1 Michele Menotta Ph.D. Department of Biomolecular Sciences University of Urbino "Carlo Bo" Via Saffi Urbino (PU) ITALY Tel: Fax: EDUCATION Jan Ph.D. in Biochemistry University of Urbino Carlo Bo Dissertation title: Identification of genes expressed during the presymbiotic phase of T. borchii-t Americana: Cell signalling phenomena. Advisor: Prof. Vilberto Stocchi Mar B. Sc. Cum laude in Biology University of Urbino Carlo Bo Thesis Title: Genetic variability study, by ITS analysis, of different Tuber species Advisor: Prof. Vilberto Stocchi RESEARCH EXPERIENCE Sep.2012-present Postdoctoral research Department of Biomolecular Sciences University of Urbino Research project: Dexamethasone treatment of AT (Ataxiatelangiectasia) patients. Dexamethasone action in Ataxia- Telangiectasia: seeking the molecular mechanism of phenotype reversion. Sep sep.2012 Postdoctoral research Department of Biomolecular Sciences University of Urbino.
2 Michele Menotta 2 Research project: detection of ODNs-/transcription factors interaction by AFM. Granted by NACBO EU FP6. Research project: Dexamethasone action in Ataxia-Telangiectasia: seeking the molecular mechanism of phenotype reversion. May 2006-Aug Postdoctoral research Department of Biomolecular Sciences University of Urbino. Research project: NF-kB quantification by AFM. Granted by FIRB 2003 NanoMed NANOtechnology for biomedicine. Research project: Drugs delivery by SWNT. Granted by NACBO and NanoMed. Research project (from 2009): Dexamethasone action in Ataxia- Telangiectasia: seeking the molecular mechanism of phenotype reversion. Feb Mar 2006 Postdoctoral research Institute of biochemistry, University of Urbino. Research project: Molecular characterization of T. borchii CDC42, MAPK1 and Rho-GDI. Nov-2000-Nov 2003 Doctoral research Institute of biochemistry, University of Urbino. Research project: Study of cell signaling pathways between T. borchii and T. Americana during pre-symbiosis life stage. Oct-1996-Mar 1999 Undergraduate research Institute of biochemistry, University of Urbino. Research project: Genetic variability of Tuber species by ITS analysis. TEACHING EXPERIENCE Adjunct Professor Proteomics Faculty of Science and Technology. Biology course, University of Urbino.
3 Michele Menotta Adjunct Professor Genetic engineering Faculty of Science and Technology. Biotechnology course, University of Urbino Adjunct Professor Biotechnology laboratory Faculty of Science and Technology. Biotechnology course, University of Urbino Adjunct Lecturer Faculty of Science and Technology. 2 level Master course University of Urbino Teaching Assistant Biotechnology laboratory course, Faculty of Science and Technology, University of Urbino Teaching Assistant Human Biology course Faculty of Health and Physical Exercise, University of Urbino. OTHER EXPERIENXES 02/2005 to 03/2005. Visiting researcher, Institute for Biochemistry I, Joseph-Stelzmann-Strasse 52, D50931 Cologne Advanced AFM methodologies training PARK SYSTEMS KANC 4F lui-dong Suwon South Korea Advanced AFM modules training PARK SYSTEMS, Gambetti Kenologia, Milan Visiting researcher, Nanomed Lab University of Genoa European Park AFM User Meeting Lille (F). TECHNICAL EXPERTISE DNA-RNA extraction and manipulation, gel electrophoresis, blotting, radio isotope handling and labelling.
4 Michele Menotta 4 Phage and yeast library screening. Real time quantitative PCR and microarray analysis. Protein engineering: cdna cloning, mutagenesis, expression in bacteria, yeast, D. discoideum, insects and mammalian cells. Protein purification: column chromatography, FPLC, affinity tag. Protein characterization: SDS PAGE, western blotting, chemical cross-linking, in vitro activity. Protein interaction: Yeast two hybrid screening, pull-down experiments. Mass spectrometry: GC-Ion Trap and ESI-Q-TOF. In gel protein digestion and MS/MS protein identification. De novo MS/MS sequencing. Microscopy: Immunohistochemical / immunofluorescence staining and analysis of cells and tissues. Atomic Force Microscopy: NCM, CM, Tapping and FMM surface imaging, in air and in liquid. Force volume spectroscopy with or without chemically modified tips. Chemical and biological functionalization of AFM tips and surfaces. Data statistical analysis and statistical software skill (i.e. SPSS, PRISM, ORIGIN, R). Computer programming by JAVA, C++, VB PATENTS Paola Fanzio, Giuseppe Firpo, Mauro Magnani, Chiara Manneschi, Michele Menotta, Valentina Mussi, Luca Repetto, Paola Scaruffi, Sara Stigliani, Gian Paolo Tonini, Ugo Valbusa. Device and single-molecule analysis method by means of detection of the collisions of a target molecule on functionalized nanopores. WO A PUBBLICATIONS
5 Michele Menotta 5 1. Paola Fanzio *, Valentina Mussi, Michele Menotta, Giuseppe Firpo, Luca Repetto, Patrizia Guida, Mauro Magnani, and Ugo Valbusa. Protein detection with a dslnafunctionalized nanopore. Biosensors and Bioelectronics. Accepted. 2. Michele Menotta, Sara Biagiotti and Mauro Magnani. Quantitative analysis of FK506 by AFM. Biosensors and Bioelectronics. In revision. 3. Sara Biagiotti, Michele Menotta, Marzia Bianchi, Luciana Chessa and Mauro Magnani. Differential gene expression following dexamethasone administration in ATM-/- lymphoblastoid cell lines. The Journal of Steroid Biochemistry and Molecular Biology Jul;392(1-2): Mauro De Santi, Antonella Antonelli, Michele Menotta, Carla Sfara, Sonja Serafini, Simone Lucarini, Giorgio Brandi and Mauro Magnani. Single-Walled Carbon Nanotubes Functionalization for the Delivery of the Water Insoluble Anticancer Agent Indole-3- carbinol Cyclic Tetrameric Derivative CTet. J. Nanopharmaceutics Drug Delivery 1, (2013). 5. Michele Menotta, Sara Biagiotti, Luciana Chessa and Mauro Magnani. Dexamethasone Partially Rescues Ataxia Telangiectasia-mutated (ATM) Deficiency in Ataxia Telangiectasia by Promoting a Shortened Protein Variant Retaining Kinase Activity. J Biol Chem Oct 10; 287(49): Michele Menotta, Rita Crinelli, Elisa Carloni, Valentina Mussi, Ugo Valbusa and Mauro Magnani. Binding force measurement of NF-kB - ODNs interaction: an AFM based decoy and drug testing tool. Biosensors and Bioelectronics Oct. 15; 28(1): Epub 18 July E. Omiccioli, P. Maltese, M. Menotta, G. Amagliani, M. Magnani. Magnetic capture hybridization technology: a new tool for selective isolation of cell-free circulating DNA from human plasma. JOURNAL OF BIOTECHNOLOGY : Mussi V, Fanzio P, Stigliani S, Scaruffi P, Firpo G, Repetto L, Menotta M, Magnani M, Tonini GP, and Valbusa U. Electrical characterization of DNA-functionalized solid state nanopores for bio-sensing. J Phys Condens Matter Nov 17; 22(45): Epub 2010 Oct 29.
6 Michele Menotta 6 9. Raffaella Pierleoni ; Michele Menotta, Antonella Antonelli ; Carla Sfara; Giordano Serafini; Sabrina Dominici; Maria Elena Laguardia ; Annalisa Salis ; Gianluca Damonte ; Lucia Banci ; Marco Porcu ; Paolo Monini ; Barbara Ensoli ; Mauro Magnani. Effect of the redox state on hiv-1 TAT protein multimerization and cell internalization and trafficking. Mol Cell Biochem Dec;345(1-2): Epub 2010 Aug Antonelli, S Serafini, M Menotta, C Sfara, F Pierigè, L Giorgi, G Ambrosi, L Rossi and M Magnani. Improved cellular uptake of functionalized single-walled carbon Nanotubes. Nanotechnology Oct 22;21(42): Epub 2010 Sep Menotta M, Crinelli R, Carloni E, Bianchi M, Giacomini E, Valbusa U, Magnani M. Labelfree quantification of activated NF-kappaB in biological samples by atomic force microscopy. Biosens Bioelectron Jul 15;25(11): Epub 2010 Apr Crinelli R, Carloni E, Menotta M, Giacomini E, Bianchi M, Ambrosi G, Giorgi L, Magnani M.Oxidized ultrashort nanotubes as carbon scaffolds for the construction of cellpenetrating NF-kappaB decoy molecules. ACS Nano May 25;4(5): Amicucci A, Zambonelli A, Iotti M, Polidori E, Menotta M, Saltarelli R, Potenza L, Stocchi V. Morphological and molecular modifications induced by different carbohydrate sources in Tuber borchii. J Mol Microbiol Biotechnol. 2010;18(2): Epub 2010 Mar Maltese P, Canestrari E, Palma L, Ruzzo A, Corini F, Menotta M, Andreoni F, Latiano A, Annese V, and Magnani M. (2009). High resolution melting (HRM) analysis for the detection of ER22/23EK, BclI, and N363S polymorphisms of the glucocorticoid receptor gene. The Journal of steroid biochemistry and molecular biology 113(3-5): Menotta M., Amicucci A., Basili G., Polidori E., Stocchi V., and Rivero F. (2008). Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii. BMC. Microbiol. 8: Crinelli R., Bianchi M., Menotta M., Carloni E., Giacomini E., Pennati M., and Magnani M. (2008). Ubiquitin over-expression promotes E6AP autodegradation and reactivation of the p53/mdm2 pathway in HeLa cells. Mol. Cell Biochem. 17. Gioacchini A.M., Menotta M., Guescini M., Saltarelli R., Ceccaroli P., Amicucci A., Barbieri E., Giomaro G., and Stocchi V. (2008). Geographical traceability of Italian white truffle
7 Michele Menotta 7 (Tuber magnatum Pico) by the analysis of volatile organic compounds. Rapid Commun. Mass Spectrom. 22: Polidori E., Ceccaroli P., Saltarelli R., Guescini M., Menotta M., Agostini D., Palma F., and Stocchi V. (2007). Hexose uptake in the plant symbiotic ascomycete Tuber borchii Vittadini: biochemical features and expression pattern of the transporter TBHXT1. Fungal. Genet. Biol. 44: Ceccaroli P., Saltarelli R., Guescini M., Polidori E., Buffalini M., Menotta M., Pierleoni R., Barbieri E., and Stocchi V. (2007). Identification and characterization of the Tuber borchii D- mannitol dehydrogenase which defines a new subfamily within the polyol-specific medium chain dehydrogenases. Fungal. Genet. Biol. 44: Menotta M., Amicucci A., Basili G., Rivero F., Polidori E., Sisti D., and Stocchi V. (2007). Molecular characterisation of the small GTPase CDC42 in the ectomycorrhizal fungus Tuber borchii Vittad. Protoplasma 231: Menotta M., Pierleoni R., Amicucci A., Sisti D., Cerasi A., Millo E., Chiarantini L., and Stocchi V. (2006). Characterization and complementation of a Fus3/Kss1 type MAPK from Tuber borchii, TBMK. Mol. Genet. Genomics 276: Cervasi B., Paiardini M., Serafini S., Fraternale A., Menotta M., Engram J., Lawson B., Staprans S.I., Piedimonte G., Perno C.F. et al. (2006). Administration of fludarabine-loaded autologous red blood cells in simian immunodeficiency virus-infected sooty mangabeys depletes pstat-1-expressing macrophages and delays the rebound of viremia after suspension of antiretroviral therapy. J. Virol. 80: Amicucci A., Guidi C., Menotta M., Pierleoni R., Polidori E., Zeppa S., and Stocchi V. Towards a better understending of the ectomycorrhizal fungus Tuber borchii Vittad. Life cycle. Recent Res. Devel. Microbiology, (9)(2005): ISBN: G. Giomaro, D. Sisti, A. Amicucci, C. Guidi, M. Menotta, E. Polidori, R.. Pierleoni, S. Zeppa, A. M. Gioacchini, M. Guescini, V. Stocchi."Sintesi micorrizica in vitro fra piante forestali e funghi del genere Tuber: un modello per lo studio dei meccanismi morfologici e molecolari coinvolti nel processo di micorrizazione". Informatore Botanico Italiano, 37(x, sup.1 parte 1) 2005.
8 Michele Menotta Gioacchini A.M., Menotta M., Bertini L., Rossi I., Zeppa S., Zambonelli A., Piccoli G., and Stocchi V. (2005). Solid-phase microextraction gas chromatography/mass spectrometry: a new method for species identification of truffles. Rapid Commun. Mass Spectrom. 19: Menotta M., Gioacchini A.M., Amicucci A., Buffalini M., Sisti D., and Stocchi V. (2004). Headspace solid-phase microextraction with gas chromatography and mass spectrometry in the investigation of volatile organic compounds in an ectomycorrhizae synthesis system. Rapid Commun. Mass Spectrom. 18: Menotta M., Amicucci A., Sisti D., Gioacchini A.M., and Stocchi V. (2004). Differential gene expression during pre-symbiotic interaction between Tuber borchii Vittad. and Tilia americana L. Curr. Genet. 46: Gioacchini A.M., Menotta M., Polidori E., Giomaro G., and Stocchi V. (2002). Solid-phase microextraction gas chromatography/ion trap mass spectrometry and multistage mass spectrometry experiments in the characterization of germacrene D. J. Mass Spectrom. 37: Amicucci A., Guidi C., Bertini L., Menotta M., Potenza L., Rossi I., Stocchi V.. Identification of white Tuber species by multiplex PCR. Libro degli Atti. Vth International Congres: Science and cultivation of truffle and other edible hypogeous mushrooms. Aix-en- Provence, France 4-6 Marzo Proceedings INTERNATIONAL MEETING PRESENTATIONS 1. Michele Menotta, Sara Biagiotti, Marzia Bianchi, Luciana Chessa and Mauro Magnani. Dexamethasone action in Ataxia-Telangiectasia: seeking the molecular mechanism of phenotype reversion. ATW th - 31st July 2013, Birmingham UK. 2. Sara Biagiotti, Michele Menotta, Marzia Bianchi, Luigia Rossi, Luciana Chessa and Mauro Magnani. Gene expression profiling of Dexamethasone treatment on Ataxia- Telangiectasia cells. ATW th - 31st July 2013, Birmingham UK.
9 Michele Menotta 9 3. Michele Menotta, Sara Biagiotti, Marzia Bianchi, Luigia Rossi, Laura Streppa and Mauro Magnani. Label-free quantification of Tacrolimus by Atomic Force Microscopy. CNBXI 27th-29th June 2012, Varese. 4. Biagiotti S, Menotta M, Bianchi M, Giacomini E, Crinelli R, Savio C, Rossi L, Chessa L, Magnani M. New applications for old drugs: molecular mechanism involved in glucocorticoid analogue action in the treatment of Ataxia Telangiectasia FEBS th-25th June Torino. 5. Michele Menotta, Rita Crinelli, Elisa Carloni, Valentina Mussi, Ugo Valbusa and Mauro Magnani. Binding force measurement of NF-kB - ODNs interaction: an AFM based decoy and drug testing tool. FEBS th-25th June Torino. 6. Elisa Giacomini, Marzia Bianchi, Rita Crinelli, Elisa Carloni, Michele Menotta, Mauro Magnani. Different intron-mediated enhancement mechanisms for the 5'- UTR intron of the human UbC gene. SIB th-17th September Milano 7. E. Carloni, R. Crinelli, M. Bianchi, E. Giacomini, M. Menotta, E. Gabucci and M. Magnani. Role of heat shock transcription factors in the induction of UbC gene expression in MG132-treated cells. SIB th-17th September Milano 8. Michele Menotta, Rita Crinellia, Elisa Carloni, Valentina Mussi, Ugo Valbusa and Mauro Magnani. Binding force measurement of NF-kB - ODNs interaction: an AFM based decoy and drug testing tool. SIB th-17th September Milano 9. Mauro De Santi, Sonja Serafini +, Simone Lucarini ^, Gianluca Ambrosi*, Michele Menotta +, Giorgio Brandi +, Mauro Magnani. Use of nanotubes (SWNTs) to increase bioavailability of Indole-3-Carbinol tetrameric derivative. 2nd NACBO International Nanobiotechnology Conference 7th- 9th July 2009 University of Rome 'Tor Vergata. 10. E. Carloni, *M. Menotta, *E. Giacomini, *M. Bianchi, *S. Dominici, #G. Ambrosi, *M. Magnani and *R. Crinelli Covalent functionalization of SWCNT with ODNs targeting Nuclear Factor - B: toward the construction of cell-penetrating decoy molecules. 2nd NACBO International Nanobiotechnology Conference 7th- 9th July 2009 University of Rome 'Tor Vergata. 11. Michele Menotta, Rita Crinelli, Elisa Carloni, Marzia Bianchi, Elisa Giacomini and Mauro Magnani. Analysis of transcription factor-dna interaction by Atomic Force
10 Michele Menotta 10 Microscopy. 2nd NACBO International Nanobiotechnology Conference 7th- 9th July 2009 University of Rome 'Tor Vergata. 12. Michele Menotta, Rita Crinelli, Marzia Bianchi, Elisa Carloni, Elisa Giacomini and Mauro Magnani. AFM imaging could bring NF-kB to the clinic. National Nanomedicine Congress Auditorium Advanced Biotechnology Center (CBA) Genova November 28-29, Antonella Amicucci1, Michele Menotta1, Giorgio Basili1, Emanuela Polidori2, Paola Ceccaroli1, Vilberto Stocchi1, Francisco Rivero. Opening a window for understanding the cell signaling processes in the filamentous fungus Tuber borchii: RhoGdi, another element in the molecular network th European conference on fungal genetics, Edinburgh 14. Zambonelli A., Amicucci A., Menotta M., Polidori E., Iotti M., Saltarelli R., Potenza L., Stocchi V. - Influence of host plant and different supplies on morphology and gene expression in Tuber borchii. Spoleto Tuber Amicucci A., Zambonelli A., Iotti M., Menotta M., Saltarelli R., Zeppa S., Stocchi V., Morphological and molecular modifications induced by different carbohydrate sources in Tuber borchii Vittad. mycelium, in:, book of Abstracts, SORRENTO, s.n, 2007, pp. 193 (atti di: XIII international Congress on molecular plant-microbe interactions, Sorrento, Italy, luglio R. Crinelli, M. Bianchi, M Menotta, E. Carloni, E. Giacomini, L. Gentilini, M Magnani. Ubiquitin Overexpression leads to stabilization of p53 and increased p21 (cip/waf1) transcription by promoting e6ap autodegradation in HeLa cells. SIB 2006, Riccione, 28-30/09/ M. Bianchi, R. Crinelli, E. Giacomini, L. Palma, M. Menotta, E. Carloni e M. Magnani. Overexpression of wild-type ubiquitin downregulates transcription of endogenous polyubiquitin genes in HeLa cells. SIB 2006, Riccione, 28-30/09/ Zeppa S, Barbieri E, Rossi I, Guescini M, Curci R, Falcieri E, Menotta M e Stocchi V. Analisi dell'espressione genica durante il processo di differenziazione miogenica. II Convegno Internazionale "Il ruolo dell'esercizio fisico nella prevenzione delle malattie e nel miglioramento della qualità della vita", Rimini, maggio 2005.
11 Michele Menotta A. Amicucci, M. Menotta, G. Basili, E. Polidori, D. Sisti, F. Rivero, F. Palma and V. Stocchi"Characterisation of the small GTPase CDC42 in the Ascomycetous fungus Tuber borchii Vittad.: its putative role in the hyphal apical growth".1st International Conference on Environmental, Industrial and Applied Microbiology, BioMicroWorld-2005, Badajoz (Spain), 15-18Marzo Menotta M, Pierleoni R, Polidori E, Palma F, Sisti D, Amicucci A, Stocchi V. MAPK signaling in the ectomycorrhizal system Tuber borchii- Tilia Americana. ECFG7 Copenhagen A.Amicucci, M. Menotta, E. Polidori, F. Palma and V. Stocchi. Molecular cloning of Cdc42 and Rho-GDI genes involved in the actin cytoskeleton development in the ectomycorrhizal fungus T. borchii Vittad. SIB Ferrara M. Menotta, R. Pierleoni, E. Polidori, D. Sisti, A. Amicucci and V. Stocchi. MAPK signaling in the ectomycorrhizal system Tuber borchii- Tilia americana. SIB Ferrara A.M. Gioacchini, M. Menotta, R. pierleoni, D. Agostini, V. Stocchi. Characterization by ESI-Q-TOF tandem Mass spectrometry of phosphorylated proteins involved in ectomycorrhizal symbiosis. Torino XXI SCI giugno A.M. Gioacchini, D. Agostani, G. Piccoli, M. Menotta, L. Vallorani, R. pierleoni, V. Stocchi. Identification of terpenes in Calendula flowers extract by solid-phase microextraction gas chromatography ion trap mass spectrometry. Torino XXI SCI giugno D. Agostini, A.M. Gioacchini, G. Piccoli, M. Buffalini, L. Vallorani, M. Menotta, V. Stocchi. Electrospray Quadruple Time of Flight characterization of Aloe arborescens unfractionated extracts. Torino XXI SCI giugno Menotta M.Early molecular communication between plant and fungus: detection of specifically expressed genes during pre-symbiotic interaction. 6a Riunione Nazionale "A. Castellani" dei Dottorandi di Ricerca in Discipline Biochimiche, Brallo di Pregola (PV), giugno Menotta M., Gioacchini A. M., Buffalini M., Amicucci A., Sisti D. and Stocchi V. Volatile compounds: a role in signalling interaction? 6 ECFG Pisa 6-9 April 2002.
12 Michele Menotta M Menotta, A. Amicucci, A.M. Gioacchini., D. Sisti & V. Stocchi. Early molecular communication between plant and fungus: detection of specifically expressed genes during pre-symbiotic interaction. IMC7 Oslo august Gioacchini A., Menotta M., Zeppa S., Bertini L., Zambonelli A., Stocchi V.. SPME- Gas Chromatography/ion trap mass spectrometry, a new and rapid approach to truffle characterization. 20th Informal Meeting on Mass Spectrometry, May 12-16, M.Menotta, A.M. Gioacchini, A. Amicucci, M. Buffalini, D. Sisti, V. Stocchi"Volatile compounds: a role in mycorrhizal symbiosis signalling interaction?" 20th Informal Meeting on Mass Spectrometry, May 12-16, Gioacchini A.M., Menotta M., Polidori E., Giomaro G., and Stocchi V Solid-phase microextraction gas chromatography/ion trap mass spectrometry and multistage mass spectrometry experiments in the characterization of germacrene D. 20th Informal Meeting on Mass Spectrometry, May 12-16, M. Menotta, A. Amicucci, C. Guidi, E. Barbieri, V. Stocchi. Hypotesis on truffles speciation. TICS Third International Congress on Symbiosis Marburg Germany August Guidi C., Amicucci A., Menotta M., Zambonelli A., Potenza L. and Stocchi V. "Molecular identification of different Tuber species " SIB-BIB Biochimica e Biotecnologie, Villa Olmo (CO) giugno 2000:
CHAPTER ONE: INTRODUCTION... 1 STUDY GOALS AND OBJECTIVES... 1 REASONS FOR DOING THIS STUDY... 1 SCOPE AND FORMAT... 1 METHODOLOGY AND INFORMATION SOURCES... 2 INTENDED AUDIENCE... 2 ANALYST CREDENTIALS...
Understanding the immune response to bacterial infections A Ph.D. (SCIENCE) DISSERTATION SUBMITTED TO JADAVPUR UNIVERSITY SUSHIL KUMAR PATHAK DEPARTMENT OF CHEMISTRY BOSE INSTITUTE 2008 CONTENTS Page SUMMARY
COURSE TITLE COURSE DESCRIPTION CH-00X CHEMISTRY EXIT INTERVIEW All graduating students are required to meet with their department chairperson/program director to finalize requirements for degree completion.
Biotechnology s oratories Biotechnology Name Location Person in Charge Programs Served Courses Served General Biology W12-039 Zahra Yassin General Microbiology M12-132 Aisha Echtibi General (Basic Course)
Doctor of Philosophy in Biochemistry FACULTY OF MEDICAL SCIENCE Naresuan University 73 Doctor of Philosophy in Biochemistry The Biochemistry Department at Naresuan University is a leader in lower northern
Syllabus BIOTECHNOLOGY Spring 2013 Instructor: Atanu Duttaroy, Professor Tel: 202-806-5362 Email: firstname.lastname@example.org Office: Room 336, Just Hall Teaching Assistant: Mr. Subhas Mukherjee Lecture: Room
Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN
Biotechnology and reporter genes Here, a lentivirus is used to carry foreign DNA into chickens. A reporter gene (GFP)indicates that foreign DNA has been successfully transferred. Recombinant DNA continued
Reiner Westermeier, Torn Naven Hans-Rudolf Höpker Proteomics in Practice A Guide to Successful Experimental Design 2008 Wiley-VCH Verlag- Weinheim 978-3-527-31941-1 Preface Foreword XI XIII Abbreviations,
CURRICULUM VITAE RANDOLPH LUCA BRUNO Contact Details Address for Correspondence email@example.com Via dei Contarini 5, Homepage: 20133 Milan (Italy) https://mail.sssup.it/ brunor/ http://www.dse.unibo.it/dse/
Marco Barbariga Translational Neurology Group, Department of Clinical Sciences, Division of Neurology, Wallenberg Neurocentrum SE-221 84 Lund Sweden Place/date of birth: April 08, 1985 Gender: Male Nationality:
M.Sc. in Nano Technology with specialisation in Nano Biotechnology Nanotechnology is all about designing, fabricating and controlling materials, components and machinery with dimensions on the nanoscale,
Dr. Sanjeeva Srivastava IIT Bombay Genomics Transcriptomics Why proteomics? IIT Bombay 2 1 IIT Bombay 3 Genome: The entire sequence of an organism s hereditary information, including both coding and non-coding
Microarrays And Functional Genomics CPSC265 Matt Hudson Microarray Technology Relatively young technology Usually used like a Northern blot can determine the amount of mrna for a particular gene Except
Building innovative drug discovery alliances Evotec Munich Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs Evotec AG, Evotec Munich, June 2013 About Evotec Munich A leader
RNA Viruses A Practical Approac h Alan J. Cann List of protocols page xiii Abbreviations xvii Investigation of RNA virus genome structure 1 A j. Easton, A.C. Marriott and C.R. Pringl e 1 Introduction-the
Introduction to Proteomics Why Proteomics? Same Genome Different Proteome Black Swallowtail - larvae and butterfly Biological Complexity Yeast - a simple proteome 6,113 proteins = 344,855 tryptic peptides
CHEMISTRY Department Office Darwin Hall 300 (707) 664-2119 www.sonoma.edu/chemistry Department Chair Lynn R. Cominsky Administrative Coordinator Cathi Cari-Shudde Faculty Steven Farmer Meng-Chih Su *Dale
BIOTECHNOLOGY Levels: 11-12 Units of Credit: 1.0 CIP Code: 51.1201 Prerequisite: Biology or Chemistry Skill Certificates: #708 COURSE DESCRIPTION is an exploratory course designed to create an awareness
For information call: Tel: (818) 77-81 Fax: (818) 77-08 E-mail: firstname.lastname@example.org Website: http://www.csun.edu/chemistry Or write: Department of Chemistry and Biochemistry California State University,
1. A recombinant DNA molecules is one that is a. produced through the process of crossing over that occurs in meiosis b. constructed from DNA from different sources c. constructed from novel combinations
Protein Purification and Analysis Numbers of genes: Humans ~40,000 genes Yeast ~6000 genes Bacteria ~3000 genes Solubility of proteins important for purification: 60-80% soluble, 20-40% membrane Some proteins
Common Course Topics Biology 1414: Introduction to Biotechnology I Assumptions Students may be enrolled in this course for several reasons; they are enrolled in the Biotechnology Program, they need a science
& APPLIED Total journals: 164 Title Frequency ISSN Country 1. ADVANCES IN APPLIED Irregular 0065 2164 USA 2. ADVANCES IN BIOCHEMICAL Irregular 0724 6145 GERMANY ENGINEERING 3. AFRICAN JOURNAL OF Weekly
Chapter 9 Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA Q&A Interferons are species specific, so that interferons to be used in humans must be produced in human cells. Can you think
BBSRC TECHNOLOGY STRATEGY: TECHNOLOGIES NEEDED BY RESEARCH KNOWLEDGE PROVIDERS 1. The Technology Strategy sets out six areas where technological developments are required to push the frontiers of knowledge
AT&T Global Network Client for Windows Product Support Matrix January 29, 2015 Product Support Matrix Following is the Product Support Matrix for the AT&T Global Network Client. See the AT&T Global Network
Notch 1 -dependent regulation of cell fate in colorectal cancer Referees: PD Dr. Tobias Dick Prof. Dr. Wilfried Roth http://d-nb.info/1057851272 CONTENTS Summary 1 Zusammenfassung 2 1 INTRODUCTION 3 1.1
CUSTOM ANTIBODIES Highly competitive pricing without compromising quality. Rat monoclonal antibodies for the study of gene expression and proteomics in mice and in mouse models of human diseases available.
Common Course Topics Biology 1406: Cell and Molecular Biology 1. Introduction to biology --the scientific study of organisms --properties of life --assumptions, methods and limitations of science --underlying
La Protéomique : Etat de l art et perspectives Odile Schiltz Institut de Pharmacologie et de Biologie Structurale CNRS, Université de Toulouse, Odile.Schiltz@ipbs.fr Protéomique et Spectrométrie de Masse
Biology M.S. and Ph.D. Programs The graduate Biology program at The Catholic University of America is a small, focused, and collegial program that engages in state-of-the-art research in Cell, Microbial,
Modelli innovativi di produzione per lo sviluppo di un processo altamente qualitativo di farmaci biologici Luca Romagnoli, Ph.D. Business Development Manager BIOLOGICAL DRUGS - SOURCES Monoclonal antibodies
Bio 100 DNA Technology 1 Chapter 12 - DNA Technology Among bacteria, there are 3 mechanisms for transferring genes from one cell to another cell: transformation, transduction, and conjugation 1. Transformation
Course Curriculum for Master Degree in Medical Laboratory Sciences/Clinical Biochemistry The Master Degree in Medical Laboratory Sciences /Clinical Biochemistry, is awarded by the Faculty of Graduate Studies
148 Bishop s University 2015/2016 Biochemistry The Biochemistry program at Bishop s is coordinated through an interdisciplinary committee of chemists, biochemists and biologists, providing students with
prosci-inc.com Custom Antibody Services High Performance Antibodies and More Broad Antibody Catalog Extensive Antibody Services CUSTOM ANTIBODY SERVICES Established in 1998, ProSci Incorporated is a leading
Student Portfolios Introduction: The purpose of the student portfolio is three fold. 1] From your perspective it will provide a resource that you can review when it comes time to apply for Grad School,
Biotechnology Chapter 20: Biotechnology: DNA Technology & Genomics The BIG Questions How can we use our knowledge of DNA to: o Diagnose disease or defect? o Cure disease or defect? o Change/improve organisms?
Recombinant DNA technology (genetic engineering) involves combining genes from different sources into new cells that can express the genes. Recombinant DNA technology has had-and will havemany important
NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!
Biotechnology and Recombinant DNA (Chapter 9) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Primary Source for figures and content: Eastern Campus Tortora, G.J. Microbiology
Name Period The AP Biology exam has reached into this chapter for essay questions on a regular basis over the past 15 years. Student responses show that biotechnology is a difficult topic. This chapter
CURRICULUM VITAE Rosella Visintin Positions: Since 2006 Since July 2005 Assistant professor of SEMM (European School of Molecular Medicine). IFOM-IEO Campus Milan - Italy Junior group leader at the European
The Leading Gene Through Screen CRO Molecular Biology Services Molecular Biology drives modern drug discovery. Drug screening, target validation and structural biology processes frequently require recombinant
CRIME LAB FORENSICS Description Crime Lab Forensics, which is a laboratory-based course, will promote and cultivate the development of student s scientific inquiry and scientific method skills, which are
Arabidopsis A Practical Approach Edited by ZOE A. WILSON Plant Science Division, School of Biological Sciences, University of Nottingham OXPORD UNIVERSITY PRESS List of Contributors Abbreviations xv xvu
Genetics 10201232 Faculty of Agriculture and Veterinary Medicine Instructor: Dr. Jihad Abdallah Topic 15:Recombinant DNA Technology 1 Recombinant DNA Technology Recombinant DNA Technology is the use of
DESCRIPTION Biotechnology is designed to create an awareness of career possibilities in the field of biotechnology. Students are introduced to diagnostic and therapeutic laboratory procedures that support
Doctor of Philosophy Program in Microbiology FACULTY OF MEDICAL SCIENCE Naresuan University 171 Doctor of Philosophy Program in Microbiology The time is critical now for graduate education and research
Coordinating unit: 240 - ETSEIB - Barcelona School of Industrial Engineering Teaching unit: 713 - EQ - Department of Chemical Engineering Academic year: Degree: 2016 MASTER'S DEGREE IN CHEMICAL ENGINEERING
TABLE OF CONTENTS PART 1 THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS Chapter 1 Why Gene Cloning and DNA Analysis are Important 1.1 The early development of genetics 1.2 The advent of gene cloning
Appendix 2 Molecular Biology Core Curriculum Websites and Other Resources Chapter 1 - The Molecular Basis of Cancer 1. Inside Cancer http://www.insidecancer.org/ From the Dolan DNA Learning Center Cold
Page 1 IKDT Laboratory IKDT as Service Lab (CRO) for Molecular Diagnostics IKDT lab offer is complete diagnostic service to all external customers. We could perform as well single procedures or complex
Società Botanica Italiana Gruppi di lavoro Biologia Cellulare e Molecolare Biotecnologie e Differenziamento Università degli Studi di Napoli Federico II BIOGEM Organize the Summer School Challenges, methods
An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism
UNDERGRADUATE COURSE DESCRIPTIONS Dept. of Chemistry and Biochemistry Southern Illinois University at Carbondale 106-3 Chemistry and Society. (University Core Curriculum) [IAI Course: P1 903L] Exploration
A simple allele-specific polymerase chain reaction method to detect the Gly143Glu polymorphism in the human carboxylesterase 1 gene: importance of genotyping for pharmacogenetic treatment. Walter Soria
Kazan (Volga region) Federal University, Kazan, Russia Institute of Fundamental Medicine and Biology Master s program Bioinformatics I. THEORETICAL BASIS The development of effective technologies of theoretical
UNIVERSITY OF MARIBOR FACULTY OF CHEMISTRY AND CHEMICAL ENGINEERING INFORMATION PACKAGE / INTERNATIONAL EXCHANGE STUDENTS' GUIDE 2016/2017 Part II Information on study programmes A) General description
Eudendron: an Innovative Biotech Start-up Mauro Angiolini & Fabio Zuccotto I Venti dell Innovazione, Ville Ponti - Varese, 20 Marzo 2013 Bioindustry Park S. Fumero (Ivrea) - Italy Eudendron: a Quick Description
Biotechnology and Recombinant DNA Recombinant DNA procedures - an overview Biotechnology: The use of microorganisms, cells, or cell components to make a product. Foods, antibiotics, vitamins, enzymes Recombinant
Course Curriculum for Master Degree in Medical Laboratory Sciences/Clinical Microbiology, Immunology and Serology The Master Degree in Medical Laboratory Sciences / Clinical Microbiology, Immunology or
KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal
Department of Food and Nutrition Faculties Professors Lee-Kim, Yang Cha, Ph.D. (M.I.T., 1973) Nutritional biochemistry, Antioxidant vitamins, Fatty acid metabolism, Brain development, and Hyperlipidemia
Coffeyville Community College BIOL-206 COURSE SYLLABUS FOR BIOLOGY I: Cellular and Molecular Biology Pam Oliver Instructor COURSE NUMBER: BIOL-206 COURSE TITLE: Biology I: Cellular and Molecular Biology
Protein Expression A Practical Approach S. J. HIGGIN S B. D. HAMES List of contributors Abbreviations xv Xvi i 1. Protein expression in mammalian cell s Marlies Otter-Nilsson and Tommy Nilsso n 1. Introduction
Aiping Lu Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Proteome and Proteomics PROTEin complement expressed by genome Marc Wilkins Electrophoresis. 1995. 16(7):1090-4. proteomics
assays of DEAE chromatographic fractions of cell-free extracts. The specificity of TF2 for the 5 C box was tested by competition analysis using six other oligonucleotides. Purification of TF2 was achieved
www.sciencesignaling.org/cgi/content/full/7/339/ra80/dc1 Supplementary Materials for Manipulation of receptor oligomerization as a strategy to inhibit signaling by TNF superfamily members Julia T. Warren,
1. Introduction Collection Policy: Chemistry The Library supports the instructional and research needs of faculty and students working within the Department of Chemistry. Instruction in Chemistry has been
Enzyme Response Profiling: Integrating proteomics and genomics with xenobiotic metabolism and cytotoxicity Proteomics high throughput separation and characterization of expressed proteins in tissue and
UNR Chemistry Courses Changes to implement the UCCSN Common Course Numbering Initiative Effective beginning with Summer 2003 term Old CHEM courses (2002-2003 catalog) 100 MOLECULES AND LIFE IN THE MODERN
NAV HISTORY OF DBH FIRST MUTUAL FUND () Date NAV 11-Aug-16 10.68 8.66 0.38% -0.07% 0.45% 3.81% 04-Aug-16 10.64 8.66-0.19% 0.87% -1.05% 3.76% 28-Jul-16 10.66 8.59 0.00% -0.34% 0.34% 3.89% 21-Jul-16 10.66
Biology for the Nanotechnology Classroom Erinn Mee email@example.com 10/9/2015 Size and Scale http://learn.genetics.utah.edu/content/cells/scale/scale.html Visualizing Bacteria Escherichia coli LM 1000x
Abstract Abbreviations List of figures CONTENT I-VI VII-VIII IX-XII List of tables XIII Chapter 1 Review of Literature 1. Vaccination against intracellular pathogens 1-34 1.1 Role of different immune responses
i 1 INTRODUCTION 1.1 Human Hepatitis B virus (HBV) 1 1.1.1 Pathogenesis of Hepatitis B 1 1.1.2 Genome organization of HBV 3 1.1.3 Structure of HBV virion 5 1.1.4 HBV life cycle 5 1.1.5 Experimental models
HuCAL Custom Monoclonal Antibodies Highly Specific Monoclonal Antibodies in just 8 Weeks PROVEN, HIGHLY SPECIFIC, HIGH AFFINITY ANTIBODIES IN 8 WEEKS WITHOUT HuCAL PLATINUM IMMUNIZATION (Human Combinatorial
Curriculum Vitae Pramod Kumar Giri, Ph.D Research Associate, Department of Microbiology & Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611 USA. Phone: Lab : 001-312-503-1093
Expression and Purification of Recombinant Protein in bacteria and Yeast Presented By: Puspa pandey, Mohit sachdeva & Ming yu DNA Vectors Molecular carriers which carry fragments of DNA into host cell.
CURRICULUM VITAE AYŞEGÜL ATASOY PERSONAL INFORMATION Date of Birth : 13.03.1988 Date of Place Present Address : Konya : Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics
CHEMISTRY (CHEM) Updated April 15, 2016 Note: The department/program code CHEM replaces the former code 08. Students cannot hold credit in CHEM-xxxx and the former 08.xxxx having the same course number
Chemistry MAJOR, MINOR PROFESSORS: Sandy (chair), Bansi L. ASSOCIATE PROFESSOR: Daniel R. CURATOR: Patricia Tucker By nurturing the student s intellect and by fostering the student s growth of literacy
Program Overview Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Session 2. Principles of Mass Spectrometry Session 3. Mass spectrometry based proteomics
pglo Bacterial Transformation Practical What is transformation? Genetic transformation literally means change caused by genes. It occurs when a cell takes up (takes inside) and expresses a new piece of