Methods in Enzymology. Volume XL VII. Enzyme Structure. Part E. C. H. W. Hirs. Serge N. Timasheff

Transcription

1 Methods in Enzymology Volume XL VII Enzyme Structure Part E C. H. W. Hirs Serge N. Timasheff

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3 CONTRIBUTORS TO VOLUME XLVII PREFACE VOLUMES IN SERIES ix xii i XV Section I. Amino Acid Analysis 1. Subnanomole-Range Analysis P. E. HARE 3 2. Improved Ion-Exchange Resins JAMES R. BENSON Internal Standards for Amino Acid Analysis JAMES F. RIORDAN AN D ROGER W. GIESE Complete Hydrolysis of Polypeptides with In- GARFIELD P. ROYER, solubilized Enzymes JAMES F. RIORDAN, AND FREDERICK A. LIBERATORE High-Performance Liquid Chromatography of CARL L. ZIMMERMAN AND Amino Acid Derivatives JOHN J. PISANO The Use of Logarithmic Plots of Electro - phoretic Mobilities of Peptides R. E. GFFORD 5 1 Section II. End Group Method s 7. Carboxypeptidase C HARALD TSCHESCHE Carboxypeptidase Y in Sequence Determina - tion of Peptides RIKIMARU HAYASHI 84 Section III. Chain Separatio n 9. Gel Filtration C. H. W. Hills 9 7 Section IV. Cleavage of Disulfide Bonds 10. Reductive Cleavage of Cystine Disulfides with URS TH. RÜEGG AN D Tributylphosphine JOSEF RUDINGER Alkylation of Cysteine Thiols with 1,3-Propane URS TH. RÜEGG AN D Sultone JOSEF RUDINGER Reductive Cleavage of S-Sulfo Groups wit h Tributylphosphine URS TH. RÜEGG 123 Section V. Selective Cleavage by Chemical Method s 13. Cleavage at Cysteine after Cyanylation GEORGE R. STARK Cleavage at Asn-Gly Bonds with PAUL BORNSTEIN AN D Hydroxylamine GARY BALIAN Cleavage at Aspartyl-Prolyl Bonds MICHAEL LANDON Phthalylation of Amino Groups J-F. PECHERE AND R. BERTRAND 149

4 17. Reversible Blocking at Arginine by Cyclohexanedionc EMIL L. SMITH 15 6 Section VI. Selective Cleavage with Enzymes 18. Cleavage at Arginine Residues by Clostripain WILLIAM M. MITCHELL Specific Hydrolysis by Trypsin at Alkaline ph LEWIS J. GREENE AND DIANA C. BARTELT Applications of Thermolysin in Protein Struc - tural Analysis ROBERT L. HEINRIKSON Cleavage at Glutamic Acid with Staphylococcal Protease GABRIEL R. DRAPEAU 18 9 Section VII. Separation of Peptides 22. Two-Dimensional Thin-Layer Methods ROBERT W. GRACY Separation of Peptides on Phosphocellulose CHRISTOPHER C. Q. CHI N and Other Cellulose Ion Exchangers AND FINN WOLD Chromatography on Microbore Columns WERNER MACHLEIDT, JOACHIM OTTO, AN D ELMAR WACHTER Automated Micro Procedures for Peptide ALAN C. HERMAN AN D Separations THOMAS C. VANAMAN Detection of Peptides by Fluorescence Methods C. Y. LAI 236 Section VIII. Sequence Analysi s 27. Microsequence Analysis in Automated Spinning-Cup Sequenators JACK SILVER Peptide Methodology with Braunitzer Reagents RAYMOND L. BLAKLEY New Supports in Solid-Phase Sequencing WERNER MACHLEIDT AN D ELMAR WACHTER Coupling Techniques in Solid-Phase Se - quencing RICHARD A. LAURSEN Alternative Reagents in Sequential Degrada - tion on Solid-Phase Supports ALDO PREVIERO Solid-Phase Sequencing in Spinning-Cup Se - quenators DENNIS A. POWERS High-Sensitivity Sequence Determination of Immobilized Peptides and Proteins JOHN BRIDGEN Improved Manual Sequencing Methods GEORGE E. TARR Solid-Phase COOH-Terminal Sequential De - gradation ANDRE DARBRE Regeneration of Amino Acids from Anili - nothiazolinones C. Y. LAI Identification of Anilinothiazolinones after Rapid Conversion to N'-Phenylthiocar- JoHN K. INMAN AND bamylamino Acid Methylamides ETTORE APPELLA Automated Conversion of Anilinothiazolinone s into PTH Derivatives JOHN BRIDGEN 385

5 39. Analysis of Dipeptides by Gas Chromatography-Mass Spectrometry and Applicatio n to Sequencing with Dipeptidyl Aminopep- HENRY C. KRUTZSCH AND tidases JOHN J. PISANG 39 1 Section IX. Chemical Modification 40. Novel Sulfhydryl Reagents GEORGE L. KENYON AN D THOMAS W. BRUICE Modification of Histidyl Residues in Protein s by Diethylpyrocarbonate EDITH WILSON MILES Modification of Tryptophan to Oxindolylalanine by Dimethylsulfoxide-Hydrochloric WALTER E. SAVIGE AN D Acid ANGELO FONTANA Interconversion of Methionine and Methionine WALTER E. SAVIGE AN D Sulfoxide ANGELO FONTANA Cleavage of the Tryptophanyl Peptide Bond WALTER E. SAVIGE AN D by Dimethylsulfoxide-Hydrobromic Acid ANGELO FONTANA Reductive Alkylation of Amino Groups GARY E. MEANS Active-Site-Directed Reagents of Glycolytic FRED C. HARTMAN AN D Enzymes I. LUCILE NORTON 479 Section X. Methods of Peptide Synthesis 47. The Synthesis of Peptides by Homogeneous PANAYOTIS G. KATSOYANNIS Solution Procedures AND GERALD P. SCHWARTZ Solid Phase Peptide Synthesis MARILYNN S. DOSCHER 578 AUTHOR INDEX 61 9 SUBJECT INDEX 641