Convenient Generation and Culture of Induced Pluripotent Stem Cells

Size: px
Start display at page:

Download "Convenient Generation and Culture of Induced Pluripotent Stem Cells"

Transcription

1 Pharma&Biotech BioResearch Convenient Generation and Culture of Induced Pluripotent Stem Cells Webinar 24 June 2014 / Speaker: Liz Horton 25 June 2014 / Speaker: Dr. Claudia Schwartz Lonza Walkersville, Inc., Walkersville, MD / June 2014 Lonza

2 Agenda Xeno-free, feeder-free culturing of hpscs L7 hpsc Culture System Generation of induced Pluripotent Stem Cells Reprogramming of PBMCs Adult stem cells An Overview 2

3 Agenda Xeno-free, feeder-free culturing of hpscs L7 hpsc Culture System Generation of induced Pluripotent Stem Cells Reprogramming of PBMCs Adult stem cells An Overview 3

4 Induced Pluripotent Stem Cells (ipscs) Basics Human blastocyst Somatic cells (e.g. fibroblasts or blood cells) Oct4, Klf4, Sox2, c-myc etc. Viral/non-viral transfection Inner Cell mass Culture and selection Human embryonic stem cell (hesc) Induced Pluripotent Stem Cell (ipsc) 4

5 Human PSC Culture What are the potential issues Every day feeding Feeder culture Cell recovery efficient attachment after passaging Differentiation bias Karyotype issues Viability issues Clinical aspirations 5

6 L7 hpsc Culture System: The Components Cell Culture Tools Colony Appearance Colony Expansion Banking L7 hpsc Medium BulletKit L7 hpsc Matrix L7 hpsc Passaging Solution L7 hpsc Cryosolution 6

7 Every-Other-Day Feeding Typical, Healthy Morphology Benefits Daily maintenance of hpscs no longer necessary Similar look and growth characteristics as every-day feeding 7

8 High Levels of Pluripotency Marker Expression Retained Maintained pluripotency Cells cultured for 40 passages in L7 hpsc Medium BulletKit show high level of pluripotency marker expression 8

9 L7 hpsc Culture System Maintains Normal Karyotype After 40 Passages 9

10 L7 hpsc Culture System Supports Embryoid Body Formation hesc-h9 EBs, day 1 EBs, day 7 Ectoderm Endoderm Mesoderm Tuj1 AFP Smal Human pluripotent stem cells cultured with L7 hpsc Culture System Maintain potency and can form embryoid bodies 10

11 And More Importantly, Directed Differentiation hipsc #6.4 Neurospheres Rosettes NSCs 11

12 Characteristic NSC Marker Expression Nestin Sox1 Nestin/DAPI Pax6 DAPI Nestin/Sox1 DAPI Nestin/Pax6 12

13 % Plating Efficiency % Plating Efficiency L7 hpsc Passaging Solution Provides High Re-Plating Efficiency 100% 90% 80% 70% 60% 50% StemPro Media 1 mtesr1 TM 1 Media 2 n=3 40% Dispase 30% 20% 10% 0% L7 hpsc Passaging Solution 13

14 The Time Required to Expand hpscs Using L7 hpsc Passaging Solution is Significantly Reduced L7 hpsc Passaging Solution Reagent L7 hpsc Passaging Solution Post- Dissociation Viability Days to Produce 2 x Cells 97% 27 Dispase 53% 55 Collagenase 51% 56 Scraping 35% 97 14

15 Summary: Advantages of L7 hpsc Culture System Every-other-day feeding High re-plating efficiency Maintainance of pluripotency marker expression Normal karyotype Unaltered directed differentiation capabilities 15

16 Agenda Xeno-free, feeder-free culturing of hpscs L7 hpsc Culture System Generation of induced Pluripotent Stem Cells Reprogramming of PBMCs Adult stem cells An Overview 16

17 Induced Pluripotent Stem Cells (ipscs) Basics Human blastocyst Somatic cells (e.g. fibroblasts or blood cells) Oct4, Klf4, Sox2, c-myc etc. Viral/non-viral transfection Inner Cell mass Culture and selection Human embryonic stem cell (hesc) Induced Pluripotent Stem Cell (ipsc) 17

18 Nucleofector Technology The Principle Notebook Control Unit High transfection efficiency combined with low mortality DNA is directed into the nucleus giving faster gene expression Nucleofection Process Other transfection methods 18

19 Components of Nucleofector Technology Notebook Control Unit A Unique Combination: Nucleofector Device Specific Nucleofector Kits Detailed optimized protocols Enabling excellent transfection performance combined with high functionality! 19

20 Generation of ips Cells via Transfection of PBMCs Using Nucleofector Technology Reviving & Priming Nucleofection Recovery Colony appearance Colony selection & expansion Banking & Characterization Day -9 Day 0 Day 0-4 Day 9 Day (P0) Day 76 (P5) L7 PBMC Reprogramming Protocol (coming soon) L7 hpsc Culture System 20

21 PBMC-Derived ipsc Characterization Attachment and Pluripotency Markers Morphology (P15, D3) Alkaline Phosphatase (P15, D4) DAPI TRA-1-60/Oct4 Lonza LiPSC-34B 21

22 PBMC-Derived ipsc Characterization Pluripotency Markers LiPSC34B (P13, D4) SSEA4-PE TRA-1-60-PE TRA-1-81-PE 22

23 PBMC-derived ipsc Characterization Embryoid Body Formation ipcs can be differentiated into cells representative of all 3 germ layers EB Colonies SMA, DAPI AFP, DAPI Tuj1, DAPI LiPSC34B P15 23

24 PBMC-Derived ipsc Characterization Karyotype and Plasmid Clearance Clear of Plasmid: LiPSC-34B (P5) Undetectable level of plasmid confirmed by a qpcr based assay based on the level of CT value Normal Karyotype: LiPSC-34B (P11) 24

25 ips Generation Typically Used Human Cells Types Human Cells* Efficiency (pmaxgfp Vector) References Dermal fibroblasts 40-95% Yu J et al. (2009) Science, 324(5928): Mehta A et al. (2011) Cardiovasc Res 91: Keratinocytes 40-80% Bone marrow CD34+ cells 66% Chou BK et al. (2011) Cell Research, 21: Cord blood CD34+ cells 82% Chou BK et al. (2011) Cell Research, 21: Mack A et al. (2011) PlosOne 6 (11) Bone marrow mononuclear cells Peripheral blood mononuclear cells Neural progenitor cells up to 90% Hu K et al. (2011) Blood, 117(14): e109-e119 Chou BK et al. (2011) Cell Research, 21: % Hu K et al. (2011) Blood, 117(14): e109-e119 Chou BK et al. (2011) Cell Research, 21: Adipose-derived stem cells 65-90% Jia et al. (2010) Nature Methods, 7: *Also available from Lonza 25

26 Agenda Xeno-free, feeder-free culturing of hpscs L7 hpsc Culture System Generation of induced Pluripotent Stem Cells Reprogramming of PBMCs Adult stem cells An Overview 26

27 Stem Cell Sources and Relevance ESCs Adult SCs IPSCs Origin Blastocyst of embryo Bone marrow, circulation or resident issue Strengths - Pluriotent (3 germ layers) - Self-renewal and high replicative capacity Weaknesses - Immunological concerns - Subject to ethical debate - Potential for teratoma and teratocarcinoma - Currently no clinical trial data - Autologous - Clinical safety and efficacy data - Typically lineage committed - Limited number - Limited replicative capacity - Lineage restricted Reprogramming of somatic cells - Totipotent (3 germ layers and trophoblast) - Autologous - Large reservoir of cells - Potential for teratoma and teratocarcinoma - No clinical data Leeper N J et al. Circulation. 2010;122:

28 Overview Adult Stem Cell and Precursors Real Adult Stem Cells Human ADSC Human MSC Rat MSC Precursors Human CD34+ cells Human pre-adipocytes Human osteoclast precursors Human neural progenitors Human motor neuron progenitors 28

29 Adult Stem Cells MSCs MSC studies: General Clinical trails Relevance: No ethical issues Immunoprivileged Tissue engineering 29

30 Efficient Transfection of Various Adult Stem Cell Types Using Nucleofector Technology 100% 80% 60% 40% 20% Efficiency Viability 0% For more data see: 30

31 Products and Services for Stem Cell Research Adult Stem Cells Precursors Pluripotent SC ipsc Cell Sourcing Human ADSC* Human MSC Rat MSC Human CD34+ cells Human pre-adipocytes Human osteoclast precursors Human neural progenitors Human motor neuron progenitors Human fibroblasts Human CD34+ cells Human MNCs MEFs Human keratinocytes Human ADSC Cell Culture L7 TM hpsc Culture System Transfection Nucleofector Technology Differentiation Differentiation media Services Characterization Services Expansion Services *normal and disease 31

32 Support Tools and Contact Details Our online databases Cell Database: Citations: Primary cell culture experts Scientific Support Team US: or Scientific Support Team EU + ROW: scientific.support.eu@lonza.com or

33 Interested in Learning More? Join our upcoming webinar in October: Gene Editing Transfecting ZFNs, TALENs or CRISPR/Cas Using Nucleofector Technology Register at: 33

34 Thank You!

GM23226*A. Certificate of Analysis

GM23226*A. Certificate of Analysis GM23226*A Certificate of Analysis Product Description Human fibroblast line reprogrammed with four factors (Oct 4, Sox 2, c-myc and Klf-4) using retroviral vector Publication(s) describing ipsc establishment

More information

Regulatory and Legal Frameworks for Offering Stem Cell Therapies in China. Qi ZHOU, Ph.D. Institute of Zoology Chinese Academy of Sciences

Regulatory and Legal Frameworks for Offering Stem Cell Therapies in China. Qi ZHOU, Ph.D. Institute of Zoology Chinese Academy of Sciences Regulatory and Legal Frameworks for Offering Stem Cell Therapies in China Qi ZHOU, Ph.D. Institute of Zoology Chinese Academy of Sciences Stem Cell Clinical Trials in The World Cited from World Stem Cell

More information

CHARACTERISTIC FEATURES OF STEM CELLS. CLONING TECHNOLOGIES

CHARACTERISTIC FEATURES OF STEM CELLS. CLONING TECHNOLOGIES CHARACTERISTIC FEATURES OF STEM CELLS. CLONING TECHNOLOGIES 1 Science is discovering the unknown Stem cell field is still in its infancy Human embryonic stem cell research is a decade old, adult stem cell

More information

Reprogramming, Screening and Validation of ipscs and Terminally Differentiated Cells using the qbiomarker PCR Array System

Reprogramming, Screening and Validation of ipscs and Terminally Differentiated Cells using the qbiomarker PCR Array System Reprogramming, Screening and Validation of ipscs and Terminally Differentiated Cells using the qbiomarker PCR Array System Outline of Webinar What are induced pluripotent Stem Cells (ips Cells or ipscs)?

More information

Human cord blood reprogrammed into embryonic-like stem cells

Human cord blood reprogrammed into embryonic-like stem cells STATE OF THE ART 3B-PL2 ISBT Science Series (2011) 6, 107 111 ª 2011 The Author(s). ISBT Science Series ª 2011 International Society of Blood Transfusion Human cord blood reprogrammed into embryonic-like

More information

Stem Cells and Inner Ear Cell Regeneration. Stefan Heller Stanford University School of Medicine

Stem Cells and Inner Ear Cell Regeneration. Stefan Heller Stanford University School of Medicine Stem Cells and Inner Ear Cell Regeneration Stefan Heller Stanford University School of Medicine Embryonic stem cells Induced pluripotent stem cells Somatic stem cells 50 µm Derived from the inner cell

More information

Stem cells possess 2 main characteristics: Sources of pluripotent stem cells: -Long-term self renewal. -The inner cell mass of the blastocyst.

Stem cells possess 2 main characteristics: Sources of pluripotent stem cells: -Long-term self renewal. -The inner cell mass of the blastocyst. Stem cells possess 2 main characteristics: -Long-term self renewal. - They give rise to all types of differentiate cells. Sources of pluripotent stem cells: -The inner cell mass of the blastocyst. - Fetal

More information

BioResearch. Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research

BioResearch. Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research BioResearch Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research BioResearch Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research Working with

More information

International Stem Cell Registry

International Stem Cell Registry International Stem Cell Registry Importance of Stem Cells Stem cells are model systems for the study of development and disease. Pluripotent stem cells offer new tools for drug design and discovery. Pluripotent

More information

Stem Cell Research Tools for Biologically Relevant Results

Stem Cell Research Tools for Biologically Relevant Results Stem Cell Research Tools for Biologically Relevant Results Stem Cell Products It All Stems from Here Simplify Your Reprogramming, Proliferation I can t find a reliable solution for generation and expansion

More information

Stem cell research solutions and support

Stem cell research solutions and support R&D Live chat tech tips online forums protocols FIELD APPLICATION SPECIALISTS posters webinars Stem cell research solutions and support More resources than any other supplier Stem cell research can be

More information

STEM CELL FELLOWSHIP

STEM CELL FELLOWSHIP Module I: The Basic Principles of Stem Cells 1. Basics of Stem Cells a. Understanding the development of embryonic stem cells i. Embryonic stem cells ii. Embryonic germ cells iii. Differentiated stem cell

More information

Cord derived MSC-Like Placenta derived Membranes- Matrix- Cells- MSC-like,

Cord derived MSC-Like Placenta derived Membranes- Matrix- Cells- MSC-like, Bank Public/Pvt Bank other cells Ship and distribute Collect and process other samples Support development of novel uses One cord Blood Unit Non stem cells RBC s Plateletes T B Monocytes NK cells Stem

More information

Stem Cells Market Trends based on Primary Industry Analysis

Stem Cells Market Trends based on Primary Industry Analysis GENReports: Market & Tech Analysis Stem Cells Market Trends based on Primary Industry Analysis > Enal Razvi, Ph.D. Biotechnology Analyst, Managing Director SELECTBIO US enal@selectbio.us > Gary Oosta,

More information

Embryonic & and induced pluripotent Stem Cells. May 2010 Dipl. Biol. Dr. Kurt Pfannkuche

Embryonic & and induced pluripotent Stem Cells. May 2010 Dipl. Biol. Dr. Kurt Pfannkuche Embryonic & and induced pluripotent Stem Cells May 2010 Dipl. Biol. Dr. Kurt Pfannkuche Seite 2 What makes a stem cell? open chromatin structure plasticity! Seite 3 self-renewal Plasticity Seite 4 Culture

More information

An Introduction to Stem Cell Biology. Michael L. Shelanski, MD,PhD Professor of Pathology and Cell Biology Columbia University

An Introduction to Stem Cell Biology. Michael L. Shelanski, MD,PhD Professor of Pathology and Cell Biology Columbia University An Introduction to Stem Cell Biology Michael L. Shelanski, MD,PhD Professor of Pathology and Cell Biology Columbia University Figures adapted from ISSCR. Presentations of Drs. Martin Pera (Monash University),

More information

Chapter 16 Reproductive Technology, Gene Therapy, and Stem Cells (modified)

Chapter 16 Reproductive Technology, Gene Therapy, and Stem Cells (modified) Chapter 16 Reproductive Technology, Gene Therapy, and Stem Cells (modified) Assisted Reproductive Technologies (ART) Artificial insemination (AI) In vitro fertilization (IVF) Gamete intrafallopian transfer

More information

COLUMBIA UNIVERSITY POLICY ON THE CONDUCT OF RESEARCH WITH HUMAN EMBRYOS AND HUMAN EMBRYONIC STEM CELLS*

COLUMBIA UNIVERSITY POLICY ON THE CONDUCT OF RESEARCH WITH HUMAN EMBRYOS AND HUMAN EMBRYONIC STEM CELLS* July 7, 2014 COLUMBIA UNIVERSITY POLICY ON THE CONDUCT OF RESEARCH WITH HUMAN EMBRYOS AND HUMAN EMBRYONIC STEM CELLS* A. INTRODUCTION Columbia University believes that human embryonic and human stem cell

More information

STEM CELL FACTS. The ISSCR is an independent, nonproft organization providing a global forum for stem cell research and regenerative medicine.

STEM CELL FACTS. The ISSCR is an independent, nonproft organization providing a global forum for stem cell research and regenerative medicine. STEM CELL FACTS The ISSCR is an independent, nonproft organization providing a global forum for stem cell research and regenerative medicine. WHAT ARE STEM CELLS? Stem cells are the foundation cells for

More information

Introducing stem cells

Introducing stem cells Introducing stem cells 1 Dear speaker This presentation is intended as a flexible tool for scientists, science communicators and educators. Not all the slides will be useful for any one occasion. Choose

More information

Please visit this site for the current GESCR procedures info: http://www.research.ucsf.edu/chr/stemcell/scprocedures.asp

Please visit this site for the current GESCR procedures info: http://www.research.ucsf.edu/chr/stemcell/scprocedures.asp GESCR PROCEDURES: REVIEW PROCESS AND LEVELS OF REVIEW Office of Origin: Human Research Protection Program Effective Date: January 2012 I. Introduction The Human Gamete, Embryo and Stem Cell Research (GESCR)

More information

Stem Cell Basics. About this document. I. Introduction: What are stem cells, and why are they important?

Stem Cell Basics. About this document. I. Introduction: What are stem cells, and why are they important? Stem Cell Basics Last modified on April 08, 2015 About this document This primer on stem cells is intended for anyone who wishes to learn more about the biological properties of stem cells, the important

More information

Culturing Embryonic and Adult-Derived Stem Cells: Introduction and Key Applications. Amy Laws, Ph.D. November 2008

Culturing Embryonic and Adult-Derived Stem Cells: Introduction and Key Applications. Amy Laws, Ph.D. November 2008 Culturing Embryonic and Adult-Derived Stem Cells: Introduction and Key Applications Amy Laws, Ph.D. November 2008 Outline Introduction to stem cells Embryonic stem cells Alternatives to embryonic stem

More information

Roche Position on Human Stem Cells

Roche Position on Human Stem Cells Roche Position on Human Stem Cells Background Stem cells and treating diseases. Stem cells and their applications offer an enormous potential for the treatment and even the cure of diseases, along with

More information

Biotechnology. Srivatsan Kidambi, Ph.D.

Biotechnology. Srivatsan Kidambi, Ph.D. Stem Stem Cell Cell Engineering-What, Biology and it Application Why, How?? to Biotechnology Srivatsan Kidambi, Ph.D. Assistant Professor Department of Chemical & Biomolecular Engineering University of

More information

guides BIOLOGY OF AGING STEM CELLS An introduction to aging science brought to you by the American Federation for Aging Research

guides BIOLOGY OF AGING STEM CELLS An introduction to aging science brought to you by the American Federation for Aging Research infoaging guides BIOLOGY OF AGING STEM CELLS An introduction to aging science brought to you by the American Federation for Aging Research WHAT ARE STEM CELLS? Stem cells are cells that, in cell cultures

More information

Guidance For Research Involving Human Embryonic Stem Cells, Germ Cells, And Cells Obtained From Cord Blood

Guidance For Research Involving Human Embryonic Stem Cells, Germ Cells, And Cells Obtained From Cord Blood Guidance For Research Involving Human Embryonic Stem Cells, Germ Cells, And Cells Obtained From Cord Blood Supreme Council of Health Department of Research Guidance Regarding Research Involving Human Embryonic

More information

Reflection paper on stem cell-based medicinal products

Reflection paper on stem cell-based medicinal products 14 January 2011 EMA/CAT/571134/2009 Committee for Advanced Therapies (CAT) Draft Agreed by Biologics Working Party (BWP) and Safety Working Party (SWP) March 2010 Draft Agreed by Cell Products Working

More information

The ethics of stem cell research and treatment

The ethics of stem cell research and treatment The ethics of stem cell research and treatment Bernard Lo, M.D. March 12, 2009 1 hesc: ethical controversies Moral status of embryo? Clearly a potential person Some believe a person with rights Is hesc

More information

The Types of stem cells: Totipotent Pluripotent Multipotent

The Types of stem cells: Totipotent Pluripotent Multipotent Stem Cells is the main material for building and regeneration of the body Stem cells are not differentiated and can transform to any cell of organism Stem cells are capable of indefinite renewal through

More information

Stem Cell Research: Adult or Somatic Stem Cells

Stem Cell Research: Adult or Somatic Stem Cells Chiang 1 Stem Cell Research: Adult or Somatic Stem Cells Abstract Kelly Chiang Cluster 7 Dr. LeFebvre 07/26/10 Over the past few decades, stem cells have been a controversial topic in the scientific field.

More information

Chapter 2 The different types of stem cells

Chapter 2 The different types of stem cells Chapter 2 The different types of stem cells This chapter investigates the two main types of stem cells, each with their own subcategories: 1. Pluripotent stem cells can become any cell type in the body.

More information

Stem Cell Research Source. From Isolation to Culture and Analysis

Stem Cell Research Source. From Isolation to Culture and Analysis Stem Cell Research Source From Isolation to Culture and Analysis Few scientific breakthroughs have sparked as much interest, imagination, and hope as the isolation and successful in vitro culture of human

More information

Generation and Culture of Neural Progenitor Cells using the STEMdiff Neural System

Generation and Culture of Neural Progenitor Cells using the STEMdiff Neural System Generation and Culture of Neural Progenitor Cells using the STEMdiff Neural System i Table of Contents 1.0 Introduction... 1 2.0 Materials, Reagents and Equipment... 2 2.1 Materials Required for Neural

More information

Stem Cell Information

Stem Cell Information Stem Cell Information The official National Institutes of Health resource for stem cell research Stem Cell Basics Stem cells have the remarkable potential to develop into many different cell types in the

More information

5 Frequently Asked Questions About Adult Stem Cell Research

5 Frequently Asked Questions About Adult Stem Cell Research 5 Frequently Asked Questions About Adult Stem Cell Research Stem cells are often referred to in the sociopolitical realm with some level of controversy and beyond that, some level of confusion. Many researchers

More information

The Therapeutic Potential of Human Umbilical Cord Blood Transplantation for Neonatal Hypoxic-Ischemic Brain Injury and Ischemic Stroke

The Therapeutic Potential of Human Umbilical Cord Blood Transplantation for Neonatal Hypoxic-Ischemic Brain Injury and Ischemic Stroke The Therapeutic Potential of Human Umbilical Cord Blood Transplantation for Neonatal Hypoxic-Ischemic Brain Injury and Ischemic Stroke a,b* b,c a a b b b b a b a b c 430 Wang et al. Acta Med. Okayama Vol.

More information

AP BIOLOGY 2012 SCORING GUIDELINES

AP BIOLOGY 2012 SCORING GUIDELINES AP BIOLOGY 2012 SCORING GUIDELINES Question 1 Note: At least 1 point must be earned from each of parts (a), (b), (c), and (d) in order to earn a maximum score of 10. The ability to reproduce is a characteristic

More information

BIOINFORMANT: Stem Cell Research Products: Opportunities, Tools & Technologies

BIOINFORMANT: Stem Cell Research Products: Opportunities, Tools & Technologies B. Breakdown of Stem Cell Research, by Cell Type Research supply companies preparing to launch a stem product line or expand an existing one are likely considering several stem cell types for use in product

More information

CD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors

CD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors CD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors Yajuan Jiang 1, Alexander M. Tsankov 2, Alexander Meissner 2 and David W. O

More information

Efficient human ips cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures

Efficient human ips cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures ORIGINAL ARTICLE Cell Research (2011) :1-12. 2011 IBCB, SIBS, CAS All rights reserved 1001-0602/11 www.nature.com/cr npg Efficient human ips cell derivation by a non-integrating plasmid from blood cells

More information

Should Stem Cells Be Used To Treat Human Diseases?

Should Stem Cells Be Used To Treat Human Diseases? SAMPLE ESSAY C Should Stem Cells Be Used To Treat Human Diseases? Stem cells can be defined as undifferentiated cells that are generated during the development of the embryo. There are two functions ascribed

More information

Are Muslims into stem cell research? Review of current available literature

Are Muslims into stem cell research? Review of current available literature Are Muslims into stem cell research? Review of current available literature Maysa Al-Hussaini and Linda Kateb King Hussein Cancer Center Amman Jordan No Disclosure What are the types of human stem cells?

More information

11/2011. Duke University Stem Cell Research Oversight Committee

11/2011. Duke University Stem Cell Research Oversight Committee 11/2011 Duke University Stem Cell Research Oversight Committee Duke University Stem Cell Research Oversight Committee The Cell Therapy Research Oversight Committee has proposed that a specialized review

More information

Stem cells and motor neurone disease

Stem cells and motor neurone disease Stem cells and motor neurone disease F Stem cell research has fuelled hope of a treatment for a variety of conditions. This information sheet explains what these cells are and includes details of the current

More information

Stem cells and motor neurone disease

Stem cells and motor neurone disease Stem cells and motor neurone disease F Stem cell research has fuelled hope of a treatment for a variety of conditions. This information sheet explains what these cells are and how they may be used to create

More information

15 Stem Cell Research

15 Stem Cell Research 15 Stem Cell Research t a l k it o v e r 1 c l a s s se s s i o n Overview Students read about current scientific research on and the social controversy over embryonic stem cells. On a KWL literacy strategy

More information

Stem Cells: Scientific Progress and Future Research Directions

Stem Cells: Scientific Progress and Future Research Directions 2001 Terese Winslow Stem Cells: Scientific Progress and Future Research Directions STEM CELLS: SCIENTIFIC PROGRESS AND FUTURE RESEARCH DIRECTIONS June 2001 This page intentionally left blank TABLE OF CONTENTS

More information

Equine Stem Cells. Sources, Processing, Expansion, Storage and Shipping

Equine Stem Cells. Sources, Processing, Expansion, Storage and Shipping Equine Stem Cells Sources, Processing, Expansion, Storage and Shipping Sean Owens, DVM, Diplomate ACVP Medical Director, UC Davis Veterinary Regenerative Medicine Laboratory What is a Stem Cell and What

More information

Cerebral Palsy. Information for patients. Cerebral Palsy Treatment With Stem Cells from your own Bone Marrow / Adipose Tissue

Cerebral Palsy. Information for patients. Cerebral Palsy Treatment With Stem Cells from your own Bone Marrow / Adipose Tissue Information for patients Cerebral Palsy Treatment With Stem Cells from your own Bone Marrow / Adipose Tissue Cerebral Palsy Cerebral palsy is a neurological impairment principally causing loss or limitation

More information

Current Development and Ethical Issues in Stem Cell Research and Applications

Current Development and Ethical Issues in Stem Cell Research and Applications Current Development and Ethical Issues in Stem Cell Research and Applications Pang-Chui Shaw ( 邵 鵬 柱 ) Biochemistry Programme School of Life Sciences Chinese University of Hong Kong January 25, 2014 Contents

More information

[Indian Stem Cell Banking Market: Trends & Opportunities (2012-2017)]

[Indian Stem Cell Banking Market: Trends & Opportunities (2012-2017)] [Indian Stem Cell Banking Market: Trends & Opportunities (2012-2017)] Executive Summary Stem Cells have a remarkable ability to develop in various body cells of a human. These occur in the earliest stages

More information

47/CNECV/05 STEM CELL RESEARCH

47/CNECV/05 STEM CELL RESEARCH CONSELHO NACIONAL DE ÉTICA PARA AS CIÊNCIAS DA VIDA NATIONAL COUNCIL OF ETHICS FOR THE LIFE SCIENCES 47/CNECV/05 OPINION Nº 47 OF THE NATIONAL COUNCIL OF ETHICS FOR THE LIFE SCIENCES STEM CELL RESEARCH

More information

Human stem cell research: a novel technology that will benefit patients Information and Position Paper

Human stem cell research: a novel technology that will benefit patients Information and Position Paper October 2003 Human stem cell research: a novel technology that will benefit patients Information and Position Paper EuropaBio, the European Association for Bio-industries, has 35 corporate members operating

More information

Biobanking Pluripotent Stem Cell Lines: UK and EU regulations Glyn Stacey, UK Stem Cell Bank, NIBSC

Biobanking Pluripotent Stem Cell Lines: UK and EU regulations Glyn Stacey, UK Stem Cell Bank, NIBSC Biobanking Pluripotent Stem Cell Lines: UK and EU regulations Glyn Stacey, UK Stem Cell Bank, NIBSC ISCT Conference, New Zealand, April 2013 April 1 st 2013 NIBSC will become part of the Medicines and

More information

Mesenchymal Stem Cells

Mesenchymal Stem Cells Mesenchymal Stem Cells Instruction Manual Product Size Catalog Number from Bone Marrow (hmsc-bm) from Umbilical Cord Matrix (hmsc-uc) from Adipose Tissue (hmsc-at) 500,000 cryopreserved cells 500,000 proliferating

More information

Stem Cells. Part 1: What is a Stem Cell?

Stem Cells. Part 1: What is a Stem Cell? Stem Cells Part 1: What is a Stem Cell? Stem cells differ from other kinds of cells in the body. When a stem cell divides by mitosis, each new cell has the potential to either remain a stem cell or become

More information

BioResearch. RAFT 3D Cell Culture Kit Protocol

BioResearch. RAFT 3D Cell Culture Kit Protocol BioResearch RAFT 3D Cell Culture Kit Protocol RAFT 3D Cell Culture Kit This protocol describes in detail how to produce RAFT 3D Cell Cultures in 96-well plates, 24-well plates and 24-well inserts. It is

More information

Mouse ES Cell Nucleofector Kit

Mouse ES Cell Nucleofector Kit page 1 of 7 Mouse ES Cell Nucleofector Kit for Mouse Embryonic Stem Cells Cell type Origin Cells derived from mouse blastocysts. Morphology Round cells growing in clumps. Important remarks! 1. This protocol

More information

Embryo Clay Model Embryogenesis and Stem Cell Development

Embryo Clay Model Embryogenesis and Stem Cell Development Embryo Clay Model Embryogenesis and Stem Cell Development Objective: To identify stages and locations of early embryonic development To see at what point stem cells are totipotent, pluripotent, and multipotent

More information

STEM CELLS FROM THE UMBLICAL CORD BLOOD AND UMBLICAL CORD TISSUE

STEM CELLS FROM THE UMBLICAL CORD BLOOD AND UMBLICAL CORD TISSUE STEM CELLS FROM THE UMBLICAL CORD BLOOD AND UMBLICAL CORD TISSUE What are Stem Cells? Stem cells are the basic building blocks of all the cells, tissues and organs in the human body. The role of the stem

More information

Unit 1 Higher Human Biology Summary Notes

Unit 1 Higher Human Biology Summary Notes Unit 1 Higher Human Biology Summary Notes a. Cells tissues organs body systems Division of labour occurs in multicellular organisms (rather than each cell carrying out every function) Most cells become

More information

April 2010 Code of Practice for the use of Human Stem Cell Lines

April 2010 Code of Practice for the use of Human Stem Cell Lines April 2010 Code of Practice for the use of Human Stem Cell Lines Document3\16/04/10Teladia Page 1 Foreword This Code provides guidance on best practice for those working with stem cell lines and specifies

More information

Stem Cell Therapy. THE DARK AND BRIGHT ASPECTS of Stem Cell Therapy

Stem Cell Therapy. THE DARK AND BRIGHT ASPECTS of Stem Cell Therapy Stem Cell Therapy THE DARK AND BRIGHT ASPECTS of Stem Cell Therapy Stem Cells Our last Weapon? The last weapon in the medical armamentarium? Bye the way.. What IS a last weapon? I don t know about today,

More information

THE FUTURE OF STEM CELLS IN MEDICINE BY AGNES MILNER KATIE CLARK. Pass with Merit

THE FUTURE OF STEM CELLS IN MEDICINE BY AGNES MILNER KATIE CLARK. Pass with Merit THE FUTURE OF STEM CELLS IN MEDICINE BY AGNES MILNER KATIE CLARK Pass with Merit RESEARCH PAPER BASED ON PATHOLOGY LECTURES AT MEDLINK 2011 1 Abstract Embryonic stem cell research offers great promise

More information

Stem Cell Research. Analysis and Isolation of Stem Cells Using Flow Cytometry

Stem Cell Research. Analysis and Isolation of Stem Cells Using Flow Cytometry Stem Cell Research Analysis and Isolation of Stem Cells Using Flow Cytometry 2 Cover Illustration: Fairman Studios, LLC. Flow cytometry is a powerful methodology for characterizing, analyzing, and isolating

More information

Cord Blood Re-imagined The use of cord blood stem cells for clinical applications and medical innovations

Cord Blood Re-imagined The use of cord blood stem cells for clinical applications and medical innovations GE Healthcare Proceedings of the Symposium Cord Blood Re-imagined The use of cord blood stem cells for clinical applications and medical innovations 12 th Annual Meeting of the International Society for

More information

Stem Cell Basics for Life Science Researchers

Stem Cell Basics for Life Science Researchers Stem Cell Basics for Life Science Researchers imaging and analysis repeatable and reproducible and maximize data quality custom analysis reports to make analysis automated, easy-to-use, and fast Create

More information

Amaxa Mouse T Cell Nucleofector Kit

Amaxa Mouse T Cell Nucleofector Kit Amaxa Mouse T Cell Nucleofector Kit For T cells isolated from C57BL/6 & BALB/c mice Evaluated for murine T cells isolated from C57BL/6 & BALB/c mice This protocol is designed for murine lymphocytes or

More information

Stem cells in regenerative medicine: introduction

Stem cells in regenerative medicine: introduction Published Online May 11, 2011 Stem cells in regenerative medicine: introduction Dusko Ilic 1, and Julia M. Polak 2* 1 Kings College London School of Medicine, Division of Women s Health, Assisted Conception

More information

Stem Cell Quick Guide: Stem Cell Basics

Stem Cell Quick Guide: Stem Cell Basics Stem Cell Quick Guide: Stem Cell Basics What is a Stem Cell? Stem cells are the starting point from which the rest of the body grows. The adult human body is made up of hundreds of millions of different

More information

Primary evaluation of induced pluripotent stem cells using flow cytometry

Primary evaluation of induced pluripotent stem cells using flow cytometry 3 Special Issue: Mesenchymal stem cells Original Article Primary evaluation of induced pluripotent stem cells using flow cytometry Daisuke Araki 1,2, ), Yoshimi Kawamura 2, ), Kunimichi Niibe 1,2), Sadafumi

More information

Human Umbilical Cord-derived Multipotent Mesenchymal Stromal Cells (huc-msc) Handling Instructions

Human Umbilical Cord-derived Multipotent Mesenchymal Stromal Cells (huc-msc) Handling Instructions Human Umbilical Cord-derived Multipotent Mesenchymal Stromal Cells (huc-msc) Order No.: 19401-005, 19401-010 Handling Instructions For preclinical ex vivo use. Not intended for therapeutic use. Table of

More information

Fee-for-service cores for Investigational New Drug enabling studies

Fee-for-service cores for Investigational New Drug enabling studies UC DAVIS INSTITUTE FOR REGENERATIVE CURES Fee-for-service cores for Investigational New Drug enabling studies Pipeline pathway at UC Davis Institute for Regenerative Cures Basic Research Proof of Concept

More information

Stem cell research ACADEMY STATEMENT. progress, hopes and concerns ACADEMY STATEMENT 16 JANUARY 2013

Stem cell research ACADEMY STATEMENT. progress, hopes and concerns ACADEMY STATEMENT 16 JANUARY 2013 ACADEMY STATEMENT 16 JANUARY 2013 ACADEMY STATEMENT Stem cell research progress, hopes and concerns KUNGL. VETENSKAPSAKADEMIEN, BOX 50005, SE-104 05 STOCKHOLM, SWEDEN TEL +46 8 673 95 00, FAX +46 8 15

More information

Registries and Biobanks for Human Stem Cell Lines Glyn Stacey, UK Stem Cell Bank, NIBSC ESHRE Course, Valencia, 8 th November 2010

Registries and Biobanks for Human Stem Cell Lines Glyn Stacey, UK Stem Cell Bank, NIBSC ESHRE Course, Valencia, 8 th November 2010 Registries and Biobanks for Human Stem Cell Lines Glyn Stacey, UK Stem Cell Bank, NIBSC ESHRE Course, Valencia, 8 th November 2010 National Institute for Biological Standards and Control Assuring the quality

More information

MEF Nucleofector Kit 1 and 2

MEF Nucleofector Kit 1 and 2 page 1 of 7 MEF Nucleofector Kit 1 and 2 for Mouse Embryonic Fibroblasts (MEF) MEF display significant phenotypic variations which depend on the strain, the genetic background of the they are isolated

More information

INFORMATION ON POTENTIAL RESTRICTIONS OR LICENSE REQUIREMENTS FOR THE USE OF REPROGRAMMED CELLS

INFORMATION ON POTENTIAL RESTRICTIONS OR LICENSE REQUIREMENTS FOR THE USE OF REPROGRAMMED CELLS INFORMATION ON POTENTIAL RESTRICTIONS OR LICENSE REQUIREMENTS FOR THE USE OF REPROGRAMMED CELLS In addition to the Terms contained in the National Institute of Mental Health (NIMH) Repository and Genomics

More information

Amaxa 4D-Nucleofector Protocol for Mouse Embryonic Stem [ES] Cells For 4D-Nucleofector X Unit Transfection in suspension

Amaxa 4D-Nucleofector Protocol for Mouse Embryonic Stem [ES] Cells For 4D-Nucleofector X Unit Transfection in suspension For 4D-Nucleofector X Unit Transfection in suspension Cells derived from mouse blastocysts; round cells, growing in clumps s 1. This protocol is meant to provide an outline for the handling and the Nucleofection

More information

56 MaxPlanckForschung 3 09

56 MaxPlanckForschung 3 09 56 MaxPlanckForschung 3 09 One factor is enough for neural stem cells to become pluripotent. They can then be differentiated into smooth muscular cells that are found, for example, in blood and lymph vessels.

More information

Published by: Director General Indian Council of Medical Research New Delhi 110029. December 2013. Cover page created by: Dr.

Published by: Director General Indian Council of Medical Research New Delhi 110029. December 2013. Cover page created by: Dr. Published by: Director General Indian Council of Medical Research New Delhi 110029 December 2013 Cover page created by: Dr. Geeta Jotwani Foreword i Acknowledgement iii Abbreviations iv 1.0 Preamble 1

More information

Technology Analysis and Market Outlook

Technology Analysis and Market Outlook Investing in Regenerative Medicine: Technology Analysis and Market Outlook 2014 Report #1 ISBN # 978-0-9912902-1-5 May 2014 Table of Contents Glossary...2 Introduction... 4 Regenerative Medicine Landscape...

More information

What are Stem Cells? How can they be used in medicine?

What are Stem Cells? How can they be used in medicine? What are Stem Cells? How can they be used in medicine? What is a stem cell????... What is a stem cell????......a cell with the ability to differentiate into specialized cells and renew to become more stem

More information

Reprogramming hierarchy in mouse liver cell subpopulations depends on cell intrinsic factors including BAF complex members.

Reprogramming hierarchy in mouse liver cell subpopulations depends on cell intrinsic factors including BAF complex members. Reprogramming hierarchy in mouse liver cell subpopulations depends on cell intrinsic factors including BAF complex members. A dissertation submitted to the faculty of natural sciences for the requirement

More information

MEF Starter Nucleofector Kit

MEF Starter Nucleofector Kit page 1 of 7 MEF Starter Nucleofector Kit for Mouse Embryonic Fibroblasts (MEF) MEF display significant phenotypic variations which depend on the strain, the genetic background of the mice they are isolated

More information

Von Mäusen und Menschen E - 1

Von Mäusen und Menschen E - 1 Von Mäusen und Menschen E - 1 Mus musculus: Genetic Portrait of the House Mouse E - 3 Outline Mouse genome Mouse life cycle Transgenic protocols Addition of genes by nuclear injection Removal of genes

More information

Stem Cell Banking & Application: Guidelines & Regulatory Framework

Stem Cell Banking & Application: Guidelines & Regulatory Framework Stem Cell Banking & Application: Guidelines & Regulatory Framework Dr. Geeta Jotwani Assistant Director General Indian Council of Medical Research Department of Health Research (MoHFW) New Delhi, India

More information

Chapter 47: Animal Development

Chapter 47: Animal Development Name Period Overview 1. An organism s development is controlled by the genome of the zygote as well as by molecules from the mother that are in the cytoplasm of the egg. What are these proteins and RNAs

More information

Current Issues in Stem Cell Technologies. Lance D. Trainor, MD OneBlood, Inc.

Current Issues in Stem Cell Technologies. Lance D. Trainor, MD OneBlood, Inc. Current Issues in Stem Cell Technologies Lance D. Trainor, MD OneBlood, Inc. Objective: The big picture of stem cell therapy Outline: Relevant definitions History of Stem Cell Therapy Hematopoietic Stem

More information

Induced pluripotent stem cells: An update

Induced pluripotent stem cells: An update Singh et al. 6 Original REview article Peer Reviewed OPEN ACCESS Induced pluripotent stem cells: An update Anubha Singh, Deepak Kumar Singh, Usha Bhoria Abstract The discovery that the mature cells can

More information

Contents. Foreword. www.esf.org. Human Stem Cell Research and Regenerative Medicine A European Perspective on Scientific, Ethical and Legal Issues

Contents. Foreword. www.esf.org. Human Stem Cell Research and Regenerative Medicine A European Perspective on Scientific, Ethical and Legal Issues 38 SCIENCE POLICY BRIEFING May 2010 Human Stem Cell Research and Regenerative Medicine A European Perspective on Scientific, Ethical and Legal Issues Contents 1 Foreword 2 Introduction 2 Foetal stem cells

More information

Role Of Mitochondria In Mesenchymal Stem Cells

Role Of Mitochondria In Mesenchymal Stem Cells Role Of Mitochondria In Mesenchymal Stem Cells Roman Eliseev, MD, PhD, Tamara Raymond, Regis O'Keefe, MD, PhD. University of Rochester, Rochester, NY, USA. Disclosures: R. Eliseev: None. T. Raymond: None.

More information

Support structure for genetic material

Support structure for genetic material Support structure for genetic material 1 Making proteins in the RER Making copies of humans 2 Making copies of cells Making copies of genetic material 3 Making copies of genetic material Making copies

More information

The Prospect of Stem Cell Therapy in Multiple Sclerosis. Multiple sclerosis is a multifocal inflammatory disease of the central

The Prospect of Stem Cell Therapy in Multiple Sclerosis. Multiple sclerosis is a multifocal inflammatory disease of the central The Prospect of Stem Cell Therapy in Multiple Sclerosis Multiple sclerosis is a multifocal inflammatory disease of the central nervous system that generally affects young individuals, causing paralysis

More information

RUCDR Infinite Biologics Cell Culture and Stem Cell Center

RUCDR Infinite Biologics Cell Culture and Stem Cell Center RUCDR Infinite Biologics Cell Culture and Stem Cell Center 10 MECHANICAL STORAGE (-70 C AND -80 C) CELL CULTURE LABORATORY 7 CELL LINE FREEZING/STORAGE 6 CELL LINE EXPANSION/ 4 SAMPLE ACCESSIONING MAINTENANCE

More information

NEURAL STEM CELL PRODUCTS. Neural Stem Cells. Standardized Media and Reagents. Table of Contents

NEURAL STEM CELL PRODUCTS. Neural Stem Cells. Standardized Media and Reagents. Table of Contents NEURAL STEM CELL PRODUCTS Neural Stem Cells Standardized Media and Reagents Table of Contents Table of Contents 3 4 6 8 12 13 13 14 14 Superior Neural Stem Cell Culture with Consistent, High-Quality Media

More information

Guideline for Generation of Stable Cell Lines Technical Reference Guide

Guideline for Generation of Stable Cell Lines Technical Reference Guide BioResearch Guideline for Generation of Stable Cell Lines Technical Reference Guide 1. Background Stable, long-term expression of a gene of interest can be either achieved by eukaryotic vectors that harbor

More information

PART I. SECTION 1. The legislature finds that an estimated. 128,000,000 Americans suffer from the crippling economic and

PART I. SECTION 1. The legislature finds that an estimated. 128,000,000 Americans suffer from the crippling economic and HOUSE OF REPRESENTATIVES TWENTY-FOURTH LEGISLATURE, 2007 STATE OF HAWAII A BILL FOR AN ACT RELATING TO MEDICAL RESEARCH. BE IT ENACTED BY THE LEGISLATURE OF THE STATE OF HAWAII: PART I SECTION 1. The legislature

More information

San Diego Stem Cell Treatment Center Frequently Asked Questions

San Diego Stem Cell Treatment Center Frequently Asked Questions San Diego Stem Cell Treatment Center Frequently Asked Questions What is a Stem Cell? A stem cell is basically any cell that can replicate and differentiate. This means the cell can not only multiply, but

More information

Stem Cell Research in India: Emerging Scenario and Policy Concerns

Stem Cell Research in India: Emerging Scenario and Policy Concerns RIS Research and Information System for Developing Countries www.ris.org.in/abdr.html Asian Biotechnology and Development Review Vol. 8 No. 3, pp 43-53 2006, RIS. All rights reserved 43 Printed in India

More information