Postdoctoral Fellow at Centre for Biotechnology and Bioengineering, Universidad de Chile

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1 Javier Gimpel Postdoctoral Fellow at Centre for Biotechnology and Bioengineering, Universidad de Chile Summary PhD from the Mayfield Laboratory at the University of California, San Diego and the California Center for Algae Biotechnology. Expertise in complex genetic engineering of the chloroplast and nuclear genomes of microalgae for expressing recombinant therapeutic proteins, altering photosynthesis and improving biofuel production. Research field expanded further into photosynthesis molecular biology and biophysics by successful collaborations with the Dismukes Laboratory (Rutgers University, NJ) and the Kramer Laboratory (Michigan State University). PostDoc at Universidad Catolica de Valparaiso, Chile, while working in genetic engineering of commercial strains of microalgae. Currently a PostDoc at the Centre for Biotechnology and Bioengineering, Universidad de Chile. Ongoing research on macroalgae biomass processing with recombinant enzymes. Experience Postdoctoral Fellow at Universidad de Chile enero de Actualidad (7 meses) Developing molecular tools for the degradation of brown macroalgae Postdoctoral Fellow at Pontificia Universidad Católica de Valparaíso julio de enero de 2015 (7 meses) Developing genetic engineering tools for commercial strains of algae (eg. Tetraselmis, Haematococcus, Nannochloropsis) Biology PhD at University of California, San Diego (4 años) Skills & Expertise Molecular Biology Genetic Engineering Algae Photosynthesis Biofuels Protein Expression Protein Purification Microbiology Page1

2 Publications Biofuels from algae: challenges and potential Biofuels 2010 Autores: Javier Gimpel, Steve Mayfield, Michael Hannon Hannon, M., J. Gimpel, M. Tran, B. Rasala, and S. Mayfield Biofuels from algae: challenges and potential. Biofuels. 1: CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES) Journal of Phycology 2012 Autores: Javier Gimpel, Vitalia Henríquez, Carla Gutiérrez, Sergio Marshall Gutiérrez, C.L., J. Gimpel, C. Escobar, S.H. Marshall, and V. Henríquez CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES). Journal of Phycology. 48: Analysis of heterologous regulatory and coding regions in algal chloroplasts Applied Microbiology and Biotechnology 2013 Autores: Javier Gimpel, Steve Mayfield Gimpel, J.A., and S.P. Mayfield Analysis of heterologous regulatory and coding regions in algal chloroplasts. Applied microbiology and biotechnology. 97: Natural variants of Photosystem II subunit D1 tune photochemical fitness to solar intensity Journal of Biological Chemistry 2013 Autores: Javier Gimpel, Steve Mayfield, Charles Dismukes, David Vinyard Vinyard, D.J., J. Gimpel, G.M. Ananyev, M.A. Cornejo, S.S. Golden, S.P. Mayfield, and G.C. Dismukes Natural variants of Photosystem II subunit D1 tune photochemical fitness to solar intensity. Journal of Biological Chemistry. 288: Genetic Engineering to Improve Algal Biofuels Production Algae for Biofuels and Energy 2013 Autores: Javier Gimpel, Steve Mayfield, Beth Rasala Rasala, B.A., J.A. Gimpel, M. Tran, M.J. Hannon, S.J. Miyake-Stoner, E.A. Specht, and S.P. Mayfield Genetic Engineering to Improve Algal Biofuels Production. In Algae for Biofuels and Energy. M. Borowitzka and N.R. Moheimani, editors. Springer Advances in microalgae engineering and synthetic biology applications for biofuel production Current Opinion in Chemical Biology 2013 Autores: Javier Gimpel, Steve Mayfield Gimpel, J.A., E.A. Specht, D.R. Georgianna, and S.P. Mayfield Advances in microalgae engineering and synthetic biology applications for biofuel production. Current opinion in chemical biology. 17: Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids Chloroplast Biotechnology - Methods and Protocols 2014 Autores: Javier Gimpel, Steve Mayfield, Daniel Barrera Page2

3 Barrera, D.J., J.A. Gimpel, and S.P. Mayfield Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids. In Chloroplast Biotechnology - Methods and Protocols. P. Maliga, editor. Springer-Verlag, New York. Engineered Photosystem II reaction centers optimize photochemistry vs. photoprotection at different solar intensities Journal of the American Chemical Society 18 de febrero de 2014 Autores: Javier Gimpel, Steve Mayfield, Charles Dismukes, David Vinyard Gimpel, J.A., D.J. Vinyard, G.M. Ananyev, S.P. Mayfield, and G.C. Dismukes Engineered Photosystem II reaction centers optimize photochemistry vs. photoprotection at different solar intensities. Journal of the American Chemical Society. In Press. Production of recombinant proteins in microalgae at pilot greenhouse scale Biotechnology & Bioengineering 13 de agosto de 2014 Autores: Javier Gimpel, James Hyun, Nathan Schoepp, Steve Mayfield Languages Spanish English (Competencia bilingüe o nativa) (Competencia bilingüe o nativa) Projects Developing molecular genetic tools to facilitate economic biofuel production in micro-algae 2010 to 2011 Miembros:Javier Gimpel, Steve Mayfield Development of genetic tools for engineering microalgae in order to optimize photosynthesis and lipid production for biofuels. Funded by the U.S. Air Force Office of Scientific Research (AFOSR). Consortium for Algal Biofuels Commercialization 2012 to 2013 Miembros:Javier Gimpel, Steve Mayfield Multi-institutional consortium funded by the U.S. Department of Energy (DOE). Identification of chloroplastic sequences from Haematococcus pluvialis: Construction of specie-specific expression vectors 2007 to Present Miembros:Javier Gimpel, Vitalia Henríquez Funded by the Office of Research, Pontificia Universidad Católica de Valparaíso, Chile. Marine biotechnology applied to the development of salmon oral vaccines 2008 to Present Miembros:Javier Gimpel, Vitalia Henríquez Funded by the Office of Research, Pontificia Universidad Católica de Valparaíso, Chile. Chloroplast genetic engineering: Powerful tool for optimizing astaxanthin biosynthesis in Haematococcus pluvialis July 2014 to Present Page3

4 Miembros:Javier Gimpel Funded by FONDEF, Chile Centre for Biotechnology and Bioengineering January 2014 to Present Miembros:Javier Gimpel Funded by Programa de Investigación Asociativa, CONICYT Honors and Awards PhD Fellowship "Beca Chile" CONICYT junio de 2012 Fellowship for covering the stipend and tuition while studying a PhD in a foreign country. Ranked 1st in Graduation Cohort Basic Science Division, Pontificia Universidad Católica de Valparaíso 2008 Education University of California, San Diego Doctor of Philosophy (PhD), Molecular Biology, Pontificia Universidad Católica de Valparaíso Bachelor of Science (BS), Biology/Biological Sciences, General, Grade: Biologist Page4

5 Javier Gimpel Postdoctoral Fellow at Centre for Biotechnology and Bioengineering, Universidad de Chile Contact Javier on LinkedIn Page5

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