sirna delivery targeting specific sites in vivo Yvette Zarb JC
|
|
|
- Brianne Oliver
- 5 years ago
- Views:
Transcription
1 sirna delivery targeting specific sites in vivo Yvette Zarb JC
2 Highly conserved biological process sirna sirna guides an RNA-inducing silencing protein complex Laufer, Detzer, Sezakiel & Restle, RNA Technologies and their Applications Degrading the mrna with the matching sequence
3 Challenges in RNAi in vivo Shim & Kwon, FEBS Journal
4 Challenges in RNAi in vivo Shim & Kwon, FEBS Journal
5
6 Physical chemistry of bone targeting molecules Bone resorption Bone formation Oligopeptides Bisphosphonates? Adapted from Manolagas, O Brien & Almeida, Nature review endocrinology
7 Physical chemistry of bone targeting molecules (AspSerSer) 6 Oligopeptide potential to target boneformation surfaces Preferentially binds to lowly crystallized hydroxyapatite and amorphous calcium phosphonate (Asp 8 ) Oligopeptide preferentially bind to bone-resorption surfaces stronger affinity to highly crystallized hydroxyapatite
8 Differential occupancy characteristics of moieties at bone-formation or -resorption surfaces Xylenol orange marks bone formation surfaces (AspSerSer) 6 targets bone formation surfaces (Asp 8 ) target bone resorption surfaces
9 Characterization of the targeted delivery system in vivo
10 Organ-selective gene knockdown in vivo
11 Cell-selective delivery and knockdown efficiency in vivo
12 In vivo microct examinations of the threedimensional trabecular architecture in healthy rats relative bone volume (BV/TV) trabecular thickness (Tb.Th) trabecular number (Tb.N) connectivity density (Conn.D) structure model index (SMI) trabecular space (Tb.Sp)
13 In vivo microct examination of the three-dimensional trabecular architecture in OVX-treated rats
14 Summary Rosen, Nature Medicine
15
16 Polyethyleneimine (PEI) Polymer class investigated as a gene delivery material High molecular weight PEI (approx. 25,000KDa): assoiciated with off-target effects Low molecular weight PEI (approx. 600KDa): well tolerated but cannot facilitate sirna delivery
17 7C1 synthesis 7C1 nanoparticles were mixed with C 14 PEG 2000 sirna in a high-throughput microfluidic chamber
18 7C1 Characterization Average 7C1 hydrodynamic diameter measured by dynamic light scattering, and weighted by volume 6,P- toluidinylnapthalene-2- sulfonate (TNS) fluorescence of formulated 7C1 nanoparticles function of ph (used to measure 7C1 pka)
19 7C1 in vivo biodistribution Alexa647-tagged sirna complexed to 7C1, 1 h after intravenous injection. Serum Cy5.5 concentration following injection with 7C1-Cy5.5 sirna naked Cy5.5 sirna. Time points were selected to measure systemic sirna accumulation after Cy5.5 was cleared from serum.
20 7C1 delivers sirna to endothelial HMVEC cells following 7C1- Alexa647 treatment in the presence of small molecules inhibiting: clathrin (Chlorpromazine) caveolin (Fillipin) both endocytotic pathways (Dynasore) cells
21 7C1 delivers sirna to endothelial Evans Blue Dye (EBD) pulmonary absorbance 7 and 14 days following a 0.6 mg kg -1 injection of 7C1- sivecad. Target/GapDH mrna ratios (normalized to PBS-treated mice) following injection of 7C1 formulated with: sicntrol ICAM-2 Tie2 VE-cadherin VEGFR2 Tie1 cells
22 7C1 preferentially delivers sirna to pulmonary endothelial cells in vivo Isolated from mice after treatment with 7C1 formulated with Alexa647-tagged sirna. pulmonary endothelial (CD31 + ) Haematopoietic (CD45 + ) epithelial (CD326 + ) B cells (CD19 + ) T cells (TCRb + )
23 7C1 preferentially delivers sirna to pulmonary endothelial cells in vivo Hepatocyte specific: Factor 7 two days following treatment with liver-targeting molecule Hepat01-siFactor7 or 7C1- sifactor7. Particles were formulated with different 7C1:cholesterol:C 14 PEG 2000 molar ratios efficacy can vary with the molar ratio of PEG and cholesterol Expression in pulmonary, renal and hepatic endothelium without reducing F7 mrna expression. CD45 median fluorescent intensity following treatment
24 7C1 preferentially delivers sirna to pulmonary endothelial cells in vivo Mean linear intercept (MLI) between alveoli, pulmonary surface/volume ratio, total volume and pulmonary histology following two 0.5 mg kg -1 injections of sicntrol or sivegfr2. Increased MLI, alveolar volume, decreased surface/volume ratios and constant infiltrating myeloid cells consistent with an induced emphysema-like phenotype
25 7C1-mediated mrna silencing modifies endothelial function in vivo Primary Lewis lung carcinoma (LLC) growth following three 1.0 mg kg -1 Number of pulmonary surface metastases following four 1.0 mg kg -1 injections To measure effects independent of primary tumour growth, animals were not treated until after primary tumour resection.
26 7C1 in publications
27 Thanks!
TOOLS sirna and mirna. User guide
TOOLS sirna and mirna User guide Introduction RNA interference (RNAi) is a powerful tool for suppression gene expression by causing the destruction of specific mrna molecules. Small Interfering RNAs (sirnas)
Gene Silencing Oligos (GSOs) Third Generation Antisense
Gene Silencing Oligos (GSOs) Third Generation Antisense Walter R. Strapps, Ph.D. Executive Director, RNA Therapeutics Idera Pharmaceuticals Cambridge, MA NASDAQ: IDRA www.iderapharma.com Idera is a leader
RNAi. Martin Latterich
RNAi Martin Latterich Contributors Abbreviations Preface i x ( xi xiii 1 Methods in RNA interference 1 Martin Latterich and Dalia Halawan i References 2 2 RNAi reagent design 3 Bernd Jagla and Nathalie
H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland School of Medicine Baltimore, Md
Major Advances in Cancer Prevention, Diagnosis and Treatment~ Why Mesothelioma Leads the Way H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland
Dicer Substrate RNAi Design
INTEGRATED DNA TECHNOLOGIES, INC. Dicer Substrate RNAi Design How to design and order 27-mer Dicer-substrate Duplex RNAs for use as RNA interference reagents The following document provides a summary of
Diabetes and Drug Development
Diabetes and Drug Development Metabolic Disfunction Leads to Multiple Diseases Hypertension ( blood pressure) Metabolic Syndrome (Syndrome X) LDL HDL Lipoproteins Triglycerides FFA Hyperinsulinemia Insulin
Absorption of Drugs. Transport of a drug from the GI tract
Absorption of Drugs Absorption is the transfer of a drug from its site of administration to the bloodstream. The rate and efficiency of absorption depend on the route of administration. For IV delivery,
Microarray Technology
Microarrays And Functional Genomics CPSC265 Matt Hudson Microarray Technology Relatively young technology Usually used like a Northern blot can determine the amount of mrna for a particular gene Except
岑 祥 股 份 有 限 公 司 技 術 專 員 費 軫 尹 20100803
技 術 專 員 費 軫 尹 20100803 Overview of presentation Basic Biology of RNA interference Application of sirna for gene function? How to study mirna? How to deliver sirna and mirna? New prospects on RNAi research
Understanding the immune response to bacterial infections
Understanding the immune response to bacterial infections A Ph.D. (SCIENCE) DISSERTATION SUBMITTED TO JADAVPUR UNIVERSITY SUSHIL KUMAR PATHAK DEPARTMENT OF CHEMISTRY BOSE INSTITUTE 2008 CONTENTS Page SUMMARY
MicroRNA formation. 4th International Symposium on Non-Surgical Contraceptive Methods of Pet Population Control
MicroRNA formation mirna s are processed from several precursor stages Mammalian genomes seem to have 100 s of mirna s Nucleotides in positions 2-8 of an mirna are considered the mirna seed 5 Methyl-G
Outline. interfering RNA - What is dat? Brief history of RNA interference. What does it do? How does it work?
Outline Outline interfering RNA - What is dat? Brief history of RNA interference. What does it do? How does it work? What is RNA interference? Recently discovered regulatory level. Genome immune system.
Nanomedisin hos GE Healthcare Fra forskning til kommersialisering. 12.12-07 Per A Foss
hos GE Healthcare Fra forskning til kommersialisering Per A Foss Introduction GE and GE Healthcare Early health / personalized medicine Molecular imaging anoparticles Idea to market 2/ GE 6 diverse global
Uses of Flow Cytometry
Uses of Flow Cytometry 1. Multicolour analysis... 2 2. Cell Cycle and Proliferation... 3 a. Analysis of Cellular DNA Content... 4 b. Cell Proliferation Assays... 5 3. Immunology... 6 4. Apoptosis... 7
MicroRNA Mike needs help to degrade all the mrna transcripts! Aaron Arvey ISMB 2010
Target mrna abundance dilutes microrna and sirna activity MicroRNA Mike needs help to degrade all the mrna transcripts! Aaron Arvey ISMB 2010 Target mrna abundance dilutes microrna and sirna activity Erik
Biosafety considerations of new dsrna molecules
Biosafety considerations of new dsrna molecules GM-free Europe Conference 2015 May 6-8 Berlin, Germany Dr. Sarah Agapito Current GMOs Plant transformed to produce new protein A G C U G G C A A U G U C
The world of non-coding RNA. Espen Enerly
The world of non-coding RNA Espen Enerly ncrna in general Different groups Small RNAs Outline mirnas and sirnas Speculations Common for all ncrna Per def.: never translated Not spurious transcripts Always/often
Manual for: sirna-trans Maximo Reagent
Manual for: sirna-trans Maximo Reagent Contact: info@geneon.net; www.geneon.net GeneON GmbH Germany GeneON GmbH / Protocol sirna-trans reagent vs. 1.3 / www.geneon.net / - 0 - sirna-trans reagent Instruction
Supplemental Information. McBrayer et al. Supplemental Data
1 Supplemental Information McBrayer et al. Supplemental Data 2 Figure S1. Glucose consumption rates of MM cell lines exceed that of normal PBMC. (A) Normal PBMC isolated from three healthy donors were
Teriflunomide is the active metabolite of Leflunomide, a drug employed since 1994 for the treatment of rheumatoid arthritis (Baselt, 2011).
Page 1 of 10 ANALYTE NAME AND STRUCTURE TERIFLUNOMIDE Teriflunomide TRADE NAME Aubagio CATEGORY Antimetabolite TEST CODE PURPOSE Therapeutic Drug Monitoring GENERAL RELEVANCY BACKGROUND sclerosis. The
Translation Study Guide
Translation Study Guide This study guide is a written version of the material you have seen presented in the replication unit. In translation, the cell uses the genetic information contained in mrna to
Production of antigens and antibodies in plants: alternative technology?
Production of antigens and antibodies in plants: alternative technology? George Lomonossoff John Innes Centre Norwich, UK ECOPA, Alicante 29 th Sept. 2006 Why use Plants as Biofactories? Produce large
RNAi Shooting the Messenger!
RNAi Shooting the Messenger! Bronya Keats, Ph.D. Department of Genetics Louisiana State University Health Sciences Center New Orleans Email: bkeats@lsuhsc.edu RNA interference (RNAi) A mechanism by which
TECHNICAL INSIGHTS TECHNOLOGY ALERT
TECHNICAL INSIGHTS TECHNOLOGY ALERT UNLOCKING BREAST CANCER TUMORS' DRUG RESISTANCE A troubling phenomenon in breast cancer treatment is that some patients tumors become resistant to treatments over time.
Gene Therapy- Past, Present and Future. Mark A. Kay MD, PhD Dennis Farrey Family Professor Departments of Pediatrics and Genetics Stanford University
Gene Therapy- Past, Present and Future Mark A. Kay MD, PhD Dennis Farrey Family Professor Departments of Pediatrics and Genetics Stanford University Definition of gene therapy Gene therapy is the introduction
IKDT Laboratory. IKDT as Service Lab (CRO) for Molecular Diagnostics
Page 1 IKDT Laboratory IKDT as Service Lab (CRO) for Molecular Diagnostics IKDT lab offer is complete diagnostic service to all external customers. We could perform as well single procedures or complex
OPG-Fc inhibits ovariectomy-induced growth of disseminated breast cancer cells in bone.
Title of the poster Authors Sheffield Cancer Research Centre University of Sheffield OPG-Fc inhibits ovariectomy-induced growth of disseminated breast cancer cells in bone. Dr Penelope Ottewell Background
2.1.2 Characterization of antiviral effect of cytokine expression on HBV replication in transduced mouse hepatocytes line
i 1 INTRODUCTION 1.1 Human Hepatitis B virus (HBV) 1 1.1.1 Pathogenesis of Hepatitis B 1 1.1.2 Genome organization of HBV 3 1.1.3 Structure of HBV virion 5 1.1.4 HBV life cycle 5 1.1.5 Experimental models
COMMITTEE FOR MEDICINAL PRODUCTS FOR HUMAN USE (CHMP) REFLECTION PAPER
European Medicines Agency London 23 April 2009 EMEA/CHMP/BMWP/102046/2006 COMMITTEE FOR MEDICINAL PRODUCTS FOR HUMAN USE (CHMP) REFLECTION PAPER NON-CLINICAL AND CLINICAL DEVELOPMENT OF SIMILAR MEDICINAL
MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project
Mabsolus-2015-UK:Mise en page 1 03/07/15 14:13 Page1 Services provider Department of MABSolys from conception to validation MAB Solut is involved at each stage of your project Creation of antibodies Production
Supporting Information
Supporting Information Wiley-VCH 27 69451 Weinheim, Germany Blockage of sirna Binding to a p19 Viral Suppressor of RNA Silencing by Cysteine Alkylation Selena M. Sagan, Roger Koukiekolo, Elisabeth Rodgers,
Notch 1 -dependent regulation of cell fate in colorectal cancer
Notch 1 -dependent regulation of cell fate in colorectal cancer Referees: PD Dr. Tobias Dick Prof. Dr. Wilfried Roth http://d-nb.info/1057851272 CONTENTS Summary 1 Zusammenfassung 2 1 INTRODUCTION 3 1.1
CONTRACTING ORGANIZATION: University of Alabama at Birmingham Birmingham, AL 35294
AD Award Number: W81XWH-08-1-0030 TITLE: Regulation of Prostate Cancer Bone Metastasis by DKK1 PRINCIPAL INVESTIGATOR: Gregory A. Clines, M.D., Ph.D. CONTRACTING ORGANIZATION: University of Alabama at
Beneficial effects of CCR1 blockade on the progression of chronic allograft nephropathy
Beneficial effects of CCR1 blockade on the progression of chronic allograft nephropathy J. Bedke 1,2, E. Kiss 2, L. Schaefer 3, M. Bonrouhi 2, N. Gretz 4, H.-J. Gröne 2 1 Klinik für Urologie, Universität
Pharmacology of the Respiratory Tract: COPD and Steroids
Pharmacology of the Respiratory Tract: COPD and Steroids Dr. Tillie-Louise Hackett Department of Anesthesiology, Pharmacology and Therapeutics University of British Columbia Associate Head, Centre of Heart
CHEMISTRY STANDARDS BASED RUBRIC ATOMIC STRUCTURE AND BONDING
CHEMISTRY STANDARDS BASED RUBRIC ATOMIC STRUCTURE AND BONDING Essential Standard: STUDENTS WILL UNDERSTAND THAT THE PROPERTIES OF MATTER AND THEIR INTERACTIONS ARE A CONSEQUENCE OF THE STRUCTURE OF MATTER,
Transfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.
Transfection Key words: Transient transfection, Stable transfection, transfection methods, vector, plasmid, origin of replication, reporter gene/ protein, cloning site, promoter and enhancer, signal peptide,
Real-time PCR: Understanding C t
APPLICATION NOTE Real-Time PCR Real-time PCR: Understanding C t Real-time PCR, also called quantitative PCR or qpcr, can provide a simple and elegant method for determining the amount of a target sequence
Chapter 3. Protein Structure and Function
Chapter 3 Protein Structure and Function Broad functional classes So Proteins have structure and function... Fine! -Why do we care to know more???? Understanding functional architechture gives us POWER
Long-Term Effects of Drug Addiction
Long-Term Effects of Drug Addiction Part 1: Addiction is a chronic disease Drug addiction is considered a chronic brain disease because drugs cause long-lasting changes in brain structure and function.
BCOR101 Midterm II Wednesday, October 26, 2005
BCOR101 Midterm II Wednesday, October 26, 2005 Name Key Please show all of your work. 1. A donor strain is trp+, pro+, met+ and a recipient strain is trp-, pro-, met-. The donor strain is infected with
HiPerFect Transfection Reagent Handbook
Fifth Edition October 2010 HiPerFect Transfection Reagent Handbook For transfection of eukaryotic cells with sirna and mirna Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the
Normal values of IGF1 and IGFBP3. Kučera R., Vrzalová J., Fuchsová R., Topolčan O., Tichopád A.
Normal values of IGF1 and IGFBP3 Kučera R., Vrzalová J., Fuchsová R., Topolčan O., Tichopád A. Agenda of the presentation IGF1 and IGFBP3 basic characteristic Why normal values Groups of the persons and
Lezioni Dipartimento di Oncologia Farmacologia Molecolare. RNA interference. Giovanna Damia 29 maggio 2006
Lezioni Dipartimento di Oncologia Farmacologia Molecolare RNA interference Giovanna Damia 29 maggio 2006 RNA INTERFERENCE Sequence-specific gene suppression by dsrnas Gene silencing by dsrna: C. elegans
Activity 4 Long-Term Effects of Drug Addiction
Activity 4 Long-Term Effects of Drug Addiction Core Concept: Addictive drugs may lead to long-term changes in brain function. Class time required: Approximately 60-80 minutes Teacher Provides: Copy of
Appendix 2 Molecular Biology Core Curriculum. Websites and Other Resources
Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer http://www.insidecancer.org/ From the Dolan DNA Learning Center Cold
RNAi: principle DNA RNA PROTEIN
RNAi: principle RNAi DNA RNA PROTEIN Transcription (nucleus) Translation (cytoplasm) Many diseases develop from the undesirable production of specific proteins (oncogene products, mutant proteins, toxins,
Workshop 14-16 February 2006
Theoretical and practical approaches of Hepatocyte primary culture Workshop 14-16 February 2006 Lecture (2) Disaggregation & purification of target cells Coarse organizer Dr. Abo bakr Mohamed Eltayeb General
Micro RNAs: potentielle Biomarker für das. Blutspenderscreening
Micro RNAs: potentielle Biomarker für das Blutspenderscreening micrornas - Background Types of RNA -Coding: messenger RNA (mrna) -Non-coding (examples): Ribosomal RNA (rrna) Transfer RNA (trna) Small nuclear
Recombinant DNA and Biotechnology
Recombinant DNA and Biotechnology Chapter 18 Lecture Objectives What Is Recombinant DNA? How Are New Genes Inserted into Cells? What Sources of DNA Are Used in Cloning? What Other Tools Are Used to Study
Introduction to Enteris BioPharma
Introduction to Enteris BioPharma Enteris BioPharma Intelligent Solutions for Oral Drug Delivery Privately held, New Jersey based biotech company Owned solely by Victory Park Capital, a large Chicago based
Introduction to flow cytometry
Introduction to flow cytometry Flow cytometry is a popular laser-based technology. Discover more with our introduction to flow cytometry. Flow cytometry is now a widely used method for analyzing the expression
Covalent Conjugation to Cytodiagnostics Carboxylated Gold Nanoparticles Tech Note #105
Covalent Conjugation to Cytodiagnostics Carboxylated Gold Nanoparticles Tech Note #105 Background Gold nanoparticle conjugates have been widely used in biological research and biosensing applications.
AP Biology Essential Knowledge Student Diagnostic
AP Biology Essential Knowledge Student Diagnostic Background The Essential Knowledge statements provided in the AP Biology Curriculum Framework are scientific claims describing phenomenon occurring in
Seattle. Case Presentations. Case 1. 76 year old female with a history of breast cancer 12 years ago. Now presents with a pleural effusion.
Seattle Montreal IAP September 2006 Case Presentations Allen M. Gown, M.D. Medical Director and Chief Pathologist PhenoPath Laboratories Clinical Professor of Pathology University of British Columbia Case
Head of College Scholars List Scheme. Summer Studentship. Report Form
Head of College Scholars List Scheme Summer Studentship Report Form This report should be completed by the student with his/her project supervisor. It should summarise the work undertaken during the project
The immunogenic vaccine against HCV and HBV
National Centre for Research and Development, The immunogenic vaccine against HCV and HBV Katarzyna Grzyb Laboratory of Virus Molecular Biology Intercollegate Faculty of Biotechnology UG-MUG 1 Hepatitis
CUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation
CUSTOM ANTIBODIES Highly competitive pricing without compromising quality. Rat monoclonal antibodies for the study of gene expression and proteomics in mice and in mouse models of human diseases available.
PHOSPHATE-SANDOZ Tablets (High dose phosphate supplement)
1 PHOSPHATE-SANDOZ Tablets (High dose phosphate supplement) PHOSPHATE-SANDOZ PHOSPHATE-SANDOZ Tablets are a high dose phosphate supplement containing sodium phosphate monobasic. The CAS registry number
The Effect of Myeloma Cells on Bone Metabolism is Heterogenous and Correlates with Underlying Genetic Lesions and Bone Disease In Vivo
The Effect of Myeloma Cells on Bone Metabolism is Heterogenous and Correlates with Underlying Genetic Lesions and Bone Disease In Vivo Deepika Kassen 1, Neil Rabin 1, Darren Lath 2, Peter Croucher 2 and
dependent independent claims
Mechanics of claim drafting Karin Pramberger Belgrade 16/17 Nov 2006 1 dependent independent claims 1. all essential features in independent claims with at least 1 novelty conferring feature 2. fall back
Thermo Scientific Dharmacon ON-TARGETplus sirna The Standard for sirna Specificity
Thermo Scientific Dharmacon sirna The Standard for sirna Specificity Reduce off-targets up to 90% with the market-leading sirna Combine higher target specificity with guaranteed silencing Increase confidence
Ventilation Perfusion Relationships
Ventilation Perfusion Relationships VENTILATION PERFUSION RATIO Ideally, each alveolus in the lungs would receive the same amount of ventilation and pulmonary capillary blood flow (perfusion). In reality,
Summary of the risk management plan (RMP) for Orkambi (lumacaftor and ivacaftor)
EMA/662624/2015 Summary of the risk management plan (RMP) for Orkambi (lumacaftor and ivacaftor) This is a summary of the risk management plan (RMP) for Orkambi, which details the measures to be taken
sirna Duplexes & RNAi Explorer
P r o d u c t S p e c i f i c a t i o n s Guaranteed RNAi Explorer kit with Fl/Dabcyl Molecular Beacon Catalog No.:27-6402-01 Guaranteed RNAi Explorer kit with Fluorescein/Tamra TaqMan Catalog No.:27-6402-01
Safe Nano Design Molecule Manufacturing Market
August 14 16, 212 College of Nanoscale Science & Engineering (CNSE) of the University at Albany National Institute for Occupational Safety and Health (NIOSH) Prevention through Design Program Safe Nano
BioMmune Technologies Inc. Corporate Presentation 2015
BioMmune Technologies Inc Corporate Presentation 2015 * Harnessing the body s own immune system to fight cancer & other autoimmune diseases BioMmune Technologies Inc. (IMU) ABOUT A public biopharmaceutical
ncounter Leukemia Fusion Gene Expression Assay Molecules That Count Product Highlights ncounter Leukemia Fusion Gene Expression Assay Overview
ncounter Leukemia Fusion Gene Expression Assay Product Highlights Simultaneous detection and quantification of 25 fusion gene isoforms and 23 additional mrnas related to leukemia Compatible with a variety
Antigenic variation in Plasmodium falciparum : Erythrocyte invasion and immune escape mechanisms
Antigenic variation in Plasmodium falciparum : Erythrocyte invasion and immune escape mechanisms Introduction Why does immunity to malaria take so long to develop? The parasite s survival depends on its
STUDY TITLE: BONE METASTASES FROM PROSTATE CANCER EXPERIMENTAL MODEL
STUDY TITLE: BONE METASTASES FROM PROSTATE CANCER EXPERIMENTAL MODEL Updated 2011/01/11 Protocol study Animals: Age: Sex: Strain: Supplier: Rats 5 weeks old (upon delivery) Male Sprague Dawley Charles
Protein Expression. A Practical Approach J. HIGGIN S
Protein Expression A Practical Approach S. J. HIGGIN S B. D. HAMES List of contributors Abbreviations xv Xvi i 1. Protein expression in mammalian cell s Marlies Otter-Nilsson and Tommy Nilsso n 1. Introduction
FINAL REPORT For Japan-Korea Joint Research Project AREA
(Form4-2) FINAL REPORT For Japan-Korea Joint Research Project AREA 1. Mathematics & Physics 2. Chemistry & Material Science 3. Biology 4. Informatics & Mechatronics 5. Geo-Science & Space Science 6. Medical
The Cellular Response of the Reaction to the Electromagnetic Field. Frequency at 2.4 G Hertz
The Cellular Response of the Reaction to the Electromagnetic Field Frequency at 2.4 G Hertz Hsien-Chiao Teng Department of Electrical Engineering, Chinese Military Academy, Fengshan, Kaohsiung, Taiwan
Viral Infection: Receptors
Viral Infection: Receptors Receptors: Identification of receptors has come from expressing the gene for the receptor in a cell to which a virus does not normally bind -OR- By blocking virus attachment
MOLOGEN AG. Q1 Results 2015 Conference Call Dr. Matthias Schroff Chief Executive Officer. Berlin, 12 May 2015
Q1 Results 2015 Conference Call Dr. Matthias Schroff Chief Executive Officer Berlin, 12 May 2015 V1-6 Disclaimer Certain statements in this presentation contain formulations or terms referring to the future
Background. t 1/2 of 3.7 4.7 days allows once-daily dosing (1.5 mg) with consistent serum concentration 2,3 No interaction with CYP3A4 inhibitors 4
Abstract No. 4501 Tivozanib versus sorafenib as initial targeted therapy for patients with advanced renal cell carcinoma: Results from a Phase III randomized, open-label, multicenter trial R. Motzer, D.
micrornas Non protein coding, endogenous RNAs of 21-22nt length Evolutionarily conserved
microrna 2 micrornas Non protein coding, endogenous RNAs of 21-22nt length Evolutionarily conserved Regulate gene expression by binding complementary regions at 3 regions of target mrnas Act as negative
Bisphosphonate therapy. osteonecrosis of the jaw
I overview Bisphosphonate therapy and osteonecrosis of the jaw Authors_Johannes D. Bähr, Prof. Dr Dr Peter Stoll & Dr Georg Bach, Germany _Introduction Fig. 1_Structural formula of pyrophosphate and basic
Introduction to Flow Cytometry
Outline Introduction to Flow Cytometry Basic Concept of Flow Cytometry Introduction to Instrument Subsystems Daisy Kuo Assistant Product Manager E-mail: daisy_kuo@bd.com BDBiosciences Application Examples
Guidance. 2. Definitions. 1. Introduction
Work with naked DNA or RNA (including oligonucleotides, sirna, mirna, sequences that code for highly biologically active molecules and full length viral genomes) Guidance 1. Introduction The following
A customizable ADCC assay service for antibodies & fusion proteins.
Antibody Dependent Cell- Mediated Cytotoxicity (ADCC) Assay A customizable ADCC assay service for antibodies & fusion proteins. Our ADCC assay service accurately detects cell lysis based on LDH-release.
Biology 12 June 2003 Provincial Examination
Biology 12 June 2003 rovincial Examination ANWER KEY / CORING GUIDE CURRICULUM: Organizers 1. Cell Biology 2. Cell rocesses and Applications 3. Human Biology ub-organizers A, B, C, D E, F, G, H I, J, K,
Biotechnology. Srivatsan Kidambi, Ph.D.
Stem Stem Cell Cell Engineering-What, Biology and it Application Why, How?? to Biotechnology Srivatsan Kidambi, Ph.D. Assistant Professor Department of Chemical & Biomolecular Engineering University of
How many of you have checked out the web site on protein-dna interactions?
How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss
Gene Therapy. The use of DNA as a drug. Edited by Gavin Brooks. BPharm, PhD, MRPharmS (PP) Pharmaceutical Press
Gene Therapy The use of DNA as a drug Edited by Gavin Brooks BPharm, PhD, MRPharmS (PP) Pharmaceutical Press Contents Preface xiii Acknowledgements xv About the editor xvi Contributors xvii An introduction
Osteoblast Differentiation and Mineralization
Osteoblast Differentiation and Mineralization Application Note Background Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. They develop from mesenchymal precursors.
Why is HDL Cholesterol Low in People with Insulin Resistance and Type 2 Diabetes Mellitus?
Why is HDL Cholesterol Low in People with Insulin Resistance and Type 2 Diabetes Mellitus? Henry Ginsberg, MD Columbia University College of Physicians and Surgeons Henry Ginsberg: Disclosures Research
Activity 7.21 Transcription factors
Purpose To consolidate understanding of protein synthesis. To explain the role of transcription factors and hormones in switching genes on and off. Play the transcription initiation complex game Regulation
TITLE: Treatment of Prostate Cancer with a DBP-MAF-Vitamin D Complex to Target Angiogenesis and Tumorigenesis
AD AWARD NUMBER: W81XWH-04-1-0010 TITLE: Treatment of Prostate Cancer with a DBP-MAF-Vitamin D Complex to Target Angiogenesis and Tumorigenesis PRINCIPAL INVESTIGATOR: Michael W. Fannon, Ph.D. CONTRACTING
SUPPLEMENTARY DATA 1
SUPPLEMENTARY DATA 1 Supplementary Figure S1. Overexpression of untagged Ago2 inhibits the nuclear transport of the dotted foci of GFP-signal of myc-gfp-tnrc6a-nes-mut. (A C) HeLa cells expressing myc-gfp-tnrc6a-nes-mut
Primetime for KNIME:
Primetime for KNIME: Towards an Integrated Analysis and Visualization Environment for RNAi Screening Data F. Oliver Gathmann, Ph. D. Director IT, Cenix BioScience Presentation for: KNIME User Group Meeting
Protein-responsive ribozyme switches in eukaryotic cells
Protein-responsive ribozyme switches in eukaryotic cells Andrew B. Kennedy, James V. Vowles, Leo d Espaux, and Christina D. Smolke Presented by Marianne Linz and Jennifer Thornton March 11, 2015 Synthetic
A role of microrna in the regulation of telomerase? Yuan Ming Yeh, Pei Rong Huang, and Tzu Chien V. Wang
A role of microrna in the regulation of telomerase? Yuan Ming Yeh, Pei Rong Huang, and Tzu Chien V. Wang Department of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
13.4 Gene Regulation and Expression
13.4 Gene Regulation and Expression Lesson Objectives Describe gene regulation in prokaryotes. Explain how most eukaryotic genes are regulated. Relate gene regulation to development in multicellular organisms.
Expression of CD163 on Bovine Alveolar Macrophages and Peripheral Blood Mononuclear Cells
Expression of CD163 on Bovine Alveolar Macrophages and Peripheral Blood Mononuclear Cells Mary E. Kopechek Honor s Research Thesis May 17, 2007 Research Advisor: Dr. Jeff Lakritz, DVM, PhD The Ohio State
Proteins. Protein Trivia. Optimizing electrophoresis
Proteins ELECTROPHORESIS Separation of a charged particle in an electric field Michael A. Pesce, Ph.D Department of Pathology New York-Presbyterian Hospital Columbia University Medical Center Rate of migration
How To Understand How Gene Expression Is Regulated
What makes cells different from each other? How do cells respond to information from environment? Regulation of: - Transcription - prokaryotes - eukaryotes - mrna splicing - mrna localisation and translation
Give a NOD to diabetes:
Give a NOD to diabetes: NOD proteins ti link immunity it and metabolism tbli Jonathan Schertzer McMaster University McMaster University Faculty of Health Sciences Department of Biochemistry and Biomedical
Molecular Genetics. RNA, Transcription, & Protein Synthesis
Molecular Genetics RNA, Transcription, & Protein Synthesis Section 1 RNA AND TRANSCRIPTION Objectives Describe the primary functions of RNA Identify how RNA differs from DNA Describe the structure and
LEUKEMIA LYMPHOMA MYELOMA Advances in Clinical Trials
LEUKEMIA LYMPHOMA MYELOMA Advances in Clinical Trials OUR FOCUS ABOUT emerging treatments Presentation for: Judith E. Karp, MD Advancements for Acute Myelogenous Leukemia Supported by an unrestricted educational