 幷 [1] [2] [3] [4] Scylla spp. Crustacea Decapoda Brachyura Portunidae 1. Genetic diversity * 2006BAD03B Z M kjjiang@hotmail.com malingbo@vip.sina.com. 45

2 Molecular marker DNA Genetic marker DNA [5] DNA [6] 3. 9 [7] Restriction Fragment Length Polymorphism RFLP [8,9] DNA Random Amplified Polymorphic DNA RAPD [10] Amplified Fragment Length Polymorphism AFLP [11] DNA Simple Sequence Repeat SSR [12-14] Inter-Simple Sequence Repeats ISSR [15] DNA Single-Nucleotide Polymorphism SNP [16] DNA Mitochondrial DNA mtdna [17] Expressed sequence Tag, EST [18] Sing1e Strand Conformation Po1ymorphism SSCP DNA DNA Amplification Fingerprinting DAF Denaturing Gradient Gel Electrophoresis DGGE Temperature Gradient Gel Electrophoresis TGGE 4. DNA S. serrata [19] S. serrata, S. oceanic, S. tranquebarica [20] S. serrata, S. tranquebarica, S. paramamosain, S. olivacea [21] DNA 幷 DNA DNA DNA COI [22] 12 46

3 COI 98% 9.98% 10.84% [23,24] DNA Gopurenko 3 N=21 3 N=76 5 N=28 S. serrata [25] I I II 38 Numts [26] COI [27] 5 [28] 10 [29] [30] [31,32] [33] 1 [34] DNA PCR

4 幷 DNA [24,35] DNA 16.8% [36] [37] RAPD RAPD 3 [38] DNA [39] [40] [41] 15% 48

5 [42] [43-45] [46-49] 7. 幷 [1]. [J] [2]. [J] [3]. [J] [4]. [J] [5]. [J] [6]. [J] [7]. [J] [8] Botstein, D., White, R. L., Skolnick, M., et al. Construction of a genetic linkage map in man using Restriction Fragment Length Polymorphisms. American Journal of Human Genetics, 1980, 32: [9] Grodzicker, T., Anderson, C., Sharp, P. A., et al. Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids I. host range mutants of Ad2+ND1. Journal of Virology, 1974, 13: [10] Williams, J. G. K., Anne, R., Livak, K. J., et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic makers. Nucleic Acids Research, 1990, 18: [11] Zabeau, M. and Vos, P.. Selective restriction fragment amplification: A general method for DNA fingerprinting. European Patent Application (Publication No A1) Paris: European Office, [12] Moore, S. S., Sargeant, L. L., King, T. J. et al. The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics, 1991, 10: [13] Rassmann, K., Schlötterer, C. and Tautz, D.. Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting. Electrophoresis, 1991, 12: [14] Schlotteröer, C., Amos, B. and Tautz, D.. Conservation of polymorphic simple sequence loci in cetacean species. Nature, 1991, 354: [15] Zietkiewicz, E., Rafalski, A. and Labuda, D.. Genome fingerprinting by Simple Sequence Repeat (SSR)-anchored Polymerase Chain Reaction amplification. Genomics, 1994, 20: [16] Landegren, U., Nilsson, M. and Kwok, P.-Y. Reading bits of genetic information: methods for Single-Nucleotide Polymorphism Analysis. Genome Research, 1998, 8: [17] Hickerson, M. J. and Cunningham, C. W. Dramatic mitochondrial gene rearrangements in the hermit crab Pagurus longicarpus (Crustacea, Anomura). Molecular Biology and Evolution, 2000, 17: [18] Adams, M. D., Kelley, J. M., Gocayne, J. D. et al. Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 1991, 252:

6 [19] Stephenson, W. and Campbell, B.. The Australian portunids (Crustacea: Portunidae) IV. Remaining genera. Australian Journal of Marine and Freshwater Research, 1960, 11: [20] Estampador, E. P.. Studies on Scylla (Crustacea: Portunidae) I. Revision of the genus. Philippine Journal of Science, 1949, 78: [21] Keenan, C. P., Davie, P. J. F. and Mann, D. L. A revision of the genus Scylla de Haan, 1883 (Crustacea: Decapoda: Brachyuan: Portunidae). The Raffles Bulletin of Zoology, 1998, 46: [22] Bateman, A., Coin, L., Durbin, R., et al. The Pfam protein families database. Nucleic Acids Research, 2004, 32: D [23] Ma, L. B., Zhang, F. Y., Ma, C. Y., et al. Scylla paramamosain (Estampador) the most common mud crab (Genus Scylla) in China: evidence from mtdna. Aquaculture Research, 2006, 37: [24]. COI [J] [25] Gopurenko, D., Hughes, J. M. and Keenan, C. P. Mitochondrial DNA evidence for rapid colonisation of the Indo-West Pacific by the mud crab Scylla serrata. Marine Biology, 1999, 134: [26]. COI [J]., [27] Fratini, S. and Vannini, M. Genetic differentiation in the mud crab Scylla serrata (Decapoda: Portunidae) within the Indian Ocean. Journal of Experimental Marine Biology and Ecology, 2002, 272: [28] Masatsugu, T., Anna, B., Takuma, S., et al. Isolation and characterization of microsatellite DNA markers from mangrove crab, Scylla paramamosain. Molecular Ecology Notes, 2005, 5: [29] Xu, X. J., Wang, G. Z., Wang, K. J., et al. Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain. Conservation Genetics, 2009, 10: [30]. [J] [31]. [J] [32]. [J] [33]. [J] [34]. [J] [35]. DNA [J] [36] Imai, H., Obata, Y., Sekiya, S., et al. Mitochondrial DNA markers confirm successful stocking of mud crab juveniles, (Scylla paramamosain) into a natural population. Suisan Zoshoku, 2006, 50: [37]. [J] [38] Klinbunga, S., Boonyapakdee, A. and Pratoomchat, B. Genetic diversity and species-diagnostic markers of mud crabs (Genus Scylla) in eastern Thailand determined by RAPD analysis. Marine Biotechnology, 2000, 2: [39] Kitakado, T., Kitada, S., Obata, Y., et al. Simultaneous estimation of mixing rates and genetic drift under successive sampling of genetic markers with application to the mud crab (Scylla paramamosain) in Japan. Genetics, 2006, 173: [40]. Scylla [J] [41]. [J] [42]. [J] [43]. [J] [44]. 1 [J] [45]. DNA [J] [46]. [J] [47]. QTL [J] [48]. [J] [49]. Scophthalmus maximus [J]