Amino Acids, Proteins, and Enzymes. Primary and Secondary Structure Tertiary and Quaternary Structure Protein Hydrolysis and Denaturation

Transcription

1 Amino Acids, Proteins, and Enzymes Primary and Secondary Structure Tertiary and Quaternary Structure Protein Hydrolysis and Denaturation 1

2 Primary Structure of Proteins H 3 N The particular sequence of amino acids that is the backbone of a peptide chain or protein CH 3 O+ CH C N H CH 3 CH CH 3 CH CH O C N H SH CH 2 CH O C Ala-Leu-Cys-Met N H CH 3 S CH 2 CH 2 O CH C O - 2

3 Secondary Structure Alpha Helix Three-dimensional arrangement of amino acids with the polypeptide chain in a corkscrew shape Held by H bonds between the H of N-H group and the O of C=O of the fourth amino acid along the chain Looks like a coiled telephone cord 3

4 Secondary Structure Beta Pleated Sheet Polypeptide chains are arranged side by side Hydrogen bonds form between chains R groups of extend above and below the sheet Typical of fibrous proteins such as silk 4

5 Secondary Structure Triple Helix Three polypeptide chains woven together Glycine, proline, hydroxy proline and hydroxylysine H bonding between OH groups gives a strong structure Typical of collagen, connective tissue, skin, tendons, and cartilage 5

6 Learning Check P1 Indicate the type of structure as (1) primary (2) alpha helix (3) beta pleated sheet (4) triple helix A. Polypeptide chain held side by side by H bonds B. Sequence of amino acids in a polypeptide chain C. Corkscrew shape with H bonds between amino acids D. Three peptide chains woven like a rope 6

7 Solution P1 Indicate the type of structure as (1) primary (2) alpha helix (3) beta pleated sheet (4) triple helix A. 3 Polypeptide chain held side by side by H bonds B. 1 Sequence of amino acids in a polypeptide chain C. 2 Corkscrew shape with H bonds between amino acids D. 4 Three peptide chains woven like a rope 7

8 Tertiary Structure Specific overall shape of a protein Cross links between R groups of amino acids in chain disulfide S S + ionic COO H 3 N H bonds C=O HO hydrophobic CH 3 H 3 C 8

9 Learning Check P2 Select the type of tertiary interaction as (1) disulfide (2) ionic (3) H bonds (4) hydrophobic A. Leucine and valine B. Two cysteines C. Aspartic acid and lysine D. Serine and threonine 9

10 Solution P2 Select the type of tertiary interaction as (1) disulfide (2) ionic (3) H bonds (4) hydrophobic A. 4 Leucine and valine B. 1 Two cysteines C. 2 Aspartic acid and lysine D. 3 Serine and threonine 10

11 Globular and Fibrous Proteins Globular proteins spherical shape Insulin Hemoglobin Enzymes Antibodies Fibrous proteins long, thin fibers Hair Wool Skin Nails 11

12 Quaternary Structure Proteins with two or more chains Example is hemoglobin Carries oxygen in blood Four polypeptide chains Each chain has a heme group to bind oxygen 12

13 Learning Check P3 Identify the level of protein structure 1. Primary 2. Secondary 3. Tertiary 4. Quaternary A. Beta pleated sheet B. Order of amino acids in a protein C. A protein with two or more peptide chains D. The shape of a globular protein E. Disulfide bonds between R groups 13

14 Solution P3 Identify the level of protein structure 1. Primary 2. Secondary 3. Tertiary 4. Quaternary A. 2 Beta pleated sheet B. 1 Order of amino acids in a protein C. 4 A protein with two or more peptide chains D. 3 The shape of a globular protein E. 3 Disulfide bonds between R groups 14

15 Protein Hydrolysis Break down of peptide bonds Requires acid or base, water and heat Gives smaller peptides and amino acids Similar to digestion of proteins using enzymes Occurs in cells to provide amino acids to synthesize other proteins and tissues 15

16 Hydrolysis of a Dipeptide + H 3 N CH 3 O CH C + H 3 N N H OH CH 2 CH O C CH 3 O CH COH OH H 2 O, H + heat OH + CH 2 O + H 3 N CH C OH 16

17 Denaturation Disruption of secondary, tertiary and quaternary protein structure by heat/organics Break apart H bonds and disrupt hydrophobic attractions acids/ bases Break H bonds between polar R groups and ionic bonds heavy metal ions React with S-S bonds to form solids agitation 17 Stretches chains until bonds break

18 Applications of Denaturation Hard boiling an egg Wiping the skin with alcohol swab for injection Cooking food to destroy E. coli. Heat used to cauterize blood vessels Autoclave sterilizes instruments Milk is heated to make yogurt 18

19 Learning Check P4 What are the products of the complete hydrolysis of Ala-Ser-Val? 19

20 Solution P4 The products of the complete hydrolysis of Ala-Ser-Val are alanine serine valine 20

21 Learning Check P5 Tannic acid is used to form a scab on a burn. An egg becomes hard boiled when placed in hot water. What is similar about these two events? 21

22 Solution P5 Acid and heat cause a denaturation of protein. They both break bonds in the secondary and tertiary structure of protein. 22