Kaustubha Qanungo Ph.D Biological Sciences Trident Technical College 7000 Rivers Avenue Charleston SC 29464

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1 Call for action: Paradigm shift in teaching microbiology in a community colleges Kaustubha Qanungo Ph.D Biological Sciences Trident Technical College 7000 Rivers Avenue Charleston SC 29464

2 Project Course: BIO 225 Microbiology in a 7.5 week compressed format In class activity Time: 3 hours SAT/SUN MON TUE Out of class mandatory activity Time: 3 hours Drive them crazy again

3 BIO225 Microbiology Objectives BIO225: BIO225: Microbial Genetics of infection objectives: Students will 1. Be able to describe components and structure of the DNA and RNA 2. Be able to describe the components of the Central Dogma 3. Predict how mutations impact the Central Dogma 4. Describe how bacteria exchange genetic material 5. Be able to read a popular article on drug resistance and explain the mechanism.

4 Project Course: BIO 225 Microbiology in a 7.5 week compressed format SAT/SUN MON 1 Formative assessment 2 Summative assessment

5 Project Course: BIO 225 Microbiology in a 7.5 week compressed format SAT/SUN MON 1. Watch video lecture with embedded non-graded quiz with hints after a wrong answer. Video resumes after answering correct. 2

6 Component 1: Video lecture with embedded questions Question: Why each strand of a double strand DNA molecule is antiparallel to each other? A. The phosphate groups are needed to be in opposite orientation to bond with phosphate group of the complementary strand. B. The strands are complimentary to each other so they needed to be antiparallel C. The bases of the nucleotide are aligned in a complementary fashion and the bonding with the complementary base pairs are only possible if the strands are antiparallel. D. The DNA strands are antiparallel because it contains thymine instead of uracil.

7 Project Course: BIO 225 Microbiology in a 7.5 week compressed format SAT/SUN MON 1. Watch video lecture with embedded non-graded quiz with hints after a wrong answer. Video resumes after correct answer. 2. Take online warm up quiz with lockdown browser.

8 Component 2: Warm up quiz Question: The following is a DNA sequence 5`ATGCGATTCCATTAG3`. Predict the complementary strand of the DNA shown. A. 5`ATGCGATTCCATTAG3 B. 3`ATGCGATTCCATTAG5` C. 5`TACGCTAAGGTAATC3` D. 3`TACGCTAAGGTAATC5`

9 Project Course: BIO 225 Microbiology in a 7.5 week compressed format SAT/SUN 1. Watch video lecture with embedded non-graded quiz with hints after a wrong answer. Video resumes after correct answer. 2. Read Text and Lecture outline in D2L. Take online warm up quiz with lockdown browser. 3. Selected difficult topic lecture with graded clicker questions. 4 MON 5

10 Component 3: In Class Lecture with Clicker Question Question: Why the DNA polymerase synthesize the 3`-5` strand discontinuously? A. Because each DNA polymerase is too big of a molecule and need to be far apart from each other B. The DNA polymerase can only synthesize DNA in the 5`-3` direction which is the complimentary strand of the 3`-5`. C. As DNA replication is semi-conservative, the DNA polymerase need to wait until the 5`-3` strand is synthesized. D. The 3`-5` strand of the DNA is synthesized discontinuously because it allows the tired DNA polymerase to rest in-between so that it does not cause mistake leading to mutations

11 Project Course: BIO 225 Microbiology in a 7.5 week compressed format 3. Selected difficult topic lecture with graded clicker questions. 4. Group assignment follweed by individual grading. 5 SAT/SUN MON 1. Watch video lecture with embedded non-graded quiz with hints after a wrong answer. Video resumes after correct answer. 2. Take online warm up quiz with lockdown browser.

12 Component 4: Group activity; Literature study, writing Question: Explain how the CRE (Carbapenam resistance bacteria) bacteria acquired the ability to resist the action of the Carbapenam?

13 Project Course: BIO 225 Microbiology in a 7.5 week compressed format 3. Selected difficult topic lecture with graded clicker questions. 4. Group assignment with group and individual peer grading. 5. Interactive class session to revisit topics and quiz. SAT/SUN MON 1. Watch video lecture with embedded non-graded quiz with hints after a wrong answer. Video resumes after correct answer. 2. Take online warm up quiz with lockdown browser.

14 Component 5: Closing lecture and summative quiz 1. Summary and closing lecture for 30 minute. 2. Encourage strong participation to revisit muddy areas. 3. Summative multiple choice quiz worth 20 points. Question: The processes of the transcription and translation are similar in that they both. A. Use DNA to produce their respective product B. Produce mrna C. Have a initiation, elongation and termination D. Require Amino Acyl trna E. Take place in the nucleus

15

16 The Written Plan Project Course: BIO 225 Microbiology in a 7.5 week compressed format Project Chapter: Microbial genetics of infectious disease (Molecular Biology)

17 Component 1: Video lecture with embedded questions 1. Delivered thru Desire2Learn (D2L). 2. Hybrid video lecture: Voice over powerpoint with the face of the instructor in a corner window 3. Scripted with close captioning 4. Embedded non-graded critical thinking quizzes; wrong answer will provide some hint for correct answer. 5. Video resumes after correct answer Question: Why each strand of a double strand DNA molecule is antiparallel to each other? A. The phosphate groups are needed to be in opposite orientation to bond with phosphate group of the complementary strand. B. The strands are complimentary to each other so they needed to be antiparallel C. The bases of the nucleotide are aligned in a complementary fashion and the bonding with the complementary base pairs are only possible if the strands are antiparallel. D. The DNA strands are antiparallel because it contains thymine instead of uracil.

18 Component 2: Warm up quiz 1. Students will read the assigned chapter from the text book. 2. They will take an online 10 point quiz which asks fundamental concepts from that chapter 3. The quiz will be through D2L and the lockdown browser. 4. One question shown at a time. 5. Students have two chances to take the quiz. 6. The software will choose question random questions from a question bank. Question: The following is a sequence of a DNA 5`ATGCGATTCCATTAG3`. complementary strand of that DNA. A. 5`ATGCGATTCCATTAG3 B. 3`ATGCGATTCCATTAG5` C. 5`TACGCTAAGGTAATC3` D. 3`TACGCTAAGGTAATC5`

19 Component 3: In Class Lecture with Clicker Question 1. Conventional class lecture with Powerpoint. 2. Embedded animations. 3. Embedded videos 4. The lecture part will include 5 point clicker question. Question: Why the DNA polymerase synthesize the 3`-5` strand discontinuously? A. Because each DNA polymerase is too big of a molecule and need to be far apart from each other B. The DNA polymerase can only synthesize DNA in the 5`-3` direction which is the complimentary strand of the 3`-5`. C. As DNA replication is semi-conservative, the DNA polymerase need to wait until the 5`-3` strand is synthesized. D. The 3`-5` strand of the DNA is synthesized discontinuously because it allows the tired DNA polymerase to rest in-between so that it does not cause mistake leading to mutations

20 Component 4: Group activity 1. Heterogeneous student groups will be formed based on their academic ability. Decided at the beginning of the semester. There will be four students per group. 2. A popular science literature (Scientific American level) on antibiotic resistance will be provided in the print form to each student. 3. They will read the material and discuss the problem for 45 minutes. 4. Then they will write their answers in 15 minutes which will be graded. 5. There will be 10 points on this activity and will be graded based on a rubric Question: Explain how the CRE (Carbapenam resistance bacteria) bacteria acquired the ability to resist the action of the Carbapenam in the light of Central Dogma?

21 Component 5: Interactive revisit lecture and summative quiz 1. Summary and closing lecture for 30 minute. 2. Encourage strong participation to revisit muddy areas. 3. Summative multiple choice quiz worth 20 points. Question: The processes of the transcription and translation are similar in that they both. A. Use DNA to produce their respective product B. Produce mrna C. Have a initiation, elongation and termination D. Require Amino Acyl trna E. Take place in the nucleus

22 Project Course: BIO 225 Microbiology in a 7.5 week compressed format Course Evaluation 1. Online quiz 10 points 2. Clicker questions 10 points 3. Group assignment points 10 points 4. In class quiz 20 points Total point per chapter (Drop worst one) 50 points X (15-1) 700 points Lecture test X 4 50 points 200 points Final test Course total 100 points 1000 points

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