Preamble. 1. If it is necessary, are you willing to receive follow up questions to help clarify any answers to the survey questions?

Transcription

1 Preamble The purpose of this inter organizational survey is to gather data regarding transplant center practices for preparing cord blood units for administration, including characterizing and confirming the identity of cord blood units and thawing, diluting, and washing the units. You will be asked 34 questions; some questions relate to four different scenarios regarding cord blood units. After using this version to compile answers to the questions, submit your responses online at The time to complete the survey is estimated to be about 20 minutes after compiling all information for your transplant center. 1. If it is necessary, are you willing to receive follow up questions to help clarify any answers to the survey questions? Yes No, I wish to remain anonymous. Page 1

2 Contact Information (Optional) 2. Name (optional) 3. (optional) 4. Region of the World North America Europe Asia Australia/New Zealand Central America/South America Africa 5. Transplant Center Size by Number of Cord Blood Transplants per Year (Adult and Pediatric) < Does your center consider non inherited maternal antigens (NIMA) in cord selection when the unit is not a 6/6 match? Always Most of the time Occasionally Never Comment 5 6 Page 2

3 Testing of the Cord Blood Unit After Receipt and Prior to Infusion 7. Please indicate below all standard testing performed AT YOUR CENTER after receipt of the ENTIRE cord blood unit (CBU) and prior to administration. (Check all that apply.) Sample taken prior to infusion Results required prior to infusion TNC WBC Differential CD34 enumeration Viability (any method) ABO typing (with or without Rh) Platelet count Hematocrit/Hemoglobin HLA HLA antibody CFU None 8. If viability is performed, what method is used? Trypan blue 7 AAD We do not perform viability Other 5 6 Page 3

4 Scenario 1 For questions 9 12, assume that CBU confirmatory typing was performed via the registry or cord blood bank on an attached segment prior to shipment to your center. 9. Which tests are performed after receipt and prior to administration to confirm identity of the CBU? (Select all that apply.) ABO typing (with or without Rh) HLA None required prior to administration for this scenario 10. For the identity tests described in question 9, what source of CBU material does your center prefer to use (assume all types of materials are available for the CBU)? Attached segment material only Any material (ancillary or attached) associated with the cord blood unit No identity testing performed at my center is required prior to administration for this scenario 11. For pre administration HLA identity testing, please check all standard loci tested: HLA A HLA B HLA C HLA DRB1 HLA DQB1 HLA DPB1 No HLA identity testing performed prior to infusion for this scenario 12. For pre administration HLA identity testing, please indicate the typing methodology: Serology for all classes of loci Serology for Class I loci, DNA based for Class II loci DNA based for all classes of loci RT PCR No HLA identity testing done prior to administration for this scenario Page 4

5 Scenario 2 For questions 13 16, assume that CB confirmatory typing was performed on ancillary material but not on an attached segment prior to shipment to your center and an attached segment is available. 13. Which tests are performed after receipt and prior to administration to confirm identity of the CBU? (Select all that apply.) ABO typing (with or without Rh) HLA None required prior to administration for this scenario 14. For the identity tests described in question 13, what source of CBU material does your center prefer to use (assume all types of materials are available for the CBU)? Attached segment material only Any material (ancillary or attached) associated with the cord blood unit No identity testing performed at my center is required prior to administration for this scenario 15. For pre administration HLA identity testing, please check all standard loci tested: HLA A HLA B HLA C HLA DRB1 HLA DQB1 HLA DPB1 No HLA identity testing performed prior to administration for this scenario 16. For pre administration HLA identity testing, please indicate the typing methodology: Serology for all classes of loci Serology for Class I loci, DNA based for Class II loci DNA based for all classes of loci RT PCR No HLA identity testing done prior to administration for this scenario Page 5

6 Scenario 3 For questions 17 22, assume that extended typing was performed on ancillary material but not on an attached segment prior to shipment. Ancillary material is available but NO attached segment is available. 17. If this CBU was the best HLA match and dose for a patient, would your center accept it? Yes No Page 6

7 Scenario Which tests are performed after receipt and prior to administration to confirm identity of the CBU? (Select all that apply.) ABO typing (with or without Rh) HLA None required prior to administration for this scenario 19. For the identity tests described in question 18, what source of CBU material does your center prefer to use (assume all types of materials are available for the CBU)? Aliquot of thawed CBU Any material associated with the cord blood unit No identity testing performed at my center is required prior to administration for this scenario 20. For pre administration HLA identity testing, please check all standard loci tested: HLA A HLA B HLA C HLA DRB1 HLA DQB1 HLA DPB1 No HLA identity testing performed at my center prior to administration for this scenario 21. For pre administration HLA identity testing, please indicate the typing methodology: Serology for all classes of loci Serology for Class I loci, DNA based for Class II loci DNA based for all classes of loci RT PCR No HLA identity testing performed at my center prior to administration for this scenario Page 7

8 22. Which tests are performed after administration to confirm identity of the CBU? (Select all that apply.) ABO typing only (with or without Rh) HLA typing only Both HLA and ABO typing Post administration identity testing not performed in this scenario Page 8

9 Scenario 4 For questions 23 28, assume that extended typing was performed on ancillary material but not on an attached segment prior to shipment; no further material is available. 23. If this CBU was the best HLA match and dose for a patient, would your center accept it? Yes No Page 9

10 Scenario Are any tests performed after receipt and prior to administration to confirm identity of the CBU in this scenario? Yes, an aliquot of the thawed CBU would be used Not for this scenario 25. Which tests are performed after receipt and prior to administration to confirm identity of the CBU? (Select all that apply.) ABO typing (with or without Rh) HLA None required prior to administration for this scenario 26. For pre administration HLA identity testing, please check all standard loci tested: HLA A HLA B HLA C HLA DRB1 HLA DQB1 HLA DPB1 No HLA identity testing performed prior to administration for this scenario 27. For pre administration HLA identity testing, please indicate the typing methodology: Serology for all classes of loci Serology for Class I loci, DNA based for Class II loci DNA based for all classes of loci RT PCR No HLA identity testing done prior to administration for this scenario Page 10

11 28. Which tests are performed after administration to confirm identity of the CBU? (Select all that apply.) ABO typing only (with or without Rh) HLA typing only Both HLA and ABO typing Post administration identity testing not performed in this scenario Page 11

12 Post Administration Testing 29. When does your center perform post administration identity testing (HLA and/or ABO)? For all CBUs When identity could not be performed pre administration via an attached segment or bag wash When identity could not be confirmed pre administration via ancillary material Never 30. When post administration identity testing is performed, please indicate which type of testing is performed: ABO only (with or without Rh) HLA by serology only HLA by DNA methods with or without serology HLA with RT PCR ABO and/or HLA Post administration identity testing is not performed Page 12

13 Thawing, Washing, and Dilution 31. When do you thaw CBUs? Prior to administration in the processing facility At the time of infusion at the bedside Either, depending on the circumstances 32. For double cord blood transplants, do you wait to thaw the second unit until after the first unit has been administered? Always Most of the time Occasionally Never We do not perform double cord blood transplants. 33. In the table below, please indicate the preparative method(s) used at your center for a given type of cord blood unit. Thaw and Infuse No Processing Thaw and Dilute (for example, Albumin/Dextran) Thaw and Wash Red Cell Replete Cord Blood Unit Dextran Containing Cord Blood Unit RBC Deplete Cord Blood Unit Small Volume (less than 30 ml) RBC Deplete Cord Blood Unit Large Volume (greater than or equal to 30 ml) 34. What is your washing and/or dilution method? 5 6 Page 13