INS TITU TE O F IM M U NO LO GY AND
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1 INS TITU TE O F IM M U NO LO GY AND TR ANS FU S IO N M E D ICINE
2 FACTS AND FIGURES Ins titute of Im m unology and Trans fus ion M e dicine D e partm e nt of Im m unology Fe rdinand- Saue rbruch - Straße Gre ifs w ald Te le ph one : Te le fax: E-m ail: H om e page : s ch ue tt@ uni- gre ifs w ald.de h ttp://w w w.m e dizin.uni- gre ifs w ald.de /im m un/ H e ad of ins titute : Univ.- Prof. D r. m e d. C. Sch ütt M patie nts. M ED ICAL CARE SERVICES e dicalcare s e rvice s offe re d by th e de partm e nt of im m unology include a l laboratory m e th ods ne ce s s ary for com pre h e ns ive im m unodiagnos e s of Th is include s : Im m unoph e notyping Th is proce dure is pe rform e d w ith th e h e lp of FACS analys e s of pe riph e ralblood, bone m arrow, bronch oalve olar lavage and puncture m ate rials of oth e r body fluids (s ynovial fluid, liq uor ce re bros pinalis, e ffus ion m ate rialfrom ple ura or as cite s ). By us ing m ore th an 50 diffe re nt m onoclonalantibodie s th e e xam ination e nable s us to ide ntify a large num be r of diffe re nt ce lular s ubcate gorie s (h ttp://w w w.m e dizin.uni- gre ifs w ald.de / im m un/k linim m u). QUALITY REPORT FOR PATIENTS 2006 UNIVERSITY CLINIC GREIFSW ALD
3 Im portant and ofte n pe rform e d proce dure s are q uantifications of lym ph ocyte s ubpopulations in pe riph e ralblood (s o ca le d im m une s tatus ), im m unoph e notyping of le uk ae m ia ce ls or lym ph om a ce ls, im puls e cytoph otom e tric M RD diagnos e s, applie d for e xam ple to diagnos e a pos s ible m inim alre s idualaffe ction to controlth e s ucce s s of le uk ae m ia or lym ph om a tre atm e nts, and th e q uantification of m onocytal H LA- D R- e xpre s s ion de ns ity to ide ntify acute im m unode ficie ncy. Functionalce ldiagnos tics W ith in im m unode ficie ncy diagnos is e s s e ntialce lfunctions are te s te d by in- vitro te s ts : lym ph ocyte trans form ation te s t (LTT), ph agocytos is te s t (oxidative burs t, im puls e cytoph otom e tric inge s tion te s t), te s ts conce rning th e ability to activate lym ph ocyte s de pe nde nt on s ubcate gorie s (CD 69 - e xpre s s ion), te s ting of ability to induce CD 40L. Autologous antibody diagnos tics To diagnos e and to controlth e de ve lopm e nt of co lage nos is, vas culatitis and oth e r im m unode fie ncy- re late d dis e as e s w e ide ntify a large num be r of antibodie s w ith th e h e lp of im m unofluore s ce nce or ligand as s ays. Applie d m e th ods are continuous ly e valuate d and diagnos tic te ch niq ue s are im prove d (for e xam ple introduction of CCP antibody de te rm ination in cas e of rh e um atoid arth ritis ). A le rgy diagnos tics Type I- and type III- a le rgy diagnos e s are pe rform e d by q uantifying s pe cific IgE and IgG w ith th e h e lp of ligand as s ays. H LA typing H LA typing is us e d to ide ntify antige ns re s pons ible for h is tocom patibility. According to th e purpos e of th e typing th e proce dure can vary in its com ple xity. To s e le ct fitting donors and re ce ive rs for organ trans plantations s e rologicalh LA typing for clas s I and clas s II (m icrolym ph ocytotox te s t) and m ole cular ge ne tic typing for clas s I and clas s II are pe rform e d. H LA typing is als o us e d to diagnos e ce rtain dis e as e s or to clas s ify blood donors w h o s h a lbe us e d as th rom bocyte donors. UNIVERSITY CLINIC GREIFSW ALD QUALITY REPORT FOR PATIENTS 2006
4 Th e m ajor h is tocom patibility com ple x in th e h um an body is ca le d h um an le uk ocyte antige ne s s ys te m (H LA). M ultitrans funde d patie nts w h o are s uppos e d to be im m une agains t h is to- antibodie s of blood donors are als o te s te d for H LA antige ns. To provide th e s e patie nts w ith blood products th e various H LA antige ns are ide ntifie d and lis ts of oth e r s uitable donors are com pos e d QUALITY REPORT FOR PATIENTS 2006 UNIVERSITY CLINIC GREIFSW ALD
5 SPECIAL AREAS OF M ED ICAL CARE Gre ifs w ald data bas e of bone m arrow donors Th e ins titute h as a data bas e containing pote ntialvoluntary bone m arrow donors. Th e data bas e is re gularly e xte nde d and via th e Ge rm an Re gis te r of Bone M arrow D onors (D e uts ch e s Knoch e nm ark s pe nde r- Re gis te r) in Ulm th e data can als o be us e d by oth e r ins titutions a love r th e w orld. Ove r 5,000 pote ntialdonors are re gis te re d in th e Gre ifs w ald data bas e. Th e data bas e is one out of 35 Ge rm an data bas e s w h ich re gularly pe rform s m ole cular biologicaltypings and w h ich s e nds blood s am ple s of our donors to laboratorie s of oth e r s e arch ce ntre s for ch e ck ing if donors fit to patie nts D e taile d inform ation for volunte e rs for bone m arrow donations can be found unde r: h ttp://w w w.m e dizin.unigre ifs w ald.de /im m un/k noch e n m ark.h tm l QUALITY M ANAGEM ENT > Participation in inte rnaland e xte rnalinte rlaboratory te s ts (e.g. INSTANT ce rtification) > Fas t e valuation of q uality m anage m e nt m e as ure s. Ne ce s s ary ch ange s are dis cus s e d and th e ir re alization is contro le d UNIVERSITY CLINIC GREIFSW ALD QUALITY REPORT FOR PATIENTS 2006
6 INSTITUT FÜR IM M UNOLOGIE UND TRANSFUSIONSM ED IZ IN ABTEILUNG IM M UNOLOGIE RESEARCH Th e ins titute doe s re s e arch in th e fo low ing fie lds : > Infe ction im m unology (s e ps is, s e ptic s h ock s ) and > Ps ych ologicalne uroim m unology (h ow doe s s tre s s influe nce th e im m une de fe nce ). In our re s e arch data bas e you can alw ays find curre nt re s e arch re ports of our clinic: h ttp://fodb.uni- gre ifs w ald.de /inde x.ph p. QUALITY REPORT FOR PATIENTS 2006 UNIVERSITY CLINIC GREIFSW ALD
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