Blood Bank Study Questions

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Lesley Sims
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1 Blood Bank Study Questions Name Date Directions: Write your answers on these sheets. You may use the back of the page or additional sheets of paper if you need more room. The total point value is indicated to the left of each question. (2 point) Describe the principle of the anti-human test (Coomb's procedure) and draw a picture to illustrate the principle. (3 points) List three (3) causes of false positives and three (3) causes of false negatives in the Coombs procedure. False Positives False Negatives Summer MLAB 2360! 1

2 (8 points) For each of the components listed below fill in the storage temperature, expiration time, and use. Storage Temp Exp. Time Use a. Packed Cells (ADSOL) b. Fresh frozen plasma (frozen) c. Red blood cells (frozen) d. Washed red blood cells e. Fresh frozen plasma (thawed) f. Cryoprecipitate (thawed) g. Cryoprecipitate (pooled) h. Platelets (pooled) (1 point) 4. What is meant by the term, weak D positive? (2 points) 5. List the deferral times for the following vaccinations. A. Measles (rubeola) B. Hepatitis B vaccine C. recently transfused D. Hepatitis B Immune Globulin (HBIG) (1 point) 6. List two (2) causes for permanent deferral of a donor. (3 points) 7. Briefly describe the cause of HDN, including the antibody class involved and the most common cause. Summer MLAB 2360! 2

3 (2 points) 8. List two (2) immediate adverse affects of transfusion. List two (2) delayed adverse affects of transfusion. immediate delayed (5 points) 9. List three (3) things that a compatible crossmatch cannot guarantee. (5 points) 10. List three (3) unexpected antibodies which are usually reactive at immediate spin. (5 points) 1 List three (3) unexpected antibodies which are usually reactive only at Coombs. (1 point) 1 Which clinically significant antigen is destroyed by ficin? (1 point) 1 What antibody, implicated in acute hemolytic transfusion reactions, has been known to be dependent on the presence of fresh complement? (2 points) 14. List four (4) conditions in which a patient may have a positive direct antiglobulin test. 4. (5 points) 15. List three (3) incidences in which D negative women would need to receive Rh immune globulin. Summer MLAB 2360! 3

4 (5 points) 16. What three (3) criteria must be met for a woman to be considered an Rh immune globulin candidate after giving birth? (2 points) 17. What is the principle of the Kleihauer Betke acid elution stain? Why is it done? (1 point) 18. Define elution. (2 points) 19. Compare and contrast the principle of the Rosette to the weak D procedure. (1 point) 20. Compare and contrast the IAT and DAT as to what is being detected. (1 point) 2 For what are the lectins Ulex europaeus and Dolichos biflorus used? (5 points) 2 What is the name of the O h phenotype? What naturally occurring antibodies are found in the serum or plasma of persons with this phenotype? If you need to transfuse a patient with this phenotype, what blood type would you use? Instructions: Refer to the attached panels numbered to answer the following questions. Each answer is worth 0.5 point. 2 A. Identify the antibody present in this patient's serum. 24. A. Identify the antibody present in this patient's serum. Summer MLAB 2360! 4

5 25. A. Identify the antibody present in this patient's serum. 26. A. Identify the antibody present in this patient's serum. B. What additional antibodies may be masked or covered up by the primary antibody? 27. A. Identify the antibody present in the albumin panel. B. Identify the antibody present in the ficin panel. C. Explain why the antibody identified in the albumin panel is non-reactive in the ficin panel. Summer MLAB 2360! 5

QUICK REFERENCE TO BLOOD BANK TESTING

QUICK REFERENCE TO BLOOD BANK TESTING All Blood bank Tests are performed on demand 24 hours a day, 7 days a week. Feto/Maternal Bleed Quantitation estimates will be available within 4 hours of blood bank