CURRICULUM VITAE. NAME: Guillermo A. Altenberg DATE: April Chair, Department of Cell Physiology and Molecular Biophysics,

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1 CURRICULUM VITAE NAME: Guillermo A. Altenberg DATE: April 2016 PRESENT POSITION AND ADDRESS EDUCATION Professor and Chair Department of Cell Physiology and Molecular Biophysics Director of the Center for Membrane Protein Research Texas Tech University Health Sciences Center School of Medicine Lubbock, Texas Telephone: (806) FAX: (806) Biology B.A. National School, San Martin, Buenos Aires, Argentina 1983 Medicine M.D. University of Buenos Aires, Buenos Aires, Argentina 1987 Doctor in Medicine Ph.D. University of Buenos Aires, and Physiology Buenos Aires, Argentina PROFESSIONAL AND TEACHING EXPERIENCE 2014-present Chair, Department of Cell Physiology and Molecular Biophysics, Texas Tech University Health Sciences Center (TTUHSC) Director, Center for Membrane Protein Research, School of Medicine, TTUHSC present 2014-present 2011-present 2011-present Professor, Joint Appointment, Department of Medical Education, TTUHSC. Affiliate Member of the Biotechnology Area of Cncentration, Graduate School of Biomedical Sciences, TTUHSC. Professor, Department of Cell Physiology and Molecular Biophysics, TTUHSC. Member, TTUHSC Cancer Center.

2 Vice-Chairman, Department of Cell Physiology and Molecular Biophysics, TTUHSC Director, Membrane Protein Core Laboratory, Center for Membrane Protein Research, TTUHSC present Member, Center for Membrane Protein Research, TTUHSC Associate Professor, Department of Cell Physiology and Molecular Biophysics, TTUHSC present Full Member of the Molecular Biophysics Area of Cncentration, Graduate School of Biomedical Sciences, TTUHSC Assistant Professor, Department of Internal Medicine, The University of Texas Medical Branch, Galveston, Texas Senior Scientist, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, Texas Member of the Graduate School of Biomedical Sciences, The University of Texas Medical Branch, Galveston, Texas Assistant Professor, Department of Physiology and Biophysics (name changed to Neuroscience and Cell Biology in 2004), The University of Texas Medical Branch, Galveston, Texas Associate Director, Membrane Protein Laboratory, Department of Physiology and Biophysics, The University of Texas Medical Branch, Galveston, Texas Research Assistant Professor, Department of Physiology and Biophysics, The University of Texas Medical Branch, Galveston, Texas Instructor, Department of Physiology and Biophysics, The University of Texas Medical Branch, Galveston, Texas Postdoctoral Fellow, Department of Physiology and Biophysics, The University of Texas Medical Branch, Galveston, Texas Chief Instructor, Department of Physiology, University of Buenos Aires, Argentina.

3 Instructor, Department of Physiology, University of Buenos Aires, Argentina Fellow of the National Research Council of Argentina (CONICET), Institute of Cardiological Research, University of Buenos Aires, Argentina Teaching Assistant, Department of Physiology, University of Buenos Aires, Argentina. RESEARCH ACTIVITIES Research Training Renal pathophysiology and hypertension, Institute of Cardiological Research, Hypertension Section, under the direction of Dr. Alberto C. Taquini, University of Buenos Aires, Argentina Renal pathophysiology and epithelial transport, Institute of Cardiological Research, Renal Section, under the direction of Dr. Carlos E. Amorena, University of Buenos Aires, Argentina Epithelial transport, epithelial electrophysiology, and fluorescence techniques applied to epithelia, under the direction of Dr. Luis Reuss, The University of Texas Medical Branch Molecular biology, in the laboratory of Dr. J-T. Zhang, The University of Texas Medical Branch Crystallization of membrane proteins in lipidic cubic phases, with Dr. Ehud M. Landau, Membrane Protein Laboratory, The University of Texas Medical Branch. Area of Research Structure/function relationships of membrane transport proteins. My major interests are connexins, the membrane proteins that form the gap junctions, and multidrug-resistance proteins of the ATP-binding cassette superfamily. Research support Current American Heart Association, Texas Affiliate Inc. Grant-in-Aid award 14GRNT , "Regulation of Cx43 by phosphorylation" (1/1/ /31/2016; role in project: Principal Investigator). Center for Membrane Protein Research Collaborative Grant Program, TTUHSC, Environment of the first transmembrane helix of connexin 26 in

4 hemichannels studied by electron paramagnetic resonance spectroscopy (PIs: Guillermo Altenberg/Luis Cuello, 5/1/2011-4/30/2016). Center for Membrane Protein Research Collaborative Grant Program, TTUHSC, Development of a high-throughput screening assay for connexins (PIs: Guillermo Altenberg/Luis Cuello, 9/1/2012-8/31/2016). Past RP Cancer Prevention & Research Institute of Texas, Individual Investigator Award, Molecular mechanisms of novel inhibitors of the multidrug resistance P-glycoprotein (PI: Guillermo Altenberg, 3/31/2010-3/31/2013). R01 GM National Institutes of Health. National Institute of General Medical Sciences, "Architecture of the transmembrane pore formed by connexin 43" (PI: Guillermo Altenberg, 8/1/2007-5/31/2013). 3R01 GM S1. National Institutes of Health. National Institute of General Medical Sciences, Administrative supplement to accelerate the pace of progress of R01 GM79629 (PI: Guillermo Altenberg, 9/30/2009-8/31/2011). Texas Advanced Research Program , Texas Higher Education Coordinating Board, Molecular mechanisms of regulation by calcium of gap-junctional hemichannels formed by connexin 43 (PI: Guillermo Altenberg, 5/15/2008-1/31/2011). R01 GM068586, National Institutes of Health. National Institute of General Medical Sciences, "Mechanisms of regulation of connexin 43 (PI: Luis Reuss, Co-I: Guillermo Altenberg, 8/1/2004-7/31/2009). National Institutes of Health. National Institute of Deafness and Communication Disorders, R21 DC "Mutant cochlear connexins associated with deafness" (9/1/2005-8/31/2008); role in project: Principal Investigator. American Heart Association, Texas Affiliate Inc., Grant-in-Aid Y, "Identification of the pore-lining helices of connexin 43 gap-junctional hemichannels (7/1/2007-8/31/2009; role in project: Principal Investigator). Relinquished on 7/31/2007 because of funding of the overlapping award R01 GM John Sealy Memorial Endowment Fund for Biomedical Research. "Mutant cochlear connexins associated with deafness" (6/1/2005-8/31/2005); role in project: Principal Investigator.

5 National Institutes of Health. National Institute of Diabetes and Digestive and Kidney Diseases, R21 DK "Development of genetically-encoded glucose sensors" (1/ /2004; role in project: Principal Investigator). National Institutes of Health. National Cancer Institute grant R29 CA "Phosphorylation and drug transport by P-glycoprotein" (7/1997-6/2002; role in project: Principal Investigator). Collaborative Grant from the Sealy Center for Structural Biology. "Structure of a carrier protein from E. coli" (4/1997-3/1998; role in project: Principal Investigator). American Heart Association, Texas Affiliate Inc. Grant-in-Aid award. "Regulation of drug transport by P-glycoprotein via protein kinase C- mediated phosphorylation" (7/1996-6/1997; role in project: Principal Investigator). UTMB Small Grant. "Identification of an extracellular domain of P- glycoprotein that regulates transport of chemotherapeutic agents" (5/1996-4/1997; role in project: Principal Investigator). Searle Research and Development. "A novel blocker of P-Glycoproteinmediated transport" ( ; role in project: Co-Investigator). John Sealy Memorial Endowment Fund. "Ion-channel function of P- glycoprotein and its role in multidrug resistance (8/1992-7/1994; role in project: Co-Investigator). UTMB Small Grant. "Mechanisms of Reduced Drug Uptake by Multidrug Resistant Cells" (4/1991-3/1992; role in project: Principal Investigator). National Research Council of Argentina. External postdoctoral fellowship ( ). National Research Council of Argentina. Fellowship ( ). COMMITTEE RESPONSIBILITIES Renal Physiology Committee of the Argentine Society of Nephrology ( ). Mason Guest Distinguished Lectureship Committee, Department of Physiology and Biophysics, The University of Texas Medical Branch ( ). Confocal Facility co-director, Department of Physiology and Biophysics, The University of Texas Medical Branch ( ).

6 Graduate School of Biomedical Sciences, Cellular Physiology and Molecular Biophysics Curriculum Committee, The University of Texas Medical Branch ( ). Renal/Fluid/Electrolytes Course Curriculum Committee Member, School of Medicine, The University of Texas Medical Branch ( ). Basic Biomedical Science Curriculum Committee, Graduate School of Biomedical Sciences, The University of Texas Medical Branch ( ). Graduate School of Biomedical Sciences Curriculum Committee, The University of Texas Medical Branch ( ). Gap-junction Session, co-chair, Biophysical Society Meeting (2005). Structural Biology Symposia, Sealy Center for Structural Biology and Molecular Biophysics, Organizing Committee ( ). Seminars Program, chair, Department of Cell Physiology and Molecular Biophysics, TTUHSC ( ). Space Committee, Department of Cell Physiology and Molecular Biophysics, TTUHSC ( ). Common Equipment Chair, Department of Cell Physiology and Molecular Biophysics, TTUHSC ( ). Conflict of Interest Committee, member, TTUHSC ( ). Bridge Support Review Committee, Bridge Funding Program for the TTUHSC School of Medicine ( ). TTUHSC Core Facilities Planning and Oversight Committee, member ( ). Preliminary Data Grant Program, TTUHSC, Committee member ( ). Center for Membrane Protein Research, Steering Committee, TTUHSC School of Medicine, member ( ). Imaging Core Design Committee, TTUHSC, member ( ). TTUHSC Cancer Committee, member (2009-present). TTUHSC School of Medicine Faculty Development Leave Committee, member

7 ( ). TTUHSC School of Medicine Tenure and Promotion Committee, member ( ). TTUHSC Graduate School of Biomedical Sciences, Dean Search Committee member (2012). TTUHSC School of Medicine, Pharmacology and Neuroscience Chairman Search Committee member (2013). Bioimaging Core Advisory Committee. TTUHSC School of Medicine ( ). TTU School of Arts and Sciences, Chemistry and Biochemistry Structural Biology Search Committee member ( ). Research Council Committee (advisory to the Senior Vice President for Research), TTUHSC (since 2014). Core Laboratory Advisory Committee. (advisory to the Senior Vice President for Research), TTUHSC (since 2014). Faculty Retention Subcommittee (advisory to the Senior Vice President for Research), TTUHSC, member (2014). Recruitment Strategy Subcommittee (advisory to the Senior Vice President for Research), TTUHSC, chair (2014). Faculty Retention Strategic Planning Implementation Team, Convener, Office of Research, TTUHSC (2015-present). Recruitment Strategic Planning Implementation Team, Member, Office of Research, TTUHSC (2015-present). TEACHING RESPONSIBILITIES School of Medicine Lecturer, Renal Physiology and Electrolytes course, University of Buenos Aires ( ). Facilitator, Renal Physiology and Electrolytes course, University of Buenos Aires ( ). Facilitator Assistant, Renal Physiology and Electrolytes course, University of Buenos Aires ( ). Lecturer, Human Physiology, Renal Physiology and Electrolytes course, The University of Texas Medical Branch ( ). Small group problem discussions, Human Physiology, Renal Physiology and Electrolytes course, The University of Texas Medical Branch ( ).

8 Lecturer, Renal/Fluids/Electrolytes, The University of Texas Medical Branch ( ). Facilitator, Renal/Fluids/Electrolytes, The University of Texas Medical Branch ( ). Facilitator, Great Syndromes, The University of Texas Medical Branch ( ). Structure and Function of the Organ Systems, TTUHSC (2007-present). Renal Section Leader, Structure and Function of the Organ Systems, TTUHSC (2009-present). Graduate School Laboratory techniques. Cell Biology Program. Lectures and discussions on fluorescent techniques. The University of Texas Medical Branch ( ). Electrophysiological techniques. Cellular Physiology and Molecular Biophysics Program. Lectures and discussion on epithelial electrophysiology. The University of Texas Medical Branch ( ). Membrane protein structure and function. Cellular Physiology and Molecular Biophysics Program. Lectures and discussion on structure-function relationship of ATP-binding cassette proteins. The University of Texas Medical Branch ( ). Ion channel structure and function. Cellular Physiology and Molecular Biophysics Program. Lectures and discussion on ion channel structure. The University of Texas Medical Branch ( ). Laboratory Methods in Physiology. Cell Physiology and Molecular Biophysics Program. Lectures and laboratory training on electrophysiology of ion channels. TTUHSC (2007-present). Physiology Special Topics: Membrane Proteins. Cell Physiology and Molecular Biophysics Program. Discussions on membrane protein structure and function. TTUHSC (2007-present). Human Physiology. Renal Section Leader, organization, lectures and group discussions. TTUHSC (2007-present). Topics in Biochemistry and Laboratory Methods. Biochemistry and Molecular Genetics Program. Course Director and teacher. TTUHSC (2008). Advanced Topics in Physiology. Cell Physiology and Molecular Biophysics Program. Course Director and teacher. TTUHSC ( ). Molecular Cell Physiology. Cell Physiology and Molecular Biophysics Program. Course Director and teacher. TTUHSC (2014). Students/Mentees/Advisees/Trainees Post-doctoral fellows Ariel Castro, Ph.D. Currently an Associate Professor, Department of Biochemistry and Molecular Biology, University of Concepcion, Chile. Xiaoyong Bao, Ph.D. Currently an Associate Professor in the Pediatrics Child Health Research Center, The University of Texas Medical Branch at Galveston. Mauricio Retamal, Ph.D. Currently an Assistant Professor at University for Development, Santiago, Chile.

9 Sung Chang Lee, Ph.D. Currently a Research associate in the Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA. Maria Elena Zoghbi, Ph.D. Current postdoctoral fellow. Mariana Fiori, Ph.D. Current postdoctoral fellow. Leo Mok, Ph.D. Currently a Scientist at Amgen Inc., Thousand Oaks, California. Masters, Ph.D. and M.D./Ph.D. students Alejandro Sarries, M.D. Currently a Cardiologist in Chubut, Argentina. Brian Button, Ph.D. Currently an Assistant Professor, Pulmonary Diseases and Critical Care Medicine, Department of Medicine, University of North Carolina, School of Medicine at Chapel Hill. Ernest S. Han, M.D., Ph.D. Currently an Assistant Professor in the Department of Surgery, City of Hope, Duarte, CA. Denise M. Wilkes, M.D., Ph.D. Currently an Assistant Professor in the Department of Anesthesiology, The University of Texas Medical Branch at Galveston. Yongyue Chen, M.D., Ph.D. Currently an Assistant Professor in the Department of Psychiatry at Virginia Tech Carilion School of Medicine, Roanoke, VA. Sung Haeng Lee, Ph.D. Currently an Assistant Professor at Chosun University College of Medicine, South Korea. Sung Chang Lee, Ph.D. Currently a Research associate at The Scripps Institute, La Jolla, California. Neha Dole, M.S. Currently a graduate student at the University of Connecticut. Srinivasan Krishnan, M.S. Currently a graduate student at TTUHSC. Abdullatif Salah Kassar, M.S. Currently a graduate student at Texas A & M University. Rebecca Sisson Cooper, Ph.D. Currently a postdoctoral fellow at Tufts University in Boston, Massachusetts. Ramesh Kumar, M.S. Completed his internship of the Texas Tech University Biotechnology Program in Greg Fendley, M.S. Currently a graduate student at TTUHSC. Chair/Member of Ph.D. Supervisory Committee Brian Button. Ph. D. student, The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Denise Wilkes, M.D., Ph.D. student. The University of Texas Medical Branch Cell Physiology and Molecular Biophysics Program. Committee Chair, completed in Ernest S. Han, M.D., Ph.D. student. The University of Texas Medical Branch Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Xiaoyong Bao. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Yongyue Chen. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Sung Haeng Lee. The University of Texas Medical Branch, Cell Physiology and

10 Molecular Biophysics Program. Committee Chair, completed in Naomi Oshiro. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Zhifang Zhao. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Completed in Sung Chang Lee. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Chair, completed in Liang Ma. External Committee Member. The University of Texas Medical Branch, Cell Physiology and Molecular Biophysics Program. Committee Member, completed in Shouryadeep Srivastava. TTUHSC, Pharmacology Program. Committee Member, completed in Rebecca Sisson Cooper. TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Chair, completed in Ashujit Tagle. TTUHSC, Pharmacology Program. Committee Member, completed in Srinivasan Krishnan, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Chair, in progress. Greg Fendley, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Chair, in progress. Swapneeta Date, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Member, in progress. Ty Whisenant, M.D., Ph.D. student, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Member, in progress. Spandana Vemulapally, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Member, in progress. Kelsey Markham, TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Member, in progress. Chair/Member of Masters Supervisory Committee Greg Young, Honors in Research Committee Member, The University of Texas Medical Branch School of Medicine. Committee Member, completed in Andrei Bednov. The University of Texas Medical Branch, Bioengineering Program. Committee Member, completed in Neha Dole. Texas Tech University, Biotechnology Program. Research Internship Advisor and Committee Member, completed in Srinivasan Krishnan, Texas Tech University Biotechnology Program. Research Internship Advisor and Committee Member, completed in Abdullatif Salah Kassar. TTUHSC, Cell Physiology and Molecular Biophysics Program. Committee Chair, completed in Ramesh Kumar, Texas Tech University Biotechnology Program. Research Internship Advisor and Committee Member, completed in May Greg Fendley, Texas TTUHSC, Biotechnology Program. Research Internship Advisor and Committee Chair, completed in MEMBERSHIP IN ELECTED SCIENTIFIC SOCIETIES

11 American Association for the Advancement of Science. American Association for Biochemistry and Molecular Biology. American Heart Association American Society for Cell Biology. Argentine Society of Neurosciences (Honorary member). Biophysical Society. Society of General Physiologists. HONORS Honor Diploma, Medical School, University of Buenos Aires (1983). Honorary member of the Argentine Society of Neurosciences (since 2008). Unsung Hero Award, Department of Cell Physiology and Molecular Biophysics, TTUHSC (2008). Dean s Research Award, Texas Tech University School of Medicine (2009). Chancellor's Council Distinguished Research Award (2013) Competitive Fellowships: Fellowship of the National Research Council of Argentina ( ). External postdoctoral fellowship of the National Research Council of Argentina ( ). JOURNALS AND GRANTSREVIEWER Journal editorial member for: International Journal of Biochemistry and Molecular Biology Frontiers in Physiology. Membrane Physiology and Membrane Biophysics Journal reviewer for: Acta Physiologica et Pharmacologica Latinoamericana. Aging Cell. American Journal of Physiology. Biochimica and Biophysica Acta. Biology of the Cell Biophysical Journal. Cancer Research. Chemico-Biological Interactions. Current Molecular Medicine. European Journal of Biochemistry. European Journal of Neuroscience. Pflügers Archives. European Journal of Physiology. FEBS letters.

12 International Journal of Biochemistry and Cell Biology International Journal of Biochemistry and Molecular Biology International Journal of Cancer. Journal of Biological Chemistry. Journal of General Physiology. Journal of Membrane Biology. Leukemia Research. Medicina (Bs.Aires). Pflügers Archives-European Journal of Physiology Structure. The Journal of Physiology (London). Grant reviewer for: American Cancer Society Institutional Research Grant Program. Intramural Research Grants and Fellowships ( ). American Cancer Society Institutional Research Grant Program Committee (1999). Cancer Research Campaign Grants, United Kingdom ( ). John Sealy Memorial Endowment Fund for Biomedical Research (1999). Pilot Project Applications. Centennial Center for Environmental Toxicology (1998). National Science Foundation (2002). James W. McLaughlin Fellowship Fund Committee (2003). Medical Research Council, Great Britain (2004-present). Health Research Council of New Zealand (2006) Agencia Nacional de Promoción Científica y Tecnológica, Fondo para la Investigación Científica y Tecnológica (FONCyT), Argentina (2007-present). German-Israeli Foundation (2007-present). American Heart Association, Region III Review Consortium, Committee 5A (since ). Bridge Funding Program for the TTUHSC School of Medicine (since 2008). National Institutes of Health, National Institute of General Medical Sciences, Challenge Grant reviewer, American Heart Association, National, Cardiorenal Unified Peer Review Committee ( ). American Heart Association, Basic Cell Protein Crystallography 1 Peer Review Committee (since 2013). National Institutes of Health ( ). PUBLICATIONS Articles in peer-reviewed journals Altenberg GA, Negri AL, Rainoldi FA, Prigollini D, Yeyati NL. Acute effect of amiloride on urinary magnesium excretion in dogs. Acta Physiol Pharmacol Latinoam 36:89-92, Yeyati NL, Altenberg GA, Rainoldi FA, Greco J. Reversal by indomethacin of the renal effects of dopamine in subjects with normal renal function. Acta Physiol Pharmacol Latinoam 36: , 1986.

13 Altenberg GA, Aristimuno PC, Amorena CE, Taquini AC. Amiloride prevents the metabolic acidosis of a KCl load in nephrectomized rats. Clin Sci (Lond) 76: , Altenberg GA, Reuss L. Apical membrane Na + /H + exchange in Necturus gallbladder epithelium. Its dependence on extracellular and intracellular ph and on external Na + concentration. J Gen Physiol 95: , Stoddard JS, Altenberg GA, Ferguson ML, Reuss L. Furosemide blocks basolateral membrane Cl - permeability in gallbladder epithelium. Am J Physiol Cell Physiol 258:C1150-C1158, Altenberg GA. Intracellular alkalosis induced by increasing extracellular potassium. Ionic dependence and effects of amiloride and DIDS. Miner Electrolyte Metab 16: , Mac Laughlin M, Altenberg G, Gioioso E, Amorena CE, Taquini AC. Natriuretic response to saline load in one-kidney/one-clip hypertensive rats. Renal Physiol Biochem 13: , Altenberg GA, Stoddard JS, Reuss L. Electrophysiological effects of basolateral [Na + ] in Necturus gallbladder epithelium. J Gen Physiol 99: , Yeyati NL, Altenberg GA, Adrogue H. Mechanism of the acetazolamide-induced rise in renal resistance assessed in the dog whole kidney. Renal Physiol Biochem 15:99-105, Altenberg GA, Young G, Horton JK, Glass D, Belli JA, Reuss L. Changes in intra- or extracellular ph do not mediate P-glycoprotein-dependent multidrug resistance. Proc Natl Acad Sci USA 90: , Altenberg GA, Subramanyam M, Bergmann JS, Johnson KM, Reuss L. Muscarinic stimulation of gallbladder epithelium. I. Electrophysiology and signalling mechanisms. Am J Physiol Cell Physiol 265:C1604-C1612, Altenberg GA, Subramanyam M, Reuss L. Muscarinic stimulation of gallbladder epithelium. II. Fluid transport, cell volume, and ion permeabilities. Am J Physiol Cell Physiol 265:C1613-C1619, Altenberg GA, Deitmer JW, Glass DC, Reuss L. P-glycoprotein-associated Cl - currents are activated by cell swelling but do not contribute to cell volume regulation. Cancer Res 54: , Altenberg GA, Vanoye CG, Han ES, Deitmer JW, Reuss L. Relationship between P- glycoprotein-mediated rhodamine 123 transport, cell volume, and Cl - currents. J Biol Chem 269: , 1994.

14 Altenberg GA, Vanoye CG, Horton JK, Reuss L. Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane. Proc Natl Acad Sci USA 91: , Sognier MA, Zhang Y, Eberle RL, Sweet KM, Altenberg GA, Belli JA. Sequestration of doxorubicin in vesicles in a multidrug-resistant cell line (LZ-100). Biochem Pharmacol 48: , Altenberg GA, Subramanyam M, Reuss L. Muscarinic stimulation of gallbladder epithelium. III. Antagonism of adenosine 3', 5'-cyclic monophosphate-mediated effects. Am J Physiol Cell Physiol 267:C1196-C1202, Subramanyam M, Altenberg GA, Reuss L. Dopamine activates apical membrane Cl - conductance and inhibits fluid absorption in amphibian gallbladder by elevating intracellular camp. Cell Physiol Biochem 5: , Han ES, Vanoye CG, Altenberg GA, Reuss L. P-glycoprotein-associated chloride currents revealed by specific block by an anti-p-glycoprotein antibody. Am J Physiol Cell Physiol 270:C1370-C1378, Torres RJ, Altenberg GA, Copello JA, Zampighi G, Reuss L. Preservation of structural and functional polarity in isolated epithelial cells. Am J Physiol Cell Physiol 270:C1864- C1874, Torres RJ, Altenberg GA, Cohn JA, Reuss L. Polarized expression of cyclic AMPactivated chloride channels in isolated epithelial cells. Am J Physiol Cell Physiol 271:C1574-C1582, Torres RJ, Subramanyam M, Altenberg GA, Reuss L. Cell swelling activates K + and inhibits Cl - conductance of the basolateral membrane of cells from a leaky epithelium. J Gen Physiol 109:1-12, Castro AF, Altenberg GA. Inhibition of drug transport by genistein in multidrug-resistant cells expressing P-glycoprotein. Biochem Pharmacol 53:89-93, Vanoye CG, Altenberg GA, Reuss L. P-glycoprotein is not a swelling-activated Cl - channel; possible role as a Cl - channel regulator. J Physiol (Lond) 502: , Lee JS, Scala S, Matsumoto Y, Dickstein B, Robey R, Zhang Z, Altenberg G, Bates SE. Reduced drug accumulation and multidrug resistance in human breast cancer cells without associated P-glycoprotein or MRP overexpression. J Cell Biochem 65: , Horton JK, Thimmaiah KN, Altenberg GA, Castro AF, Germain GS, Gowda GK, Houghton PJ. Characterization of a novel bis-acridone and comparison with PSC-833 as a potent and poorly reversible modulator of P-glycoprotein. Mol Pharmacol 52: ,

15 1997. Wang C, Castro AF, Wilkes DM, Altenberg GA. Expression and purification of the first nucleotide-binding domain and linker region of MDR1. Comparison of fusions to glutathione-s-transferase, thioredoxin and maltose-binding protein. Biochem J 338:77-81, Vanoye CG, Castro AF, Pourcher T, Reuss L, Altenberg GA. Phosphorylation of P- glycoprotein by PKA and PKC modulates swelling-activated Cl - currents. Am J Physiol Cell Physiol 276:C370-C378, Vanoye CG, Altenberg GA, Reuss L. Inhibition of P-glycoprotein-mediated transport by a hydrophobic contaminant in commercial gluconate salts. Am J Physiol Cell Physiol 276:C1439-C1442, Castro AF, Horton JK, Vanoye CG, Altenberg GA. Mechanism of inhibition of P- glycoprotein-mediated drug transport by protein kinase C blockers. Biochem Pharmacol 58: , Reuss L, Vanoye CA, Altenberg GA, Vergara L, Subramanyam M, Torres R. Cellvolume and ion conductances in amphibian gallbladder epithelium. Cell Physiol Biochem 10: , Button B, Reuss L, Altenberg GA. PKC-mediated stimulation of amphibian CFTR depends on a single phosphorylation consensus site. Insertion of this site confers PKC sensitivity to human CFTR. J Gen Physiol 117: , Wilkes DM, Wang C, Aristimuño P, Castro AF, Altenberg GA. Nucleotide triphosphatase activity of the N-terminal nucleotide-binding domains of the multidrug resistance proteins P-glycoprotein and MRP1. Biochem Biophys Res Commun 296: , Lee SH, Altenberg GA. Transport of leukotriene C 4 by a cysteine-less multidrug resistance protein 1 (MRP1). Biochem J 370: , Lee SH, Altenberg GA. Expression of functional multidrug-resistance protein 1 (MRP1) in Saccharomyces cerevisiae. Effects of N- and C-terminal affinity tags. Biochem Biophys Res Commun 306: , Bao X, Altenberg GA, Reuss L. Mechanism of regulation of the gap-junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C , Bao X, Cheng Y, Reuss L, Altenberg GA. Functional expression in Xenopus oocytes of gap-junctional hemichannels formed by a cysteine-less connexin 43. J Biol Chem 279: , 2004.

16 Bao X, Reuss L, Altenberg GA. Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of serine 368. J Biol Chem 279: , Chen Y, Altenberg GA, Reuss L. Mechanism of the activation of Xenopus CFTR by stimulation of PKC. Am J Physiol Cell Physiol 287:C1256-C1263, Chen Y, Button B, Altenberg GA, Reuss L. Potentiation of the effect of PKA stimulation of Xenopus CFTR by activation of PKC. Role of NBD2. Am J Physiol Cell Physiol 287:C , Bao X, Chen Y, Lee SH, Lee SC, Reuss L, Altenberg GA. Membrane transport proteins with complete replacement of transmembrane helices with poly-alanine sequences remain functional. J Biol Chem 280: , Chen Y, Deng Y, Bao X, Reuss L, Altenberg GA. Mechanism of the defect in gapjunctional communication by expression of a mutant connexin 26 associated with dominant deafness. FASEB J 19: , Deng Y, Chen Y, Reuss L, Altenberg GA. Mutations of connexin 26 at position 75 and dominant deafness: Essential role of arginine for the generation of functional gapjunctional channels. Hearing Res 220:87-94, Bao X, Lee SC, Reuss L, Altenberg GA. Change in permeant size-selectivity by phosphorylation of connexin 43 gap-junctional hemichannels by PKC. Proc Natl Acad Sci USA 104: , Kelly B, Lozano A, Altenberg G, Makishima T. Connexin 26 mutation in Keratitis- Ichthyosis-Deafness (KID) syndrome in mother and daughter with combined conductive and sensorineural hearing loss. Int J Dermatol 47: , Retamal MA, Yin SY, Altenberg GA, Reuss L. Modulation of Cx46 hemichannels by nitric oxide. Am J Physiol Cell Physiol 296:C1356-C1363, Retamal MA, Yin SY, Altenberg GA, Reuss L. Voltage-dependent facilitation of Cx46 hemichannels. Am J Physiol Cell Physiol 298:C132-C139, Schalper KA, Sánchez HA, Lee SC, Altenberg GA, Nathanson MH, Sáez JC. Connexin 43 hemichannels mediate the Ca 2+ influx induced by extracellular alkalinization. Am. J. Physiol. Cell Physiol 299:C , Retamal MA, Evangelista-Martínez F, León-Paravic CG, Altenberg GA, Reuss L. Biphasic effect of linoleic acid on connexin 46 hemichannels. Pflugers Arch 461: , 2011.

17 Young G, Reuss L, Altenberg GA. Altered intracellular ph regulation in cells with high levels of P-glycoprotein expression. Int J Biochem Mol Biol 2: , Niwayama S, Kassar AS, Zhao T, Sutton RB, Altenberg GA. A pyrene maleimide with a flexible linker for sampling of longer inter-thiol distances by excimer formation. PLoS ONE 6(10):e26691, Zoghbi ME, Fuson KL, Sutton RB, Altenberg GA. Kinetics of the association/ dissociation cycle of an ATP-binding cassette nucleotide-binding domain. J Biol Chem 287: , Zoghbi ME, Krishnan S, Altenberg GA. Dissociation of ATP-binding cassette nucleotidebinding domain dimers into monomers during the hydrolysis cycle. J Biol Chem 287: , Fiori MC, Figueroa V, Zoghbi ME, Saéz JC, Reuss L, Altenberg GA. Permeation of calcium through purified connexin 26 hemichannels. J Biol Chem 287: , Cooper RS, Altenberg GA. Association/dissociation of the nucleotide-binding domains of the ATP-binding cassette protein MsbA measured during continuous hydrolysis. J Biol Chem 288: , Zoghbi ME, Altenberg GA. Hydrolysis at one of the two nucleotide-binding sites drives the dissociation of ATP-binding cassette nucleotide-binding domain dimers. J Biol Chem 288: , Zoghbi ME, Altenberg GA. ATP binding to two sites is necessary for dimerization of nucleotide-binding domains of ABC proteins. Biochem Biophys Res Commun 443:97-102, Swartz DJ, Mok L, Botta SK, Singh A, Altenberg GA, Urbatsch IL. Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance. Biosci Rep 34(3):e00116, León-Paravic CG, Figueroa V, Guzmán DJ, Valderrama CF, Vallejos AA, Fiori MC, Altenberg GA, Reuss L, Retamal MA. Carbon monoxide (CO) is a novel inhibitor of connexin hemichannels. J Biol Chem 289: , Fiori MC, Krishnan S, Cortés DM, Retamal MA, Reuss L, Altenberg GA, Cuello LG. Functional hemichannels formed by human connexin 26 expressed in bacteria. Biosci Rep Biosci Rep. 35(2): e00177, Zoghbi ME, Cooper RS, Altenberg GA. The lipid bilayer modulates the structure and function of an ATP-binding cassette exporter. J Biol Chem 291: , 2016.

18 Dalamon V, Fiori MC, Figueroa VA, Oliva CA, Del Rio R, Gonzalez W, Canan JG, Elgoyen AB, Altenberg GA, Retamal MA. Gap-junctional channel and hemichannel activity of two recently-identified connexin 26 mutants associated with deafness. Pflugers Arch 468: , Alcaíno CA, González W, Canan JG, Moraga-Amaro R, Stehberg J, Del Rio R, Altenberg GA, Reuss L, Retamal MA. Cx46 hemichannel modulation by nitric oxide: Involvement of Cys218 and calcium permeability. Submitted. Figueroa VA, Oliva CA, Jara O, Martínez AD, Altenberg GA, Retamal MA. Linoleic acid induces lens epithelial cell death through the opening of connexin hemichannels. Submitted. Krishnan S, Cortes DM, Altenberg GA, Cuello LG. An E. coli-based assay to assess the function of recombinant human hemichannels. Submitted. Reviews and book chapters Reuss L, Segal Y, Altenberg G. Regulation of ion transport across gallbladder epithelium. Ann Rev Physiol 192: , Altenberg G, Copello J, Cotton C, Dawson K, Segal Y, Wehner F, Reuss L. Electrophysiological methods for studying ion and water transport in Necturus gallbladder epithelium. Methods Enzymol 53: , Reuss L, Altenberg GA. camp-activated Cl - channels: Regulatory role in gallbladder and other absorptive epithelia. News Physiol Sci 10:32-37, Altenberg GA, Reuss L. Measurements and interpretation of intracellular ionic activities. In: Epithelial transport: A guide to methods and experimental analysis. Chapter 7. NK Wills, L Reuss, SA Lewis, editors. Chapman and Hall, London, Reuss L, Cotton CU, Altenberg GA. Measurements of epithelial cell volume. In: Epithelial transport: A guide to methods and experimental analysis. Chapter 8. NK Wills, L Reuss, SA Lewis, editors. Chapman and Hall, London, Altenberg GA. The engine of ABC proteins. News Physiol Sci 18: , Altenberg GA, Chen Y, Button B, Reuss L. The role of protein kinase C in the regulation of the activity of the cystic fibrosis chloride channel. Nova Acta Leopoldina 89: , Altenberg GA. Structure of multidrug-resistance proteins of the ATP-binding cassette (ABC) superfamily. Curr Med Chem Anti-Canc Agents 4:53-62, Altenberg GA. The multidrug resistance protein P-glycoprotein and the regulation of

19 chloride channels. Leukemia Res 29: , Kovacs JA, Baker KA, Altenberg GA, Abagyan R, Yeager M. Molecular modeling and mutagenesis of gap junction channels. Prog Biophys Mol Biol 94:15-28, Altenberg GA, Bao X, Lee SC, Reuss L. Regulation of connexin 43 channels by PKCmediated phosphorylation, Physiological Mini-Reviews 3:35-40, Reuss L, Altenberg GA. Mechanisms of ion transport across cell membranes. In: The kidney. Chapter 2. DW Selding, G Giebisch, editors. Elsevier, San Diego, CA, Altenberg GA, Reuss L. Mechanisms of water transport across cell membranes and epithelia. In: The kidney. Chapter 4. DW Selding, G Giebisch, editors. Elsevier, San Diego, CA, Fiori MC, Reuss L, Cuello LG, Altenberg GA. Functional analysis and regulation of purified connexin hemichannels. Front Physiol 5:71, Kreir M, Altenberg GA. Functional assays of purified connexin hemichannels. In: Gap ganction channels and hemichannels. Chapter 10. D Bai, JC Saez, editors. CRC Press, Boca Raton, FL, In press.