In-vitro maturation of immature human oocytes

Size: px
Start display at page:

Download "In-vitro maturation of immature human oocytes"

Transcription

1 In-vitro maturation of immature human oocytes Focusing on the methodology Byron Asimakopoulos, PhD Associate Professor Faculty of Medicine, Democritus University of Thrace

2 In-vitro maturation The maturation in culture of immature oocytes after their recovery from follicles that may or may not have been exposed to exogenous gonadotropins. Prophase I Metaphase II Metaphase I Mataphase II

3 History of IVM 1935: Pincus & Enzmann reported that immature rabbit oocytes were capable of spontaneous maturation and fertilization in-vitro. [Pincus G, Enzmann EV (1935). J Exp Med, 62(5): ] 1965: Edwards RG. (1965a): Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature,208: Edwards RG (1965b): Maturation in vitro of human ovarian oocytes. Lancet, II: : Cha et al reported the first birth using IVM oocytes from an oocyte donor program [Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK (1991). Fertil Steril, 55: ] 1994: Trounson et al reported the first birth using IVM oocytes from an unstimulated PCO patient [Trounson A, Wood C, Kausche A (1994). Fertil Steril, 62(2): ]

4 Clinical applications of IVM Patients at risk of OHSS PCOS patients Patients with a history of OHSS Patients with limited time for controlled ovarian stimulation Patients who need initiation of gonadotoxic treatments Patients at risk of sustained elevations of E2 Patients with hormonally sensitive tumors

5 IVM in order to rescue immature oocytes retrieved in standard IVF cycles IVM in unstimulated or mildly stimulated cycles

6 The immature oocytes collected in standard IVF cycles are inherently abnormal They have failed to respond to ovulatory gonadotropin stimulus They show grossly aberrant gene expression (2766 genes) They have limited developmental competence after IVF/ICSI Jones GM, Cram DS, Song B, Magli MC, Gianaroli L, Lacham-Kaplan O, et al. Gene expression profiling of human oocytes following in vivo or in vitro maturation. Hum Reprod 2008;23: Nogueira D, Staessen C, Van de Velde H, Van Steirteghem A. Nuclear status and cytogenetics ofembryos derived from in vitro-matured oocytes. Fertil Steril 2000;74: Strassburger D, Goldstein A, Friedler S, Raziel A, Kasterstein E, Mashevich M, et al. The cytogenetic constitution of embryos derived from immature (metaphase I) oocytes obtained after ovarian hyperstimulation. Fertil Steril 2010;94:971 8.

7 Virant-Klun et al (2013). BioMed Research International

8 To prime or not to prime? No stimulation (natural cycle) FSH for 3-6 days, retrieval on day IU of hcg prior to retrieval Combination of FSH and hcg

9 The immature oocytes in the lab Culture in a conventional IVF medium? Culture in specific maturation media?

10 The maturation media have been designed for immature cumulus oocyte complexes Oocyte IVM media are intended for the culture of intact cumulus-oocyte complexes (COC). As COC contain cumulus cells, but just one oocyte, IVM media are designed principally to meet the nutritional needs of the somatic cells. Hence, IVM media are complex media capable of supporting the high metabolic requirements of the COC (e.g., very high glycolytic activity), whereas cleavage media are designed for the low metabolic needs of the early embryo (e.g., utilize glucose poorly) Gilchrist et al (2011). Fertil Steril, 96(2): e141

11 Sutton ML, Gilchrist RB, Thompson JG (2003). Hum Reprod Update, 9(1): 35-48

12 Factors promoting IVM Maturation Promoting Factor (MPF) Mitogen Activated Protein Kinase (MAPK) Oocyte size (diameter >115μ) FSH LH (few LHR in cumulus cells no (?) LHR in oocyte) Epidermal Growth Factor (EGF) Meiosis Activating Sterol (FF-MAS) Growth Hormone (GH) Insulin Insulin-like Growth Factor-I (IGF-I) Prolactin

13 The usual procedure Two types of media Washing and Preincubation of immature oocytes in the first medium Incubation for >28 hours in the second medium (maturation medium) The maturation medium is usually supplemented with FSH, hcg and serum proteins

14 Supplementation with FSH/LH SAGE IVM system

15 Supplementation with FSH/LH SAGE IVM system

16 IVM for how long?

17 Oocytes obtained after oophorectomy Gumala S, Kaufman ML, Racowsky C (1993). Assis Reprod Technol Androl, 4: 1-14

18

19 Common questions Maturation rate? Fertilization: Conventional IVF or ICSI? Embryo development: Do the IVM oocytes have the same developmental competence as the in-vivo matured oocytes? Implantation rate? Pregnancy rate?

20

21 Denuded oocytes

22 Preliminary results on the efficacy of a new IVM system N= 174 immature oocytes AB1a: GV oocytes for 4 hours AB1b: GV & MI oocytes for 46 hours Glucose, essential & non-essential aminoacids, sodium pyrouvate, sodium bicarbonate & gonadotropins maturation % GV MI GV MII MI MII

23 Álvarez et al (2013). Indian J Med Res, 137:

24 Strowitzki T (2013) Arch Gynecol Obstet, 288: Roesner et al (2012) Acta Obstet Gynecol Scand, 91: 22-27

25

26

27 Modified Tyrode s solution supplemented with human follicular fluid and amino acids

28

29 Vanhoutte et al (2005). Reprod Biol Endocrinol, 3(71). Denuded oocytes

30 Clinical outcome: IVF vs. IVM No randomized, controlled trials currently exist comparing IVF & IVM

31 Conclusions IVM is a useful method especially for patients at risk for OHSS, patients with PCOS or PCO-like ovaries, patients with estrogen sensitive tumors or patients with a limited time prior to gonadotoxin treatments Priming with HCG or/and FSH appears to improve pregnancy & implantation rates IVM with FSH/LH COCs Fertilization is possible either with conventional IVF or ICSI Pregnancy and implantation rates are lower than COH/IVF ~2500 children have been conceived with IVM, therefore the safety of IVM with regard to malformation and development outcomes can not be assessed accurately

Acupuncture Treatment For Infertile Women Undergoing Intracytoplasmic Sperm injection

Acupuncture Treatment For Infertile Women Undergoing Intracytoplasmic Sperm injection Acupuncture Treatment For Infertile Women Undergoing Intracytoplasmic Sperm injection Sandra L. Emmons, MD Phillip Patton, MD Source: Medical Acupuncture, A Journal For Physicians By Physicians Spring

More information

Welcome to chapter 8. The following chapter is called "Monitoring IVF Cycle & Oocyte Retrieval". The author is Professor Jie Qiao.

Welcome to chapter 8. The following chapter is called Monitoring IVF Cycle & Oocyte Retrieval. The author is Professor Jie Qiao. Welcome to chapter 8. The following chapter is called "Monitoring IVF Cycle & Oocyte Retrieval". The author is Professor Jie Qiao. The learning objectives of this chapter are 2 fold. The first section

More information

ANS 3319C Reproductive Physiology and Endocrinology Techniques for In-Vitro Embryo Production

ANS 3319C Reproductive Physiology and Endocrinology Techniques for In-Vitro Embryo Production ANS 3319C Reproductive Physiology and Endocrinology Techniques for In-Vitro Embryo Production Objectives 1) To gain an understanding of the process of in-vitro embryo production in cattle and other domestic

More information

AGE & FERTILITY: Effective Evaluation & Treatment I. LANE WONG, MD, FACOG. www.hopefertilitycenter.com www.hopeivf.com

AGE & FERTILITY: Effective Evaluation & Treatment I. LANE WONG, MD, FACOG. www.hopefertilitycenter.com www.hopeivf.com Page 1 of 6 AGE & FERTILITY: Effective Evaluation & Treatment I. LANE WONG, MD, FACOG. www.hopefertilitycenter.com www.hopeivf.com Age has a profound effect on female fertility. This is common knowledge,

More information

SPONTANEOUS AND PHARMACOLOGICALLY INDUCED REMISSIONS IN PATIENTS WITH PREMATURE OVARIAN FAILURE

SPONTANEOUS AND PHARMACOLOGICALLY INDUCED REMISSIONS IN PATIENTS WITH PREMATURE OVARIAN FAILURE SPONTANEOUS AND PHARMACOLOGICALLY INDUCED REMISSIONS IN PATIENTS WITH PREMATURE OVARIAN FAILURE David Kreiner, M. D. * Kathleen Droesch, M.D. Daniel Navot, M.D. Richard Scott, M.D. Zev Rosenwaks, M.D.

More information

ASSISTED REPRODUCTIVE TECHNOLOGIES (ART)

ASSISTED REPRODUCTIVE TECHNOLOGIES (ART) ASSISTED REPRODUCTIVE TECHNOLOGIES (ART) Dr. Herve Lucas, MD, PhD, Biologist, Andrologist Dr. Taher Elbarbary, MD Gynecologist-Obstetrician Definitions of Assisted Reproductive Technologies Techniques

More information

Systematic review by: Dr. Ashraf Ahmed ElDaly, M.Sc., M.D.

Systematic review by: Dr. Ashraf Ahmed ElDaly, M.Sc., M.D. Role of luteinizing hormone supplementation in the follicular phase and pregnancy, during controlled ovarian hyperstimulation in in vitro fertilization Systematic review by: Dr. Ashraf Ahmed ElDaly, M.Sc.,

More information

EFFECT OF INCREASED TESTOSTERONE LEVEL ON WOMAN S FERTILITY

EFFECT OF INCREASED TESTOSTERONE LEVEL ON WOMAN S FERTILITY 1 Nada Polyclinic, Po ega, Croatia 2 School of Medicine, University of Zagreb, Zagreb, Croatia Preliminary Communication Received: April 15, 2004 Accepted: June 16, 2004 EFFECT OF INCREASED TESTOSTERONE

More information

European IVF Monitoring (EIM) Year: 2010

European IVF Monitoring (EIM) Year: 2010 European IVF Monitoring (EIM) Year: 2010 Name of country: Poland Name and full address of contact person: Professor Rafal Kurzawa, MD PhD Fertility and Sterility Special Interest Group Polish Gynaecological

More information

Management fertility sparing degli endometriomi Errico Zupi

Management fertility sparing degli endometriomi Errico Zupi Management fertility sparing degli endometriomi Errico Zupi Università Tor Vergata Roma Management of endometrioma Pain Infertility Surgical treatment Medical treatment Infertility clinic Both medical

More information

Fast Track to IVF. Objectives

Fast Track to IVF. Objectives Disclosure statement: Richard H. Reindollar, M.D. has no relevant financial relationships with any manufacturers of pharmaceuticals, laboratory supplies, or medical devices. Fast Track to IVF Richard H.

More information

Effect of honeybee royal jelly on the nuclear maturation of bovine oocytes in vitro

Effect of honeybee royal jelly on the nuclear maturation of bovine oocytes in vitro CPh6.8 Effect of honeybee royal jelly on the nuclear maturation of bovine oocytes in vitro M Kuran 1*, E Sirin 1,E Soydan 1, AG Onal 2 Department of Animal Science, Universities of 1 Ondokuz Mayis, Samsun,

More information

The Menstrual Cycle. Model 1: Ovarian Cycle follicular cells

The Menstrual Cycle. Model 1: Ovarian Cycle follicular cells The Menstrual Cycle REVIEW questions to complete before starting this POGIL activity 1. Gonads produce both gametes and sex steroid hormones. For the female, name the: A. gonads ovaries B. gametes oocyte/ovum/egg

More information

In-vitro fertilization in a spontaneous cycle: easy, cheap and realistic

In-vitro fertilization in a spontaneous cycle: easy, cheap and realistic Human Reproduction vol.15 no.2 pp.314 318, 2000 In-vitro fertilization in a spontaneous cycle: easy, cheap and realistic R.M.J.Janssens 1, C.B.Lambalk, J.P.W.Vermeiden, a decrease in endometrial receptivity

More information

Fertility over 40. P.G. Bianchi

Fertility over 40. P.G. Bianchi Fertility over 40 P.G. Bianchi World records? The oldest woman to deliver has been Rosanna C. who delivered at 63 years and 3 months (oocyte egg donation program). The oldest women to carry a spontaneous

More information

Assisted Reproductive Technologies at IGO

Assisted Reproductive Technologies at IGO 9339 Genesee Avenue, Suite 220 San Diego, CA 92121 858 455 7520 Assisted Reproductive Technologies at IGO Although IGO no longer operates an IVF laboratory or program as such, we work closely with area

More information

Clinical Study Comparison of Endocrine Profile and In Vitro Fertilization Outcome in Patients with PCOS, Ovulatory PCO, or Normal Ovaries

Clinical Study Comparison of Endocrine Profile and In Vitro Fertilization Outcome in Patients with PCOS, Ovulatory PCO, or Normal Ovaries International Endocrinology Volume 2012, Article ID 492803, 6 pages doi:10.1155/2012/492803 Clinical Study Comparison of Endocrine Profile and In Vitro Fertilization Outcome in Patients with PCOS, Ovulatory

More information

The Outcome of Repeated In Vitro Fertilization-Embryo Transfer Based on the Endometrial Thickness

The Outcome of Repeated In Vitro Fertilization-Embryo Transfer Based on the Endometrial Thickness Bulletin of the Osaka Medical College 49 1, 2 5-9, 2003 5 Original Article The Outcome of Repeated In Vitro Fertilization-Embryo Transfer Based on the Endometrial Thickness Yoshiki YAMASHITA, Toshimitsu

More information

The objectives of this chapter are: To provide an understanding of the various stimulation protocols used in IVF To enable the student to understand

The objectives of this chapter are: To provide an understanding of the various stimulation protocols used in IVF To enable the student to understand 1 The objectives of this chapter are: To provide an understanding of the various stimulation protocols used in IVF To enable the student to understand the factors affecting the choice of protocol based

More information

Nordic Fertility Society. Quality Guide. Checklist for ART Clinic and ART laboratory

Nordic Fertility Society. Quality Guide. Checklist for ART Clinic and ART laboratory Nordic Fertility Society Quality Guide Checklist for ART Clinic and ART laboratory Yes, not-applicable, No CLINICAL CHECK LIST PATIENT INFORMATION Is there a printed patient information on: Ovarian Stimulation?

More information

Recent Progress in In Vitro Fertilization and Intracytoplasmic Sperm Injection Technologies in Japan

Recent Progress in In Vitro Fertilization and Intracytoplasmic Sperm Injection Technologies in Japan Research and Reviews Recent Progress in In Vitro Fertilization and Intracytoplasmic Sperm Injection Technologies in Japan JMAJ 52(1): 29 33, 2009 Kaoru YANAGIDA* 1 Abstract The three basic pillars of fertility

More information

Spindle imaging: a new marker for optimal timing of ICSI?

Spindle imaging: a new marker for optimal timing of ICSI? Human Reproduction Vol.19, No.3 pp. 649±654, 2004 Advance Access publication 29 January, 2004 DOI: 10.1093/humrep/deh113 Spindle imaging: a new marker for optimal timing of ICSI? Y.Cohen, M.Malcov, T.Schwartz,

More information

Rationale for replacing IVIG with Intralipid (IL) for immunological pregnancy loss

Rationale for replacing IVIG with Intralipid (IL) for immunological pregnancy loss Rationale for replacing IVIG with Intralipid (IL) for immunological pregnancy loss Recurrent Pregnancy Loss The reason that an embryo may not implant successfully is either because there is something intrinsically

More information

Symposium on RECENT ADVANCES IN ASSISTED REPRODUCTIVE TECHNOLOGY

Symposium on RECENT ADVANCES IN ASSISTED REPRODUCTIVE TECHNOLOGY Symposium on RECENT ADVANCES IN ASSISTED REPRODUCTIVE TECHNOLOGY Dr Niel Senewirathne Senior Consultant of Obstetrician & Gynaecologist De zoyza Maternity Hospita 1 ART - IVF & ICSI 2 Infertility No pregnancy

More information

Elevated serum progesterone on the day of HCG administration in IVF is associated with a higher pregnancy rate in polycystic ovary syndrome

Elevated serum progesterone on the day of HCG administration in IVF is associated with a higher pregnancy rate in polycystic ovary syndrome Human Reproduction vol.14 no.3 pp.601 605, 1999 Elevated serum progesterone on the day of HCG administration in IVF is associated with a higher pregnancy rate in polycystic ovary syndrome Nicola Doldi

More information

Endocrinology of the Female Reproductive Axis

Endocrinology of the Female Reproductive Axis Endocrinology of the Female Reproductive Axis girlontheriver.com Geralyn Lambert-Messerlian, PhD, FACB Professor Women and Infants Hospital Alpert Medical School at Brown University Women & Infants BROWN

More information

1. AMOUNT OF FSH PRESENT

1. AMOUNT OF FSH PRESENT The Menstrual Cycle Name Date Period PRE-LAB 1. Write down three facts you know about the menstrual cycle. A. B. C. FOLLICULAR PHASE Within the ovaries are located many egg cells. Each egg is enclosed

More information

European IVF Monitoring (EIM) Year: 2008

European IVF Monitoring (EIM) Year: 2008 European IVF Monitoring (EIM) Year: 2008 Name of country POLAND Name and full address of contact person. professor Rafal Kurzawa MD PhD Fertility and Sterility Special Interest Group Polish Gynaecological

More information

Research Article Association of ABO Blood Type and Ovarian Stimulation Response in Oocyte Donors

Research Article Association of ABO Blood Type and Ovarian Stimulation Response in Oocyte Donors Cronicon OPEN ACCESS Nigel Pereira 1 *, Anne P Hutchinson 2, Jovana P Lekovich 1, Rony T Elias 1, Zev Rosenwaks 1 and Steven D Spandorfer 1 1 Ronald O Perelman and Claudia Cohen Center for Reproductive

More information

Drug Therapy Guidelines: Injectable Fertility Medications

Drug Therapy Guidelines: Injectable Fertility Medications Drug Therapy Guidelines: Injectable Fertility Medications Effective Date: 11/20/07 Committee Review Date: 7/12/00, 5/8/01, 1/15/02, 5/6/0, 12/16/0, 6/8/04, 12/16/05, 2/1/06, 10/15/06, 7/20/07, 11/5/07

More information

Original Article Comparison of different ovarian hyperstimulation protocols efficacy in poor ovarian responders according to the Bologna criteria

Original Article Comparison of different ovarian hyperstimulation protocols efficacy in poor ovarian responders according to the Bologna criteria Int J Clin Exp Med 2014;7(4):1128-1134 www.ijcem.com /ISSN:1940-5901/IJCEM1402034 Original Article Comparison of different ovarian hyperstimulation protocols efficacy in poor ovarian responders according

More information

East and North Hertfordshire CCG Fertility treatment and referral criteria for tertiary level assisted conception. December 2014

East and North Hertfordshire CCG Fertility treatment and referral criteria for tertiary level assisted conception. December 2014 East and North Hertfordshire CCG Fertility treatment and referral criteria for tertiary level assisted conception December 2014 1 1. Introduction This policy sets out the entitlement and service that will

More information

Infertility Services Medical Policy For University of Vermont Medical Center and Central Vermont Medical Center employer groups

Infertility Services Medical Policy For University of Vermont Medical Center and Central Vermont Medical Center employer groups Infertility Services Medical Policy For University of Vermont Medical Center and Central Vermont Medical Center employer groups File name: Infertility Services File code: UM.REPRO.01 Last Review: 02/2016

More information

The cost-effectiveness of IVF in the UK: a comparison of three gonadotrophin treatments Sykes D, Out H J, Palmer S J, van Loon J

The cost-effectiveness of IVF in the UK: a comparison of three gonadotrophin treatments Sykes D, Out H J, Palmer S J, van Loon J The cost-effectiveness of IVF in the UK: a comparison of three gonadotrophin treatments Sykes D, Out H J, Palmer S J, van Loon J Record Status This is a critical abstract of an economic evaluation that

More information

INFERTILITY/POLYCYSTIC OVARIAN SYNDROME. Ovulatory Dysfunction: Polycystic ovarian syndrome (PCOS)

INFERTILITY/POLYCYSTIC OVARIAN SYNDROME. Ovulatory Dysfunction: Polycystic ovarian syndrome (PCOS) Introduction Infertility is defined as the absence of pregnancy following 12 months of unprotected intercourse. Infertility may be caused by Ovulatory Dysfunction, Blocked Fallopian Tubes, Male Factor

More information

Lecture 2 Conception and Pregnancy Loss

Lecture 2 Conception and Pregnancy Loss This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike License. Your use of this material constitutes acceptance of that license and the conditions of use of materials on this

More information

Cost-effective approaches to in vitro fertilization: Means to improve access

Cost-effective approaches to in vitro fertilization: Means to improve access International Journal of Gynecology and Obstetrics (2006) 94, 287 291 www.elsevier.com/locate/ijgo FULFILLING WOMEN S REPRODUCTIVE INTENTIONS Cost-effective approaches to in vitro fertilization: Means

More information

Clinical Policy Committee

Clinical Policy Committee Northern, Eastern and Western Devon Clinical Commissioning Group South Devon and Torbay Clinical Commissioning Group Clinical Policy Committee Commissioning policy: Assisted Conception Fertility assessment

More information

Artificial insemination with donor sperm

Artificial insemination with donor sperm Artificial insemination with donor sperm Ref. 123 / 2009 Reproductive Medicine Unit Servicio de Medicina de la Reproducción Gran Vía Carlos III 71-75 08028 Barcelona Tel. (+34) 93 227 47 00 Fax. (+34)

More information

In vitro fertilization for Orthodox Jewish couples: antagonist cycle modifications allowing for mikveh attendance before oocyte retrieval

In vitro fertilization for Orthodox Jewish couples: antagonist cycle modifications allowing for mikveh attendance before oocyte retrieval In vitro fertilization for Orthodox Jewish couples: antagonist cycle modifications allowing for mikveh attendance before oocyte retrieval David E. Reichman, M.D., Anate Aelion Brauer, M.D., Dan Goldschlag,

More information

In - Vitro Fertilization Handbook

In - Vitro Fertilization Handbook In - Vitro Fertilization Handbook William F. Ziegler, D.O. Medical Director Scott Kratka, ELD, TS Embryology Laboratory Director Lauren F. Lucas, P.A.-C, M.S. Physician Assistant Frances Cerniak, R.N.

More information

Ehlers-Danlos Syndrome Fertility Issues. Objectives

Ehlers-Danlos Syndrome Fertility Issues. Objectives Ehlers-Danlos Syndrome Fertility Issues Baltimore Inner Harbor Independence Day Brad Hurst, M.D. Professor Reproductive Endocrinology Carolinas Medical Center - Charlotte, North Carolina Objectives Determine

More information

MODEL FORM. [Program s SART Name and Number] INFORMED CONSENT FOR EGG DONORS

MODEL FORM. [Program s SART Name and Number] INFORMED CONSENT FOR EGG DONORS MODEL FORM [Program s SART Name and Number] INFORMED CONSENT FOR EGG DONORS You are agreeing to undergo a cycle of egg donation at [program s SART name]. Do not sign this document if you have not received

More information

Standard Operating Procedure IVF and ICSI procedures

Standard Operating Procedure IVF and ICSI procedures Pages: 1 of 6 PRINCIPLES Oocytes are aspirated from mature Graafian follicles after the administration of ovarian hyperstimulation pharmacological regimes designed to produce multiple follicular development.

More information

IN VITRO FERTILISATION IVF and ICSI

IN VITRO FERTILISATION IVF and ICSI IN VITRO FERTILISATION IVF and ICSI Page 1 of 7 WHAT ARE IVF and ICSI? IVF is short for in vitro fertilisation which means fertilisation outside the body. It usually involves stimulation of the ovaries

More information

PREVENTION OF INFERTILITY SOURCE DOCUMENT THE IMPACT OF AGE ON FEMALE FERTILITY

PREVENTION OF INFERTILITY SOURCE DOCUMENT THE IMPACT OF AGE ON FEMALE FERTILITY PREVENTION OF INFERTILITY SOURCE DOCUMENT THE IMPACT OF AGE ON FEMALE FERTILITY BACKGROUND Over the past several decades, demographic and socioeconomic trends have resulted in an increase in the absolute

More information

Preimplantation Genetic Diagnosis (PGD) in Western Australia

Preimplantation Genetic Diagnosis (PGD) in Western Australia Preimplantation Genetic Diagnosis (PGD) in Western Australia Human somatic cells have 46 chromosomes each, made up of the 23 chromosomes provided by the egg and the sperm cell from each parent. Each chromosome

More information

Intracytoplasmic sperm injection in the horse: the ultimate blind date

Intracytoplasmic sperm injection in the horse: the ultimate blind date Acta Scientiae Veterinariae. 38(Supl 2): s559-s564, 2010. ISSN 1678-0345 (Print) ISSN 1679-9216 (Online) Intracytoplasmic sperm injection in the horse: the ultimate blind date L.F. Campos-Chillon 1, T.K.

More information

Informed Consent Packet - In Vitro Fertilization (IVF)

Informed Consent Packet - In Vitro Fertilization (IVF) Center for Reproductive Medicine (CRM) Informed Consent Packet - In Vitro Fertilization (IVF) This packet contains the required IVF treatment consent documents. Please read, consider and, if you agree,

More information

A POWERFUL IN VITRO FERTILIZATION

A POWERFUL IN VITRO FERTILIZATION A POWERFUL During the past 50 years technological advances in the field of bovine reproduction have led to some dramatic changes in the way cattle look, reproduce, perform, and even taste. Artificial Insemination

More information

In Vitro Fertilization (IVF) Page 1 of 11

In Vitro Fertilization (IVF) Page 1 of 11 In Vitro Fertilization (IVF) Page 1 of 11 This document is a part of your informed consent process. Both partners should read the entire document carefully. In vitro fertilization (IVF) is a treatment

More information

Metformin treatment before IVF / ICSI in non obese PCOS women

Metformin treatment before IVF / ICSI in non obese PCOS women Metformin treatment before IVF / ICSI in non obese PCOS women A Nordic, prospective, randomised, double blind, multicentre study Sigrun Kjøtrød University Hospital of Trondheim, Norway Metformin effects

More information

Clinical Relevance of Combined FSH and AMH Observations in Infertile Women

Clinical Relevance of Combined FSH and AMH Observations in Infertile Women ORIGINAL Endocrine ARTICLE Research Clinical Relevance of Combined FSH and AMH Observations in Infertile Women Norbert Gleicher, Ann Kim, Vitaly Kushnir, Andrea Weghofer, Aya Shohat-Tal, Emanuela Lazzaroni,

More information

Reproduction and its Hormonal Control

Reproduction and its Hormonal Control Reproduction and its Hormonal Control Page 1 Reproduction and its Hormonal Control Different mammals have different patterns of reproduction Eg mammals, rats and mice can breed all year round, whereas

More information

Case Report J Med Cases 2013;4(4):221-226. Juan Carlos Castillo a, c, Joaquin Moreno a, Miguel Dolz a, Fernando Bonilla-Musoles b.

Case Report J Med Cases 2013;4(4):221-226. Juan Carlos Castillo a, c, Joaquin Moreno a, Miguel Dolz a, Fernando Bonilla-Musoles b. Elmer Press Case Report Successful Pregnancy Following Dual Triggering Concept (rhcg + GnRH Agonist) in a Patient Showing Repetitive Inmature Oocytes and Empty Follicle Syndrome: Case Report Juan Carlos

More information

SO, WHAT IS A POOR RESPONDER?

SO, WHAT IS A POOR RESPONDER? SO, WHAT IS A POOR RESPONDER? We now understand why ovarian reserve is important and how we assess it, but how is poor response defined? Unfortunately, there is no universally accepted definition for the

More information

Welcome to chapter 2. The following chapter is called "Indications For IVF". The author is Dr Kamini A. Rao.

Welcome to chapter 2. The following chapter is called Indications For IVF. The author is Dr Kamini A. Rao. Welcome to chapter 2. The following chapter is called "Indications For IVF". The author is Dr Kamini A. Rao. The indications for an IVF treatment have increased since the birth of the first IVF baby. The

More information

Consultations & other investigations

Consultations & other investigations Pricelist Please note as part of pre-treatment consultations, you may be required to have blood tests performed, the costs of which are not included in the treatment cycles. These costs are outlined below.

More information

How To Get A Refund On An Ivf Cycle

How To Get A Refund On An Ivf Cycle 100% IVF Refund Program Community Hospital North Clearvista Dr. N Dr. David Carnovale and Dr. Jeffrey Boldt, along with everyone at Community Reproductive Endocrinology, are committed to providing you

More information

IVF. IVF: The Success of Technology. Recent Advances in Assisted Reproductive Technologies 10/20/2007

IVF. IVF: The Success of Technology. Recent Advances in Assisted Reproductive Technologies 10/20/2007 Obstetrics and Gynecology Update: What Does the Evidence Tell Us? 1 In Vitro Fertilization () 2 Recent Advances in Assisted Reproductive Technologies Shehua Shen, M.D., ELD (ABB) Assistant Professor Department

More information

Headquarters in Sioux Center, IA 1

Headquarters in Sioux Center, IA 1 Nicholas Lemmel Cornfields, soybeans, and cows, I had finally arrived at Trans Ova Genetics Headquarters in Sioux Center, Iowa. I pulled into the drive of the intern house located on the corner of the

More information

Minimum standards for ICSI use, screening, patient information and follow-up in WA fertility clinics. January 2006

Minimum standards for ICSI use, screening, patient information and follow-up in WA fertility clinics. January 2006 Minimum standards for ICSI use, screening, patient information and follow-up in WA fertility clinics January 2006 1. BACKGROUND ICSI has been shown to be effective for male factor infertility and it also

More information

THE CENTER FOR ADVANCED REPRODUCTIVE SERVICES (CARS) (The Center) CONSENT FOR IN VITRO FERTILIZATION AND EMBRYO TRANSFER

THE CENTER FOR ADVANCED REPRODUCTIVE SERVICES (CARS) (The Center) CONSENT FOR IN VITRO FERTILIZATION AND EMBRYO TRANSFER THE CENTER FOR ADVANCED REPRODUCTIVE SERVICES (CARS) (The Center) CONSENT FOR IN VITRO FERTILIZATION AND EMBRYO TRANSFER Partner #1 Last Name (Surname): Partner #1 First Name: Partner #1 Last 5 Digits

More information

Emmett F. Branigan, MD,* Antoinette Estes, BS, Kenneth Walker, BS, Jillian Rothgeb, BS. Bellingham IVF and Infertility Center, Bellingham, WA

Emmett F. Branigan, MD,* Antoinette Estes, BS, Kenneth Walker, BS, Jillian Rothgeb, BS. Bellingham IVF and Infertility Center, Bellingham, WA American Journal of Obstetrics and Gynecology (2006) 194, 1696 701 www.ajog.org Thorough sonographic oocyte retrieval during in vitro fertilization produces results similar to ovarian wedge resection in

More information

Weighing the impact of obesity on female reproductive function and fertility

Weighing the impact of obesity on female reproductive function and fertility bs_bs_banner Supplement Article Weighing the impact of obesity on female reproductive function and fertility Emily S Jungheim, Jennifer L Travieso, and Margaret M Hopeman Obesity in women is associated

More information

From Menses to Menopause: How Hormones Can Affect Blood Glucose Levels. Christine Day, RN, MS, CNS-BC Lake Superior College

From Menses to Menopause: How Hormones Can Affect Blood Glucose Levels. Christine Day, RN, MS, CNS-BC Lake Superior College From Menses to Menopause: How Hormones Can Affect Blood Glucose Levels Christine Day, RN, MS, CNS-BC Lake Superior College Overview Will review hormonal changes over the female lifespan Discuss the effects

More information

Human in vitro fertilization

Human in vitro fertilization Advanced Information Human in vitro fertilization The 2010 Nobel Prize in Physiology or Medicine is awarded to Dr. Robert G. Edwards for the development of human in vitro fertilization (IVF), a medical

More information

Day -1 RETRIEVAL OF OVARIES AND OOCYTE COLLECTION AND MATURATION

Day -1 RETRIEVAL OF OVARIES AND OOCYTE COLLECTION AND MATURATION Day -1 RETRIEVAL OF OVARIES AND OOCYTE COLLECTION AND MATURATION OVARY COLLECTION Materials and Equipment Needed Thermos with 2 containers with 0.5 L of transport saline in each. Appropriate attire as

More information

Effect of pretreatment with transdermal testosterone on poor ovarian responders undergoing IVF/ICSI: A meta-analysis

Effect of pretreatment with transdermal testosterone on poor ovarian responders undergoing IVF/ICSI: A meta-analysis EXPERIMENTAL AND THERAPEUTIC MEDICINE 8: 187-194, 2014 Effect of pretreatment with transdermal testosterone on poor ovarian responders undergoing IVF/ICSI: A meta-analysis SHAN LUO, SHANGWEI LI, XIAOHONG

More information

Biochimica et Biophysica Acta

Biochimica et Biophysica Acta Biochimica et Biophysica Acta 1810 (2011) 843 852 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journal homepage: www.elsevier.com/locate/bbagen In vitro fertilization: Four decades

More information

INFORMED CONSENT AND AUTHORIZATION FOR IN VITRO FERTILIZATION OF PREVIOUSLY CRYOPRESERVED OOCYTES

INFORMED CONSENT AND AUTHORIZATION FOR IN VITRO FERTILIZATION OF PREVIOUSLY CRYOPRESERVED OOCYTES INFORMED CONSENT AND AUTHORIZATION FOR IN VITRO FERTILIZATION OF PREVIOUSLY CRYOPRESERVED OOCYTES We, the undersigned, as patient and partner understand that we will be undergoing one or more procedures

More information

Understanding Blood Tests - Pregnancy/Fertility Monitoring by Beth Anne Ary M.D

Understanding Blood Tests - Pregnancy/Fertility Monitoring by Beth Anne Ary M.D Understanding Blood Tests - Pregnancy/Fertility Monitoring by Beth Anne Ary M.D Blood tests are the most common and most important method of monitoring pregnancy-- both assisted pregnancies, and unassisted.

More information

Reduced Ovarian Reserve Is there any hope for a bad egg?

Reduced Ovarian Reserve Is there any hope for a bad egg? Reduced Ovarian Reserve Is there any hope for a bad egg? Dr. Phil Boyle Galway Clinic, 19 th March 2014 For more information on Low AMH see www.napro.ie Anti Mullerian Hormone AMH levels are commonly measured

More information

How do fertility drugs work?

How do fertility drugs work? How do fertility drugs work? Under normal circumstances, ovulation occurs once a month when a ripened egg which is ready to be fertilised is released from the ovaries. For couples who are trying to conceive,

More information

Assisted reproductive technology in Australia and New Zealand 2010

Assisted reproductive technology in Australia and New Zealand 2010 Assisted reproductive technology in Australia and New Zealand 2010 ASSISTED REPRODUCTION SERIES Number 16 Assisted reproductive technology in Australia and New Zealand 2010 Alan Macaldowie Yueping A Wang

More information

Article. Laura Detti, MD, Frank D. Yelian, MD, PhD, Michael L. Kruger, MA, Michael P. Diamond, MD, Elizabeth E. Puscheck, MD

Article. Laura Detti, MD, Frank D. Yelian, MD, PhD, Michael L. Kruger, MA, Michael P. Diamond, MD, Elizabeth E. Puscheck, MD Article Endometrial Thickness Dynamics and Morphologic Characteristics During Pituitary Downregulation With Antagonists in Assisted Reproductive Technology Cycles Laura Detti, MD, Frank D. Yelian, MD,

More information

REPRODUCTIVE MEDICINE AND INFERTILITY ASSOCIATES Woodbury Medical Arts Building 2101 Woodwinds Drive Woodbury, MN 55125 (651) 222-6050

REPRODUCTIVE MEDICINE AND INFERTILITY ASSOCIATES Woodbury Medical Arts Building 2101 Woodwinds Drive Woodbury, MN 55125 (651) 222-6050 REPRODUCTIVE MEDICINE AND INFERTILITY ASSOCIATES Woodbury Medical Arts Building 2101 Woodwinds Drive Woodbury, MN 55125 (651) 222-6050 RECIPIENT COUPLE INFORMED CONSENT AND AUTHORIZATION FOR IN VITRO FERTILIZATION

More information

Preimplantation Genetic Diagnosis (PGD) for Fanconi Anemia and HLA matching

Preimplantation Genetic Diagnosis (PGD) for Fanconi Anemia and HLA matching Preimplantation Genetic Diagnosis (PGD) for Fanconi Anemia and HLA matching Andria G. Besser, BEd, MS, CGC Licensed Genetic Counselor Reproductive Genetics Institute Chicago, IL Outline PGD overview In

More information

Assisted reproductive technologies (ART) in Canada: 2011 results from the Canadian ART Register

Assisted reproductive technologies (ART) in Canada: 2011 results from the Canadian ART Register 1 Assisted reproductive technologies (ART) in Canada: 2011 results from the Canadian ART Register Joanne Gunby, M.Sc. CARTR Co-ordinator Email: gunbyj@mcmaster.ca Supported by the IVF Directors Group of

More information

Fertility Preservation in Women with Cancer. Objectives. Patient #1 10/24/2011. The audience will understand: How cancer therapy affects fertility.

Fertility Preservation in Women with Cancer. Objectives. Patient #1 10/24/2011. The audience will understand: How cancer therapy affects fertility. Fertility Preservation in Women with Cancer Leslie R. DeMars Dartmouth-Hitchcock Medical Center Objectives The audience will understand: How cancer therapy affects fertility. Who should be considered for

More information

CLINICAL PHARMACOLOGY

CLINICAL PHARMACOLOGY FOR SUBCUTANEOUS USE ONLY DESCRIPTION Ganirelix Acetate Injection is a synthetic decapeptide with high antagonistic activity against naturally occurring gonadotropin-releasing hormone (GnRH). Ganirelix

More information

Endometriosis, Fertility and Pregnancy

Endometriosis, Fertility and Pregnancy This leaflet covers endometriosis and fertility. It provides information for women who have been diagnosed with endometriosis who would like to know if and how this can affect their fertility, and for

More information

Medical Policy Preimplantation Genetic Testing

Medical Policy Preimplantation Genetic Testing Medical Policy Preimplantation Genetic Testing Table of Contents Policy: Commercial Coding Information Information Pertaining to All Policies Policy: Medicare Description References Authorization Information

More information

Clinical Reference Group Quality & Safety Committee Governing Body. Policy Screened

Clinical Reference Group Quality & Safety Committee Governing Body. Policy Screened Fertility Policy 1 SUMMARY This policy is intended to support individuals and couples who want to become parents but who have a possible pathological problem (physical or psychological) leading to them

More information

Fertility care for women diagnosed with cancer

Fertility care for women diagnosed with cancer Saint Mary s Hospital Department of Reproductive Medicine Fertility care for women diagnosed with cancer Information For Patients INF/DRM/NUR/16 V1/01/11/2013 1 2 Contents Page Overview 4 Our Service 4

More information

CONSENT TO PARTICIPATE IN THE IN VITRO FERTILIZATION-EMBRYO TRANSFER PROGRAM

CONSENT TO PARTICIPATE IN THE IN VITRO FERTILIZATION-EMBRYO TRANSFER PROGRAM CONSENT TO PARTICIPATE IN THE IN VITRO FERTILIZATION-EMBRYO TRANSFER PROGRAM I, after consultation with my physician, request to participate in the In Vitro Fertilization (IVF)-Embryo Transfer (ET) procedures

More information

NON MEDICARE FEES CANBERRA FERTILITY CENTRE VERSION JANUARY 2015 AM QWB 295

NON MEDICARE FEES CANBERRA FERTILITY CENTRE VERSION JANUARY 2015 AM QWB 295 NON MEDICARE FEES CANBERRA FERTILITY CENTRE VERSION JANUARY 2015 AM QWB 295 1 CANBERRA FERTILITY CENTRE Suite 9, Level 2, Peter Yorke Building, Calvary John James Hospital, 173 Strickland Crescent, DEAKIN

More information

Midland Fertility Services

Midland Fertility Services Midland Fertility Services ICSI: Intra Cytoplasmic Sperm Injection Building futures, transforming lives Useful Contacts Infertility Network UK 0870 118 8088 www.infertilitynetworkuk.com British Infertility

More information

Commissioning Policy for In Vitro Fertilisation (IVF)/ Intracytoplasmic Sperm Injection (ICSI) within tertiary Infertility Services.

Commissioning Policy for In Vitro Fertilisation (IVF)/ Intracytoplasmic Sperm Injection (ICSI) within tertiary Infertility Services. East Midlands CCGs Commissioning Policy for In Vitro Fertilisation (IVF)/ Intracytoplasmic Sperm Injection (ICSI) within tertiary Infertility Services April 2014 CONTENTS Page 1. INTRODUCTION 3 2. GENERAL

More information

The following chapter is called "Follow-ups with a Positive or a Negative Pregnancy Test".

The following chapter is called Follow-ups with a Positive or a Negative Pregnancy Test. Slide 1 Welcome to chapter 7. The following chapter is called "Follow-ups with a Positive or a Negative Pregnancy Test". The author is Professor Pasquale Patrizio. Slide 2 This chapter has the following

More information

FERTILITY AND AGE. Introduction. Fertility in the later 30's and 40's. Am I fertile?

FERTILITY AND AGE. Introduction. Fertility in the later 30's and 40's. Am I fertile? FERTILITY AND AGE Introduction Delaying pregnancy is a common choice for women in today's society. The number of women in their late 30s and 40s attempting pregnancy and having babies has increased in

More information

COVENTRY HEALTH CARE OF ILLINOIS, INC. COVENTRY HEALTH CARE OF MISSOURI, INC. Medical Management Policy and Procedure PROPRIETARY

COVENTRY HEALTH CARE OF ILLINOIS, INC. COVENTRY HEALTH CARE OF MISSOURI, INC. Medical Management Policy and Procedure PROPRIETARY COVENTRY HEALTH CARE OF ILLINOIS, INC. COVENTRY HEALTH CARE OF MISSOURI, INC. Medical Management Policy and Procedure PROPRIETARY Policy: Infertility Evaluation and Treatment Number: MM 1306 Date Effective:

More information

Martha Luna, M.D., a,b Lawrence Grunfeld, M.D., a,b Tanmoy Mukherjee, M.D., a,b Benjamin Sandler, M.D., a,b and Alan Barry Copperman, M.D.

Martha Luna, M.D., a,b Lawrence Grunfeld, M.D., a,b Tanmoy Mukherjee, M.D., a,b Benjamin Sandler, M.D., a,b and Alan Barry Copperman, M.D. ARTICLE IN PRESS Moderately elevated levels of basal follicle-stimulating hormone in young patients predict low ovarian response, but should not be used to disqualify patients from attempting in vitro

More information

Consent for In Vitro Fertilization

Consent for In Vitro Fertilization Consent for In Vitro Fertilization Print Patient s Name Print Partner s Name We (I), the undersigned, request, authorize and consent to the procedure of In Vitro Fertilization (IVF) and Embryo Transfer

More information

50% Off Cycle 3 $ 9,900 $ 8,700 $ 7,500

50% Off Cycle 3 $ 9,900 $ 8,700 $ 7,500 Specialists In Reproductive Medicine & Surgery, P.A. www.dreamababy.com Fertility@DreamABaby.com Excellence, Experience & Ethics In Vitro Fertilization Price List (2015) We here at Specialists in Reproductive

More information

Preimplantation genetic diagnosis new method of screening of 24 chromosomes with the Array CGH method...2

Preimplantation genetic diagnosis new method of screening of 24 chromosomes with the Array CGH method...2 August 2012 content 8 Preimplantation genetic diagnosis new method of screening of 24 chromosomes with the Array CGH method...2 Maintaining fertility new opportunities in GENNET...3 Hysteroscopy without

More information

Tower Hamlets CCG Fertility policy

Tower Hamlets CCG Fertility policy Tower Hamlets CCG Fertility policy Approved December 2014 Introduction Tower Hamlets CCG is responsible for commissioning a range of health services including hospital, mental health and community services

More information

ARTICLE IN PRESS CONTROVERSY: ACUPUNCTURE

ARTICLE IN PRESS CONTROVERSY: ACUPUNCTURE CONTROVERSY: ACUPUNCTURE Acupuncture on the day of embryo transfer significantly improves the reproductive outcome in infertile women: a prospective, randomized trial Lars G. Westergaard, M.D., Ph.D.,

More information

Topic: Male Factor Infertility

Topic: Male Factor Infertility Topic: Male Factor Infertility Topic Overview: Male Factor Infertility Comparisons of pregnancy rates at insemination based on total motile sperm counts from the 1999 and 21 World Health Organization (WHO)

More information

Project proposal. Reproductive tourism in India: A description of surrogate mothers and their offspring. Medical student Malene Tanderup Kristensen

Project proposal. Reproductive tourism in India: A description of surrogate mothers and their offspring. Medical student Malene Tanderup Kristensen Project proposal Reproductive tourism in India: A description of surrogate mothers and their offspring Background Medical student Malene Tanderup Kristensen Traditional surrogacy is defined as where the

More information