SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005
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1 SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005 EUROFINS SCIENTIFIC; NUTRITION ANALYSIS CENTER 2200 Rittenhouse Street Des Moines, IA Lisa Ruiz Phone: CHEMICAL Valid To: February 28, 2018 Certificate Number: In recognition of the successful completion of the A2LA evaluation process (including an assessment of the laboratory's compliance with the A2LA Food Testing Program Requirements, containing the 2010 AOAC International Guidelines for Laboratories Performing Microbiological and Chemical Analyses of Food and Pharmaceuticals ), accreditation is granted to this laboratory to perform the following tests on commodities, spices, foods, dietary supplements, feeds, and pet foods. Test Type/Technology: Ash AOAC AOAC c (ref ) Protein, Combustion AOCS Ba 4e-93 AOCS Ba 4f-00 AOAC AOAC Protein, Kjeltec AOAC Eurofins Method: PR-MET3368 Moisture and Volatiles by Vacuum Oven AOAC AOAC AOAC AOAC AOAC AOAC Eurofins Method: AN-MET3409 Loss on Drying (Moisture) by Force Draft Oven AOCS Ba 2a-38; Eurofins Method: PR-MET3365 AOCS Ac 2-41 AOCS Aa 3-38 AOAC AOAC AOAC AACC NFTA (NFTA Method 2.1.4) Crude Fiber AOCS Ba 6-84 AOAC Eurofins Method: PR-MET3363 (A2LA Cert. No ) 03/04/2016 Page 1 of 5
2 Dietary Fiber: Soluble, Insoluble, and Total Fiber AOAC Acid Detergent Fiber (ADF) by Filter Bag ANKOM Technology Method Technique Eurofins Method: PR-MET3366 Neutral Detergent Fiber (NDF) by Filter Bag Technique ANKOM Technology : NDF for Ankom 2000 Fiber Analyzer Eurofins Method:PR-MET3367 Crude Fat by Solvent Extraction AOAC AOAC AOCS Ba 3-38 AOCS Ac 3-44 AOCS Aa 4-38 Eurofins Method: PR-MET3373 Crude Fat by Acid and/or Alkaline Hydrolysis AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC AOAC Eurofins Method:GCLI-MET3328 Sugar Profile (Fructose, Glucose, Sucrose, Maltose, Lactose) by HPLC-ELSD AOAC Nollet, L.M.L. (Ed.) (2000). Food Analysis by HPLC. New York, NY: Marcel Dekker, Inc. (Peris-Tortjada, M. (Author) HPLC Determination of Carbohydrates in Foods Chapter 7 p ) Eurofins Method: LC-MET3319 Tryptophan by Alkaline Hydrolysis (HPLC) AOAC Eurofins Method: LC-MET3302 Amino Acids by Performic Acid Oxidation (HPLC) AOAC Cystine, Taurine and Methionine Eurofins Method: LC-MET3306 Amino Acids by Acid Hydrolysis (HPLC) AOAC Alanine, Arginine, Aspartic Acid, Glutamic Acid, Eurofins Method: LC-MET3307 Glycine, Histidine, Hydroxyproline, Isoleucine, Leucine, Phenylalanine, Proline, Lysine, Serine, Threonine, Tyrosine, Valine Scoville Heat Units by UPLC AOAC ASTA 21.3 Eurofins Method: LC-MET3311 AOAC Eurofins Method: VT-MET3391 Vitamin A: Total Vitamin A, β-carotene, and Retinol by HPLC Vitamin C by Fluorescence AOAC Eurofins Method: VT-MET3375 Ascorbic Acid (Vitamin C) and Isoascorbic Acid Journal of Food Chemistry, 94, (2006) Eurofins Method: VT-MET3396 (A2LA Cert. No ) 03/04/2016 Page 2 of 5
3 Vitamin D by HPLC Total Vitamin D, D 2 (ergocalciferol), and D 3 (cholecalciferol) EN 12821:2009 Determination of vitamin D by high performance liquid chromatography -Measurement of cholecalciferol (D3) and ergocalciferol (D2). Vitamin D by LC-MS/MS Total Vitamin D, D 2 (ergocalciferol), and D 3 (cholecalciferol) Vitamin E Profile including: Total Vitamin E, and tocopherol isomers: alpha (α), beta (β), gamma (γ), and delta (δ) by HPLC Eurofins Method: VT-MET3388 Huang et al.: Journal of AOAC International, 2012, Vol. 95, No.2, 1-3 Gilliand & Dowell: Journal of AOAC international, 2012, Vol 95, No.3, Huang etc., Rapid Commun. Mass Spectrom 2014, 28, Eurofins Method: VT-MET3401 AOAC Eurofins Method: VT-MET3382 Thiamin - Vitamin B 1 by Fluorescence Detection AOAC Eurofins Method: VT-MET3390 Riboflavin - Vitamin B 2 by Fluorometric Method AOAC Eurofins Method: VT-MET3376 Pyridoxine - Vitamin B 6 by HPLC Journal of AOAC International, 88, 30-37, (2005) Eurofins Method:VT-MET3395 Niacin - Vitamin B 3 by Microbiological Method AOAC Eurofins Method: VT-MET3379 Total Folate by Microbiological Method AOAC Eurofins Method: VT-MET3389 Biotin - Vitamin B 7 by Microbiological Method Biotin, Methods of Vitamin Assay, 3 rd edition, Interscience Publishers, 1966, chap 12 Eurofins Method: VT-MET3377 Pantothenic Acid - Vitamin B 5 by Microbiological AOAC Method Choline by HPLC-Fluorescence with Chemical Derivatization Calcium (Ca), Sodium (Na), Iron (Fe), Phosphorus (P), Potassium (K), Magnesium (Mg), Zinc (Zn), Copper (Cu), and Manganese (Mn) by ICP - OES Selenium (Se) by AAS (Atomic Absorption Spectroscopy) Sulfur by ICP-OES Eurofins method: VT-MET3381 AOAC Analytica Chimica Acta 664 (2009) Eurofins Method: VT-MET3400 AOAC AOAC Eurofins Method: EL-MET3284 AOAC AOAC Eurofins Method: EL-MET3285 AOAC Eurofins Method: EL-MET3290 T.T. Nham. Analysis of soil extracts using the Varian 725-ES, Varian ICP-OES Application Note No. 34 A. R. Jurgensen, J. C. Hart, L. L. Farrow. Sulfur limits of detection and spectral interference corrections for DWPF sludge matrices by inductively coupled plasma emission spectrometry, WSRC-TR , Z. A. Grosser, L. J. Davidowski, P. Wee. The analysis of biodiesel for inorganic contaminants, including sulfur, by ICP-OES, Application note, PerkinElmer 2009 Eurofins Method: EL-MET3289 (A2LA Cert. No ) 03/04/2016 Page 3 of 5
4 Heavy Metals by ICP-MS; Arsenic (As), Cadmium (Cd), Mercury (Hg), Lead (Pb) Method CLG-TM3.01, USDA Food Safety and Inspection Service, 2006 J. Entwisle. Determination of Mercury in Microwave Digests of Foodstuffs by ICP-MS, Application note, Agilent Technologies, 2004 Julshamn et al. Determination of Arsenic, Cadmium, Mercury, and Lead by Inductively Coupled Plasma Mass Spectrometry in Foods after Pressure Digestion: NMKL Interlaboratory Study, Journal of AOAC Int., 90, No 3, 2007 Zbinden, P. Andrey, D. Determination of Trace Element Contaminants in Food Matrices Using a Robust, Routine Analytical Method for ICP-MS, Atomic Spectroscopy, Vol. 19 (6), p Eurofins Method: EL-MET3292 Cholesterol by GC AOAC Eurofins Method: GCLI-MET3353 Fatty Acid Profile non-nlea AOCS Ce 2-66, Ce 1b-89 Fatty Acid Profile Including Total Fat, Unsaturated, Saturated, and Trans Fat by GC Omega-3 EPA and DHA Fatty Acids by GC Marine Oil Fatty Acid Profile (MOFAP) by GC Oxidative Stability Index Eurofins Method: GCLI-MET3352, GCLI-MET3339 AOAC AOAC Eurofins Method: GCLI-MET3332 GOED Voluntary Monograph AOCS Ce 1b-89, Eurofins Methods: GCLI-MET3360, GCLI-MET3339 AOCS Official Method Cd12b-92 Eurofins Method: GCLI-MET3342 Free Fatty Acid AOAC , AOCS Ca 5a-40 Eurofins Method: GCLI-MET3334 Acid Value (or Acid Number) AOAC AOCS Cd 3d-63 Eurofins Method: GCLI-MET3334 Unsaponifiable Matter AOCS Ca 6a-40 Eurofins Method: GCLI-MET3359 Saponification Value AOCS Cd 3-25 Neutral Oil Loss AOCS Ca 9f-57 Peroxide Value AOAC Cd 8-53 p-anisidine Value AOCS Cd Iodine Value AOCS Cd 1d-92 Totox Value GOED Voluntary Monograph Moisture by Toluene Distillation AOAC Steam Volatile Oil ASTA 5.0 Water Activity AquaLab Operator s manual, Version 3 for AquaLab Model Series 3TE Eurofins Method AN-MET3406 Capillary Melting Point AOCS Cc 1-25, Eurofins Method: GCLI-MET3330 Calories from Fat and Saturated Fat Calculated using the Atwater (4.9.4) formula, as cited in 21CFR101.9(c)(1)(A&B) (A2LA Cert. No ) 03/04/2016 Page 4 of 5
5 Total Calories Calculated using the Atwater (4.9.4) formula, as cited in 21CFR101.9(c)(1)(A&B) or As cited in 21CFR101.9(c)(1)(i)(C) Total Carbohydrates Calculated by difference, as cited in 21CFR101.9(c)(6) ASTA Color ASTA 20.1 (A2LA Cert. No ) 03/04/2016 Page 5 of 5
6 Accredited Laboratory A2LA has accredited EUROFINS SCIENTIFIC; NUTRITION ANALYSIS CENTER Des Moines, IA for technical competence in the field of Chemical Testing This laboratory is accredited in accordance with the recognized International Standard ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories. This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management system (refer to joint ISO-ILAC-IAF Communiqué dated 8 January 2009). Presented this 4 th day of March Senior Director of Quality and Communications For the Accreditation Council Certificate Number Valid to February 28, 2018 For the tests to which this accreditation applies, please refer to the laboratory s Chemical Scope of Accreditation.
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