Effects of Mo fertilization on growth nutrient and total saponin contents of Pairs polyphylla var. yunnanensis

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1 Plant Nutrition and Fertilizer Science * mg /kg mg /kg mg /kg 0. 8 mg /kg Mo 0. 8 mg /kg Mo 0. 2 ~ 0. 4 mg /kg S R A X Effects of Mo fertilization on growth nutrient and total saponin contents of Pairs polyphylla var. yunnanensis HE Zhong-jun 1 2 LIANG She-wang 3 ZENG Bo 4 GUAN Kai-yun 5* XIONG Jun-fen 1 1 College of Resource and Environmental Science Yunnan Agricultural University Kunming China 2 Post-doctoral Fellowship Station of Yunnan Baiyao Group Co. Ltd Kunming Botany Institute of Chinese Academy of Sciences Kunming China 3 College of Agronomy and Bio-technology Yunnan Agricultural University Kunming China 4 Agricultural Bureau of Yueyang City Yueyang Hunan China 5 Kunming Botany Institute of Chinese Academy of Sciences Kunming China Abstract A pot experiment was carried out to study the effects of Mo application on growth biomass nutrient contents in different parts and total saponin content in rhizome of Paris polyphylla var. yunnanenis. There were four levels of Mo application and 0. 8 mg /kg. The results show that the plant height photosynthesis rate biomass and rhizome are increased significantly with the application of Mo the highest values are and 0. 2 Mo mg /kg respectively. There is an increasing tendency of N contents in leaves and new rhizome under the medium and low levels of Mo mg /kg compared with no Mo treatment control while the N contents in leaves and new rhizome are significantly decreased under the high level of Mo 0. 8 mg /kg. The P contents in leaves and new rhizome are decreased under the Mo applications while the P contents in stems are increased. The K contents in leaves are increased under the Mo applications while the K contents in stems are decreased. The Mo contents in leaves stems and new rhizome of the Mo application treatments are significantly higher than those of the control and the tendency is increased at first and then decreased. The total saponin contents in new rhizome of the Mo application treatments are significantly increased compared with control the highest of which is the Mo Tel hezhongjun@ hotmail. com * Tel guanky@ kib. ac. cn

2 mg /kg treatment. Comprehensively considering the growth status increased weight and saponin content of rhizome Mo contents in different parts the suitable level of Mo application is between 0. 2 and 0. 4 mg /kg for Paris polyphylla var. yunnanenis in this experiment. Key words Paris polyphylla var. yunnanenis Mo growth status nutrient content total saponin content Paris L. 60% ~ 3100 m cm ph g /kg g /kg g /kg g /kg mg /kg mg /kg mg /kg Mo mg /kg mg /kg 4 21 cm 23 cm 5 kg 1 N P 2 O 5 K 2 O 150 mg /kg 7-8 CO NH 2 2 NaH 2 PO 4 2H 2 O K 2 SO Na 2 MoO H 2 O ~ 1. 0 mg /kg 60% 100 mg /kg kg 5% ~ 17. 5% 1 mm mm 17 ph = 1 5 ph 0. 5 mol /L NaHCO ± 10 g 0 20 cm 1 mol /L NH 4 OA C NaOH NaOH -

3 % - Excel SPSS 18 H 2 SO 4 -H 2 O H 2 SO 4 -H 2 O LI Mo 0. 4 Mo 0. 8 mg /kg 7 ~ Mo 0. 4 mg /kg mm 1 g 10 ml 30 min % % Aglient % Mo 0. 4 mg /kg / 65 / nm 0. 2 ~ 0. 4 mg /kg 0. 8 mg /kg Mo 0. 2 mg /kg = - / Table 1 1 Growth and biomass of different parts of Paris polyphilla var. yunnanensis under different Mo levels Mo level mg /kg Height cm Photos. rate CO 2 mmol / m 2 s Fresh weight g /pot Rhizome Shoot Perc. of increase % Biomass Rhizome Mo c b a b b b Mo bc a b b ab a Mo a a ab a a ab Mo b a ab ab ab ab Note 5% Values in each column followed by different small letters mean significantly difference at the 5% level ~ 0. 4 mg /kg mg /kg Mo 0 ~ 0. 4 mg /kg

4 Mo 0. 8 mg /kg Mo 0. 2 mg /kg Mo 0. 8 mg /kg Mo 0. 4 mg /kg 4 > > > 3 Mo 0. 2 mg /kg Mo mg /kg mg /kg

5 pharmacological activity of Paris L. J. Chin. Trad. Herb. Drugs Hisashi M Yutana P Toshio M. Protective effects of steroid saponins from Paris polyphylla var. yunnanensis on Ethanol-or indomethacin-induced gastric mucosal lesions in rats structural requirement for activity and mode of action J. Bioorg. Chem. Lett J He J Zhang S Wang H et al. Research status for Paris polyphylla var. yunnanensis J. Acta Bot. Yunnanica Fig. 5 Total saponin contents in new and old rhizomes of J paris polyphilla var. yunnanensis under different Chen C Kang P D Yang L Y et al. Appraisement and utilization Mo levels dry weight of Paris polyphylla var. yunnanensis from different geographical Note 5% Different provenances J. Southwest China J. Agric. Sci letters above the bars mean significant difference at the 5% level mg /kg 0. 8 mg /kg 8 J Mo 0. 2 mg /kg Mo 0. 4 mg /kg 3 10 Mo 0. 8 mg /kg Mo 0. 2 ~ 0. 4 mg /kg J M Li H. Botany of Paris L. M. Beijing Science Press Zhong T Y Shu G M. Study on Paris L. resource in Sichuan province J. Res. Expl. Mark J. J Wu S S Gao W Y. Research progress of chemical components and 7. J Wang L P Qi X W. Preliminary study on domestication and cultivation of Paris polyphylla var. yunnanensis J. Chin. Wild Plant Res Li S P Yang L Y Yang B. Study on high propagation efficiency and high yield cultivation of Paris polyphylla var. yunnanensis J. Southwest China J. Agric. Sci M Lu J L. University Press Plant nutrition M. Beijing Beijing Agricultural. J Liu P Yang Y A. Effect of molybdenum and boron on quality of soybean J. Sci. Agric. Sin Du Y Q Liao X R He J H et al. Effects of boron and molybdenum on growth and yield of peanut J. Plant Nutr. Fert. Sci J J. Wang L H Xu F S Wang Y H. Effects of B Mo Zn interaction on the seed yield and quality of Brassica napus J. Plant Nutr. Fert. Sci Hu C X Wang Y H Tang Q L Wei W X. Effect of molybde-

6 num and nitrogen fertilizers on free amino acid protein and its amino acid composition of winter wheat grains J. Plant Nutr. Fert. Sci J. M. Shanghai Shanghai Scientific and Technical Press Xu J Z. The effect of Mo Zn Mn B application on Angelica cultivation J. China J. Chin. Mater. Med J Su W H Zhang G F. Relationship between the photosynthesis of Xu J Z Liu X R Zhao R et al. Application of Mo Zn Mn and Paris Polyphylla var. yunnanensis and the environmental factors Fe in the cultivation of Codonopsis pilosula J. J. Chin. Med. Mater M Liu W D. Microelement nutrition and application M. Beijing Chinese Agriculture Press M Nanjing Soil Institute of CAS. Soil physical and chemical analysis 18. M Bao S D. Soil and agricultural chemistry analysis M. Beijing Chinese Agriculture Press J. J. J. Yunnan Univ M National Pharmacopoeia Council. Pharmacopoeia of the people's republic of China M. Beijing Chemistry Industry Press

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