LC-MSMS - state of the art in endocrinology

Size: px
Start display at page:

Download "LC-MSMS - state of the art in endocrinology"

Transcription

1 LC-MSMS - state of the art in endocrinology Michael Vogeser Institute of Clinical Chemistry Hospital of the University of Munich Germany 8 June 2011, Namur

2 Quantification of marker molecules is essential in the diagnostic work-up of ALL endocrinological disorders.

3 Quantification of marker molecules is essential in the diagnostic work-up of ALL endocrinological disorders. The interest of endocrinologists in high-quality analytics is essential.

4 Quantification of marker molecules is essential in the Diagnostic work-up of ALL endocrinological disorders. The interest of endocrinologists in high-quality analytics is essential. Immunoassays (routine analyses) and GC-MS (reference methods): traditional players in endocrinological analytics

5 Quantification of marker molecules is essential in the Diagnostic work-up of ALL endocrinological disorders. The interest of endocrinologists in high-quality analytics is essential. Immunoassays (routine analyses) and GC-MS (reference methods): traditional players in endocrinological analytics LC-MS/MS: In use for >10 years now

6 Potential roles of LC-MS/MS in endocrinology

7 Potential roles of LC-MS/MS in endocrinology Classical reference methods Reference measurements in large series (applying ID) Research method (known analytes / new analytes) Routine analytics

8 Potential targets of LC-MS/MS in endocrinology

9 Potential targets of LC-MS/MS in endocrinology Small molecule hormones Proteo- / peptide-hormones Endocrine tumour markers Xenobiotics (endocrinological drugs; endocrine disruptors) Metabolites / patterns of metabolic hormone effects

10 Protein quantification by mass-spectrometry

11 Protein quantification by mass-spectrometry MS has a key role in proteomics characterisation of proteins Quantification of proteins is a substantial challenge for LC-MS/MS (low concentrations, heterogeneity of analytes) First reference methods described (e.g. HbA1c, insulin) Immunoassay-MS-hybrid assays (e.g., PTH, thyreoglobulin) Potential advantage: precise characterisation of sub-forms and isoform-patterns No routine applications at present

12 Small molecule hormone quantification by LC-MS/MS

13 Small molecule hormone quantification by LC-MS/MS Potential strengths of LC-MS/MS High potential specificity > reliable standardisation possible Isotope dilution internal standardisation > compensation of matrix effects, high accuracy (in particular when compared to competitive immunoassays) Good between-laboratory agreement, assay-independent reference ranges (relevant for complex sub-populations in endocrinology) No interference from heterophilic antibodies Independence from antibody-reagent lots Cost efficiency ( biology is expensive, chemistry is cheap ) Good practicability, potentially high sample throughput ( GC-MS)

14 LC-MS/MS in endocrinology - status of application

15 LC-MS/MS in endocrinology - status of application University hospital laboratories / teaching hospitals: widely used technology; routine / research combi-use Commercial laboratories: standard technology in core labs. TDM >> endocrinology

16 LC-MS/MS in endocrinology - status of application University hospital laboratories / teaching hospitals: widely used technology; routine / research combi-use Commercial laboratories: standard technology in core labs. TDM >> endocrinology In-house methods / laboratory developed tests / home-brew Kits (Chromsystems, Recipe, Biocrates, Perkin Elmer)

17 LC-MS/MS in endocrinology - status of application University hospital laboratories / teaching hospitals: widely used technology; routine / research combi-use Commercial laboratories: standard technology in core labs. TDM >> endocrinology In-house methods / laboratory developed tests / home-brew Kits (Chromsystems, Recipe, Biocrates, Perkin Elmer) Implementation of the technology, method development, method maintenance is a substantial challenge! Poor support by the MS-manufacturer (applications) Routine use possible but not in a standard laboratory

18 Small molecule analytes in endocrinology

19 Small molecule analytes in endocrinology 25-OH-Vitamin D3 (=hormone precursor) Plasma metanephrines Testosterone (total) Cortisol (urine (saliva, serum)) 17-OH-Progesteron routinely analyzed in a number of laboratories by LC-MS/MS

20 Small molecule analytes in endocrinology 25-OH-Vitamin D3 (=hormone precursor) Plasma metanephrines Testosterone (total) Cortisol (urine (saliva, serum)) 17-OH-Progesteron Steroid profiles (Cortisol, cortison, 11-deoxycortisol, 21-deoxycortisol, corticosterone, 17-OH-P, androstendion, testosterone, progesteron, DHEA, DHEAS) routinely analyzed in a number of laboratories by LC-MS/MS analyzed in some routine laboratories Urinary 5-HIA / serotonin

21 Small molecule analytes in endocrinology 25-OH-Vitamin D3 (=hormone precursor) Plasma metanephrines Testosterone (total) Cortisol (urine (saliva, serum)) 17-OH-Progesteron Steroid profiles (Cortisol, cortison, 11-deoxycortisol, 21-deoxycortisol, corticosterone, 17-OH-P, androstendion, testosterone, progesteron, DHEA, DHEAS) routinely analyzed in a number of laboratories by LC-MS/MS analyzed in some routine laboratories Urinary 5-HIA / serotonin Free thyroid hormones (after ED / UF) reference methods second line routine

22 Small molecule analytes in endocrinology 25-OH-Vitamin D3 (=hormone precursor) Plasma metanephrines Testosterone (total) Cortisol (urine (saliva, serum)) 17-OH-Progesteron Steroid profiles (Cortisol, cortison, 11-deoxycortisol, 21-deoxycortisol, corticosterone, 17-OH-P, androstendion, testosterone, progesteron, DHEA, DHEAS) routinely analyzed in a number of laboratories by LC-MS/MS analyzed in some routine laboratories Urinary 5-HIA / serotonin Free thyroid hormones (after ED / UF) reference methods second line routine Aldosterone challenge! 1,25-dihydroxy vitamin D research 17-ß-Estradiol / estrogens, progesterone / gestagens

23 Small molecule analytes in endocrinology Analytes related to research :

24 Small molecule analytes in endocrinology Analytes related to research : e.g. Prostaglandins Endocannabinoids Endogenous digitalis-like substances Tamoxifen Bisphenol-A Metabolomics of stress-response

25 Problems and sources of errors

26 Problems and sources of errors Practicability full commitment of experts required on site (method implementation, handling of problems) Gross handling errors (e.g. errors in labelling, data transfer to LIMS) Ion suppression and matrix effects on ionisation stable isotope labelled internal standards available Appropriate calibration materials ( kits) Sensitivity (cortisol vs. aldosteron!) Isomers / isobars of the target analytes ( chromatographic separation)

27 Isobaric interferences 11-Deoxy X 21-Deoxy X X 17-Deoxy 3-epi 25-OH-vitamin D3 epi-testosterone 2-AG / 1-AG structurally unrelated isobars? sufficient degree of chromatography is essential! Dr. Seger, Innsbruck 2009

28 Two examples of application of LC-MS/MS in endocrinology

29 25-OH vitamin D Kritik an der HPLC Messung von 25-OH Vitamin D mit IDS-RIA Ligandenbindungs-Assays IDS-EIA Nichols Liaison 1 Liaison 2 Roche Bias-Plot (y-achse LC-MS/MS) Roth et al., Ann Clin Biochem 2008;45:153-9 large scale validation of routine methods by LC-MS/MS

30 Legro SR et al. J Clin Endocin Metabol 2010;95: Blinded send-out of paired samples to an academic laboratory and to a commercial laboratory for testosterone quantification by LC-MS/MS and RIA Sub-set of paired samples in the same laboratory correlation and reproducibility?

31 Testosterone by LC-MS/MS

32 Testosterone by LC-MS/MS

33 Future perspectives of LC-MS/MS in endocrinology

34 Future perspectives of LC-MS/MS in endocrinology Probable: Widespread application of good kit solutions Automation of sample preparation Better standardisation of instrument configurations and handling Increasing percentage of MS-analyses in endocrine testing

35 Future perspectives of LC-MS/MS in endocrinology Probable: Widespread application of good kit solutions Automation of sample preparation Better standardisation of instrument configurations and handling Increasing percentage of MS-analyses in endocrine testing Desirable but uncertain: Development of plug-and-play closed MS/MS-based analyzers by the IVD-industry Transformation from a routine technology in specialised laboratories to a routine technology for standard endocinology labs (as realized for immunoassays) Metabolomic profiling approaches to endocrinological investigations

36 Σ LC-MS/MS in endocrinology:

37 LC-MS/MS in endocrinology: powerful technology Σ

38 LC-MS/MS in endocrinology: powerful technology demanding technology Σ

39 Σ LC-MS/MS in endocrinology: powerful technology demanding technology technology under development

40 LC-MS/MS in endocrinology: powerful technology demanding technology Σ technology under development routine technology in many laboratories but has still a long way to a widespread standard technology

41 LC-MS/MS in endocrinology: powerful technology demanding technology Σ technology under development routine technology in many laboratories but has still a long way to a widespread standard technology probably has the potential to improve clinical care by analyses of superior quality compared to standard techniques

42

43 Hospital of the University of Munich ~ 2,300 beds ~ 9,900 co-workers ~ 5,000 students 2 main locations: Campus Grosshadern and Campus Inner City Institute of Clinical Chemistry ~ 200 co-workers 4 affiliates ~ 8 mio. analyses per years Clinical chemistry, haematology, coagulation, immunology and endocrinology testing, therapeutic drug monitoring, human genetics

44 Mass spectrometry in the Institute of Clinical Chemistry Introduction of GC-MS in 1975 (urinary catecholamine metabolites, organic acids, stable isotope metabolic-turn-over studies, etc.) Introduction of LC-MS/MS in 2000 At present analysis of tacrolimus, cyclosporin, everolimus, sirolimus, busulfan, itraconazole, posaconazole; research applications (e.g., steroids) ~ 200 samples per day 4 instruments (Waters) 10 technicians trained

UPLC/MSMS in the analysis of physiological steroids. May 2012. Anders Feldthus. Anders_Feldthus@Waters.com. 2012 Waters Corporation 1

UPLC/MSMS in the analysis of physiological steroids. May 2012. Anders Feldthus. Anders_Feldthus@Waters.com. 2012 Waters Corporation 1 UPLC/MSMS in the analysis of physiological steroids Anders Feldthus Anders_Feldthus@Waters.com May 2012 2012 Waters Corporation 1 Clinical Chemistry Mass Spectrometry Citations (1955-2006) 70 Number of

More information

Sex Hormone Testing by Mass Spectrometry

Sex Hormone Testing by Mass Spectrometry Sex Hormone Testing by Mass Spectrometry Robert L. Fitzgerald, PhD, DABCC Professor of Pathology University of California-San Diego San Diego, CA, 92161 rfitzgerald@ucsd.edu Learning Objectives After this

More information

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs Thermo Scientific Prelude SPLC System FPO Making LC/MS accessible to clinical research and toxicology labs Specifically designed to make the power of LC/MS accessible Analysis of target compounds in complex

More information

HRMS in Clinical Research: from Targeted Quantification to Metabolomics

HRMS in Clinical Research: from Targeted Quantification to Metabolomics A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier

More information

Immunoassay. Product Portfolio. www.diametra.com info@diametra.com Part of the IDS group

Immunoassay. Product Portfolio. www.diametra.com info@diametra.com Part of the IDS group Immunoassay Portfolio www.diametra.com info@diametra.com Part of the IDS group Bringing Value-Added Efficiency To Your Laboratory Diametra is dedicated to providing quality products and the highest level

More information

Advances in Steroid Panel Analysis with High Sensitivity LC/MS/MS. Kenneth C. Lewis 1, Lisa St. John-Williams 1, Changtong Hao 2, Sha Joshua Ye 2

Advances in Steroid Panel Analysis with High Sensitivity LC/MS/MS. Kenneth C. Lewis 1, Lisa St. John-Williams 1, Changtong Hao 2, Sha Joshua Ye 2 Advances in Steroid Panel Analysis with High Sensitivity LC/MS/MS Kenneth C. Lewis 1, Lisa St. John-Williams 1, Changtong Hao 2, Sha Joshua Ye 2 1 OpAns LLC, Durham, NC, USA; 2 Ionics Mass Spectrometry

More information

The Measurement of Vitamin D

The Measurement of Vitamin D The Measurement of Vitamin D Solving a challenging assay in the clinical research laboratory 2008 Waters Corporation Outline Introduction to Vitamin D Automated SPE approach to Vit D analysis 2008 Waters

More information

MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification

MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification Gold Standard for Quantitative Data Processing Because of the sensitivity, selectivity, speed and throughput at which MRM assays can

More information

The Facts on Equine Drug Testing

The Facts on Equine Drug Testing The Facts on Equine Drug Testing CONTACT Travis Mays, MS Interim Section Head Analytical Chemistry, Drug Testing Laboratory Toxicology Laboratory 979.845.3414 tmays@ LIMITATIONS IN DRUG TESTING While advancements

More information

Sensitive steroid analysis on a new High Speed Triple Quadrupole LC/MS/MS. Mikaël LEVI Shimadzu France

Sensitive steroid analysis on a new High Speed Triple Quadrupole LC/MS/MS. Mikaël LEVI Shimadzu France Sensitive steroid analysis on a new High Speed Triple Quadrupole LC/MS/MS Mikaël LEVI Shimadzu France Welcome Seminar Barcelona 2013 Interest in Steroids assay by LC-MS/MS Steroids measurement is essential

More information

Future of Mass Spectrometry in Clinical Research and Forensic Toxicology. Brad Hart Market Development Director

Future of Mass Spectrometry in Clinical Research and Forensic Toxicology. Brad Hart Market Development Director Future of Mass Spectrometry in Clinical Research and Forensic Toxicology Brad Hart Market Development Director Agenda The Heritage of Mass Spec vendors Market Conditions Regulatory Challenges Trends/ Frontiers

More information

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research UPLC-MS/MS Analysis of in Plasma for Clinical Research Dominic Foley and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity improves reproducibility through removal

More information

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay PO-CON1484E Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching ASMS 014 TP85 David R. Baker 1, John Warrander 1, Neil Loftus 1, Simon Hird

More information

Calcium. Table 1: Difference between method means in percent

Calcium. Table 1: Difference between method means in percent Calcium Measurement of total calcium is widely used for both the diagnosis and the monitoring of a range of conditions related to the bones, heart, nerves, and kidneys. Total calcium measurements include

More information

NUVISAN Pharma Services

NUVISAN Pharma Services NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!

More information

Factors Influencing LC/MS/MS Moving into Clinical and Research Laboratories

Factors Influencing LC/MS/MS Moving into Clinical and Research Laboratories Factors Influencing LC/MS/MS Moving into Clinical and Research Laboratories Matthew Clabaugh, Market Development AACC Workshop St. Louis, MO September 17,18 2013 Factors Influencing LCMSMS Moving into

More information

AbsoluteIDQ p180 Kit. Targeted Metabolite Identifi cation and Quantifi cation. Bringing our targeted metabolomics expertise to your lab.

AbsoluteIDQ p180 Kit. Targeted Metabolite Identifi cation and Quantifi cation. Bringing our targeted metabolomics expertise to your lab. AbsoluteIDQ p180 Kit Targeted Metabolite Identifi cation and Quantifi cation Bringing our targeted metabolomics expertise to your lab. The Biocrates AbsoluteIDQ p180 mass spectrometry Assay Preparation

More information

Quan / Qual Analyses in Clinical Research using a Second Generation Exactive High Resolution Mass Spectrometer

Quan / Qual Analyses in Clinical Research using a Second Generation Exactive High Resolution Mass Spectrometer Quan / Qual Analyses in Clinical Research using a Second Generation Exactive High Resolution Mass Spectrometer Bertrand Rochat, PhD quantitative Mass Spectrometry Facility Lake of Geneva, Lausanne, Switzerland

More information

LC-MS/MS for Chromatographers

LC-MS/MS for Chromatographers LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,

More information

Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances

Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances TripleTOF 5600 + LC/MS/MS System with MasterView Software Adrian M. Taylor AB Sciex Concord, Ontario (Canada) Overview

More information

IMMUNOASSAY CONTROL - LEVEL 1 (IA CONTROL 1)

IMMUNOASSAY CONTROL - LEVEL 1 (IA CONTROL 1) 0843 IMMUNOASSAY CONTROL - LEVEL 1 (IA CONTROL 1) Cat. No. IA2638 / IA2633 Lot No. 978EC Size: 10 x 5 ml / 12 x 5 ml Expiry: 2013-10 INTENDED USE This product is intended for in vitro diagnostic use in

More information

Multiple technologies

Multiple technologies Thermo Scientific CEDIA, DRI and QMS Technologies One resource Multiple technologies Drugs of Abuse Oral Fluids Drugs of Abuse Urine/Serum Endocrine Immunosuppressant Drug Monitoring Specimen Validity

More information

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC Katja SÄRKKÄ, Kari ARINIEMI, Pirjo LILLSUNDE Laboratory of Substance Abuse, National Public Health Institute Manerheimintie,

More information

DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited.

DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Investigation of the Physiological Responses of Belugas to Stressors to Aid in Assessing the Impact of Environmental and

More information

GUIDELINES ON USE OF URINARY STEROID PROFILING DURING OR AFTER GLUCOCORTICOID TREATMENT AND IN CONJUNCTION WITH SUPPRESSION AND STIMULATION TESTING

GUIDELINES ON USE OF URINARY STEROID PROFILING DURING OR AFTER GLUCOCORTICOID TREATMENT AND IN CONJUNCTION WITH SUPPRESSION AND STIMULATION TESTING GUIDELINES ON USE OF URINARY STEROID PROFILING DURING OR AFTER GLUCOCORTICOID TREATMENT AND IN CONJUNCTION WITH SUPPRESSION AND STIMULATION TESTING Background ACTH acts on the adrenal cortex in a short

More information

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz Toxichem Krimtech 211;78(Special Issue):324 Online extraction LC-MS n method for the detection of drugs in urine, serum and heparinized plasma Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische

More information

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS Summary: Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS UCT Part Numbers: CUNAX226 - Clean-Up C8+NAX, 2mg/6mL BETA-GLUC- ml Beta-Glucuronidase Enzyme, liquid form SLAQ1ID21-3UM -

More information

What Do We Learn about Hepatotoxicity Using Coumarin-Treated Rat Model?

What Do We Learn about Hepatotoxicity Using Coumarin-Treated Rat Model? What Do We Learn about Hepatotoxicity Using Coumarin-Treated Rat Model? authors M. David Ho 1, Bob Xiong 1, S. Stellar 2, J. Proctor 2, J. Silva 2, H.K. Lim 2, Patrick Bennett 1, and Lily Li 1 Tandem Labs,

More information

HA Convention 2007 Service Priorities & Programmes 8

HA Convention 2007 Service Priorities & Programmes 8 HA Convention 2007 Service Priorities & Programmes 8 An Expenditure-saving Cluster-wide Cyclosporin A Service with Improved Analytical Performance Dr. Michael H. M. Chan MBChB FRCPA FHKCPath FHKAM(Pathology)

More information

Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem

Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Kevin Van Cott, Associate Professor Dept. of Chemical and Biomolecular Engineering Nebraska

More information

ACTIVE-B12 EIA. the next level of B12 testing

ACTIVE-B12 EIA. the next level of B12 testing ACTIVE-B12 EIA the next level of B12 testing Vitamin B12 an essential nutrient Vitamin B12 is an essential nutrient (can only be obtained from the diet) and is a vital component in many cellular functions

More information

Common Endocrine Disorders. Gary L. Horowitz, MD Beth Israel Deaconess Medical Center Boston, MA

Common Endocrine Disorders. Gary L. Horowitz, MD Beth Israel Deaconess Medical Center Boston, MA Common Endocrine Disorders Gary L. Horowitz, MD Beth Israel Deaconess Medical Center Boston, MA Objectives Describe the typical laboratory values for TSH and Free T4 in hypo- and hyperthyroidism Explain

More information

Thyroglobulin. versie 071120 J. Billen LAG-UZ-KULeuven 1

Thyroglobulin. versie 071120 J. Billen LAG-UZ-KULeuven 1 Thyroglobulin Large glycoprotein Two identical polypeptide chains 660 kda Prohormone in the intra-thyroid T4 and T3 synthesis Produced only by normal thyrocytes or well-differentiated thyroid cancer (DTC)

More information

ARTICLE IN PRESS Steroids xxx (2010) xxx xxx

ARTICLE IN PRESS Steroids xxx (2010) xxx xxx Steroids xxx (2010) xxx xxx Contents lists available at ScienceDirect Steroids journal homepage: www.elsevier.com/locate/steroids Review Measurement of 25-hydroxyvitamin D in the clinical laboratory: Current

More information

Blood Testing Protocols. Disclaimer

Blood Testing Protocols. Disclaimer Blood Testing Protocols / Page 2 Blood Testing Protocols Here are the specific test protocols recommend by Dr. J.E. Williams. You may request these from your doctor or visit www.readyourbloodtest.com to

More information

Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction

Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction Drug testing beyond the health care and criminal justice systems has

More information

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging

More information

Building innovative drug discovery alliances. Evotec Munich. Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs

Building innovative drug discovery alliances. Evotec Munich. Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs Building innovative drug discovery alliances Evotec Munich Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs Evotec AG, Evotec Munich, June 2013 About Evotec Munich A leader

More information

泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics

泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 2014 Training Course Wei-Hung Chang ( 張 瑋 宏 ) ABRC, Academia

More information

Chapter 45: Hormones and the Endocrine System

Chapter 45: Hormones and the Endocrine System Name Period Overview 1. What is a hormone? 2. Why does a hormone elicit a response only with target cells? 3. The body has two long-distance regulating systems. Which involves chemical signals by hormones?

More information

aurora ICP-MS Innovation with Integrity The Science of Sensitivity ICP-MS

aurora ICP-MS Innovation with Integrity The Science of Sensitivity ICP-MS aurora ICP-MS The Science of Sensitivity Innovation with Integrity ICP-MS Powers the Business of Quantification aurora ICP-MS enhanced value package Bruker s ICP-MS is famous for its innovative and unique

More information

PPD LABORATORIES CENTRAL LAB: SUPERIOR SERVICE, QUALITY DATA WITHOUT COMPROMISES

PPD LABORATORIES CENTRAL LAB: SUPERIOR SERVICE, QUALITY DATA WITHOUT COMPROMISES PPD LABORATORIES CENTRAL LAB: SUPERIOR SERVICE, QUALITY DATA WITHOUT COMPROMISES PPD Laboratories provides world-class scientific expertise with state-of-the-art technologies supported by a commitment

More information

Cliquid ChemoView 3.0 Software Simple automated analysis, from sample to report

Cliquid ChemoView 3.0 Software Simple automated analysis, from sample to report PRODUCT BULLETIN Cliquid ChemoView 3.0 Software for Routine Screening and Quantitation Cliquid ChemoView 3.0 Software Simple automated analysis, from sample to report KEY FEATURES Secure user login that

More information

Vitamin B 12 - A review of analytical methods for use in food. Government Chemist Programme Report

Vitamin B 12 - A review of analytical methods for use in food. Government Chemist Programme Report Vitamin B 12 - A review of analytical methods for use in food Government Chemist Programme Report March 2015 Vitamin B12 - A review of analytical methods for use in food Author: Paul Lawrance Contact point:

More information

Course Curriculum for Master Degree in Medical Laboratory Sciences/Clinical Biochemistry

Course Curriculum for Master Degree in Medical Laboratory Sciences/Clinical Biochemistry Course Curriculum for Master Degree in Medical Laboratory Sciences/Clinical Biochemistry The Master Degree in Medical Laboratory Sciences /Clinical Biochemistry, is awarded by the Faculty of Graduate Studies

More information

ST. VINCENT'S. MEDICAL CENTER St. Vincent's Healthcare

ST. VINCENT'S. MEDICAL CENTER St. Vincent's Healthcare ST. VINCENT'S MEDICAL CENTER St. Vincent's Healthcare Medical Technology St. Vincent s Schools of Medical Science Throughout Northeast Florida and Southern Georgia, St. Vincent s HealthCare is well known

More information

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS Therapeutic Drug Monitoring of Antiretroviral Drugs with PLC-M Ursula Gutteck-Amsler, Katharina M. Rentsch Abstract Prospective and retrospective studies have provided some evidence of the clinical and

More information

BIOMARKERS AND TOXICITY MECHANISMS 06 Mechanisms Metabolism & Detoxification. Luděk Bláha, PřF MU, RECETOX www.recetox.cz

BIOMARKERS AND TOXICITY MECHANISMS 06 Mechanisms Metabolism & Detoxification. Luděk Bláha, PřF MU, RECETOX www.recetox.cz BIOMARKERS AND TOXICITY MECHANISMS 06 Mechanisms Metabolism & Detoxification Luděk Bláha, PřF MU, RECETOX www.recetox.cz Metabolism and detoxification Chemicals enter body... mostly via food Pass directly

More information

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP and TripleTOF 5600 System Technology Targeted peptide quantification is a rapidly growing application

More information

A Second-Tier Screening Approach for CAH: Oklahoma s Experience

A Second-Tier Screening Approach for CAH: Oklahoma s Experience A Second-Tier Screening Approach for CAH: Oklahoma s Experience Kathy Kirk, RN, MSN Theresa Steckel, BSN, RN Pam King, MPA, RN Debbie Kline, BS Edd Rhoades, MD, MPH Kenneth Copeland, MD U.S. Newborn Screening

More information

Monoclonal Antibodies, Brochure

Monoclonal Antibodies, Brochure Monoclonal Antibodies, Brochure Interest in any of the products, request or order them at Bio-Connect Diagnostics. Bio-Connect Diagnostics B.V. T NL +31 (0)26 326 44 60 T BE +32 (0)2 502 12 53 Begonialaan

More information

What Does Having a FDA Cleared Pregnancy Test Mean?

What Does Having a FDA Cleared Pregnancy Test Mean? What Does Having a FDA Cleared Pregnancy Test Mean? Clinical Trials Transformation Initiative (CTTI) July 15-16, 2013 Denise N. Johnson-Lyles, Ph.D. Toxicology Branch Chief Division of Chemistry and Toxicology

More information

Analytical Testing Methods

Analytical Testing Methods Analytical Testing Methods Updated: February 2005 Background The Mandatory Guidelines for Federal Workplace Drug Testing Programs require a laboratory to conduct two analytical tests before a urine specimen

More information

The sensitive marker for glomerular filtration rate (GFR) Estimation of GFR from Serum Cystatin C:

The sensitive marker for glomerular filtration rate (GFR) Estimation of GFR from Serum Cystatin C: The sensitive marker for glomerular filtration rate (GFR) Estimation of GFR from Serum Cystatin C: The good correlation allows close estimation of GFR Cystatin C GFR GFR in serum estimated* measured* n

More information

Guideline on bioanalytical method validation

Guideline on bioanalytical method validation 21 July 2011 EMEA/CHMP/EWP/192217/2009 Rev. 1 Corr. 2** Committee for Medicinal Products for Human Use (CHMP) Draft agreed by the Efficacy Working Party September 2009 Adoption by CHMP for release for

More information

Definition of the Measurand: CRP

Definition of the Measurand: CRP A Reference Measurement System for C-reactive Protein David M. Bunk, Ph.D. Chemical Science and Technology Laboratory National Institute of Standards and Technology Definition of the Measurand: Human C-reactive

More information

Rapid and Reproducible Amino Acid Analysis of Physiological Fluids for Clinical Research Using LC/MS/MS with the atraq Kit

Rapid and Reproducible Amino Acid Analysis of Physiological Fluids for Clinical Research Using LC/MS/MS with the atraq Kit Rapid and Reproducible Amino Acid Analysis of Physiological Fluids for Clinical Research Using LC/MS/MS with the atraq Kit Fast, simple and cost effective analysis Many areas of biochemical research and

More information

DMPK: Experimentation & Data

DMPK: Experimentation & Data DMPK: Experimentation & Data Interpretation Mingshe Zhu, Mike S. Lee, Naidong Weng, and Mark Hayward Prerequisite: Entry-level scientists with hands on experience in LC/MS as well as advanced students

More information

Introduction to mass spectrometry (MS) based proteomics and metabolomics

Introduction to mass spectrometry (MS) based proteomics and metabolomics Introduction to mass spectrometry (MS) based proteomics and metabolomics Tianwei Yu Department of Biostatistics and Bioinformatics Rollins School of Public Health Emory University September 10, 2015 Background

More information

IMMUNOASSAY PREMIUM PLUS LEVEL 3 (IA PREMIUM PLUS 3)

IMMUNOASSAY PREMIUM PLUS LEVEL 3 (IA PREMIUM PLUS 3) 0843 IMMUNOASSAY PREMIUM PLUS LEVEL 3 (IA PREMIUM PLUS 3) Cat. No.: IA3111 / IA3112 Lot No.: 1130EC Size.: 12 x 5ml / 12 x 5ml Expiry.: 2014-10 INTENDED USE This product is intended for in vitro diagnostic

More information

POLYTECHNIC OF NAMIBIA

POLYTECHNIC OF NAMIBIA POLYTECHNIC OF NAMIBIA DEPARTMENT OF HEALTH SCIENCES BACHELOR OF BIOMEDICAL SCIENCES Clinical chemistry 1 (CLC 310 S) SECOND OPPORTUNITY EXAMINATION NOVEMBER 2014 LEVEL: TIME: MARKS: PAPER: PAGES: 7 3

More information

Free Testosterone Cat# 2924Z

Free Testosterone Cat# 2924Z See external label 2 C-8 C Σ=96 tests Cat # 2924Z Free Testosterone Cat# 2924Z Direct immunoenzymatic determination of Free Testosterone in serum or plasma. For in vitro diagnostic use only INTENDED USE

More information

Vitamin D toxicity? A case study

Vitamin D toxicity? A case study Vitamin D toxicity? A case study Vichet Khieng, BMLSc, Medical Laboratory Scientist; Catherine Stevens, NZCS, Senior Medical Laboratory Scientist Department of Biochemistry, Southern Community Laboratories,

More information

Thyroid Stimulating Hormone. 3rd Generation Ultra Sensitive Assay

Thyroid Stimulating Hormone. 3rd Generation Ultra Sensitive Assay Thyroid Stimulating Hormone 3rd Generation Ultra Sensitive Assay Table of Contents 2 Introduction 3 TSH Materials and Methods 4 Results and Interpretation 5 Conclusion Notes Introduction Thyroid function

More information

6. ANALYTICAL METHODS

6. ANALYTICAL METHODS 45 6.1 BIOLOGICAL MATERIALS No methods were located that are routinely used for the detection of BCEE in biological materials. Norpoth et al. (1986) reported a method for measuring thiodiglycolic acid

More information

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring

The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,

More information

Bruker ToxScreener TM. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS

Bruker ToxScreener TM. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS Bruker ToxScreener TM A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integrity UHR-TOF MS ToxScreener - Get the Complete Picture Forensic laboratories are frequently required

More information

Central Government Laboratory Department of Health and Environment

Central Government Laboratory Department of Health and Environment Central Government Laboratory Department of Health and Environment Overview who we are and what we do What is available and what is protocol for good science Sample collection Screening Tests Confirmation

More information

P R O D U C T S CATALOG 2011-2012

P R O D U C T S CATALOG 2011-2012 P R O D U C T S CATALOG 2011-2012 INSTITUTE FOR THE APPLICATION OF NUCLEAR ENERGY - INEP Banatska 31b 11080 Zemun Belgrade Serbia Tel: (+381 11) 2619 252, 2618 696, 2199 949 Fax: (+381 11) 2618 724 www.inep.co.rs

More information

INTRODUCTION TO HORMONES

INTRODUCTION TO HORMONES INTRODUCTION TO HORMONES UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DISCIPLINE OF BIOCHEMISTRY & MOLECULAR BIOLOGY PBL MBBS II SEMINAR VJ Temple What are hormones? Cells in multi-cellular

More information

Veterinary Testing. Classes of Test

Veterinary Testing. Classes of Test Veterinary Testing Classes of Test July 2014 Copyright National Association of Testing Authorities, Australia 2014 This publication is protected by copyright under the Commonwealth of Australia Copyright

More information

Michael L. DeVan, 1 Daniel D. Bankson, 2 and Jude M. Abadie 1. Abstract

Michael L. DeVan, 1 Daniel D. Bankson, 2 and Jude M. Abadie 1. Abstract Clinical Chemistry / Measurable Free Testosterone Levels To What Extent Are Free Testosterone (FT) Values Reproducible Between the Two Washingtons, and Can Calculated FT Be Used in Lieu of Expensive Direct

More information

Development and Validation of a LC-MS/MS Method for Plasma Analysis of the Serotonin Metabolite: 5-Hydroxyindoleacetic acid (5-HIAA)

Development and Validation of a LC-MS/MS Method for Plasma Analysis of the Serotonin Metabolite: 5-Hydroxyindoleacetic acid (5-HIAA) W Ward, B Begley, R Friley, M Barringer,, J Nofsinger, M Allen, D Hudson, D Witt Tandem Labs, Laboratory Corporation of America Holdings P Rosner, A Wilson, S Wason,, J Townsend, L Law, QM Yang Lexicon

More information

Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software

Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Abstract Presented here is a case study of a walk-up liquid chromatography

More information

Quantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry

Quantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry Quantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry Paula Ladwig, David Barnidge, Mindy Kohlhagen, John Mills, Maria Willrich, Melissa R. Snyder and David Murray

More information

Trends and Challenges in Therapeutic Drug Monitoring: Is LC/MS the Solution?

Trends and Challenges in Therapeutic Drug Monitoring: Is LC/MS the Solution? Mass Spectrometry in the Clinical Lab: Best Practices and Current Applications September 6-7, 2012 Chicago, IL Trends and Challenges in Therapeutic Drug Monitoring: Is LC/MS the Solution? Paul J. Jannetto,

More information

Advances in LC-MS bioanalysis for drug research and development 液 相 质 谱 生 物 样 品 分 析 技 术 在 新 药 研 发 中 的 新 进 展

Advances in LC-MS bioanalysis for drug research and development 液 相 质 谱 生 物 样 品 分 析 技 术 在 新 药 研 发 中 的 新 进 展 Advances in LC-MS bioanalysis for drug research and development 液 相 质 谱 生 物 样 品 分 析 技 术 在 新 药 研 发 中 的 新 进 展 张 杰 DMPK, Novartis Institutes for BioMedical Research ( 诺 华 ), New Jersey 2014 南 京 国 际 药 代 会

More information

Guidance for Industry

Guidance for Industry Guidance for Industry Bioanalytical Method Validation U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine

More information

A Cell Culture Based Assay for the Measurement of Xenobiotic Effects on Steroid Production

A Cell Culture Based Assay for the Measurement of Xenobiotic Effects on Steroid Production A Cell Culture Based Assay for the Measurement of Xenobiotic Effects on Steroid Production P.D. Jones, M. Hecker, T. Gracia, X. Zhang, J. T. Sanderson*, J. P. Giesy, J. L. Newsted Aquatic Toxicology Laboratory,

More information

MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples

MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples Fast and Efficient Data Review, with Automatic Flagging of Outlier Results Adrian M. Taylor and Michael

More information

WATERS QUANTITATIVE ANALYSIS solutions

WATERS QUANTITATIVE ANALYSIS solutions More sensitivity. More speed. What more can you ask for in a complete quantitative analysis solution? WATERS QUANTITATIVE ANALYSIS solutions THE CHALLENGE OF QUANTITATIVE ANALYSIS Tandem mass spectrometry,

More information

Mass Spectrometry Signal Calibration for Protein Quantitation

Mass Spectrometry Signal Calibration for Protein Quantitation Cambridge Isotope Laboratories, Inc. www.isotope.com Proteomics Mass Spectrometry Signal Calibration for Protein Quantitation Michael J. MacCoss, PhD Associate Professor of Genome Sciences University of

More information

Urine Drug Testing. Why drug test? Set the Standard!

Urine Drug Testing. Why drug test? Set the Standard! Urine Drug Testing Urine drug testing is a valuable tool for the physician when making treatment decisions. Patients and physicians benefit from safe and effective treatment of pain but there are always

More information

Graham Beastall. gbeastall@googlemail.com. Outline of Talk

Graham Beastall. gbeastall@googlemail.com. Outline of Talk Harmonization of Methods in Laboratory Medicine: A Means to Improve Patient Safety Graham Beastall gbeastall@googlemail.com Outline of Talk Introduction Why Standardize or Harmonize Methods? Traceability

More information

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Jeff Layne, Michael McCoy, and Sky Countryman Phenomenex, Inc., 411 Madrid Ave.,

More information

Calcium Metabolism physiology of hormone 2007

Calcium Metabolism physiology of hormone 2007 Calcium Metabolism physiology of hormone 2007 Calcium metabolism What is the recommended daily intake? 1000mg What is the plasma concentration? 2.2-2.6mmol/L 2.6mmol/L How is calcium excreted? Kidneys

More information

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1

GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1 GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert, Michel Lhermitte, Frederic Grisel Laboratoire de Toxicologie & Génopathologie, CHRU Lille, France Waters Corporation, Guyancourt,

More information

Pseudohypoparathyroidism: A Variation on the Theme of Hypoparathyroidism

Pseudohypoparathyroidism: A Variation on the Theme of Hypoparathyroidism Pseudohypoparathyroidism: A Variation on the Theme of Hypoparathyroidism Amanda Tencza MS IV 1 and Michael A. Levine, MD 2 1 Cleveland Clinic Lerner College of Medicine of Case Western Reserve University

More information

Introduction. Methods. Sample Processing

Introduction. Methods. Sample Processing PO-CON1656 High-sensitivity, high-throughput quantitation of catecholamines and metanephrine in plasma by automated WCX-SP coupled to LC/MS/MS for clinical research ASMS 2016 MP-080 Ichiro HIRANO 1, Atsuhiko

More information

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi-

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi- 11 Cryo 5 Tubes/Multi 2 HTS 3 Immunology / Immunology Technical Information 3 I 2 96 Well ELISA 3 I 4 96 Well ELISA Strip Plates 3 I 6 8 Well Strip Plates 3 I 7 12 Well Strip Plates 3 I 8 16 Well Strip

More information

Hormones & Chemical Signaling

Hormones & Chemical Signaling Hormones & Chemical Signaling Part 2 modulation of signal pathways and hormone classification & function How are these pathways controlled? Receptors are proteins! Subject to Specificity of binding Competition

More information

Chapter 18. An Introduction to the Endocrine System. Hormone Chemistry

Chapter 18. An Introduction to the Endocrine System. Hormone Chemistry Chapter 18 An Introduction to the Endocrine System Hormone Chemistry Endocrine System Components endocrine system - glands, tissues, and cells that secrete hormones Copyright The McGraw-Hill Companies,

More information

A commitment to quality and continuous improvement

A commitment to quality and continuous improvement Guidance for the Validation of Analytical Methodology and Calibration of Equipment used for Testing of Illicit Drugs in Seized Materials and Biological Specimens A commitment to quality and continuous

More information

Thermo Scientific SIEVE Software for Differential Expression Analysis

Thermo Scientific SIEVE Software for Differential Expression Analysis m a s s s p e c t r o m e t r y Thermo Scientific SIEVE Software for Differential Expression Analysis Automated, label-free, semi-quantitative analysis of proteins, peptides, and metabolites based on comparisons

More information

High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK

High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK Background Unilabs YBS are a bioanalytical CRO based in York (Uk) Copenhagen

More information

SMF Awareness Seminar 2014

SMF Awareness Seminar 2014 SMF Awareness Seminar 2014 Clinical Evaluation for In Vitro Diagnostic Medical Devices Dr Jiang Naxin Health Sciences Authority Medical Device Branch 1 In vitro diagnostic product means Definition of IVD

More information

Drug Testing for Criminal Justice Involved Individuals in Michigan

Drug Testing for Criminal Justice Involved Individuals in Michigan Drug Testing for Criminal Justice Involved Individuals in Michigan Oakland County Drug Court Professionals Barbara M. Hankey, Manager Oakland County Community Corrections Session Goals O To present the

More information

Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns

Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns Jonathan P. Danaceau, Kenneth J. Fountain, and Erin E. Chambers Waters

More information

FDA Public Hearing on Clinical Accuracy Requirements for Point of Care Blood Glucose Meters (BGMs)

FDA Public Hearing on Clinical Accuracy Requirements for Point of Care Blood Glucose Meters (BGMs) FDA Public Hearing on Clinical Accuracy Requirements for Point of Care Blood Glucose Meters (BGMs) Barriers to Overcoming Interferences and Limitations Alan T. Cariski, MD, JD, and Mike Flis, AdvaMed BGM

More information