Supplementary Materials and Methods (Metabolomics analysis)
|
|
- John Harvey Patterson
- 2 years ago
- Views:
Transcription
1 Supplementary Materials and Methods (Metabolomics analysis) Metabolite extraction and mass spectrometry analysis. 12 Vldlr-/- knock out and 12 wild type (WT) retinas were separately frozen at -80 ºC in 50µL PBS until used, at which point metabolites were extracted and proteins were precipitated by adding 600µL of cold acetone to the cell pellet. Samples were then vortexed-mixed for 30 sec and submerged for 1 min in liquid nitrogen followed by thawing (2-3 minutes) and sonication for 10 min at 50 ºC. This process was repeated three times followed by freezing at -20 ºC for 1h. The pellet was removed by centrifugation at 16,000 x g for 10 min and the supernatant was transferred to a clean tube. 400 µl of cold methanol/water/formic acid (86.5/12.5/1.0) was added to the pellet followed by vortexmixing for 30 sec, sonication for 15 min at 50 ºC and freezing for 60 min at -20 ºC. The pellet was again removed by centrifugation at 16,000 x g for 10 min, and supernatant was pooled with the first extraction in the clean tube. Samples were dried in a SpeedVac to dryness. 100 µl of 95% ACN/water was added to the dried samples followed by vortex-mixing and sonication in a bath sonicator for 5 min. Samples were centrifuged at 16,000 x g and the supernatant was removed to a clean LC-MS vial. The samples were maintained at 4 ºC in the autosampler and analyzed by LC-MS. Liquid chromatography-ms (LC-MS). 4 µl of processed retina sample were injected for each run. Reverse phase chromatography was performed using a 150 by 0.5mm (diameter) Zorbax C18 column with 5µm particles, at a flow rate of 20 µl/min. The LC system was an Agilent 1100 with a capillary pump. Buffer A was water with (+ ion mode) or without (- ion mode) 0.1% formic acid, and buffer B was acetonitrile with (+ ion mode) or without (- ion mode) 0.1% formic acid. For the endcapped C18 column, the column was equilibrated in 90% buffer A, and the samples were run with a gradient of 10%-98% over 60min. HILIC chromatography was performed using a 150 by 1.0mm (diameter) SeQuant ZIC -HILIC column with 3.5µm particles, at a flow rate of 58
2 50 µl/min. The LC system was an Agilent 1100 with a capillary pump. Buffer A was water in 5mM ammonium acetate, and buffer B was 90% acetonitrile/10% water in 5mM ammonium acetate. The column was equilibrated in 90% B, and the samples were rung using a gradient of 90%-40% over 15min. Mass Spectrometry. Data was collected in continuum mode from m/z 100 to 1000 using an Agilent ESI-TOF. The capillary voltage was 3500 V, with a nebulizer gas flow of 15 L/min. The instrument was calibrated immediately before use. The data files were converted from the instrument format (.wiff) to the common data format, using the PESciEX data translator. The XCMS program (Ref 1) was used to align and analyze the LC-MS data. To confirm the identification of significant ions, we used a quadrupole-tof (Agilent 6520 Accurate-Mass Q- TOF). Masses for CID were targeted from a mass list and fragmented during the chromatography run. Metabolite Database searching. Metabolites were identified by exact mass (m/z) using METLIN (http://metlin.scripps.edu/), Human Metabolome Project (http://redpoll.pharmacy.ualberta.ca/hmdb/hmdb/), Lipid Maps (http://www.lipidmaps.org/), Biological Magnetic Resonance Data Bank (http://www.bmrb.wisc.edu/) and KEGG ligand (http://www.genome.jp/kegg/ligand.html) databases. 59
3 Supplemental figure S1: Metabolomics analysis discovered multiple carnitine derivatives upregulated in Vldlr-/- retinas compared with WT retinas. (A) Metabolites from each isolated retina were extracted using acetone/methanol and applied to a capillary reversed phase (RP) or HILIC HPLC column interfaced to an ESI-TOF mass spectrometer in positive (+) and negative (-) ion mode to increase metabolite coverage (ref 1). Processing of raw data using the XCMS software resulted in time-aligned ion features, which were defined as a unique m/z at a unique retention time (ref 2) features were found in the wild type (WT) and VLDLR-/- retina. Statistically significant differences were ranked using t-test statistical analyses in order to obtain quantitative information between WT and Vldlr-/- samples. (B) Identification of metabolites: accurate mass information by TOF (error < 5ppm) was used for metabolite database searching. Test compounds were selected for each metabolite identified, and their HPLC retention times and collision-induced dissociation (CID) fragmentation patterns (Q-TOF MS) were compared with identified metabolite for conclusive confirmation of identification. Blue asterisks correspond to parent ions, exemplifying the identification of endogenous L-carnitine. Selected references: 1. Smith, C. A., Want, E. J., O'Maille, G., Abagyan, R. & Siuzdak, G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Anal Chem 78, (2006). 2. Nordstrom, A., Want, E., Northen, T., Lehtio, J. & Siuzdak, G. Multiple ionization mass spectrometry strategy used to reveal the complexity of metabolomics. Anal Chem 80, (2008). 60
4 Supplemental Figure S1: 61
5 Supplemental table 1: Large-scale microarray gene expression analysis was performed to test the expression of oxidative stress-related genes, particularly those involved in cellular defense mechanisms against oxidative stress. Overall, no differences in gene expression of these factors were found when comparing 3 week old Vldlr-/- mouse retinas to age-matched C57BL6/J wild-type retinas indicating that the observed increase in oxidative stress and subsequent neuronal degeneration is not directly related to the Vldlr-/- genetic defect, but rather is likely to be a consequence of the excessive intra- and subretinal NV that forms in the retinas of Vldlr-/- mice. Table 1 gives the raw data values of all the tested genes (obtained from the microarray analysis following the standard affymetrix protocol) that were found to be expressed in the retinas. Genes whose values differed >2-fold between Vldlr-/- and WT retinas are shown in red. Only one outlier, 9-cis-retinol dehydrogenase, demonstrated significantly different expression levels (>95%), although we have yet to determine a functional correlation between the expression levels of this gene and the Vldlr-/- retinal phenotype. 62
6 50
7 51
Simultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF
Simultaneous qualitative and quantitative analysis using the Agilent 654 Accurate-Mass Q-TOF Technical Overview Authors Pat Perkins Anabel Fandino Lester Taylor Agilent Technologies, Inc. Santa Clara,
CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in
Method Development of LC-MS/MS Analysis of Aminoglycoside Drugs: Challenges and Solutions
Method Development of LC-MS/MS Analysis of Aminoglycoside Drugs: Challenges and Solutions authors Angela (Qi) Shen, Ling Morgan, Marcele L. Barroso, and Xin Zhang; Tandem Labs Tuyen Nguyen; Sepracor Inc.
Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology
Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging
A Streamlined Workflow for Untargeted Metabolomics
A Streamlined Workflow for Untargeted Metabolomics Employing XCMS plus, a Simultaneous Data Processing and Metabolite Identification Software Package for Rapid Untargeted Metabolite Screening Baljit K.
UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs
Application Note: 439 UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela UHPLC System MSQ Plus MS Detector Drugs
The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis
The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis Stephen Lock AB SCIEX Warrington (UK) Overview A rapid, robust, sensitive and specific LC-MS/MS method has been developed for
Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples
Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed
Electrospray Ion Trap Mass Spectrometry. Introduction
Electrospray Ion Source Electrospray Ion Trap Mass Spectrometry Introduction The key to using MS for solutions is the ability to transfer your analytes into the vacuum of the mass spectrometer as ionic
Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS
Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,
An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol
An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol Sarah Fair, Dayana Argoti, Joseph Herman, Thermo Fisher Scientific,
Fast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan
Fast, Reproducible LC-MS/MS Analysis of and Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 685 Key Words Accucore C18, dextromethorphan, dextrorphan, SOLA CX Abstract
Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz
Toxichem Krimtech 211;78(Special Issue):324 Online extraction LC-MS n method for the detection of drugs in urine, serum and heparinized plasma Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische
Guide to Reverse Phase SpinColumns Chromatography for Sample Prep
Guide to Reverse Phase SpinColumns Chromatography for Sample Prep www.harvardapparatus.com Contents Introduction...2-3 Modes of Separation...4-6 Spin Column Efficiency...7-8 Fast Protein Analysis...9 Specifications...10
Overview. Purpose. Methods. Results
A ovel Approach to Quantify Unbound Cisplatin, Carboplatin, and xaliplatin in Human Plasma Ultrafiltrate by Measuring Platinum-DDTC Complex Using LC/M/M Min Meng, Ryan Kuntz, Al Fontanet, and Patrick K.
Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay
PO-CON1484E Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching ASMS 014 TP85 David R. Baker 1, John Warrander 1, Neil Loftus 1, Simon Hird
Analysis of Intact Antibodies using HPLC-Chip technology combined with True High Definition QTOF Mass Spectrometry
Analysis of Intact Antibodies using HPLC-Chip technology combined with True High Definition QTOF Mass Spectrometry Ravindra Gudihal Application Scientist Agilent Technologies, India Structure and modifications
LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research
LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research J. Jones, J. Denbigh, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20581 Key Words SPE, SOLA,
Advances in Steroid Panel Analysis with High Sensitivity LC/MS/MS. Kenneth C. Lewis 1, Lisa St. John-Williams 1, Changtong Hao 2, Sha Joshua Ye 2
Advances in Steroid Panel Analysis with High Sensitivity LC/MS/MS Kenneth C. Lewis 1, Lisa St. John-Williams 1, Changtong Hao 2, Sha Joshua Ye 2 1 OpAns LLC, Durham, NC, USA; 2 Ionics Mass Spectrometry
Method development for analysis of formaldehyde in foodsimulant. melamine-ware by GC-MS and LC-MS/MS. Internal Technical Report
of melamine-ware by GC-MS and LC-MS/MS Page 1 of 15 Method development for analysis of formaldehyde in foodsimulant extracts of melamine-ware by GC-MS and LC-MS/MS December 2012 Contact Point: Chris Hopley
Introduction to mass spectrometry (MS) based proteomics and metabolomics
Introduction to mass spectrometry (MS) based proteomics and metabolomics Tianwei Yu Department of Biostatistics and Bioinformatics Rollins School of Public Health Emory University September 10, 2015 Background
Q-TOF User s Booklet. Enter your username and password to login. After you login, the data acquisition program will automatically start.
Q-TOF User s Booklet Enter your username and password to login. After you login, the data acquisition program will automatically start. ~ 1 ~ This data acquisition window will come up after the program
Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis
Application Note AN844 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Page 1 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis Introduction Catecholamines
Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS
Summary: Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS UCT Part Numbers: CUNAX226 - Clean-Up C8+NAX, 2mg/6mL BETA-GLUC- ml Beta-Glucuronidase Enzyme, liquid form SLAQ1ID21-3UM -
High Resolution LC-MS Data Output and Analysis
High Resolution LC-MS Data Output and Analysis Your Sample LC/MS Result Sample Sample Efficient Separation Preparation Introduction Gradient (column) Today Data (computer) LC- MS Ions Detection Ions Separation
Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and ACQUITY QDa Detector
Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and ACQUITY QDa Detector Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess
What Do We Learn about Hepatotoxicity Using Coumarin-Treated Rat Model?
What Do We Learn about Hepatotoxicity Using Coumarin-Treated Rat Model? authors M. David Ho 1, Bob Xiong 1, S. Stellar 2, J. Proctor 2, J. Silva 2, H.K. Lim 2, Patrick Bennett 1, and Lily Li 1 Tandem Labs,
Separation and Determination of Liposomal and Non-Liposomal (Free) Doxorubicin from Human Plasma by SPE and LC-MS/MS
Separation and Determination of Liposomal and Non-Liposomal (Free) from Human Plasma by SPE and LC-MS/MS Krishna Rao Dara, Dr. Tushar N. Mehta, Tim Liddicoat, Asia Pacific Center of Excellence, Thermo
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Fig. S1: Effect of ISO- and TAC-treatments on the biosynthesis of FAS-II elongation products in M. tb H37Ra. LC/MS chromatograms showing a decrease in products with elemental compositions
Henry Shion, Robert Birdsall, Steve Cubbedge, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS
Development of Integrated Informatics Workflows for the Automated Assessment of Comparability for Antibody Drug Conjugates (ADCs) Using LC-UV and LC-UV/MS Henry Shion, Robert Birdsall, Steve Cubbedge,
Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction
Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)
Analysis of Pesticides in Vegetables Using the Agilent 1260 Infinity Analytical SFC System with Triple Quadrupole MS Detection
Analysis of Pesticides in Vegetables Using the Agilent 1260 Infinity Analytical SFC System with Triple Quadrupole MS Detection Application Note Food Testing & Agriculture Authors Edgar Naegele and Thomas
Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform
Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform Charles Yang, Leo Wang, Dipankar Ghosh, Jonathan Beck,
AppNote 7/2009. Analysis of Drugs of Abuse using Automated Disposable Pipette Extraction and LC/MS/MS KEYWORDS ABSTRACT
AppNote 7/2009 Analysis of Drugs of Abuse using Automated Disposable Pipette Extraction and LC/MS/MS Fred D. Foster, John R. Stuff, Edward A. Pfannkoch Gerstel, Inc., 701 Digital Dr. Suite J, Linthicum,
Application Note # ET-29 Screening for novel natural products from myxobacteria using LC-MS and LC-NMR
Application Note # ET-29 Screening for novel natural products from myxobacteria using LC-MS and LC-NMR Introduction Because known pathogens become increasingly resistant to antibiotics, and at the same
SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma
SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from uman Plasma Krishna Rao Dara, Dr. Tushar N. Mehta, Asia Pacific Center of Excellence, Thermo Fisher Scientific, Ahmedabad, India Application
Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection
Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Jeff Layne, Michael McCoy, and Sky Countryman Phenomenex, Inc., 411 Madrid Ave.,
Application Note. Determination of Nitrite and Nitrate in Fruit Juices by UV Detection. Summary. Introduction. Experimental Sample Preparation
Application Note Determination of Nitrite and Nitrate in Fruit Juices by UV Detection Category Food Matrix Fruit Juice Method HPLC Keywords Ion pair chromatography, fruit juice, inorganic anions AZURA
Removal of Beta-Glucuronidase Enzyme from Urine Post-Hydrolysis. and Column Lifetime
Removal of Beta-Glucuronidase Enzyme from Urine Post-Hydrolysis to Improve Assay Performance and Column Lifetime Shahana Huq, Seyed Sadjadi, and Sky Countryman Phenomenex, Inc., 411 Madrid Ave., Torrance,
Aiping Lu. Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn
Aiping Lu Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Proteome and Proteomics PROTEin complement expressed by genome Marc Wilkins Electrophoresis. 1995. 16(7):1090-4. proteomics
Structural Analysis of Labeled N-Glycans from Proteins by LC-MS/MS Separated Using a Novel Mixed-Mode Stationary Phase
Structural Analysis of Labeled N-Glycans from Proteins by LC-MS/MS Separated Using a Novel Mixed-Mode Stationary Phase Udayanath Aich 1, Julian Saba 2, Xiaodong Liu 1, Srinivasa Rao 1, and Chris Pohl 1
LC-MS/MS for Chromatographers
LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,
AppNote 1/2012. Rapid Cleanup and Comprehensive Screening of Pain Management Drugs in Urine using Automated Disposable Pipette Extraction and LC-MS/MS
AppNote 1/2012 Rapid Cleanup and Comprehensive Screening of Pain Management Drugs in Urine using Automated Disposable Pipette Extraction and LC-MS/MS Oscar G. Cabrices, Fred D. Foster, John R. Stuff, Edward
METABOLOMICS: LC-MS ANALYSIS DEVELOPMENT
METABLMICS: LC-MS ANALYSIS DEVELPMENT V.V. Tolstikov 1, N. Tanaka 2 and. Fiehn 1 1 - Max Planck Institute of Molecular Plant Physiology, Dept. Lothar Willmitzer, 14424 Potsdam, Germany 2 - Kyoto Institute
Jennifer L. Simeone and Paul D. Rainville Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S INT RO DU C T ION
A Validated Liquid-Liquid Extraction Method with Direct Injection of Hexane for Clopidogrel in Human Plasma Using UltraPerformance Convergence Chromatography (UPC 2 ) and Xevo TQ-S Jennifer L. Simeone
Appendix 5 Overview of requirements in English
Appendix 5 Overview of requirements in English This document is a translation of Appendix 4 (Bilag 4) section 2. This translation is meant as a service for the bidder and in case of any differences between
Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites
Advantages of Polar, Reversed- Phase HPLC Columns for the Analysis of Drug Metabolites Carmen T. Santasania and David S. Bell SUPELCO, 595 North Harrison Road, Bellefonte, PA 16823 T404045 GUS Abstract
RapidFire for High Throughput Screening in early Drug Discovery
RapidFire for High Throughput Screening in early Drug Discovery Fast but do we stay on track? Markus Trunzer ADME Profiling in Basel High analytical burden ~8 000 compounds per year by HT-Sol and HT-Perm
IC-MS and LC-MS Determination of Ionic Liquids, Counterions, and Impurities
ICMS and LCMS Determination of Ionic Liquids, Counterions, and Impurities Leo Wang, Detlef Jensen, and William Schnute Dionex Corporation, Sunnyvale, CA, USA; Dionex Corporation, Olten, Switzerland INTRODUCTION
Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract
Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate
A feasibility study of the determination of B-group vitamins in food by HPLC with Mass Spectrometric detection. LGC/R/2011/181 LGC Limited 2011
A feasibility study of the determination of B-group vitamins in food by HPLC with Mass Spectrometric detection LGC/R/2011/181 LGC Limited 2011 GC11/0005A A feasibility study of the determination of B-group
Chemical analysis service, Turner s Green Technology Group
Chemical analysis service, Turner s Green Technology Group Hourly costs, Academic collaborations leading to co-publications: 627 SEK/hr (excl. VAT) Hourly costs, Industry/Academic collaborations, no publications:
Peptide synthesis, radiolabelling and radiochemical analysis
SUPPLEMENTAL DATA MATERIALS AND METHODS Peptide synthesis, radiolabelling and radiochemical analysis Solid phase synthesis of peptides was carried out on using ABI 433A peptide synthesizer, on a preloaded
Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography
Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography INTRODUCTION The analysis of soft drinks for caffeine was able to be performed using UV-Vis. The complex sample
Increasing Quality While Maintaining Efficiency in Drug Chemistry with DART-TOF MS Screening
Increasing Quality While Maintaining Efficiency in Drug Chemistry with DART-TOF MS Screening Application Note Forensics Author Erin Shonsey Director of Research Alabama Department of Forensic Science Abstract
Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection
Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Derek Hillbeck, Thermo Fisher Scientific, Runcorn, UK Application Note 2999 Key
High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK
High sensitivity assays using online SPE-LC-MS/MS -How low can you go? Mohammed Abrar Unilabs York Bioanalytical solutions, York, UK Background Unilabs YBS are a bioanalytical CRO based in York (Uk) Copenhagen
Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column
Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Sachiki Shimizu, FUJIFILM Fine Chemicals Co., Ltd., Kanagawa, Japan Kenneth J. Fountain, Kevin Jenkins, and Yoko Tsuda,
Monoclonal Antibody Fragment Separation and Characterization Using Size Exclusion Chromatography Coupled with Mass Spectrometry
Monoclonal ntibody Fragment Separation and haracterization Using Size Exclusion hromatography oupled with Mass Spectrometry uthors Haiying hen Katherine McLaughlin Sepax Technologies, Inc. 5 Innovation
PosterREPRINT AN LC/MS ORTHOGONAL TOF (TIME OF FLIGHT) MASS SPECTROMETER WITH INCREASED TRANSMISSION, RESOLUTION, AND DYNAMIC RANGE OVERVIEW
OVERVIEW Exact mass LC/MS analysis using an orthogonal acceleration time of flight (oa-tof) mass spectrometer is a well-established technique with a broad range of applications. These include elemental
Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS
Therapeutic Drug Monitoring of Antiretroviral Drugs with PLC-M Ursula Gutteck-Amsler, Katharina M. Rentsch Abstract Prospective and retrospective studies have provided some evidence of the clinical and
Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2
Technical ote Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Alek. Dooley, Carmai Seto, esham Ghobarah, and Elliott B. Jones verview:
SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC
SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC Katja SÄRKKÄ, Kari ARINIEMI, Pirjo LILLSUNDE Laboratory of Substance Abuse, National Public Health Institute Manerheimintie,
AppNote 1/2014. Automated Hydrolysis, DPX Extraction and LC/MS/MS Analysis of Pain Management Drugs from Urine KEYWORDS ABSTRACT
AppNote 1/2014 Automated Hydrolysis, DPX Extraction and LC/MS/MS Analysis of Pain Management Drugs from Urine Fred D. Foster, John R. Stuff, Edward A. Pfannkoch GERSTEL, Inc., 701 Digital Dr. Suite J,
for mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry
Thermo Scientific Pierce Controls and Standards for Mass Spectrometry calibration tools for mass spectrometry Ensure confidence in instrument performance with Thermo Scientific Pierce Calibration Solutions
Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns
Hydrophilic-Interaction Chromatography (HILIC) for LC-MS/MS Analysis of Monoamine Neurotransmitters using XBridge BEH Amide XP Columns Jonathan P. Danaceau, Kenneth J. Fountain, and Erin E. Chambers Waters
Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System
Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Olaf Scheibner, Maciej Bromirski, Thermo Fisher Scientific, Bremen, Germany
Metabolomics Software Tools. Xiuxia Du, Paul Benton, Stephen Barnes
Metabolomics Software Tools Xiuxia Du, Paul Benton, Stephen Barnes Outline 2 Introduction Software Tools for LC-MS metabolomics Software Tools for GC-MS metabolomics Software Tools for Statistical Analysis
In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates
In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics
Integrated Data Mining Strategy for Effective Metabolomic Data Analysis
The First International Symposium on Optimization and Systems Biology (OSB 07) Beijing, China, August 8 10, 2007 Copyright 2007 ORSC & APORC pp. 45 51 Integrated Data Mining Strategy for Effective Metabolomic
Ádám Tölgyesi Joerg Stroka. Report EUR 26496 EN
Report on the development of a method for the determination of Alternaria toxins and citrinin in wheat, tomato juice and sunflower seeds by liquid chromatography tandem mass spectrometry Ádám Tölgyesi
MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis
MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis Overview MarkerView software is a novel program designed for metabolomics applications and biomarker profiling workflows 1. Using
Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System
Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS
Thermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance
Thermo Scientific SOLA SPE cartridges and plates Technical Guide Join the revolution unparalleled performance Join the revolution next-generation SPE Thermo Scientific SOLA products revolutionize Solid
Protein Precipitation Protocols
Protein Precipitation Protocols Notes: All reagents need to high purity/hplc quality. All tubes used should be new or hand cleaned thoroughly with Micro90 detergent. High quality water needs to be used
A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS
A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS AB SCIEX QTRAP 4500 LC/MS/MS System and Gerstel, Inc. MultiPurpose Sampler
Collagen analysis in fish tissue using the total collagen assay. Application note April 2013
Collagen analysis in fish tissue using the total collagen assay Application note April 2013 Summary The QuickZyme Total Collagen assay provides an easy and fast method to determine collagen quantities
Development of Headspace Gas Chromatography-Mass Spectrometry for Determination of Residual Monomer in Polymer Latex
Journal of Metals, Materials and Minerals, Vol.20 No.3 pp.145-149, 2010 Development of Headspace Gas Chromatography-Mass Spectrometry for Determination of Residual Monomer in Polymer Latex Nopparat THAWEEWATTHANANON
Innovative vs Traditional
And in the red corner, introducing the challenger NASDAQ April 4 2011 243.47 $ 1.38 $ 0.58 % 0.07 $ 0.02 $ 39.4 % Technology DVD Digital Streamline Instant Play Internet Access Unlimited Selection No Late
Salman Azimi, 1 Nayan S. Mistry, 2 and Michelle Wood 2. Drug Quality Control Laboratory, Supreme Council of Health, Doha, Qatar 2
UPLC/MS/MS for the Screening, Confirmation, and Quantification of Drugs Illegally Added to Herbal/Dietary Supplements for the Enhancement of Male Sexual Performance Salman Azimi, 1 Nayan S. Mistry, 2 and
# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water
Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system
Discovery of Pesticide Protomers Using Routine Ion Mobility Screening
Michael McCullagh, 1 David Eatough, 1 Vincent Hanot, 2 and Séverine Goscinny 2 1 Waters Corporation, Wilmslow, UK 2 Wetenschappelijk Instituut Volksgezondheid Institut Scientifique de Santé Publique, Brussels,
CORESTA RECOMMENDED METHOD N 72
CORESTA RECOMMENDED METHOD N 72 DETERMINATION OF TOBACCO-SPECIFIC NITROSAMINES IN SMOKELESS TOBACCO PRODUCTS BY LC-MS/MS (February 216). INTRODUCTION The CORESTA Smokeless Tobacco Sub-Group studied various
HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
Application Note Mass Spectrometry HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer Beer represents one of the most widely consumed beverages in the world. Its annual worldwide production, measured
HRMS in Clinical Research: from Targeted Quantification to Metabolomics
A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier
Novel Method for Solid Phase Peptide Synthesis Using Microwave Energy
Novel Method for Solid Phase Peptide Synthesis Using Microwave Energy Jonathan M. Collins, Michael J. Collins, Rebecca C. Steorts CEM Corporation, Matthews, NC 28106-0200, U.S.A. Presented at American
Investigating Biological Variation of Liver Enzymes in Human Hepatocytes
Investigating Biological Variation of Liver Enzymes in Human Hepatocytes MS/MS ALL with SWATH Acquisition on the TripleTOF Systems Xu Wang 1, Hui Zhang 2, Christie Hunter 1 1 AB SCIEX, USA, 2 Pfizer, USA
The basic division explosives for forensic purposes is shown in Fig. 1
Isolation, concentration and determination of post blast residua by liquid chromatography with DAD detector capillary electrophoresis with DAD detector Firearm fumes (gunshot residua, GSR) - various metallic
STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD 175 STANFORD, CA 94305-5080
Training on the ZQ Open access MS Questions? Contact Dr. Allis Chien allis@stanford.edu 650-723-0710 0710 STANFORD UNIVERSITY MASS SPECTROMETRY STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation Craig Aurand 1, David Bell 1, Anders Fridstrom 2, and Klaus Buckendahl 2 1 Supelco, Div.
Protein Hit1, a novel box C/D snornp assembly factor, controls cellular concentration of protein Rsa1p by direct interaction
Supplementary data Protein Hit1, a novel box C/D snornp assembly factor, controls cellular concentration of protein Rsa1p by direct interaction Benjamin Rothé, Jean-Michel Saliou, Marc Quinternet, Régis
Opiates in Urine by SAMHSA GC/MS
application Note Gas Chromatography/ Mass Spectrometry Author Timothy D. Ruppel PerkinElmer, Inc. Shelton, CT 06484 USA Opiates in Urine by SAMHSA GC/MS Introduction The United States Department of Health
An In-Gel Digestion Protocol
An In-Gel Digestion Protocol This protocol describes the digestion of a protein present in an SDS-PAGE gel band with trypsin. The band can be taken from either a 1D or 2D electrophoresis gel. Reagents
Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method
Simultaneous Quantitation of 4 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Xiang He and Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 76 Key Words TSQ Quantum Access