Producción in vitro de embriones 313 PRODUCCIÓN IN VITRO DE EMBRIONES BOVINOS

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1 Producción in vitro de embriones 313 PRODUCCIÓN IN VITRO DE EMBRIONES BOVINOS Gustavo A Palma Introducción La producción in vitro de embriones es el procedimiento para producir embriones en 3 etapas, la maduración in vitro (MIV) de los ovocitos, la fecundación in vitro (FIV) y el cultivo in vitro (CIV) de los posibles cigotos, Figura 1. El desarrollo in vitro de los ovocitos está íntimamente relacionado con la aptitud que adquieren durante su etapa folicular previa. Por ello, para una mayor comprensión de los eventos que ocurren in vitro y de los factores que los afectan fue desarrollado el capítulo de desarrollo folicular temprano (ver capítulo en este libro).

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19 Producción in vitro de embriones 331 Sánchez R, Concha M, Ichikawa T, Henkel R and WM Schill (1996) Glass wool filtration reduces reactive oxygen species by elimination of leukocytes in oligozoospermic patients with leukocytospermia J Assisted Reprod Genet 13, Sardet C, Dumollard R, A McDougall (2006) Signals and Calcium waves at fertilization Sem Cell Devel Biol 17, Sato E, Matsuo M, H Miyamoto (1990) Meiotic maturation of bovine oocytes in vitro: improvement of meiotic competence by dibutyryl ciclic adenosine 3,5 -monophosphate J Anim Sci 68, Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, FA Lai (2002) PLC: a sperm-specific trigger of Ca2+ oscillation in eggs and embryo development Development 129, Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, FA Lai (2002) PLC: a sperm-specific trigger of Ca2+ oscillation in eggs and embryo development Development 129, Scherntahner W, Schmoll F, Brem G, K Schellender (1997) Storing bovine ovaries for 24 hours between 15 and 21 C does not influence in vitro production of blastocysts Theriogenology 47, 297 Schernthaner W, Wenigerkind H, Stojkovic M, Palma GA, Modl J, Wolf E, G Brem (1999) Pregnancy rate after ultrasound-guided follicle aspiration in nonlactating cows from different breeds Zentralbl Veterinarmed A 46, 33-7 Shimasaki S, Moore RK, Otsuka F, GF Erickson (2004) The bone morphogenetic protein system in mammalian reproduction Endocr Rev Schultz GA, A Hogan, A Watson, Smith RM and S Heyner (1992) Insulin-like growth factors and glucose transporters temporal patterns of gene expression in early murine and bovine embryos Reprod Fert Dev 4, Schultz RM (1987) Molecular aspects of oocyte growth and maturation, In: Experimental approaches to mammalian embryonic development Univ Press, Cambridge Schultz RM (2005) From egg to embryo: a peripatetic journey Reproduction 130, Schultze D, B Brunswig, AK Mukhpadhyay (1989) Renin and prorenin-like activities in bovine ovarian follicles Endocrinol 124, Schwartz J, MR Schneider, JL Rodrigues and H-D Reichenbach (1998) Effect of short-term storage of bovine oocytes in different media and temperatures on the subsequent in vitro embryo development Theriogenology 49, 217 Schweitzer CM, Gonzalvez PBD, Neves JP, Severo NC, morales CN, AG Hernández (1996) Influencia deo percoll na capacitacao espermática para fecundacao in vitro de ooctos bovinos XI Congreso brasilero de reproduçao animal, Belo horizonte, Minas Gerais, 275 Shamsuddin M (1993) Effect of growth factors on bovine blastocyst development in a serum-free medium PhD Thesis, Swedish University of Agricultural Sciences Sweden, pp 1-60 Shamsuddin M and B Larsson (1993) In vitro development of bovine embryos after fertilization using semen from different donors Reprod Dom Anim 28, Shamsuddin M, Larsson B, Gustafsson H and H Rodriguez-Martinez (1993) A serum-free, cell culture system for development of bovine one-cell embryos up to blastocist stage with improve viability Doctoral Thesis, Univ Of Uppsala, Sweden V, 1-12 Sharma RK and A (1996) Agarwal Role of reactive oxygen species in male infertility Urology 48, Shimasaki S, Moore, RK, Otsuka, F and GF Erickson (2004) The bone morphogenetic protein system in mammalian reproduction Endocr Rev 25, Sikka SC (2004) Role of oxidative stress and antioxidants in andrology and assisted reproductive technology J Androl 25, 5-18 Simmen RCM, Y Ko, FA Simmen (1993) Insulin-like growth factors and blastocyst development Theriogenology 39, Sinclair KD, Broadbent PJ and DF Dolman (1995) In vitro produced embryos as a means of achieving pregnancy and improving productivity in beef cows Anim Sci 60, Sirard MA and P Blondin (1996) Oocyte maturation and IVF in cattle An Reprod Sci Sirard MA, Coenen, K (1993) The co-culture of cumulus enclosed bovine oocytes and hemisections of follicles, effects on meiotic resumption Theriogenology 40, Sirard MA, Richard F and M Mayes (1998) Controlling meiotic resumption in bovine oocytes: a review Theriogenology 49, Sirard MA, Richard F, Blondin P, C Robert (2006) Contribution of the oocyte to embryo quality Theriogenology 7,

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