German Society of Immunology Study Group Biology of B Lymphocytes. 13 th B Cell Forum. February 19-21, Hitzacker, Elbe

Transcription

1 German Society of Immunology Study Group Biology of B Lymphocytes 13 th B Cell Forum February 19-21, 2015 Hitzacker, Elbe

2 CONTENT Program/General Information... 1 Program... 2 Titles Poster Session... 7 Participants Abstracts - Oral Presentations Session I: B cell memory, germinal centers and T follicular helper cells. 15 Session IIa: B cell development, signaling and activation Session IIb: B cell development, signaling and activation Session III: B cell ontogeny and dynamics of B cell homeostasis Session IVa: Plasma cells, antibody production and autoimmunity Session IVb: Plasma cells, antibody production and autoimmunity Abstracts - Poster Presentations B cell memory, germinal centers and T follicular helper cells Methods B cell development, signaling and activation B cell ontogeny and dynamics of B cell homeostasis Plasma cells, antibody production and inflammation Sponsors... 96

3 PROGRAM/GENERAL INFORMATION MEETING SCHEDULE February, 19: Registration will start at 15:00 February 21: Farewell Lunch / Departure at 11:30 ORAL PRESENTATIONS All talks will be 10 minutes plus 5 minutes discussion (exception: keynote speakers) Please hand in your PowerPoint presentation on a USB stick before or during the break before your session. Sorry, no personal laptops can be used for presentation. POSTER PRESENTATIONS Posters should be printed in size A0 Posters should be mounted on February 19 between 15:00 and 19:00 Poster presentation will begin on February 20 at 18:30 1

4 PROGRAM Thursday, February 19, :00 19:00 Registration and Poster Setup 15:30 16:50 Get-together (coffee and cake) 16:55 Welcome (Jürgen Wienands) Session I: B cell memory, germinal centers and T follicular helper cells Chair: Thomas Winkler / Hyun-Dong Chang 17:00 Andreas Hutloff, Berlin ICOS maintains the T follicular helper cell phenotype by downregulating Krüppel-like factor 2 17:15 Michael Meyer-Hermann, Braunschweig Overcoming the dichotomy of quantity and quality of germinal centre output 17:30 Thomas Winkler, Erlangen Adoptive immunotherapy with memory B cells - A Phase I/IIa clinical trial for patients after hematopoietic stem cell transplantation 17:45 18:10 Break Keynote Lecture Chair: Rudolf Manz 18:10 19:10 Surprise Speaker 19:30 Dinner (Buffet) 2

5 Friday, February 20, :00 8:45 Breakfast Session IIa: B cell development, signaling and activation Chair: Jürgen Wienands / Ari Waisman 8:45 Jianying Yang, Freiburg The dissociation activation model of B cell activation 9:00 Dirk Mielenz, Erlangen Quantification of surface receptor clustering by flow cytometry 9:15 Bernd Schröder, Kiel Processing of CD74 by the intramembrane protease SPPL2a is critical for PI3K/Akt signaling in developing B cells 9:30 Christian Kosan, Jena The transcription factor 'Myc interacting zinc finger protein 1' is a key regulator for B cell development and activation 9:45 Kristin Schröder, Lübeck The anaphylatoxin C5a drives pathogenic IgG autoantibody formation in an active model of experimental epidermolysis bullosa acquisita 10:00 Coffee Break 3

6 Session IIb: B cell development, signaling and activation Chair: Andreas Radbruch / Rudi Hendriks 10:30 Edgar Serfling, Würzburg The Transcription Factor NFATc1 Affects the Fate of Peripheral B Cells by Controlling their Aerobic Glycolysis 10:45 Glen Pearce, Dresden The Role of SWAP70 in DNA Repair and Cancer 11:00 Alexander Weber, Tübingen Oncogenic MyD88 mutations in B cell malignancies 11:15 Jürgen Wienands, Göttingen Absence of CIN85 expression presents with X-chromosome-linked antibody deficiency in humans 11:30 Nadine Hövelmeyer, Mainz Over-Expression of an Alternative Splice Variant of the Negative NFkappaB Regulator CYLD Leads to the Development of B-CLL in Mice. 12:00 Lunch Session III: B cell ontogeny and dynamics of B cell homeostasis Chair: Edgar Meinl / Lars Nitschke 14:00 Franziska Fettke, Magdeburg The effect of pregnancy hormones and trophoblastderived factors on IL-10 producing regulatory B cells 14:15 Alice Muggen, Rotterdam Dynamics in B cell subset and B cell repertoire distribution during human aging 14:30 Martina Przekopowitz, Essen Ontogeny, differentiation and function of human mature CD5+ B cells 14:45 Katharina Bröker, Lübeck Non-classical complement activation regulates the peritoneal B1 cell compartment 15:00 Martin Lipp, Berlin The transcriptional coactivator Bob1 regulates the development of TFH cells 4

7 15:30 17:30 Elbe /Hitzacker Walk 17:30 Meeting Study Group Biology of B Lymphocytes 18:30 Poster Session and Dinner (Buffet) 20:45 Concession of poster award 21:00 Party 5

8 Saturday, February 21, :00 8:45 Breakfast Session IVa: Plasma cells, antibody production and autoimmunity Chair: Hans-Martin Jäck / Andreas Hutloff 8:45 Edgar Meinl, München The immunoregulator soluble TACI is released by ADAM10 and reflects B-cell activation in autoimmunity 9:00 Odilia Corneth, Rotterdam Dysregulated Btk expression in peripheral blood B lymphocytes in systemic autoimmune disease 9:15 Simon Eschweiler, Lübeck Development of differentially glycosylated antibodies 9:30 Hans-Martin Jäck, Erlangen How MicroRNAs Control Plasma Cells 9:45 Break Session IVb: Plasma cells, antibody production and autoimmunity Chair: Falk Nimmerjahn / Claudia Berek 10:15 Stefanie Schmidt, Berlin Towards a synthetic niche for memory plasma cells 10:30 Britta Frehse, Lübeck Increased c-mpl dependent megakaryopiesis controls plasma cell formation in lupus 10:45 Claudia Berek, Berlin Eosinophils promote the generation and maintenance of IgA plasma cells 11:30 13:00 Farewell Lunch /Departure 6

9 TITLES POSTER SESSION 1. Caren Bartsch, Göttingen Regulation of intracellular B cell antigen receptor signalling events by a recombinant soluble FcγIIB receptor 2. Alexander Beller, Berlin Development and survival of IgA + plasma cells in peyer s patches of eosinophil-deficient mice 3. Julia Bischof, Lübeck Establishment of an infrastructure for the analysis of the B cell immune repertoire: application to the analysis of B cell subpopulations in auto-immune blistering dermatoses 4. Kathrin Brenker, Freiburg Photo-Caging of the Spleen Tyrosine Kinase 5. Katharina Bröker, Lübeck Non-classical complement activation regulates the peritoneal B1 cell compartment 6. Eva-Maria Cox, Mainz B cell specific overexpression of Akt leads to expansion of marginal zone B cells 7. Marjolein de Bruijn, Rotterdam Regulatory role for Tec Kinase in B cell immunity 8. Kai Dittman, Göttingen mb1-cre-controlled ablation of Shp2 (Ptpn11) causes metachondromatosis 9. Asmaa El Beidaq, Lübeck Can diet affect the B cell receptor repertoire diversity in mice? 10. Niklas Engels, Göttingen The extracellular membrane-proximal domain of membrane-bound IgE restricts surface expression and signaling of mige-bcrs 7

10 11. Simon Eschweiler, Lübeck The development of pro-inflammatory agalactosylated IgG antibodies is dependent on the pro-inflammatory cytokines IFN-γ and IL Stephanie Gläsener, Hannover Human neonatal B cell subpopulations are intrinsically immature 13. Matthias Hahn, Mainz Over-expression of an alternative splice variant of the negative NFκB regulator CYLD leads to the development of B-CLL in mice 14. Juliane Hobusch, Lübeck Allergen-specific immunotherapy (SIT) induce sialylated immunesuppressive IgG antibodies 15. Bimba Hoyer, Berlin Differential B and plasma cell homing mechanisms in inflamed kidneys of NZB/W F1 mice 16. Andreas Hutloff, Berlin Local T/B cooperation in inflamed tissues is supported by a unique population of B helper T cells distinct from T follicular helper cells 17. Kathrin Kläsener, Freiburg B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk 18. Alexei Leliavski, Lübeck Identifying molecular pathways that govern the development of tolerogenic plasma cells in vivo 19. Christopher Link, Lübeck The impact of IL-10 on IgE-mediated food allergy 20. Anna Lollies, Essen The AP-1 transcription factor BATF3 is aberrantly expressed in classical Hodgkin lymphoma and contributes to tumor cell survival and proliferation 21. Dirk Mielenz, Erlangen Quantification of surface receptor clustering by flow cytometry 8

11 22. Lavanya Mokada Gopal, Erlangen Functional characterization of STAT6-regulated genes in B cells and their impact on the Germinal Centre response 23. Alice Muggen, Rotterdam Dynamics in B cell subset and B cell repertoire distribution during human aging 24. Zane Orinska, Borstel CD37-specific monoclonal antibodies as a tool to target B cells 25. Lamia Özgör, Erlangen Effects of Epratuzumab, a monoclonal antibody targeting human CD22, on B cell function in human CD22 knock-in mice 26. Katharina Pracht, Erlangen Function of mir-148a in mature plasma cells 27. Dorothea Reimer, Erlangen Regulation of Lymphocyte Adhesion and Migration by Swiprosin-1/ EFhd2 28. Marta Rizzi, Freiburg Treatment with tofacitinib alters human B lymphocyte development and function 29. Julia Schmid, Erlangen mir-148a a key regulator in plasma cell differentiation? 30. Stefanie Schmidt, Berlin Towards a synthetic niche for memory plasma cells 31. Maren Schmidt, Göttingen Global transcriptional amplification by Myc in B lymphocytes: an assay to detect selective up- and downregulation of specific Myc target genes. 32. Verena Schmitt, Erlangen IL-36α is expressed in B lymphocytes and activates synovial like fibroblasts 9

12 33. Heike Schmitt, Erlangen The role of Siglec-H in autoimmunity and during infection 34. Kristin Schröder, Lübeck The anaphylatoxin C5a drives pathogenic IgG auto-antibody formation in an active model of experimental epidermolysis bullosa acquisita 35. Wolfgang Schuh, Erlangen The role of KLF2 and KLF4 in generation, homing and survival of plasma cells in vitro and in vivo 36. Kathrin Schulz, Göttingen Absence of CIN85 expression presents with X-chromosome-linked antibody deficiency in humans 37. Simar Pal Singh, Rotterdam Characterization of novel mouse cell lines to study the role of B-cell receptor signaling in development of Chronic Lymphocytic Leukemia 38. Merle Stein, Erlangen Function of the protein Swiprosin-2/EFhd1 in early B lymphocyte development 39. Sandra Tretow, Berlin Molecular interference of vitamin A and D receptors in B lymphocytes 10

13 PARTICIPANTS NAME CITY ORGANISATION Bartsch Caren Göttingen University Hospital Göttingen Beller Alexander Berlin DRFZ Berlin Berek Claudia Berlin DRFZ Berlin Bischof Julia Lübeck University of Lübeck Brandl Carolin Erlangen FAU Erlangen - Nürnberg carolin.brandl@fau.de Brenker Kathrin Freiburg MPI of Immunology and Epigenetics kathrin.brenker@gmail.com Bröker Katharina Lübeck University of Lübeck Katharina.Broeker@uksh.de Cavallari Marco Freiburg University of Freiburg Chang Hyun-Dong Freiburg DRFZ Berlin chang@drfz.de Corneth Odilia Rotterdam Erasmus MC Rotterdam o.corneth@erasmusmc.nl Cox Eva-Maria Mainz University of Mainz cox@uni-mainz.de de Bruijn Marjolein Rotterdam Erasmus MC Rotterdam j.w.debruijn@erasmusmc.nl Dittmann Kai Göttingen University of Göttingen kdittma@gwdg.de Ehlers Marc Lübeck University of Lübeck marc.ehlers@uk-sh.de El Beidaq Asmaa Lübeck University of Lübeck asmaaelbeidaq@gmail.com Engelke Michael Göttingen University of Göttingen mengelk@gwdg.de Engels Niklas Göttingen University of Göttingen nengels@gwdg.de Eschweiler Simon Lübeck University of Lübeck Simon.Eschweiler@uksh.de Fettke Franziska Magdeburg Otto-von-Guericke University franziska.fettke@med.ovgu.de Figge Julia Lübeck University of Lübeck julia.figge@uksh.de Frehse Britta Lübeck University of Lübeck Britta.Frehse@yahoo.com Gläsener Stephanie Hannover Hannover Medical School Hahn Matthias Mainz University of Mainz hahn002@uni-mainz.de Heine Guido Berlin University Hospital Charité guido.heine@charite.de Hendriks Rudi Rotterdam Erasmus MC Rotterdam r.hendriks@erasmusmc.nl Hitzing Christoffer Göttingen University Hospital Göttingen C.Hitzing@googl .com Hobusch Juliane Lübeck University of Lübeck juliane.hobusch@uksh.de Hofmann Katharina Lübeck University of Lübeck katharina.hofmann@outlook.com Hövelmeyer Nadine Mainz University of Mainz hoevelme@uni-mainz.de Hoyer Bimba Berlin University Hospital Charité hoyer@drfz.de Hutloff Andreas Berlin DRFZ Berlin hutloff@drfz.de Jäck Hans-Martin Erlangen FAU Erlangen - Nürnberg hjaeck@gmail.com Jessberger Rolf Dresden TU Dresden rolf.jessberger@tu-dresden.de Karsten Christian Lübeck University of Lübeck christian.karsten@uksh.de Kläsener Kathrin Freiburg University of Freiburg caren.bartsch@med.unigoettingen.de marco.cavallari@bioss.unifreiburg.de Glaesener.Stephanie@mhhannover.de kathrin.klaesener@bioss.unifreiburg.de Klopp Kirstin Biozol Biozol Diagnostica K.klopp@biozol.de Kosan Christian Jena Friedrich-Schiller-University Jena christian.kosan@uni-jena.de Kube Dieter Göttingen Universitätsmedizin Göttingen dkube@med.uni-goettingen.de Küppers Ralf Essen University of Duisburg-Essen ralf.kueppers@uk-essen.de Lahmann Annette Berlin DRFZ Berlin annette.lahmann@drfz.de 11

14 NAME CITY ORGANISATION Langhans Kristina Borstel Research Center Borstel Leliavski Alexei Lübeck University of Lübeck Leypoldt Frank Kiel UKSH Kiel Lilienthal Gina-Maria Lübeck University of Lübeck Link Christopher Lübeck University of Lübeck Lipp Martin Berlin Max-Delbrück-Centrum Berlin Lollies Anna Essen University Hospital Essen Manz Rudolf Lübeck University of Lübeck Meinl Edgar München LMU München Meng Lingzhang Lübeck University of Lübeck Meyer- Hermann Michael Braunschweig HZI Mielenz Dirk Erlangen FAU Erlangen - Nürnberg dmielenz@molmed.uni-erlangen.de Mokada Gopal Lavanya Erlangen FAU Erlangen lavanya.lyla@gmail.com Muggen Alice Rotterdam Erasmus MC Rotterdam a.muggen@erasmusmc.nl Nimmerjahn Falk Erlangen FAU Erlangen - Nürnberg falk.nimmerjahn@fau.de Nitschke Lars Erlangen FAU Erlangen - Nürnberg lars.nitschke@fau.de Orinska Zane Borstel Research Center Borstel zorinska@fz-borstel.de Özgör Lamia Erlangen FAU Erlangen - Nürnberg Lamia.Oezgoer@fau.de Pearce Glen Dresden TU Dresden glen.pearce@tu-dresden.de Petry Janina Lübeck University of Lübeck janina.petry@web.de Piedavent- Salomon Melanie Sony Sony Europe Limited Pillai Saravanan Rotterdam Erasmus MC Rotterdam s.yuvaraj@erasmusmc.nl Pracht Katharina Erlangen University Hospital Erlangen katharina.pracht@freenet.de Przekopowitz Martina Essen University Duisburg - Essen Radbruch Andreas Berlin DRFZ Berlin radbruch@drfz.de Rahmöller Johann Lübeck University of Lübeck johann.rahmoeller@gmail.com Reimer Dorothea Erlangen University Hospital Erlangen dreimer@molmed.uni-erlangen.de Reth Michael Freiburg Albert-Ludwigs-University Freiburg michael.reth@bioss.uni-freiburg.de, officer@ie-freiburg.mpg.de Rizzi Marta Freiburg University Hospital Freiburg marta.rizzi@uniklinik-freiburg.de Schepp Johanna Freiburg Albert-Ludwigs-University Freiburg Schmid Julia Erlangen University Hopsital Erlangen jschmid@molmed.uni-erlangen.de Schmidt Stefanie Berlin DRFZ Berlin s.schmidt@drfz.de Schmidt Maren Göttingen University of Göttingen Schmitt Verena Erlangen FAU Erlangen - Nürnberg verena.schmitt@uk-erlangen.de Schmitt Heike Erlangen FAU Erlangen - Nürnberg heike.schmitt@fau.de Schmücker Anne Preprotech Peprotech GmbH aschmuecker@peprotech.de Schramm Gabriele Borstel Research Center Borstel gschramm@fz-borstel.de Schröder Kristin Lübeck University of Lübeck kristin.schroeder@uksh.de Schröder Bernd Kiel Christian-Albrechts-University Kiel Schubert Desirée Freiburg University Hospital Freiburg baschroeder@biochem.uni-kiel.de melanie.piedaventsalomon@eu.sony.com martina.przekopowitz@stud.unidue.de johanna.schepp@uniklinikfreiburg.de maren.schmidt@med.unigoettingen.de desiree.schubert@uniklinikfreiburg.de 12

15 NAME CITY ORGANISATION Schuh Wolfgang Erlangen FAU Erlangen - Nürnberg wschuh@molmed.uni-erlangen.de Schulz Kathrin Göttingen University of Göttingen kathrin.schulz@med.unigoettingen.de Schumacher Anne Magdeburg Otto-von-Guericke University anne.schumacher@med.ovgu.de Serfling Edgar Wuerzburg University of Würzburg serfling.e@mail.uni-wuerzburg.de Singh Simar Pal Rotterdam Erasmus MC Rotterdam s.palsingh@erasmusmc.nl Stein Merle Erlangen University Hospital Erlangen mstein@molmed.uni-erlangen.de Tiburzy Benjamin Biolegend Biolegend btiburzy@biolegend.com Treptow Sandra Berlin TU Berlin sandra.treptow@charite.de Waisman Ari Mainz University of Mainz waisman@uni-mainz.de Weber Alexander Tübingen University of Tübingen alexander.weber@uni-tuebingen.de Weiss Siegfried Braunschweig HZI siegfried.weiss@helmholtz-hzi.de Wienands Jürgen Göttingen University of Göttingen jwienan@gwdg.de Winkler Thomas Erlangen FAU Erlangen - Nürnberg thomas.winkler@fau.de Wolfram Udo Milteny Miltenyi Biotec bettinar@miltenyibiotec.de Yang Jianying Freiburg MPI of Immunology and Epigenetics yang@immunbio.mpg.de Zenclussen Ana Magdeburg Otto-von-Guericke University ana.zenclussen@med.ovgu.de 13

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