EQuan MAX Getting Connected Field Service Guide

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1 EQuan MAX Getting Connected Field Service Guide This guide provides procedures to connect the various components for the Thermo Scientific EQuan MAX automated high-throughput LC-MS system. Contents Hardware Requirements Software Requirements Instrument Connections Access to CTC Methods and Macros Trademarks Hardware Requirements Software Requirements Instrument Connections Accela Open Autosampler and Valve Connections The EQuan MAX system supports configurations for the following hardware: TSQ Series mass spectrometer Exactive mass spectrometer Accela Open Autosampler (non-dlw option) Accela 1250 pump Accela 600 pump These are the minimum software requirements for operating the EQuan MAX system. TSQ Series 2.3 SP1 Exactive 1.1 Xcalibur 2.1 LC Devices 2.4 LCQuan 2.6 (Optional) TraceFinder After installing the software, connect the devices as instructed in the following sections. Accela Open Autosampler and Valve Connections EQuan MAX Plumbing Connections Assemble the Accela Open Autosampler as shown in Figure 1. For information on how to assemble the Accela Open Autosampler, refer to the Accela Open Autosampler Hardware Manual. Revision A Thermo Fisher Scientific Inc. All rights reserved.

2 Figure 1. Accela Open Autosampler Tip For the TSQ mass spectrometer, the setup in Figure 1 is the most effective because the valves are positioned closest to the source. The most effective setup for the Exactive MS places the valves and wash station to the left side and moves the cooled stack to the right. You can adjust the setup according to the customer s requirements. Figure 2 shows the EQuan MAX and conventional HPLC valves. The top-left valve is a low pressure valve (C2V-3006D-CTC) that is used for large volume (EQuan MAX) injections. The valve beneath it is the EQuan MAX 6-port switching valve (C72VX-1969D-CTC). The bottom valve slot on the left is intentionally left empty. Use the single valve on the right side for conventional HPLC (non-equan MAX) injections (C72VX-1969D-CTC). Figure 2. EQuan MAX and conventional HPLC valves EQuan MAX switching valve (C72VX-1969D-CTC) EQuan MAX injection valve (C2V-3006D-CTC) Conventional HPLC (non-equan MAX) injection valve (C72VX-1969D-CTC) 2

3 To make connections on the Accela Open Autosampler back panel and in the valve drive modules 1. Connect the TERMINAL port on the Accela Open Autosampler back panel (see Figure 3) to the handheld controller. The handheld controller is not shown in Figure Connect the SER1 port on the back panel (see Figure 3) to the instrument PC. The PC is not shown in Figure Connect the MODBUS port on the back panel (see Figure 3) to Port A of the non-equan MAX injection valve (see Figure 4). 4. Connect the WASH STATION port on the back panel (see Figure 3) to the wash station. The wash station is not shown in Figure Connect the INTERFACE 1 port on the back panel (see Figure 3) to the Accela 1250 Pump using the dual-cable connector. The Accela 1250 Pump is not shown in Figure Connect the AUX 1 port on the back panel (see Figure 3) to the AUX port in the EQuan MAX injection valve drive module (see Figure 4). 7. Connect the POWER port on the back panel (see Figure 3) to the power module box (not shown in Figure 3), which is connected to the power outlet. The POWER port in Figure 3 is not connected. Figure 3. Accela Open Autosampler back panel connections SER1 WASH STATION TERMINAL MODBUS INTERFACE 1 AUX 1 POWER Accela Open Autosampler back panel 8. Connect the MODBUS port B in the conventional HPLC (non-equan MAX) injection valve drive module to the MODBUS port A in the EQuan MAX switching valve drive module (see Figure 4). 9. Connect Port B in the EQuan MAX switching valve drive module (see Figure 4) to the power module box (not shown in Figure 4), which is connected to the power outlet. Port B of the EQuan MAX switching valive in Figure 4 is currently unconnected. 3

4 Figure 4. Valve drive modules and their connections AUX valve drive module for the EQuan MAX injection valve Serial valve drive module for the EQuan MAX switching valve Port A Serial valve drive module Port B Port A Port B for the conventional HPLC injection valve EQuan MAX Plumbing Connections To connect the EQuan MAX plumbing to the valves 1. Connect Port 5 on the EQuan MAX injection valve to Port 1 (top port) on the EQuan MAX switching valve using the 0.01 in. ID PEEK blue tubing. See Figure 5. Figure 5. Connection of the EQuan MAX injection valve to the EQuan MAX switching valve Port 5 EQuan MAX injection valve Port 1 EQuan MAX switching valve 4

5 2. Connect all three waste lines to the three valves (Port 2 on the EQuan MAX injection valve, Port 6 on the EQuan MAX switching valve, and Port 2 on the conventional HPLC (non-equan MAX) injection valve). See Figure 6. Do not overtighten the stainless steel fittings because doing so can restrict the flow from the valve and cause problems. Fingertight is sufficient. Do not substitute PEEK tubing for the TEFLON tubing, as the ID in the PEEK tubing is too restrictive for waste flows. Figure 6. Connection between the waste lines and the valves Port 2 Port 6 Port 2 EQuan MAX injection valve Stainless steel fittings EQuan MAX switching valve Conventional HPLC (non-equan MAX) injection valve 5

6 3. Connect Port 4 on the EQuan MAX switching valve to Port 5 on the conventional HPLC injection valve using the metal tubing. This line must be metal because it experiences high pressure (U-HPLC). See Figure 7. Figure 7. Connection from the EQuan MAX switching valve to the conventional HPLC injection valve Port 4 (EQuan MAX switching valve) Port 5 (Conventional HPLC injection valve) 4. Connect the conventional HPLC sample loop to the conventional HPLC valve between Port 3 and Port 6. See Figure 8. Figure 8. Connection for the conventional HPLC sample loop Port 3 Port 6 Conventional HPLC sample loop 6

7 5. Connect the loading column to the EQuan MAX switching valve between Port 2 and Port 5 using the metal tubing. See Figure 9. Figure 9. Connection for the loading column Loading column Port 2 Port 5 6. Connect the EQuan MAX sample loop to the EQuan MAX injection valve between Port 3 and Port 6. There are three types of loops to choose from: 1 ml (the default), 5 ml, and 20 ml. Install the default size unless the customer asks for a different size. See Figure 10. Figure 10. Connection for the EQuan MAX sample loop Port 3 Port 6 EQuan MAX sample loop 7

8 7. Using metal tubing, connect Port 4 on the conventional HPLC injection valve to the HPLC eluting pump (bottom pump). See Figure Using blue tubing, connect Port 4 on the EQuan MAX injection valve to the HPLC loading pump (top pump). See Figure 11. See Figure 12 for the two pumps. Figure 11. Connections to the pumps Port 4 To HPLC eluting pump Port 4 To HPLC loading pump 8

9 Figure 12 shows a full view of the valve connections to the two pumps. Figure 12. Valve connections to the pumps From Port 4 on the EQuan MAX injection valve HPLC loading pump From Port 4 on the HPLC injection valve HPLC eluting pump 9

10 9. Using metal tubing, connect Port 3 on the EQuan MAX switching valve to the analytical column (see Figure 13). The analytical column is positioned near the Ion Max housing of the mass spectrometer (see Figure 14). Figure 13. Connection for the analytical column Port 3 To analytical column Figure 14 shows the finished setup of the EQuan MAX system. Figure 14. Finished EQuan MAX system setup Analytical column Ion Max housing Checking the Accela Pump Connections To verify the Accela 1250 and 600 pumps are properly connected 1. From the Xcalibur Home Page, click (Instrument Setup). 2. Click (Accela 1250 Pump) in the viewbar of the Thermo Xcalibur Instrument Setup window. 3. Click the Pump General tab. 4. Confirm the Accela 1250 and 600 pumps are available under Pump 1 and Pump 2 (see Figure 15). 5. Click the Gradient Program tab. 10

11 6. Confirm the gradient programs for Accela 1250 and 600 pumps are available under Pump 1 and Pump 2 (see Figure 16). Figure 15. Confirmation of proper connection for Accela 1250 and 600 pumps (screen 1) Figure 16. Confirmation of proper connection for Accela 1250 and 600 pumps (screen 2) Access to CTC Methods and Macros You can access CTC methods and macros for the EQuan MAX system from the PSE website in the link below: 11

12 Trademarks EQuan, TSQ, Exactive, and LCQuan are trademarks; and Accela and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. PEEK is a trademark of Victrex plc. Teflon is a registered trademark of E.I. du Pont de Nemours and Co. 12

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